Search found 90 matches

by Raul
Wed Jan 27, 2016 12:02 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 3030

Re: Unidentified

Well zzffnn and gekko, it is a wet mount, also I added more water to the slide and nothing ( so not a crystal of m.b.). It appears to be stucked to the slide ( I fixed the cells with a lighter). The thing is the slide was pretty normal ( muscles cells, epithelial cells) and all of a sudden this. I v...
by Raul
Tue Jan 26, 2016 8:53 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 3030

Unidentified

Hi there I just found this while centrifuging a blood sample from a beef. It is stained with methylene blue and is around 35 microns. Does anyone have an idea of what it could be?
by Raul
Mon Jan 25, 2016 8:57 pm
Forum: Beginner's corner
Topic: Safranin working solution?
Replies: 6
Views: 1611

Re: Safranin working solution?

Thank you 75RR for the link and thank you Peter for telling me the concentration and type of solution.

Best regards my friends.
by Raul
Mon Jan 25, 2016 10:14 am
Forum: Beginner's corner
Topic: Safranin working solution?
Replies: 6
Views: 1611

Re: Safranin working solution?

Also if anyone can explain me what are the advantages and disadvantages of working with an aqueous solution or alcoholic solution please.
by Raul
Mon Jan 25, 2016 10:13 am
Forum: Beginner's corner
Topic: Safranin working solution?
Replies: 6
Views: 1611

Safranin working solution?

Hi guys, I recently bought Safranin as a powder, and I would like some advice on how to make the solution (if aqueous or alcoholic) what proportions should it have and what concentration of the dye. I am looking to do Gram stain but also general nuclear stains and cartilage stains. Please if you can...
by Raul
Wed Dec 02, 2015 10:21 pm
Forum: Pictures and Videos
Topic: Effect of digital enhancement - before and after
Replies: 15
Views: 1484

Re: Effect of digital enhancement - before and after

Very good and sharp image+video, also thank you for describing how you enhanced them. Keep up the good work.
by Raul
Sun Nov 29, 2015 1:08 pm
Forum: Pictures and Videos
Topic: What do I do with loads of sections?
Replies: 10
Views: 608

Re: What do I do with loads of sections?

Hi John B, beautiful desk you have there and very well organized, I especially like that you are meticulous with your work. Thank you for sharing your protocol and photographs. Best of luck.

P.S. by the way from where did you get formaldehyde in England ? :)

Thank you and keep up the good work.
by Raul
Thu Nov 26, 2015 11:20 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Re: Unknown organ identification, H&E stain

Hi charlie g thank you very much, indeed I learned a valuable lesson (not to say: o, I will identify this organ after the staining :D). Thank you for sharing that image, it was kinda the same that I saw and decided that the chicken will serve a even higher purpose for science (also cooking). Nonethe...
by Raul
Tue Nov 24, 2015 4:22 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Re: Unknown organ identification, H&E stain

I believe that as charlie g named it so poetic, Viscera unknown is a lymph node. I searched thorough a lot of histology book and all indicate this. It was hard to distinguish because my section was not sectioned at the proper size of a 6-10 μ, instead with my hand made microtome I think it is around...
by Raul
Mon Nov 23, 2015 9:26 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Re: Unknown organ identification, H&E stain

B zone + cells that were removed from the tissue (seem like epithelial cells),800x magnification of epithelial cells, 400x magnification of B zone
by Raul
Mon Nov 23, 2015 9:22 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Re: Unknown organ identification, H&E stain

A zone ; Magnifications in order of images:100x,200x,400x,400x with 2.5xzoom,800x, 800x with 2.5xzoom
by Raul
Mon Nov 23, 2015 9:21 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Re: Unknown organ identification, H&E stain

Additional photos.
by Raul
Sun Nov 22, 2015 7:30 pm
Forum: Identification help
Topic: Unknown organ identification, H&E stain
Replies: 9
Views: 2043

Unknown organ identification, H&E stain

I've been having a go at making my own histology slide, but I encountered an interesting problem. First, the organ tissue was taken from a frozen whole chicken bought from the supermarket, it still had both lungs,both kidneys and what I tought to be a blood vessel from one kidney, I will post pictur...
by Raul
Sat Nov 14, 2015 5:33 pm
Forum: Pictures and Videos
Topic: Colourful vessels from an old block
Replies: 16
Views: 655

Re: Colourful vessels from an old block

Fantastic pictures, book quality there :) I'm glad to see you're still excited by your work. Keep up the good work.

p.s. the methylene blue pictures seems to have a little metachromatic effect on it, do you know a method of emphasizing the effect?

Raul.
by Raul
Sat Oct 31, 2015 7:44 pm
Forum: Beginner's corner
Topic: Eosin proper concentration for histology?
Replies: 3
Views: 1241

Re: Eosin proper concentration for histology?

Thank you for your reply John B, it seems that 1% at 2 min does the job well compared to the rest. Thank you for your advice on Safranin and Orange G it may come in handy at later times :).
by Raul
Tue Oct 27, 2015 9:12 pm
Forum: Pictures and Videos
Topic: Red Grape
Replies: 9
Views: 777

Re: Red Grape

Beautiful :)
by Raul
Tue Oct 27, 2015 9:11 pm
Forum: Pictures and Videos
Topic: Pig Motor Nerve (4x, 10x, 40x)
Replies: 14
Views: 1367

Re: Pig Motor Nerve (4x, 10x, 40x)

Well it kinda looks to me like it, having dendrites, but the astrocytes are only present in the brain and spinal cord, it dependes from where this sample was taken.
by Raul
Tue Oct 27, 2015 8:40 pm
Forum: Beginner's corner
Topic: Eosin proper concentration for histology?
Replies: 3
Views: 1241

Eosin proper concentration for histology?

As the title says I want to know what the suitable concentration of Eosin would be for most histological applications. I checked on the internet and some proposed 1% other 0.5% and even 0.2% (of course the staining time differed with each concentration). I would wish to know from someone that had go...
by Raul
Tue Oct 27, 2015 8:35 pm
Forum: Pictures and Videos
Topic: Pig Motor Nerve (4x, 10x, 40x)
Replies: 14
Views: 1367

Re: Pig Motor Nerve (4x, 10x, 40x)

Incredible, thank you very much!
by Raul
Mon Oct 26, 2015 11:02 am
Forum: Beginner's corner
Topic: Recipe for a Romanowsky stain solution?
Replies: 4
Views: 1119

Re: Recipe for a Romanowsky stain solution?

Thank you 75RR, I did come across his name about some months ago but I totally forgot about him. Thanks for refreshing my memory.
by Raul
Mon Oct 26, 2015 11:00 am
Forum: Beginner's corner
Topic: Recipe for a Romanowsky stain solution?
Replies: 4
Views: 1119

Re: Recipe for a Romanowsky stain solution?

Thank you Seb28, the last link had some good information on quantities. I will experiment and if those look fine I will post some pictures:).
by Raul
Sun Oct 25, 2015 8:13 pm
Forum: Pictures and Videos
Topic: Skeletal Muscle (Pig), H & E stain, self made
Replies: 8
Views: 1394

Re: Skeletal Muscle (Pig), H & E stain, self made

gekko wrote:Nice work.
Thank you very much :).
by Raul
Sun Oct 25, 2015 8:11 pm
Forum: Pictures and Videos
Topic: Pig Motor Nerve (4x, 10x, 40x)
Replies: 14
Views: 1367

Re: Pig Motor Nerve (4x, 10x, 40x)

I think over there.
by Raul
Sun Oct 25, 2015 8:00 pm
Forum: Pictures and Videos
Topic: Pig Motor Nerve (4x, 10x, 40x)
Replies: 14
Views: 1367

Re: Pig Motor Nerve (4x, 10x, 40x)

What was it stained with? Also can you take a picture of an astrocyte (I think I can see one about 2/3 to the right-down corner). It is a very nice sample of tissue, a beautiful piece and a lot of work and trails. Please post more :).

Thank you for showing this.
by Raul
Sat Oct 24, 2015 7:24 pm
Forum: Beginner's corner
Topic: Recipe for a Romanowsky stain solution?
Replies: 4
Views: 1119

Recipe for a Romanowsky stain solution?

Hi guys I want to make the solution using methylene blue and eosin. I am interested in the proportions to use and also other aspects like buffers or oxidants for m.b.. I know that I can buy a cheap Giemsa stain, but I want to explore the science behind it.

Thank you.
by Raul
Sat Oct 24, 2015 6:44 pm
Forum: Pictures and Videos
Topic: Skeletal Muscle (Pig), H & E stain, self made
Replies: 8
Views: 1394

Re: Skeletal Muscle (Pig), H & E stain, self made

Another excellent picture - very nice indeed, I'd be very proud of my efforts if I'd created these images and the slide/s from which they are taken. Well done. :) Thank you very much, also you are a bit to blame John B :) that I bought H and E (giving me links to some sites, and more other advice)....
by Raul
Sat Oct 24, 2015 6:29 pm
Forum: Pictures and Videos
Topic: Skeletal Muscle (Pig), H & E stain, self made
Replies: 8
Views: 1394

Re: Skeletal Muscle (Pig), H & E stain, self made

Thank you very much for your kind words,mrsonchus and JimT, the magnification was 400x microscope and 2.5x from camera. I have another sample with a bigger camera zoom 3x( this time on purposely overstained with Mayer's Haematoxylin) to have a better visibility of the nuclei. Although the striations...
by Raul
Fri Oct 23, 2015 3:04 pm
Forum: Pictures and Videos
Topic: Skeletal Muscle (Pig), H & E stain, self made
Replies: 8
Views: 1394

Skeletal Muscle (Pig), H & E stain, self made

My first histological slide using H & E stain of pig skeletal muscle. It is mounted in glycerine-jelly (R.I.=1.47)
Hope you enjoy it.
by Raul
Fri Oct 23, 2015 12:26 pm
Forum: Pictures and Videos
Topic: Trichrome staining
Replies: 6
Views: 691

Re: Trichrome staining

Hi Naphthalene, congratulation for the samples, they are indeed with a lot of information and also they present a sweet for the eyes. I do have a question for you, did you post-process the images with a software (like photoshop or something similar) ?
by Raul
Sat Oct 17, 2015 7:09 pm
Forum: Pictures and Videos
Topic: A Bit of Manipulation
Replies: 27
Views: 1402

Re: A Bit of Manipulation

Wow mrsonchus you have a lot to show my friend. Please keep your microscopy passion so that we can enjoy the show :). Very nice images, I like the clarity in them, the information that you show and the simple protocols that work very well. Keep up the good work.

Kind regards, Raul.