Search found 7500 matches

by 75RR
Thu Jul 02, 2015 8:54 pm
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 4837

Re: Optimum Magnification Range

75RR: No, I don't think you can use an oil-immersion objective dry and expect to get acceptable images (assuming that you can get the object in focus). I thought the test was to show that one could. Wasn't it in response to the question below? Does that mean that using a 100x/1,25 objective dry wou...
by 75RR
Thu Jul 02, 2015 5:57 pm
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 4837

Re: Optimum Magnification Range

Wow! Thanks for all the work apochronaut. I have gone from assuming that one could not use an oil objective dry to having to examine the images carefully to see the difference. Went so far as to drag the two 1.25 NA Abbe images on to the desktop and then join them in Photoshop in order to make a sid...
by 75RR
Thu Jul 02, 2015 2:42 pm
Forum: Home-made microscope adaptations
Topic: Experimentations with high powered LEDs for microscope illumination
Replies: 122
Views: 24566

Re: Experimentations with high powered LEDs for microscope illumination

Update:
Resistors arrived from China in 28 days.
Dimmer presumably on its way, but since it has no tracking number, I can't tell.

Smaller than I imagined.
Caution: Batteries not included!

Image
by 75RR
Thu Jul 02, 2015 3:24 am
Forum: Microscopy accessories
Topic: Zeiss Condenser Wheel Bearing
Replies: 4
Views: 2599

Re: Zeiss Condenser Wheel Bearing

All done. Thank you very much.

Now have a working Achromatic - aplanatic phase interference contrast condenser :) :)
by 75RR
Wed Jul 01, 2015 9:51 pm
Forum: Pictures and Videos
Topic: Mayorella sp. (Amoeba)
Replies: 8
Views: 1461

Re: Mayorella sp. (Amoeba)

Thanks Rodney and billbillt
by 75RR
Wed Jul 01, 2015 9:00 pm
Forum: Pictures and Videos
Topic: Mayorella sp. (Amoeba)
Replies: 8
Views: 1461

Re: Mayorella sp. (Amoeba)

Thanks SteveSteve
I also love the other critters (Daphnia or small ostracods?) which cruise past in the background.
They are ciliates, possibly Stylonychia. Slide was full of them. I find they swim past/visit at the most awkward moments.
by 75RR
Wed Jul 01, 2015 3:47 pm
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 4837

Re: Optimum Magnification Range

Thanks JimT.
I am only now beginning to get around to reading all the books and articles I have bookmarked and never got around to.
by 75RR
Wed Jul 01, 2015 3:05 pm
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 4837

Re: Optimum Magnification Range

Hi apochronaut, I don't know the Zeiss 25X NeoFluar well enough to know it's working distance ... Working Distance of 0,54 mm. You make a good point it terms of the thought that should go into choosing a particular objective for a given task. As a general rule you can knock off about .50 when no oil...
by 75RR
Wed Jul 01, 2015 2:45 pm
Forum: Camera systems and imaging
Topic: Remembering a green hue
Replies: 10
Views: 4154

Re: Remembering a green hue

Agree with gekko that not only will just about any "colour palette" do, but that the number of colours need not be very extensive. Each reference point, i.e. coloured square, is multiplied by our perception of how close or far it is to what we are observing through the microscope. At a minimum each ...
by 75RR
Wed Jul 01, 2015 12:06 pm
Forum: Pictures and Videos
Topic: Mayorella sp. (Amoeba)
Replies: 8
Views: 1461

Re: Mayorella sp. (Amoeba)

Many thanks gekko.
A lucky break finding such a colouful one and having the video turn out so well. Still a bit hit and miss - suspect that will always be the case.
by 75RR
Wed Jul 01, 2015 12:00 pm
Forum: Pictures and Videos
Topic: Arcella sp.
Replies: 2
Views: 1019

Re: Arcella sp.

Thanks gekko. ... and very helpful ciliate. Indeed, the Arcella had flipped onto its side and then just stayed like that. I suspect I was in for a long wait as it seemed to be getting nice and comfortable, ... then the ciliate showed up. I think you could say that the ciliate not only showcased the ...
by 75RR
Wed Jul 01, 2015 5:14 am
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 4837

Optimum Magnification Range

This is a quote from Fundamentals of Light Microscopy by Douglas B. Murphy "Oculars or eyepieces are needed to magnify and view the image produced by the objective. To make optimal use of the resolution afforded by the objective, an overall magnification equal to 500–1000 times the NA of the objecti...
by 75RR
Wed Jul 01, 2015 4:13 am
Forum: Pictures and Videos
Topic: Arcella sp.
Replies: 2
Views: 1019

Arcella sp.

25x, 75µm, Phase, Olympus E-p2 Included the ciliate (possibly Stylonychia sp.) collision at the beginning because it showed several views of the Arcella, the sides and the back, that should allow for a better chance at a species ID. This time the Amoeba was home, unfortunately it did not do much mor...
by 75RR
Wed Jul 01, 2015 2:54 am
Forum: Pictures and Videos
Topic: Mayorella sp. (Amoeba)
Replies: 8
Views: 1461

Mayorella sp. (Amoeba)

25x, Phase + Oblique, Olympus E-p2

by 75RR
Wed Jul 01, 2015 1:18 am
Forum: Introduce yourself
Topic: Greetings from Belgium (part 2)
Replies: 10
Views: 3169

Re: Greetings from Belgium (part 2)

Hi Lichen09, welcome.
I'm currently a big fan of the brand 'Euromex = dutch microscope brand'
Big fan as in you have one or are you thinking of getting one?
Do post images of your microscope in the My microscope section.
by 75RR
Tue Jun 30, 2015 4:32 pm
Forum: Pictures and Videos
Topic: Centropyxis sp.
Replies: 7
Views: 1902

Re: Centropyxis sp.

Many thanks Crater Eddie, JimT and Naphthalene. I believe it would be nice to include a scale bar! I do mention the size in the description. Perhaps it might be helpful to include one, will have to figure out how to do that. I also love your copyright stamp Thanks, it was fun designing it. It is a p...
by 75RR
Tue Jun 30, 2015 11:41 am
Forum: Pictures and Videos
Topic: Spirostomum sp
Replies: 10
Views: 2388

Re: Spirostomum sp

Thanks Crater Eddie. That is very kind of you to say so.
by 75RR
Tue Jun 30, 2015 11:27 am
Forum: Pictures and Videos
Topic: Centropyxis sp.
Replies: 7
Views: 1902

Re: Centropyxis sp.

16x*1.25, 250µm, Oblique, 15 image stack in Photoshop
Last of the set from that slide, this one shows slightly different building material.

Image
by 75RR
Tue Jun 30, 2015 10:58 am
Forum: Pictures and Videos
Topic: Centropyxis sp.
Replies: 7
Views: 1902

Re: Centropyxis sp.

16x, 250µm, Oblique, 14 image stack in Photoshop This is from the same slide, taken with the 16x as it was a little too large to use the 25x There were quite a lot of Centropyxis about in the sample - no amoeba were visible however. http://i1368.photobucket.com/albums/ag171/mapologo/Centropyxis250mi...
by 75RR
Tue Jun 30, 2015 1:36 am
Forum: Pictures and Videos
Topic: Halteria
Replies: 4
Views: 1251

Re: Halteria

Good capture. I can't as yet get a sharp image even with the slow ones.
Thanks for giving the camera details.
by 75RR
Mon Jun 29, 2015 10:07 pm
Forum: My microscope
Topic: My new trinocular machine
Replies: 16
Views: 5220

Re: My new trinocular machine

Looks brand new! Congratulations
A 5 objective turret is a very nice thing to have. (It is on my wish list)
You mention 4x, 10x, 40x and 100x Plan Achromats, is the fifth a 20x?
by 75RR
Mon Jun 29, 2015 2:47 pm
Forum: Pictures and Videos
Topic: Spirostomum sp
Replies: 10
Views: 2388

Re: Spirostomum sp

Many thanks mrsonchus and JimT

Seeing the internal flow was almost hypnotic.

First one I had ever seen or heard of for that matter.
It was a case of The Ciliate Diversity Chart to the rescue. :)
by 75RR
Mon Jun 29, 2015 1:52 pm
Forum: Home-made microscope adaptations
Topic: A condenser quest.
Replies: 38
Views: 16640

Re: A condenser quest.

So, I set out to make an estimation of the way I wanted this condenser to work and assembled a prototype from lenses I had on hand. Here's the first try.
Can you give us an idea of what you mean by this? What was the theory behind this?
by 75RR
Mon Jun 29, 2015 1:41 pm
Forum: Camera systems and imaging
Topic: centre white spot
Replies: 3
Views: 2433

Re: centre white spot

... the image through the microscope eyepiece is fine).
Does that include the image through the trinocular eyepiece?

See links:
viewtopic.php?f=15&t=882
by 75RR
Mon Jun 29, 2015 2:25 am
Forum: Pictures and Videos
Topic: Spirostomum sp
Replies: 10
Views: 2388

Re: Spirostomum sp

Thanks Dennis. There are many areas within Microscopy, this is mine.
by 75RR
Mon Jun 29, 2015 12:57 am
Forum: Pictures and Videos
Topic: Spirostomum sp
Replies: 10
Views: 2388

Spirostomum sp

25x*1.25, Phase + Oblique, Olympus E-p2

by 75RR
Sun Jun 28, 2015 11:28 pm
Forum: Pictures and Videos
Topic: Cyclidium sp.
Replies: 1
Views: 1158

Cyclidium sp.

25x*2, 35µm, Phase + Oblique, Olympus E-p2

by 75RR
Sun Jun 28, 2015 11:21 pm
Forum: Pictures and Videos
Topic: Peranema II
Replies: 7
Views: 1683

Re: Peranema II

Thanks Naphthalene
Peranema are fun to watch - talk about becoming what you eat!
by 75RR
Sun Jun 28, 2015 4:47 pm
Forum: Camera systems and imaging
Topic: Remembering a green hue
Replies: 10
Views: 4154

Re: Remembering a green hue

Thanks The QCC,
link in fact is the first one in the opening post.
I no longer have a printer - I do not print much and when I do I find it much cheaper and simpler to use a local print-shop.
by 75RR
Sat Jun 27, 2015 11:34 pm
Forum: Pictures and Videos
Topic: Centropyxis sp.
Replies: 7
Views: 1902

Centropyxis sp.

25x, 190µm, Oblique, 13 image stack Photoshop
Managed to get some detail on the Diatom at the bottom of the aperture as well, which was nice.

Image