Search found 124 matches

by Tom Jones
Sat May 04, 2019 3:09 am
Forum: Identification help
Topic: Unknown blood cells in blood sample
Replies: 6
Views: 2230

Re: Unknown blood cells in blood sample

:roll: You should stop doing tests on yourself if you don't know what you're doing. :?

Perhaps a perusal of this might help: https://onlinelibrary.wiley.com/doi/boo ... 1444398595 8-)

On the other hand, I've heard Gwyneth Paltrow's Goop might be good for this... :twisted:
by Tom Jones
Sun Apr 14, 2019 3:47 pm
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4448

Re: Tips on Buying a Microscope and suggestions for What to look at

Very comprehensive and very nicely done! Not to be too picky, but just below your image of two older microscopes, you indicate the Unitron microscope is on the left, while it is actually shown on the right. ;)
by Tom Jones
Tue Apr 02, 2019 3:39 pm
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3830

Re: How to attach camera to Olympus BH2?

I use that very setup to project, essentially direct project as the NFK 3.3x and 0.3x lens in the U-PMTVC essentially cancel magnifications, onto the sensor of my Panasonic GH4 for 4K video. Since the GH4 is mirrorless, a C-mount adapter fits fine, and I get the necessary final corrections in the NF...
by Tom Jones
Fri Mar 08, 2019 3:52 am
Forum: Microscopes and optics
Topic: 100x NCG oil: what is it used for?
Replies: 5
Views: 1197

Re: 100x NCG oil: what is it used for?

Almost all clinical labs use standard 100x 0.17 oil immersion objectives, with oil and without cover slips, for blood smears, body fluid smears, or gram stains for that matter. Lower power objectives are used with a thin film of oil substituting for the cover slip there as well. Cover slips are cost...
by Tom Jones
Fri Feb 08, 2019 1:36 am
Forum: Pictures and Videos
Topic: Aeolosoma sp.
Replies: 11
Views: 1900

Re: Aeolosoma sp.

Nice. It's even budding!
by Tom Jones
Sat Feb 02, 2019 12:36 am
Forum: Beginner's corner
Topic: Picture magnification question?
Replies: 8
Views: 2557

Re: Picture magnification question?

One other thing to consider is to be sure the picture/video format you want to use is the same one you use to calibrate. Most cameras will use different pixel dimensions on the sensor for each available resolution and/or aspect ratio. That can rather dramatically change the number of pixels for a gi...
by Tom Jones
Mon Jan 28, 2019 5:05 am
Forum: Beginner's corner
Topic: Eye floaters
Replies: 14
Views: 4704

Re: Eye floaters

GOLGO13, Ignoring your floaters is something that is pretty much universally recommended. It's generally good advice for most people. However, if they are really debilitating, you actually have a couple of choices to get rid of them. It will be a bit of a slog to convince the average ophthalmologist...
by Tom Jones
Wed Jan 09, 2019 5:17 pm
Forum: Off Topic
Topic: This FREE 6-week course in how to design experiments in biological research..
Replies: 2
Views: 3511

Re: This FREE 6-week course in how to design experiments in biological research..

You're right Billt. That does look interesting. Thanks! For those not aware, in addition to classes like this one, iBiology has many video lectures on various aspects of biology research. They have also a very nice, and very complete, lecture series on microscopy techniques: https://www.ibiology.org...
by Tom Jones
Mon Jan 07, 2019 3:33 pm
Forum: Microscopes and optics
Topic: Olympus BH-2 Question. Lamp intensity adjuster. Faulty?
Replies: 17
Views: 4757

Re: Olympus BH-2 Question. Lamp intensity adjuster. Faulty?

Before you get too carried away, you might try squirting contact cleaner on the slider (through the slider opening) and then moving the knob back and forth the full range several times. Dirt on the slider can make contact intermittent at best. I've resurrected several this way.
by Tom Jones
Tue Jan 01, 2019 3:30 am
Forum: Beginner's corner
Topic: Recommendations for a good text book to aid and explain what I am seeing.
Replies: 5
Views: 3074

Re: Recommendations for a good text book to aid and explain what I am seeing.

My edition of Ward and Whipple is from 1959. Quite a few are available used on Amazon: https://www.amazon.com/Fresh-Water-Biology-George-Chandler-Whipple/dp/047123298X Take a look at the download 1918 version first to see if you think it will meet your needs. Don't worry about being location specifi...
by Tom Jones
Mon Nov 12, 2018 4:21 am
Forum: For forum members who want to buy and sell equipment
Topic: For Sale: Zeiss Junior
Replies: 4
Views: 1470

Re: For Sale: Zeiss Junior

wstenberg, What/who was your source for the Chinese oculars? Any idea how well they would work with Zeiss objectives? I have several ex-university Standards that all had delaminated oculars I need to try to replace. Since they'll be donated to a middle or high school after refurbishment, Zeiss ocula...
by Tom Jones
Fri Oct 19, 2018 2:31 pm
Forum: Home-made microscope adaptations
Topic: Monitor mount
Replies: 7
Views: 3931

Re: Monitor mount

Radazz,

Interesting setup. I like it.
I see you also have a copy of Shaw on your bookshelf. 8-)
I bought mine when it came out, have read it many times and learned a lot!

Tom
by Tom Jones
Mon Oct 15, 2018 2:55 pm
Forum: Beginner's corner
Topic: pls help with recc. a microscope
Replies: 19
Views: 3615

Re: pls help with recc. a microscope

Hi Bob, Remember, a bit of digital zoom isn't much of an issue when you start with a 4K sensor and are down sampling to HD for the output anyway. It is still pretty much guaranteed to be better than an eyepiece camera at 640 x 480 with their hideously slow frame transfer rates. As a cure for your MH...
by Tom Jones
Mon Oct 15, 2018 2:24 am
Forum: Beginner's corner
Topic: pls help with recc. a microscope
Replies: 19
Views: 3615

Re: pls help with recc. a microscope

Hi Bob, I'm not sure it's in all of them, but it's certainly in most of them. It's been around for quite a while. iOS has it's own method that works fine too, with iPhones and iPads. Here's a list: http://www.mhltech.org/devices.aspx and another: https://hudly.zendesk.com/hc/en-us/articles/115003409...
by Tom Jones
Sun Oct 14, 2018 4:11 am
Forum: Beginner's corner
Topic: pls help with recc. a microscope
Replies: 19
Views: 3615

Re: pls help with recc. a microscope

Lisa, Get Lorez's microscope. Get a good cell phone adapter, mount the cell phone on the microscope eyepiece (horizontally please - NO vertical video as you loose too much screen real estate and resolution), hook up an HDMI adapter from the cell phone to the HDMI input for the white board. Voila! Yo...
by Tom Jones
Sat Sep 22, 2018 3:59 am
Forum: Illumination Techniques
Topic: Better circular compass cutter
Replies: 11
Views: 2830

Re: Better circular compass cutter

I use a 3-50mm punch set from Mayhew that I like a great deal. Not cheap at all, but if you're a perfectionist, this is as good as I've seen. It will even cut concentric circles: https://www.amazon.com/Mayhew-66010-Met ... 82QRI?th=1
by Tom Jones
Fri Aug 24, 2018 4:42 pm
Forum: For forum members who want to buy and sell equipment
Topic: Glass-bottomed culture dishes for sale
Replies: 10
Views: 2497

Re: Glass-bottomed culture dishes for sale

Thanks Bill! I've been meaning to look up a source for those.
I just ordered a couple of them from you.

Tom
by Tom Jones
Wed Aug 22, 2018 3:07 am
Forum: Microscopes and optics
Topic: Magnification Changer
Replies: 7
Views: 2004

Re: Magnification Changer

In addition to apochronaut's comments, mag changers also can be quite useful to aid in framing your photographs or videos in that they will allow you to more easily fill the sensor frame so you don't lose as much as when cropping in post. Particularly handy when shooting video as cropping in post is...
by Tom Jones
Tue Aug 21, 2018 2:57 pm
Forum: Identification help
Topic: new vedios!! Yeast or Parasite in my Stool???
Replies: 9
Views: 2457

Re: new vedios!! Yeast or Parasite in my Stool???

Some yeast in stool is normal. If you're sick and think you might have parasites :o , you might want to go to your physician and ask for a stool culture and Ova and Parasite Exam. Have a real clinical lab go through the proper bacterial culturing, O&P concentration and staining procedures. You proba...
by Tom Jones
Tue Aug 14, 2018 2:39 am
Forum: Illumination Techniques
Topic: How is this not DIC? Oblique illumination? HOW?
Replies: 10
Views: 3962

Re: How is this not DIC? Oblique illumination? HOW?

He explains his technique in the comment section of another video: https://www.youtube.com/watch?v=7xvkEssi-Ss He doesn't use a patch stop, but de-centers the Abbe condenser, and sometimes uses a Raynox DCR-250 macro lens sitting on top of the illumination port. Oblique effect is obtained by condens...
by Tom Jones
Wed Jul 04, 2018 3:50 am
Forum: Beginner's corner
Topic: Replacement 100x Objective for Olympus cx31
Replies: 6
Views: 1483

Re: Replacement 100x Objective for Olympus cx31

Marty, The new price they quoted isn't that bad, and might be your best bet if you have a decent relationship with the vendor. I wouldn't go with an Amscope lens because you'll be mixing the Amscope objective optics with the Oly tube lens. Being physically interchangeable and optically interchangeab...
by Tom Jones
Tue Jul 03, 2018 1:28 am
Forum: Beginner's corner
Topic: Replacement 100x Objective for Olympus cx31
Replies: 6
Views: 1483

Re: Replacement 100x Objective for Olympus cx31

Marty,

Let me check my spares. I *think* I have one I could make you a deal on. Hopefully I can check tomorrow.

Tom
by Tom Jones
Sun Jun 17, 2018 2:43 am
Forum: Specimens, samples and slides
Topic: Formula for glycerin gelatin mounting medium
Replies: 14
Views: 7614

Re: Formula for glycerin gelatin mounting medium

JackyMac

The glycerine, gelatin and formalin add up to 67%. I presume the other 33% is water?

Tom
by Tom Jones
Wed Apr 18, 2018 2:40 pm
Forum: Beginner's corner
Topic: First time buyer - looking for benefits and pitfalls of various models
Replies: 22
Views: 3268

Re: First time buyer - looking for benefits and pitfalls of various models

Technicolor, Let the homework begin! I see you are in LA. I'm East in Redlands. Since it's really hard to figure out what you want to do without a bit (or more) of hands-on experience with some of the microscopes available, I thought you might like the opportunity to take a closeup look at some of t...
by Tom Jones
Thu Apr 05, 2018 3:44 am
Forum: Off Topic
Topic: How can eBay seller 'have it's cake, and eat it too?'
Replies: 14
Views: 2889

Re: How can eBay seller 'have it's cake, and eat it too?'

They can't. I expect an item to be as the Ebay description states. Ebay has very clear descriptions, and sellers are held to them. Used does NOT mean "as is" regardless of what the seller says. If they say "for parts or repair" then you're done. They can't sell crap as "used" and hide behind "as is"...
by Tom Jones
Tue Mar 27, 2018 1:15 am
Forum: My microscope
Topic: Reichert Zetopan?
Replies: 5
Views: 2449

Re: Reichert Zetopan?

KB1FRO,

Definitely a Zetopan. Better yet, the Ph A (yellow band) objectives are Anoptral Phase Contrast :o . They're pretty rare, and should do quite nicely if you have a Zetopan phase condenser.

Tom
by Tom Jones
Tue Mar 06, 2018 5:04 pm
Forum: Beginner's corner
Topic: I'm a Newbie - Need help cleaning the 400x objective lens.
Replies: 3
Views: 926

Re: I'm a Newbie - Need help cleaning the 400x objective lens.

Q-tip moistened with 70% - 90% isopropanol (rubbing alcohol) works fine. Gently, with a circular motion, center to outer area. Don't reuse the Q-tips. Several passes, gently, if necessary to remove the oil and other junk. It usually takes me a half-dozen or so passes, fresh Q-tip each time, to get a...
by Tom Jones
Wed Jan 17, 2018 3:49 am
Forum: Beginner's corner
Topic: Single image science lessons
Replies: 3
Views: 1109

Re: Single image science lessons

PeteM, Way cool!! :o There's not enough of this kind of outreach!! I love it! I've done a bit, but the big shows are only a couple of days, once a year. Over 41,000 kids so far though since 2010. As you can probably tell, I like teaching the kids photomicrography with their cells phones, too. 8-) ht...
by Tom Jones
Wed Nov 29, 2017 4:26 pm
Forum: Beginner's corner
Topic: Starting with identifying a decent microscope
Replies: 24
Views: 3508

Re: Starting with identifying a decent microscope

Kurt, Yes. Q-Tips and cotton balls from the grocery store or pharmacy, but gently. Pressure can drive any hardened debris into the soft optical glass of the lens and cause a scratch. I really dislike lens tissue. People tend to just stick it on their finger and rub it flat on the objective lens, usu...