MicrobeHunter.com Microscopy Forum

Very nice images, Hobbyst46! May I suggest use an objective hood (wrapped tightly around objective lens) made of Vellum paper for better difdusion? Even think office paper will work better than not having any diffusion. In you photo #19, I can see two hot spots, perpendicular to them, I can kind of ...

Dave, You can call me Fan (which is my first name). You may want to think about resale value, in case you don't like microscopy. A used Zeiss scope may be bought from a hobbyist for 300 GBP, and would retain its resale value much better than a used Chinese scope. Or buy a used Chinese scope at deep ...

Welcome to the forum, Dave. There are a few UK microscopy groups. You best option will be buying a quality used scope from group members and try it in person before buying: http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/amateurs/mainpeop.html Or go to a nearby surplus auc...

I don't know about IOR 20x. Most likely it is a dry objective. I have a LOMO water immersion 30x objective and saw your typographic error of "30x"; that was why I asked.

It is hard to say if Zeiss Pancratic condenser will fit on your scope. Pancratic condenser is quite tall and needs to be lowered enough to change its turret heads. So you need to measure the maximum under stage height available for a condenser. Better still, have right to return it if it does not fi...

David, Both of those will mechanically fit on your S, but am not sure they are complete and functional (for example, is the head prism still perfectly aligned?). Also some trinocular heads do not offer simultaneous visual and camera viewing (I don't know which is which). So it is probably better to ...

David, You can buy a teaching head attachment, then add a S series monocular head to one (of the two) head mount (the other head mount can receive a bino head) That would provide you a trinocular head too. Note there is a G series mono head with smaller (less desirable for photography) bottom lens. ...

2000x is always a joke. 1500x is about the max for a great NA 1.3 or 1.4 100x oil objective. Darkfield oil condenser needs immersion oil between its top lens and slide bottom. It needs almost perfect centration and should rise high enough to almost touching slide bottom. M4/3 camera - Sigma 30mm m4/...

Others objectives have been sold. The 70x is still available.

Adding a blue filter will correct color temperature, but it will dim light even more. T490 is known to have a decent focus mechanism, but it may have quite some plastic parts (? not sure, but I personally don't like AmScope as they are not easy to upgrade and resale value is low). You can spend abou...

^ You may want to specify what special features do you want out of a $1000 scope and changed your thread title accordingly. Most of the name brand scopes can do what you asked for in your first post, with afocal imaging (camera lens equivalent to 50-60mm full frame, over 10x visual eyepiece, for exa...

Most work of bacterial ID is not done by microscope. Take this from a microbiology PhD. Depending on how often you what to do the ID, you may be much better off sending samples to a commercial diagnostic lab and spend your $1k that way. May I ask why you want to do bacterial ID? There are many more ...

Pat, Inverting slide should work, if you squeeze out excessive water. If it still does not, please sell it to me for a low price :mrgreen: Main benefits of inverted scopes include: 1) subject falls onto covet slip because of gravity, so less water or culture media lies between subject and cover slip...

HMC condenser should have a slit like this: http://www.phasecontrastmicroscopes.com/hoffman-modulation-contrast-hmc-condenser-model-g3.php And condenser slit should align with objective slit for HMC (assuming all optics are present and functional). It would be nice to get 20x phase contrast, but you...

The Pv 10x is a phase contrast objective meant to be used with Leitz Heine variable phase condenser, if I remember correctly. Phase objectives don't work well for fluorescence.

Welcome to the forum, George! I have the same question about tube length. The tube length in that photo of yours may even exceed 210mm. I have not used any illumination-through-objective kind of true epi objectives. But you may be able to buy RMS adapters (such as 27mm-RMS) from eBay. LOMO epi objec...

Your IPC may not work with your 1.2 plan or 40x apo. But it does not hurt to try them all. Most likely, it will work with Mplan 10x and 20x. Many microscopist like using 20x and 40x objectives for pond protists. People interested in insects usually don't go over 20x (mostly 4x and 10x). But I use 65...

Forum member @SpocksSister
memberlist.php?mode=viewprofile&u=1366

Seems to have an IPC unit https://spockssister.com/category/micro ... on-s-l-ke/

Maybe she can comment as well?

PeteM, Author of my linked articles (Dave Jackson) has an IPC head unit, but I don't know if he has a matching IPC condenser. His intent is to use the IPC with better CFN objective, and he has Leitz Heine condenser, so he probably did not need or care for the matching IPC condenser. I have not seen ...

Welcome to the forum, Erik. Your 1.2 plan is an objective with 1.2x magnification and longer than usual working distance. Your stage may or may not go down enough for it to focus though. I have that 1.2 plan objective myself, which works on my Nikon Optiphot mk1. Your 5x, 10x, 15x and 20x eyepieces ...

I have for sale a LOMO apochromatic 70x NA 1.23 water Immersion phase contrast objective with cover slip correction collar (range is 0.10 mm to 0.20mm). Great condition. Tube length is 160 mm. $107 + actual shipping cost Collar is slightly stiff but will turn with normal force from fingers. Parfocal...

The main application of that LOMO water 40x 0.75 objective is dipping and patch clamp.

Welcome to the forum! Unless your wife is fine with a $3000 budget fot a complete DIC scope (in which case I recommend PZO or Zeiss, if you can find a Zeiss one without delamination), you can start with any brand. It would not hurt though, if you start with a PZO or Zeiss frame. Just know that PZO D...

I bought this for a lower price that listing and like it: https://www.amazon.com/TS1983-Focus-Scale-Magnification-Diameter/dp/B009NVF868/ref=asc_df_B009NVF868/?tag=hyprod-20&linkCode=df0&hvadid=312106929825&hvpos=1o1&hvnetw=g&hvrand=13192609385522361418&hvpone=&hvptwo=&hvqmt=&hvdev=m&hvdvcmdl=&hvloc...

Remember most parts from the **phots (Labophot, Optiphot, Diaphot, ect) from Nikon are newer ones and won't work on the older S or G series. The only exception is the heads, which are compatible. You may be able to use long 45mm parfocal objectives on your S model, but you would lose about 12mm of s...

Hi again and welcome!

So you don't use high power (compound) scopes for hobby?

For low power (up to 20x NA 0.4) darkfield, you can simply put on a black colored background and shine your lights sideways towards subject.

Some foram IDs can be found here: https://sites.google.com/site/rhowey/am ... ogofforams

http://www.photomacrography.net/forum/viewtopic.php?p=237160#237160 We all think that Kohler light from both flash and lamp is important for DIC. But Anne (above) uses a simple beam splitter cube directly under her condenser for flash (quite a bit above field iris). She got beautiful DIC images. I w...

Saying " as is...no returns." means nothing. If you haven't disclosed a fault, you have to take the return. There is no such thing as an "as is" listing. Yes I've tested it, and won. Ebay rules, rule. I agree and have the same experience. If seller says item is "used" (meaning fully functional), th...

Nice images, Kurt!

That micromanipulator looks less complex than the ones we looked at before, but looks like it is working well for you!

I don't think a complex fluidic manipulator is necessary, unless you really want to compete with people like Klaus Kemp, Michel or Beats.