MicrobeHunter.com Microscopy Forum

My only (minor) complaint is that the illumination could be a little brighter since I am now splitting the light three ways.

If you are not able to move the prisms, then the light is most probably 50% for the photo tube and 25% each for the eyepieces. Essentially a 50/50 split.

Congratulations on the Olympus BH-2.

link to another owner who you may want to pick up some tips from:

http://www.krebsmicro.com/microsetup2/

Very nice. Such delicate movement. A nice change from the ungainly actions we sometimes see.

I once downloaded from the web a Zeiss document explaining succinctly how to use the Michel-Levy chart, but later discovered that one page was missing. As that particular brochure is available from the Zeiss Home site (http://www.zeiss.com/microscopy/en_de/downloads/brochure-downloads.html?catalog=...

Nice image. What camera settings did you use to freeze it?
Good to see the Trinocular in use!

Many thanks gekko, very kind. Have amended the topic to read Pine Spore Germination! Sorry for the oversight.

Many thanks Charles and Crater Eddie.
Have been taking a second look at spores thanks to the slide mailer.

25x *1.25, 70µm, Oblique and Pol, 3, 4, 3 and 4 image stacks in photoshop This is the same spore as before (http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=2073) - just a few hours later and then the next morning. http://i1368.photobucket.com/albums/ag171/mapologo/A196-198ww15_zpsb...

Not native. But I believe pine trees were planted during the fifties and sixties in the Mountain/National Park that borders the city. There are also ornamental ones in peoples gardens. Do not know which is the guilty party.

I tend to close the condenser diaphragm just enough to see an increase in contrast, then I use oblique to increase the contrast further. Objective is a Zeiss Neofluar 25x/0,60 Ph 2

Thanks gekko

What happens if you only take a single image where the "edges" of the top filament is in focus?

Image 9/27

Thanks gekko,
Interesting link, certainly looks like a pine spore. Not sure where it would have come from. Will have a look around.

Wonderful image.

Thanks charlie. The last one is my favorite.

Thanks JimT. Colour as I recall was about right, a paler green than usual.

As far as I know they are the same. Mine just pulled out. Resist the impulse to hit it with a hammer. ;) And what is that screw in lens on my binocular, the one marked "C" ? Not sure what you have there, mine looks like Charles' one. Is there a way to rotate it from outside? Do you have a larger pho...

It is a strangely eerie image.

25x *1.25, 70 µm, Oblique and Pol, 5, 3 and 2 image stacks in Photoshop First image is Oblique, second one is partial Pol and the third is complete Pol (extinction) http://i1368.photobucket.com/albums/ag171/mapologo/A180-184ww_zpsnlkz7sbw.jpg http://i1368.photobucket.com/albums/ag171/mapologo/A188-1...

I must need more practice, can only see 3 in the first photo.
Wonderful last photo, like how to holds out the tentacles.
The budding looks interesting as well.
Great xmas project!

Reading further down the page it would seem that that is the price for the Plan Achromatic upgrade if you buy the microscope.

Yes that's the one. Thanks very much.

Anyone have a manual/instructions for opening a Zeiss Binocular Head - Type Jentsch
Need to clean out some cobwebs and stuff - do not really want to open it without some sort of guide.

exposure and focus using FILM???

Agree. Can't get my head around the amount of wasted film and frustration, let alone the cost. We should all drink a toast to digital.

Thanks vasselle, I had not thought of applying selective stacking and stitching in these cases. Sounds promising. Still have the slide sitting in a mailer, might have another go at it to see if skipping images really is an improvement. Unfortunately alga had moved too much. Will attempt at a later d...

Many thanks rnabholz.
Still have the slide sitting in a mailer, might have another go at it to see if skipping images really is an improvement.

Thanks for the photos. Interesting arrangement. Still not quite sure what the viewfinder adds to your setup. I was under the impression its role was to complement the Zeiss microscope cameras that lacked a viewfinder. Does it have an additional function? http://www.science-info.net/docs/zeiss/ http:...

25x, 50µm, Brightfield, 27 image stack in Photoshop The slight ghosting on the edges of the top alga is due to a jump I made in the image sequence in order to avoid merging the top and bottom algae. Now that I think about it, it might have been best to take the images and then omit them in post proc...

Your darkfield stops are working well. 10x objective?

Those look nice. What sizes did you have made up?

That is very nice. Thanks for posting.
As you say, a big step up from an old Tasco!

Just downloaded a brochure, it says:

Built-in transmitted illumination system, 0.5 W LED

Surprisingly low watts, do you happen to have any information on it?