Search found 7593 matches

by 75RR
Mon Jul 06, 2015 5:00 pm
Forum: Pictures and Videos
Topic: Alga in Brightfield/Phase + Oblique Test
Replies: 18
Views: 2807

Re: Alga in Brightfield/Phase + Oblique Test

Thanks apochronaut
I see more detail on the 40X bright phase with slight oblique.
Does bright/dark phase refer to the amount of illumination you gave it or is it a type of illumination?
A rotating stage is the next step in order to be able to alter the angle I can illuminate the subject from.
by 75RR
Mon Jul 06, 2015 4:35 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Had another look at it, used my UHL Rod Microscope. It would seem that it is on the outside of the bottom lens and it looks like the coating has been damaged. Viewed from the side it has that sheen that you get when there is oil on water. Tried to take a photo but did not manage it. What I would do...
by 75RR
Mon Jul 06, 2015 1:03 pm
Forum: Pictures and Videos
Topic: Algae
Replies: 6
Views: 996

Re: Algae

And that Zygnema is really gorgeous
Thanks gekko Lucky find.
by 75RR
Mon Jul 06, 2015 12:54 pm
Forum: Pictures and Videos
Topic: Alga in Brightfield/Phase + Oblique Test
Replies: 18
Views: 2807

Re: Alga in Brightfield/Phase + Oblique Test

Thanks gekko But I agree with Judd: I would have avoided focus stacking for the purpose of comparing the different lighting techniques-- it sort of muddies the water, I think. It does add another variable but assuming the stacks are comparable the difference in technique should still stand out. ... ...
by 75RR
Mon Jul 06, 2015 12:50 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Had another look at it, used my UHL Rod Microscope. It would seem that it is on the outside of the bottom lens and it looks like the coating has been damaged. Viewed from the side it has that sheen that you get when there is oil on water. Tried to take a photo but did not manage it.
by 75RR
Mon Jul 06, 2015 2:13 am
Forum: Pictures and Videos
Topic: Algae
Replies: 6
Views: 996

Re: Algae

Thanks JimT
Was thinking of getting a phase contrast kit for my scope but with all the various filter combinations and having seen images with and without I am not convinced it's worth it.
Not sure to what filters you are referring to. Are you referring to cost when you say "worth it"?
by 75RR
Sun Jul 05, 2015 11:07 pm
Forum: Pictures and Videos
Topic: Algae
Replies: 6
Views: 996

Algae

25x and 25x*1.25, 30µm, Brightfield + Oblique and Phase + Oblique, 8, 8, 9 and 7 Image stacks in Photoshop http://i1368.photobucket.com/albums/ag171/mapologo/Alga86-93_zpsrxxtt6u3.jpg http://i1368.photobucket.com/albums/ag171/mapologo/Alga127-134_zpsvnwzxioc.jpg http://i1368.photobucket.com/albums/a...
by 75RR
Sun Jul 05, 2015 11:00 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Thanks apochronaut,
Agree it does not look like delamination so thought dry oil sounded like a good candidate.
"scratching off the coating in the centre area of the lens" sounds a lot worse.
I will have a careful look in the morning and see if I can provide more details.
by 75RR
Sun Jul 05, 2015 8:18 pm
Forum: My microscope
Topic: My new trinocular machine
Replies: 16
Views: 5382

Re: My new trinocular machine

by 75RR
Sun Jul 05, 2015 8:10 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Inside, covers about 3/4 of the bottom lens. You need to view it at an angle to see it.
by 75RR
Sun Jul 05, 2015 4:54 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Hi apochronaut, this is the objective (same one from ebay) http://www.ebay.com/itm/Carl-Zeiss-Plan-40x-0-65-160-0-17-Microscope-Objective-46-07-10-9904-/271916774346?pt=LH_DefaultDomain_0&hash=item3f4f80b3ca Image taken though Optovar - Think it is on bottom lens. http://i1368.photobucket.com/albums...
by 75RR
Sun Jul 05, 2015 1:43 pm
Forum: Pictures and Videos
Topic: Alga in Brightfield/Phase + Oblique Test
Replies: 18
Views: 2807

Re: Alga in Brightfield/Phase + Oblique Test

Double post
by 75RR
Sun Jul 05, 2015 1:40 pm
Forum: Pictures and Videos
Topic: Alga in Brightfield/Phase + Oblique Test
Replies: 18
Views: 2807

Re: Alga in Brightfield/Phase + Oblique Test

Thanks billbillt.

I find Oblique quite effective, just showing views with (right) and without (left).
by 75RR
Sun Jul 05, 2015 10:33 am
Forum: Resources (online, books etc.)
Topic: Photomicrography - Mounting a Camera - Afocal - Projection - Parfocality
Replies: 3
Views: 4154

Re: Photomicrography - Mounting a Camera - Afocal - Projection - Parfocality

Looking at the photo you provided with the caliper http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=9&t=1192, I am not sure if that would be 40mm or 38mm, it does need a little wriggle room. Here is a link: http://www.martinmicroscope.com/product-category/digital-camera-adapters/c-mount...
by 75RR
Sun Jul 05, 2015 2:59 am
Forum: Pictures and Videos
Topic: Alga in Brightfield/Phase + Oblique Test
Replies: 18
Views: 2807

Alga in Brightfield/Phase + Oblique Test

25x, 40µm, Brightfield, Brightfield+Oblique and Phase, Phase+Oblique 8, 6, 6, and 6 image stacks in Photoshop The idea was to use the same subject in order to provide a valid comparison of the Oblique effect. Set the White Balance in Phase but did not increase illumination which is why the backgroun...
by 75RR
Sat Jul 04, 2015 9:58 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

It is on the bottom lens.
Can a 40x be opened and cleaned?
by 75RR
Sat Jul 04, 2015 9:52 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

75RR wrote:
The last two shots do show quite a difference. Maybe your Plan 40 needs cleaning?
Will clean more carefully this time.
Had a careful look - delamination. Damn!
by 75RR
Sat Jul 04, 2015 9:26 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

I think Dennis saw the Olympus E-p2 camera in your sig block and probably thought it was a microscope.
That could be it.
The last two shots do show quite a difference. Maybe your Plan 40 needs cleaning?
Will clean more carefully this time.
by 75RR
Sat Jul 04, 2015 7:40 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Why do you have???-
It is called a signature
➔ User Control Panel ➔ Profile ➔ Signature
It is just a list of my equipment.
Anyhow it has been bought and is on the way. It is exclusively for Phase Contrast use.
Good luck.
by 75RR
Sat Jul 04, 2015 7:02 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

It looks good in pics (Olympus microscope) , a sticker says serviced in 2007, and comes in original wood box. (good sign also) Make sure you do some research on that type of microscope first (do you need it? why? how does it compare with other models, average price, price of parts you may want to g...
by 75RR
Sat Jul 04, 2015 6:20 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

BUT....... when I look inside the eyepiece instead of using the USB camera it looks great. That might be down to the camera. BTW I bought a Olympus BH BHC Phase Contrast microscope for a little over 3 bills and I'll let you know if I need help on that! Looks like a really great microscope!!! I have...
by 75RR
Sat Jul 04, 2015 5:31 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

Unprocessed F and Plan images, complete area:

Image
Image
by 75RR
Sat Jul 04, 2015 5:17 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

To my eye, I can see a little more in focus or sharpness at the edge with the plan objective... The second enlarged image (F) looks sharper to me. At that distance from the center of the image I do not think we should be looking for edge improvements. All four objectives should cover that area easi...
by 75RR
Sat Jul 04, 2015 4:32 pm
Forum: Camera systems and imaging
Topic: How to mount DSLR over infinity objectives (trinocular)?
Replies: 4
Views: 3642

Re: How to mount DSLR over infinity objectives (trinocular)?

These links should help. Second link is the manual in French. On page 8 is says it is a C mount. http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=15&t=882 https://www.google.co.ve/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0CCwQFjAC&url=https%3A%2F%2Fwww.loisirsplaisirs...
by 75RR
Sat Jul 04, 2015 4:22 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

It does appear to be slightly more in focus at the edge... Just slightly... Maybe Plan objectives are not worth the extra money for hobby use.... This image covers a small central area of the field of view, the Plan "advantage" should not visible. Think concentric circles, a normal objective would ...
by 75RR
Sat Jul 04, 2015 3:45 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

Re: 40x Objectives Comparison

I don't see it as really "bad", I just don't see where it does much....
You must not expect it to have more depth of field, I believe Plans just extend the area in focus nearer to the edges of the field of view.
I am looking for more resolution/sharpness and a reason is washed out.
by 75RR
Sat Jul 04, 2015 3:36 pm
Forum: Microscopes and optics
Topic: 40x Objectives Comparison
Replies: 40
Views: 7083

40x Objectives Comparison

Decided to compare my 40x Objectives, mostly because I was a little disappointed with my Plan 40x. Not only does it seem a bit washed out but it also consequently requires closing down the diaphragm that much more. Even then it does not impress much; yet I can not see any delamination to account for...
by 75RR
Sat Jul 04, 2015 10:09 am
Forum: Pictures and Videos
Topic: Mystery Worm movie
Replies: 4
Views: 959

Re: Mystery Worm movie

I wonder if the worm is injured middle of body.
Agree with IanW - that is what it looks like.
by 75RR
Fri Jul 03, 2015 2:34 am
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 5088

Re: Optimum Magnification Range

Nice closeup shot of a nice looking diatom.
I need to revisit this and take some comparison photos.
Comparison photos would indeed be interesting. Like the comment about the oily mess!
by 75RR
Fri Jul 03, 2015 12:39 am
Forum: Microscopes and optics
Topic: Optimum Magnification Range
Replies: 23
Views: 5088

Re: Optimum Magnification Range

Well, I guess I could tell you the answer... the 100X objective will make an image, but it is not worth the extra magnification at the expense of everything else. A 60X dry objective would be a much better solution. This is an opinion based upon empirical evidence. lorex Many thanks. I had been thi...