MicrobeHunter.com Microscopy Forum

Have a chat with your university's head of clinical or epidemiological microbiology. They have all the resources you will need.
If you want to do research at a university medical school, you need to go through the proper channels.

No offense, but I think you need to go back and reassess what you want to do, and why. You say you want to seen protein and collagen, but some pretty simple things like phase contrast and polarization are a bit over your head. To be brutally honest, until you can be a lot more specific about your qu...

Nicely done! I appreciate the labels, too. They're quite a bit of work, so most people don't use them.

Tom

My Olympus BHS system produces nice dark backgrounds as well as the full range of colors depending on how far the analyzer prism is translated. On the other hand, my Olympus BX system with the DICT slider will not produce a good darkfield effect with or without the 530nm waveplate. Only the full ran...

microscopeboi, For a little bit of introductory homework, see: https://en.wikipedia.org/wiki/Differential_interference_contrast_microscopy https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/dic/dicintro/ https://en.wikipedia.org/wiki/Waveplate https://www.youtube.com/watch?...

Thanks Michael!

Anything you output to a 720p system will be limited to 720. The original resolution doesn't matter. Even 8K to 720 still gives 720, and that's pretty low-res when blown up to projection scale. 720 looks pretty good on a laptop screen. If you double the size vertically and horizontally (essentially ...

I used "Weld-On 4SC".
On Amazon: https://www.amazon.com/SCIGRIP-Adhesive ... ast_sto_dp

ewieckow, As you requested, here are the two I use. The first is a custom one I built out of 1/4" ABS plastic glued together, and 1" urethane foam (I think). The foam is pretty stiff and is glued in place with 3M 77 adhesive. Any slots or holes needed, like for the lamp house, were routed out. Notic...

What woyjwjl said: From the price/performance point, the M43 camera (Panasonic GH4) + NFK3.3 + U-PMTVC adapter (catalog number U-V210, 037151) provides perfect access to 4K images and videos. That combination, along with DIC on my BHS, is what I used to shoot the 2018 Nikon Small World in Motion Hon...

Your D3200 is limited to Full HD per Nikon's website, 4K is not possible. You'll need a camera that will output 4K video to clean HDMI. Your computer screen will need to be 4K as well. That's not happening with an old laptop. Your cam-link clone is probably not 4K either. 4K takes a high-throughput ...

Don't try to carry it all the time. It's too big and too awkward. With that much weight in front of you, it's hard on your back, and stairs will be very dangerous. You will get hurt. I have seven BHTU's, two of which are trinocs, that I transport for outreach events. Six are fitted upright, in custo...

Pasteur pipettes are a little difficult to use with such small volumes. Capillary tubes are better for loading the chambers. Cheaper, too. The thicker cover slips are heavier and less prone to float on top of the specimen if it's viscous. Otherwise, either one works fine. Unless you get one with the...

Wow.

Judge, So far, this doesn't make much sense. If you hit the slide, the stop is not the problem. The stop is there to keep you from hitting the slide. This isn't always as easy as it seems to be, particularly if you're on your own with no one next to you to ask. On-line troubleshooting things like th...

Send them an email and ask.
Their email, from the website is: support@swiftoptical.com

The short answer is no. There is nothing to see. Those affect platelet function, generally not platelet size or number.

And to help the cell phone photgraphy along, here is a Micscape article I wrote on using your cell phone camera for high quality still and video photomicrography: http://www.microscopy-uk.org.uk/mag/art ... omicro.pdf

You will be amazed at how well it works.

Price?

Remember, the LWD and ULWD CDPlan PL objectives are for the inverted Olympus IMT-2 microscope. The IMT-2 uses a different condenser than the upright BH-2 so the phase rings *might* not be the same size. I've never checked. The non-phase objectives should work. I have a friend who has been using them...

The Olympus BX-60 is a very nice microscope. It's set up as a polarizing scope. Easy to do other things with; DF, BF, phase, DIC with the right condensers, etc. Good objectives (U-PlanFluorites), are not cheap, but easy to find. For perspective, last year I paid about $1350 for the U-CPA Conoscopic ...

You need the DIC slider that fits under the objective turret. The slider is on the right in the photo:

https://www.spachoptics.com/OLYMPUS-IMT ... t2-dic.htm

You have non-plan objectives. The plan ones have a flatter field so you can see more in focus at once. Most clinical lab have bought plans as the tech efficiency is a little better with them. When I first started in clinical labs 40+ years ago, lots of the scopes had non-plan objectives due to the p...

It's really hard to read in the photo, but if you look between the back of the stage and the arm, on the top of the stage mount, you will find the model designation stamped into the metal.

It appears to read FHA.

Autofluorescence. No dyes necessary. You use the intrinsic fluorescence of the organism. https://www.microscopyu.com/references/autofluorescence https://www.nikonsmallworld.com/galleries/2014-photomicrography-competition/autofluorescence-in-marine-algae https://www.nikonsmallworld.com/galleries/2008...

It has a nice "Game of Thrones" feel to it.

Like this: https://www.ebay.com/itm/133862944975?h ... SwKypgvY7n

I well understand the complaints about a microscope light source changing from halogen to LED. I'm not adverse to technological change at all, in fact I love new toys. But the change in color temperature that came with the switch (with one new Oly BX-43 LED microscope) was dramatic. It required "rec...

No. The mount for the BX is bigger than the mount for the BH-2. The BH-2 dovetail stage/condenser mount assembly doesn't fit the BX frame either. I've never tried a BH, but it's about the same size as the BH-2. I've adapted a BX stage to a BH-2 but not the other way around. You need to find a BX sta...

I understand dermatopathologists and dermatologists will sometimes use 1x-2x objectives. Remember, if they're only looking for clear margins, there is usually a fairly distinct difference in the stained cells between malignant and normal. If there are no malignant cells at the margin they quit cutti...