Search found 160 matches

by c-krebs
Mon Jul 11, 2016 2:19 am
Forum: Pictures and Videos
Topic: Pursuit of detail - pollen revisited - stain-attack!
Replies: 21
Views: 1213

Re: Pursuit of detail - pollen revisited - stain-attack!

John,

I'm new here but it sounds like you are having way too much fun at this. (And to think I thought all this subject preparation would be burdensome ;) )
by c-krebs
Mon Jul 11, 2016 2:17 am
Forum: Pictures and Videos
Topic: Fly eye
Replies: 13
Views: 1375

Re: Fly eye

Johann,

Nice job! Looks really good.

It is a useful technique for many opaque objects as long as you have a couple objectives with sufficient working distance to let the light work for you.
by c-krebs
Mon Jul 11, 2016 2:08 am
Forum: Pictures and Videos
Topic: Larger Diatoms Just For Fun
Replies: 25
Views: 1825

Re: Larger Diatoms Just For Fun

Kurt, These are really nice. I'll reserve judgement on the 40X (always best to make a judgement of subject that "fills-the-frame" more). The 100X looks really good. On the second image... which is excellent... you have too much of it's splendor buried in the dark tones. Check out the histogram. A si...
by c-krebs
Mon Jul 11, 2016 1:42 am
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 3556

Re: America Optical infinity optics

Any reason why you don't consider the 3 technically more resolving phase systems, such as Leitz Heine/Pv, Reichert Polyphos/Anoptral and Zeiss equivalents? Those are much more rare and expensive than AOs though. Answered your own question there Fan! ;) I like phase contrast, have used it in the pas...
by c-krebs
Sun Jul 10, 2016 11:39 pm
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 3556

America Optical infinity optics

Years ago I purchased a pretty nice AO 20. I have now perused some of the threads on the forum pertaining to AO (and some specifically talking about the AO 20). It's nice to see since these are really nice scopes and certainly can be a great value. There are some folks here that are very knowledgeab...
by c-krebs
Sat Jul 09, 2016 4:32 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 20
Views: 1551

Re: Live diatoms. Some 100X darkfield.

Any more details? These are with your olympus? I take the dark field is continous light? Are the DIC ones continous light or flash? I assume its an apo dic objective? Stacks? All with the Olympus BHS. First two are 100/1.40 S Plan Apo with a Zeiss Ultra-Condenser (oil) fitted to the stand. (That ob...
by c-krebs
Sat Jul 09, 2016 4:12 am
Forum: Pictures and Videos
Topic: Slicer returns. Initial Pics of Rosemary stalk. Johansen's Quad Stain.
Replies: 22
Views: 1171

Re: Slicer returns. Initial Pics of Rosemary stalk. Johansen's Quad Stain.

b) I haven't done any post processing , yet.... I may well do some post processing etc. and probably some crossed polaroid images etc and post these as some point. Just for fun, I grabbed image #3 (my favorite of this batch), and opened it in Photoshop. A few very quick adjustments and it was reall...
by c-krebs
Sat Jul 09, 2016 3:53 am
Forum: Pictures and Videos
Topic: SRP spiecal canal report IT'S ALIVE!!!!
Replies: 20
Views: 1531

Re: SRP spiecal canal report IT'S ALIVE!!!!

Hello MicroInspector,

The last one remains a mystery to me. :?

The first few are a freshwater amphipod, Gammarus sp. I believe. (As Rodney said...freshwater shrimp)
by c-krebs
Fri Jul 08, 2016 6:36 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 20
Views: 1551

Re: Live diatoms. Some 100X darkfield.

Rodney wrote:Oh, which camera captured those?
Rodney,
I used a Canon 3Ti (just like the one you just purchased ;) )
by c-krebs
Fri Jul 08, 2016 1:02 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 20
Views: 1551

Re: Live diatoms. Some 100X darkfield.

Really... Thanks all for the warm welcome and kind comments! You have an enthusiastic group here. I've been a photographer for about forever, but I guess its been about 12 years since I bought that Meiji ML2000 (with it's basic achromats) on Ebay and jumped into microscopy with both feet. Along the ...
by c-krebs
Thu Jul 07, 2016 8:19 pm
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 3535

Re: DSLR Camera

Hi Charles, I would very much like to get your opinion on dedicated microscope cameras, like the Olympus SC50. Do they provide same/better/worse quality images as high end DSLR cameras? Johann, I can't speak directly to your question because while I have used a couple "low-end" ones years ago I hav...
by c-krebs
Thu Jul 07, 2016 7:24 pm
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 20
Views: 1551

Live diatoms. Some 100X darkfield.

Here are some live diatoms. (Well... the forth one, Triceratium reticulum, looks to be just the frustule, but it came in with a marine sample and is in a water mount like the others) The first two are darkfield with a 100X objective. With my set-up that makes the on-sensor magnification 210X. Of all...
by c-krebs
Thu Jul 07, 2016 1:19 am
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 3535

Re: DSLR Camera

Charles, What do you think about the new Panasonic GH4, which has EFSC and 4K video? Are there any other micro 4/3 that you would recommend? For afocal - camera lens - scope eyepiece coupling, which F stop number do you recommend for camera lens? I haven't really studied most of the 4/3 cameras in ...
by c-krebs
Wed Jul 06, 2016 10:00 pm
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 3535

Re: DSLR Camera

Rodney, Can I ask what microscope "coupling" you intend to use? What microscope? The reason I ask is that there are a couple very attractive MFT ("micro four thirds") available. These afe mirrorless but can have some real advantages on a scope. There are some characteristics about the microscope set...
by c-krebs
Wed Jul 06, 2016 8:19 pm
Forum: Pictures and Videos
Topic: Spring water.
Replies: 13
Views: 1189

Re: Spring water.

Nice shots! See these fairly regularly in old samples. Definitely "left-overs" from decayed plant matter. See the second image (a photomicrograph) on this page http://waynesword.palomar.edu/trjune99.htm for a pretty good look at them in situ. I'll leave it to the plant experts like John to tell us t...
by c-krebs
Wed Jul 06, 2016 7:55 pm
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 6336

Re: Exorcising a Vibration Demon

Kurt, Personally I'm leery about the T6i (and T6s) after some reports and images about a potential problem with microscopy and astro-photography. See this thread. "pwnell" is a good photomicrographer and very particular, so I thought perhaps he was looking "too hard", and maybe altering contrast way...
by c-krebs
Wed Jul 06, 2016 7:14 am
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms
Replies: 19
Views: 1879

Re: Tube-dwelling diatoms

You mention your setup is modified for electronic flash. Do you mind sharing how this is done? I can only imagine the improvement a flash would make when imaging fast moving specimens. I've used about 4 different methods over the years. The one I use now is for my Olympus BHS. This stand has a quic...
by c-krebs
Wed Jul 06, 2016 6:14 am
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 6336

Re: Exorcising a Vibration Demon

Kurt, The T3i, as with the other digital Rebels, always uses EFSC when a continuous light exposure is made from live-view. There is no setting to turn it on or off. (Different story when using a Canon electronic flash). With the 50D and other"xxD" series Canons, as well as the FF models you need to ...
by c-krebs
Tue Jul 05, 2016 8:37 pm
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 6336

Re: Exorcising a Vibration Demon

I need to replace a Canon, and am watching the new 80D's prices creep downwards. I wonder if anyone has tested the shutter Was reading through this thread since "vibration" has been a constant nemesis in microscope photography. I can offer some info to "Interference" in regards to his question abou...
by c-krebs
Tue Jul 05, 2016 3:14 am
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms
Replies: 19
Views: 1879

Re: Tube-dwelling diatoms

Fill us in on the obj's used Darkfield is 10/0.40 #2 is 20/0.70 #3 is 40/0.95 #4 is 60/0.14 #5 is 40/0.95 All Olympus S Plan Apo on an Olympus BHS stand with lamphouse modified for electronic flash. Olympus 1.67X NFK projection photo-eyepiece. Camera was either Canon 50D or T3i. - is it safe to ass...
by c-krebs
Mon Jul 04, 2016 10:34 pm
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms
Replies: 19
Views: 1879

Re: Tube-dwelling diatoms

Thanks all!

In the upper left hand corner of the last image is the marine diatom Ditylum brightwellii. (They always remind me of some sort of "communication satellite" floating in space ;) ).
by c-krebs
Mon Jul 04, 2016 8:42 pm
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms
Replies: 19
Views: 1879

Tube-dwelling diatoms

Whenever collecting marine specimens (or in rapidly moving fresh water) I always keep an eye out for what looks like a very delicate brown alga. Under the microscope this alga sometimes turns out to actually be tube dwelling diatoms. The diatoms live in tubes of mucilage. I find it fascinating how t...
by c-krebs
Mon Jul 04, 2016 8:08 pm
Forum: Pictures and Videos
Topic: Sonchus - the new batch!
Replies: 36
Views: 3025

Re: Sonchus - the new batch!

Nice work indeed!
I also appreciate the "behind the scenes" shots and the close-ups of the un-sectioned specimens. Sometimes with a high mag shot of a plant section it can be hard to grasp what one is looking at.
by c-krebs
Mon Jul 04, 2016 8:05 pm
Forum: Pictures and Videos
Topic: tissue sample
Replies: 25
Views: 1703

Re: tissue sample

MicroInspector wrote: And try to take better photos
Tell what you think?
I'm new here and not familiar with your "hardware". Are there any posts that provide some info on the equipment you are using?
by c-krebs
Mon Jul 04, 2016 7:57 pm
Forum: Pictures and Videos
Topic: Attempts at DF and Oblique DF imaging using a DF condenser
Replies: 6
Views: 616

Re: Attempts at DF and Oblique DF imaging using a DF condenser

As 75RR mentioned, it might be nice to have a brightfield "baseline" to get some bearings. But it certainly appears to to be providing better detail.
by c-krebs
Mon Jul 04, 2016 7:54 pm
Forum: Pictures and Videos
Topic: Epithemia, Frustulia, Gyrosigma
Replies: 14
Views: 1130

Re: Epithemia, Frustulia, Gyrosigma

Rod,

Darkfield at these magnification is no "piece of cake".... well done!
How do you find that AO 50X, I've heard good things about it.
by c-krebs
Mon Jul 04, 2016 7:51 pm
Forum: Pictures and Videos
Topic: Algues eau douce
Replies: 7
Views: 727

Re: Algues eau douce

These are small, nicely images! Scale bars are so valuable with subjects like this where many viewers (like me) have no familiarity with the subject.
by c-krebs
Mon Jul 04, 2016 7:47 pm
Forum: Pictures and Videos
Topic: Damaged alga or Vagincola?
Replies: 23
Views: 2789

Re: Damaged alga or Vagincola?

Looks to me like an empty peritrich ciliate lorica (could be Vaginicola) that has been "occupied" by some opportunistic squatters... what they are I don't know.

Nice clean, clear DIC image!
by c-krebs
Mon Jul 04, 2016 7:38 pm
Forum: Pictures and Videos
Topic: Iowa Diatoms In Texas
Replies: 61
Views: 4555

Re: Iowa Diatoms In Texas

Kurt, Nice variety. Some real small ones in there... the 100X DIC shows very good contrast. One thing has me curious... If the EXIF data are correct, then these were shot at ISO 1600 at about 1/30 second. I also use a T3i, and generally try to avoid boosting the ISO that high unless needed for movin...
by c-krebs
Thu Jun 30, 2016 8:18 pm
Forum: Pictures and Videos
Topic: Ant eyes
Replies: 22
Views: 2817

Re: Ant eyes

In your opinion how do these semi-apochromatic objectives compare say to a Nikon BD plan 20X or an Nikon M-plan 20X? The na does not appear to be that different although the CA would vary. In the past I believe you favored Olympus Splanapo 160 TL objectives . Although they have the same FN and bett...