Search found 187 matches

by Oliver
Sun Nov 16, 2014 11:02 pm
Forum: Identification help
Topic: Help ID please
Replies: 2
Views: 1983

Re: Help ID please

The "internal bacteria" could also be cell organelles that are moving. I can not say anything about the type of cell, the 2-part nucleus is interesting. Certain white blood cells have lobed nuclei, but I do not know if this one belongs to that category.
Oliver
by Oliver
Sat Nov 15, 2014 7:08 am
Forum: Beginner's corner
Topic: Some questions regarding special features
Replies: 17
Views: 6194

Re: Some questions regarding special features

One aspect that has not been mentioned in this thread is that Koehler illumination also allows you to limit the illuminated area on the specimen to the area which is actually observed. this is done by closing the field diaphragm. There are two advantages: a. this reduces the heting of the specimen (...
by Oliver
Fri Nov 14, 2014 6:07 am
Forum: Introduce yourself
Topic: The QCC from Kitchener, ON Canada
Replies: 7
Views: 8096

Re: The QCC from Kitchener, ON Canada

Halogen bulbs last a very long time, provided that you turn the microscope on slowly. Mine has been working since 1998. Halogen bulbs can always be exchanged by the user, there is a cover on the base of the microscope. see link below (spanish version). LED maybe not, because the contacts might be so...
by Oliver
Tue Nov 11, 2014 6:12 am
Forum: Pictures and Videos
Topic: Gneiss, a metamorphic rock.
Replies: 7
Views: 2101

Re: Gneiss, a metamorphic rock.

given that the top and bottom pictures of Gneiss look (at least to my uneducated eyes) very different, what is the common characteristic that says "Gneiss"? I guess this has to do with the direction of the cut/polish. The top one might be parallel to the layers/crystals, the bottom images perpendic...
by Oliver
Sat Nov 08, 2014 10:27 pm
Forum: Pictures and Videos
Topic: Amoeba (video)
Replies: 6
Views: 2309

Re: Amoeba (video)

Try to include the video like this:
[vimeo]111306337[/vimeo]

(this post will be removed)
by Oliver
Sat Nov 08, 2014 10:26 pm
Forum: Pictures and Videos
Topic: Cell Walls
Replies: 3
Views: 1449

Re: Cell Walls

Hello,
The holes between the "segements" could be Plasmodesmata:
http://theplentyplant.blogspot.co.at/20 ... smata.html
These are the places where adjacent cells join for cell to cell communication. There are breaks in the cell wall.
Oliver.
by Oliver
Fri Nov 07, 2014 10:44 pm
Forum: Introduce yourself
Topic: Lymia (français and English)
Replies: 13
Views: 5626

Re: Lymia (français and English)

Hello "Lymia", Welcome to the forum! For observing bacteria you need a microscope with a total magnification of at least about 400x (40x objective and 10x eyepiece). A microscope with a 100x oil immersion objective will give you 1000x maximum magnification. A total magnification of 2000x, as you wro...
by Oliver
Fri Nov 07, 2014 12:29 pm
Forum: Camera systems and imaging
Topic: Very poor image with Omax camera
Replies: 7
Views: 8229

Re: Very poor image with Omax camera

Hello, Halogen will produce a more blue light at high intensities, the two images can therfore only be compared if taken at the same intensity. The left image shows both blue background and red specimen, I therefore think that the camera is able to show the full color range. If one of the color chan...
by Oliver
Tue Nov 04, 2014 7:51 pm
Forum: Pictures and Videos
Topic: Closterium sp.
Replies: 8
Views: 2623

Re: Closterium sp.

Seb,
What lighting did you use? Did you use UV light and a dark-field filter? I tried it with blue light LEDs but without much success. The wavelength was, evidently, not correct.
Thanks,
Oliver.
by Oliver
Tue Nov 04, 2014 6:10 am
Forum: Microscopes and optics
Topic: New filters
Replies: 6
Views: 3560

Re: New filters

Hi,Oliver,may I ask what's the material you used to make filters? I used a color laser printer and printed the filters on heat-proof transparency foil (for laser printer). Transparency foil for ink jet printers ae probably not heat resistet enough. The filters do get hot when used. I have halogen l...
by Oliver
Sun Nov 02, 2014 4:12 pm
Forum: Beginner's corner
Topic: What is the Illuminating Wavelength
Replies: 3
Views: 2377

Re: What is the Illuminating Wavelength

You got the fraction the other way around.

Resolution = L / (2*NA)
= 600nm / (2*0.65) = 0.46micrometers

compare this to the values in Table 2, here:
http://zeiss-campus.magnet.fsu.edu/arti ... ution.html

Oliver.
by Oliver
Sun Nov 02, 2014 7:21 am
Forum: About the website, the magazine and this forum
Topic: Late magazine release
Replies: 0
Views: 3201

Late magazine release

I am still working on an article for the magazine, this is the reason why the pdf is a bit late this month.
Oliver
by Oliver
Sun Nov 02, 2014 7:14 am
Forum: Home-made microscope adaptations
Topic: Filter Template
Replies: 4
Views: 5178

Re: Filter Template

And also a thank you to 75RR for making the filter template available!
by Oliver
Sun Nov 02, 2014 7:13 am
Forum: Beginner's corner
Topic: What is the Illuminating Wavelength
Replies: 3
Views: 2377

Re: What is the Illuminating Wavelength

Hello, The illumination wavelength is the wavelwngth of the light used to illuminate the specimen. It is the wavelength of the microscope lamp. You need to know this wavelength in order to calculate the theoretically maximum resolution. The shorter the wavelength, the higher the resolution. Now, the...
by Oliver
Thu Oct 30, 2014 10:09 am
Forum: Pictures and Videos
Topic: Milk- Globules of fat
Replies: 6
Views: 1768

Re: Milk- Globules of fat

A bit of milk added to paramecia (and other ciliates) makes the water movement visible.
Oliver.
by Oliver
Wed Oct 29, 2014 5:22 am
Forum: Pictures and Videos
Topic: Crystals of sulphur
Replies: 4
Views: 1574

Re: Crystals of sulphur

put slide on a plate, horizontally and light the match right above it. The head of the match should be close to the slide and the flame should almost touch the slide. You can see that it forms a deposit on the glass slide. So you do not hold an already burning match to the slide, rather you burn the...
by Oliver
Tue Oct 28, 2014 9:27 pm
Forum: Pictures and Videos
Topic: Crystals of sulphur
Replies: 4
Views: 1574

Re: Crystals of sulphur

Yes, it works! There seem to be two different types of crystals. Here are some under polarized light: http://www.microbehunter.com/pictures/2014/match-crystals-1.jpg And here in bright field (These crystals were not visible when placed between crossed polarization filters): http://www.microbehunter....
by Oliver
Tue Oct 28, 2014 8:10 pm
Forum: Beginner's corner
Topic: Sample Jar Contents
Replies: 10
Views: 3977

Re: Sample Jar Contents

Feel bad about the rotifers and euglenas though. Yes, I understand what you mean, I sometimes think the same. But then again the concept of individuality maybe does not apply to these organisms. Everytime when I scratch my skin, I kill thousands of skin cells (and do not feel sorry for them). Maybe...
by Oliver
Tue Oct 28, 2014 5:46 am
Forum: Microscopes and optics
Topic: Does anyone know something about Rheinberg illumination?
Replies: 2
Views: 1937

Re: Does anyone know something about Rheinberg illumination?

I wrote about Rheinberg filters in this issue: http://www.microbehunter.com/microbehunter-magazine-august-2012/ Make sure that the central patch stop is sufficiently dark. Otherwise there is too much light passing through and the specimen itself will be too bright, taking on the color of the patch s...
by Oliver
Mon Oct 27, 2014 6:19 am
Forum: Beginner's corner
Topic: First newbie question for all you pros :D
Replies: 1
Views: 1868

Re: First newbie question for all you pros :D

Hello, I have only done fluorescence work once, long time ago, back at university, and my recommendations are based on darkfield, where you also have bright speimens on a dark background. The problem with automatic exposure is that the camera determines an average brightness of the image to set the ...
by Oliver
Sun Oct 26, 2014 12:39 pm
Forum: Microscopes and optics
Topic: New to me Zeiss Microscope. Would like some help.
Replies: 39
Views: 10918

Re: New to me Zeiss Microscope. Would like some help.

I think that the Koehler illumination has to be managed by the optics beneath the stage. This is only about the lamp. The light is directed from the back of the microscope into the base of the microscope, from where it is redirected (prism or mirror) upwards towards the stage. The question is, if th...
by Oliver
Sun Oct 26, 2014 7:27 am
Forum: Home-made microscope adaptations
Topic: dslr nikon 3100 to zeiss opmi nc 1 surgical microscope
Replies: 3
Views: 2494

Re: dslr nikon 3100 to zeiss opmi nc 1 surgical microscope

There are specific DSLR microscope adapters available:
http://www.martinmicroscope.com/product ... nts-zeiss/

But I do not know the specific requirements of your system.
Oliver
by Oliver
Sun Oct 26, 2014 7:07 am
Forum: Microscopes and optics
Topic: New to me Zeiss Microscope. Would like some help.
Replies: 39
Views: 10918

Re: New to me Zeiss Microscope. Would like some help.

First, it should not be a problem to get spare parts for a Zeiss. Second, did you consider converting the microscope to LED? Some people have done this already.
Oliver.
by Oliver
Sat Oct 25, 2014 11:08 pm
Forum: Home-made microscope adaptations
Topic: dslr nikon 3100 to zeiss opmi nc 1 surgical microscope
Replies: 3
Views: 2494

Re: dslr nikon 3100 to zeiss opmi nc 1 surgical microscope

Hello,
Please upload the jpg file directly, not the zip.
What is the cable for? This does not look like an SLR adapter. The numbers look like f-stops on a camera, however.
Oliver.
by Oliver
Sat Oct 25, 2014 11:01 pm
Forum: Camera systems and imaging
Topic: Connecting a DSLR camera to a microscope
Replies: 11
Views: 12968

Connecting a DSLR camera to a microscope

I would like to invite all forum members to share their methods of connecting a DSLR to the microscope. What adapter are you using? What projection ocular? Any advice or tips? There seems to be much uncertainty about connecting a Digital Single Lens Reflex (DSLR) camera to a microscope. The advanta...
by Oliver
Sat Oct 25, 2014 7:54 am
Forum: Specimens, samples and slides
Topic: Water organisms in cold water
Replies: 6
Views: 4824

Re: Water organisms in cold water

Hello, Organisms frequently become less active in colder temperature, because chemical reactions generally slow down the colder the temperature. Life, after all, is based on complex chemical reactions. At colder temperature, therefore, cell division is also generally slows down. I say generally, bec...
by Oliver
Sat Oct 25, 2014 5:55 am
Forum: Pictures and Videos
Topic: Clover Leaf
Replies: 3
Views: 1846

Re: Clover Leaf

The guard cells of the stomates evidently store starch. They stain much darker (by the iodine) than the other cells.
Oliver,
by Oliver
Fri Oct 24, 2014 9:46 pm
Forum: Introduce yourself
Topic: New Guy to Site (And really like it!)
Replies: 1
Views: 2029

Re: New Guy to Site (And really like it!)

There seem to be several people around who are interested in both astronomy and microscopy (myself included).
by Oliver
Fri Oct 24, 2014 7:18 pm
Forum: My microscope
Topic: Zeiss Standard 16, Reichert Biovar and Stereo a chinese
Replies: 17
Views: 7784

Re: Zeiss Standard 16, Reichert Biovar and Stereo a chinese

Manfred,
On your Zeiss I can read the name "R. Drews" written with a pen. Did you buy the microscope from Rudolf Drews, the author of the book "Mikroskopieren als Hobby" (Falken Verlag, 1992)? If it is him, did you happen to know him personally?
Oliver