MicrobeHunter.com Microscopy Forum

Hello 75RR, Remembered to measure the distance from the top of the eyepiece to the camera sensor. It is 52mm I personally do not have any experience with lifting up the ocular for direct projection to the camera sensor. I only have read the following advise (tried to translate from german): "(Optic...

Many thanks tgss, Hobbyst46 and janvangastel

It depends on which version of the SM-LUX you have.

Here is the manual. Have a look at page 3

http://science-info.net/docs/leitz/Leit ... ctions.pdf

Ive tried to read threads about darkfield on here but its gone over my head. ... what exactly do i need to add to it to get darkfield? When i google leitz darkfield i see about twenty million different 'darkfield condesers'. Just add a 'stop' under the condenser See links: http://www.microscopy-uk....

I don't know this specific microscope, but it is typical to have two adjustable eyepiece sockets in binocular tubes where the tube length changes with the interpupillary distance. You first set the ipd, then read the set value, then transfer it to the eyepiece sockets. A Siedentopf bino tube design...

Zeiss Planapo 63x/1.4, DIC, 20µm width of the diatom on the far right, stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Same session as the Grammatophora sp. posted earlier today.

Just counted the diatoms again and I think they may now be 10, will keep the title anyway.
.

Actually I wonder what is the camera length / distance with your photo adaption? With a 125mm distance the magnification factor of a 10x Objective will be 5x. So still a lot of the intermediate image will be cropped on a APS-C DSLR chip? Remembered to measure the distance from the top of the eyepie...

Probably already widely known ... but this seems a good place to quote Zeiss: . As is the general practice today, the magnification of the eyepieces is indicated by a figure followed by " x ". Letters before the magnification mark the type of eyepiece. Since we manufacture only compensating eyepiec...

. Zeiss Planapo 63x/1.4, DIC, 70µm length, stacked and stitched in Photoshop, Marine sample, Alboran Sea. Came across this empty frustule which was unfortunately somewhat mucky, still being empty allowed a little more detail of the stria and pores to be captured. Converted to grey scale. This is usi...

This is a Ningbo Haishu Honyu Opto-Electro Co. Ltd. CPD.03.30ET. They sell in China as a Honyu CPD.03.30ET. Slightly modified versions of the stand show up as many brands , including Leica, maybe the Primostar too, although it might be Jiangsu Victor.They also make the Nikon 100 and 200. The head h...

... but they were calculating the applied loads, in Newtons per [area], so the instantaneous load which initiates the cracking should start very high, and rapidly reduce as the contact area increases. Not worth worrying about it though ... it was just a thought. Mine was just a suggestion ... I won...

MichaelG. wrote: ↑

Mon Jul 13, 2020 8:13 pm

A hard glass rod, contacting a complex shape made from silica would, I suggest, only make a few ‘point contacts’ [theoretically, only three] until such time as significant distortion has taken place.

Given that this was a destruction test, I would say that significant distortion had taken place.

LouiseScot wrote: ↑

Mon Jul 13, 2020 6:05 pm

I'll double check with them tomorrow

If you can ask for a photo that shows the Oxion trinocular head with the prism lever.

. The letter to ‘Nature’ is very interesting, and the FE modelling of Diatoms most impressive ... but I was surprised by one stated assumption [near the top of the second column] regarding the testing. . Assuming that the glass needle pressed on an area of 100 [square micrometres] ... . note: I hav...

The Oxion, they say, is 80:20 as I mentioned above. I suppose, with a camera, you can just do a longer exposure. The Motic is 50:50. I do not think there is such a thing as a 80:20 on its own. When you have it (by the way it is 80 camera and 20 eyepieces) you also have the option 100 eyepieces and ...

The only one that does is part of the dual viewing head (see linked image below) not sure if you can request it or if it will even fit.

. Looking at the oxion images from their site (see linked images below) I can not see a lever that would activate a split, which leaves us with a 50/50 https://www.euromex.com/en/products/products/upright-microscopes/university-upright-microscopes/oxion/ https://www.euromex.com/en/uploads/producten/...

Ideally what you want is Candela output for each illumination type. My 12v 60w illuminator reaches 6000 Candela at 12v, but at that intensity they only last 100 hours so I run max at 10v which brings it down to 3400 candela at which rate it lasts for hundreds of hours. I have a 10w LED that reaches...

Ideally what you want is Candela output for each illumination type. My 12v 60w illuminator reaches 6000 Candela at 12v, but at that intensity they only last 100 hours so I run max at 10v which brings it down to 3400 candela at which rate it lasts for hundreds of hours. I have a 10w LED that reaches ...

A recent topic on illumination had me looking for a Zeiss 60W bulb, Zeiss number 380018 2520

Found this, thought some Zeiss owners might find it interesting

https://www.medlamps.co.uk/zeiss-type-3 ... -lamp.html

Yeah, phase contrast, darkfield and polarization are still on the cards but maybe as accessory upgrades

Do look carefully into the cost of adding it. It needs to be affordable - potential future lottery wins do not count. ;)

Lol, 60W is a lot of watts - via a mirror?

External lamp house
.

LouiseScot wrote: ↑

Mon Jul 13, 2020 12:37 pm

I suppose it depends on the illumination wattage - can I ask what yours was (if you can remember!)

A 60W incandescent light bulb.

By the way the microscope you linked to on your trinocular split query does not have Phase, is that still on the cards?

I used a 50/50 trinocular split for quite a long time and found it worked very well.

Liked being able to see through the eyepieces while photographing.

I would say that unless you are going to use very light intensive techniques such as high magnification Darkfield you will be fine.

Here's a graph of a glass filter I have. But it's one bought off of ebay and not a microscope company. So I'd like to check: Did you make the graph and if so how? This was done with an Ocean Spectrometer. A baseline spectrum was built up with a RGB LED, two UV LEDs, and one IR LED. Snapshots were s...

"Petered out" ?? means it is not open for reading because of its age ? I was referring to the fact that there are only 5 posts on the site, all dating from 2014. The Nature 2003 article nevertheless is apparently of interest, has been cited 78 times in journals. There is a later publication " Size ...

Hobbyst46 wrote: ↑

Mon Jul 13, 2020 5:29 am

"Petered out" ?? means it is not open for reading because of its age ?

I was referring to the fact that there are only 5 posts on the site, all dating from 2014.

I'd like to expand my range of magnifications via a 20x EP set. Copy pasted this from a previous post of mine - seemed simpler, this is the link if anyone is interested http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=7096&p#p62773 Useful magnification: There is a formula (well a r...

(always avoiding empty magnification - be like Ted). your be like Ted reference sent me on a wild internet search ... thought it was a Ted Nugent reference until I clicked on the link at the end of your post. :) 47 pages ... impressive this is from page 6: "In fact, some of the best microscopes ava...

.. And heard a "crack"! I know that awful feeling when one hears that sound. A couple of times it was a diatom ... and once an actual cover slip! The mechanical strength of frustules has been studied, and example: https://carabthompson.wordpress.com/2014/12/08/the-diatoms-shell/ Nice link. Does see...

Here's a graph of a glass filter I have. But it's one bought off of ebay and not a microscope company. So I'd like to check:

Did you make the graph and if so how?