MicrobeHunter.com Microscopy Forum

Some useful links:
http://www.ucmp.berkeley.edu/chromista/ ... phyta.html
http://westerndiatoms.colorado.edu/abou ... re_diatoms
http://academics.smcvt.edu/dfacey/Aquat ... atoms.html (An Article)
http://www.diatoms.co.uk/ad/index.htm

Hi Lymia, I am afraid I am going to have to insist on my suggestion that you contact a Laboratory Technician if you are determined to continue with your plan to identify the bacteria that causes Lyme’s disease with a microscope. At the very least you will require training, training that is neither p...

I like the translucence you get in Darkfield.

The names seem quite apt. I must say that your lighting of the subjects has improved.

If I may, I will suggest a couple of things. Firstly, since you are determined to test for Lyme disease and the cautions that both Oliver and gekko mention are very valid, you might consider contacting a Laboratory technician who would be willing, say on a weekend, to either do the tests or train yo...

Very nice. Like the way you managed to keep the glass-like edges.

Amoeba are always interesting and difficult to film.
Fastest internal movement I have seen so far.

Well done 75RR, I always admire your perfect clean background, Photoshop? yes I use one of three techniques or a combination of them in Photoshop, depending on which I think will be easier. 1.) I make a duplicate background layer (by highlighting the background layer in the layers palette and click...

... I am going to learn all by myself with my microscope... and maybe with the help of people having microscopes on this forum :) . Hi Lymia, I am just a beginner, bought my microscope second hand in April this year, but I would suggest, if your budget will allow it, purchasing a Trinocular microsc...

25x, about 100µm, Offset Darkfield Stop, 4 image stack in photoshop

Thanks for the alternate image. It provides a very good illumination comparison. I use it with 10w LED or 60w 12V bulb. Am trying to convert my Zeiss 60w Illuminator to led at the moment (forced conversion - it is missing some parts and not working), can you provide details: photos/drawings etc... +...

Very nice. Particularly like the achro 10x abdomen shots and the Darkfield. Going to have to try glycerine mounting.

Very nice. Have never tried to mount in glycerine, it is quite effective.

Very very nice!

Thanks for the id.
Looked up "plumatellid" they are very beautiful.
Found this page, if you scroll down there is an image that could be mine :)
http://university.uog.edu/botany/474/fw/plumatella.htm

25x, about 400µm, Brightfield and GUF, 16 and 13 image stack in photoshop The Oedogonium germling is the small alga on the left. http://i1368.photobucket.com/albums/ag171/mapologo/x2Oedogonium-germling400microm_zps2e66d715.jpg http://i1368.photobucket.com/albums/ag171/mapologo/statoblast400microma_z...

Amscope obj. (I have an Amscope microscope). Achromatic and NA is 0.85 bought on Amazon. Here is the link:

Many thanks

75RR, what specific info would you like to know?

I suppose like most of us I would like to get a dry 60x someday, so the make + NA perhaps a link to where you got it if it is new. The usual suspects.

Hi JimT,
can you provide some info on the 40x and 60x objectives?

Surreal image. I like it.

Question on the "micro*scope" site.
I had come across this very good site before but got the impression that it was no longer being updated.
Do you know anything about it?

Very nice.
Did you also take some photos with another form of illumination?
I find the comparison of different techniques on the same subject interesting.

Thought it was attached to something but it would seem that it is not, it is dragging a second longer flagella. Can not find any images of Chilomonas with second longer flagella. How about anisonema? Spoiler: This suggestion is based on a quick internet image search, i.e. a very wild guess! Your pot...

Both videos are of Rotifers. There is an enormous variety and yes, they do spend all their time eating.
I find them most interesting.
See link for an Idea of the variety there is.

http://microlife.parvarium.com/Gnathifera.html

Nice image + good illumination
Have you considered placing a nettle sting under your compound microscope.
Worth a try I think.

Very nice.
Agree with gekko, they are among the nicest things to find.

Making up a new set of Darkfield Stops and GUF Filters (Gradient Universal Filter) in pdf to be printed on acetate. Feel free to try them out. DF Stop Filters are 32mm diameter. Print at 100% Darkfield Stops, which go from 5mm to 16mm in 1 mm steps are in pairs so they can be glued face to face. Cut...

Must have missed that last photo. I am assuming you posted it before? Very interesting!

Indeed they do.

Can you confirm/list illumination please.

1 Darkfield
2 DIC
3 ?
4 Phase

Thanks

How did you manage to get the images for the stack? Was it not moving?

They do seem to spend most of their time twirling about, now and again they seem to rest, not sure why.