Search found 2407 matches

by MicroBob
Tue Jan 26, 2021 9:01 pm
Forum: Pictures and Videos
Topic: Broom sections in different stains
Replies: 5
Views: 114

Re: Broom sections in different stains

Hi John, my biology knowledge is average at best and these differentiating stains give me at least a little guidance towards what is what. I made some test sections from ivy leaf stems now. The stems didn't cut very well, fresh a bit better than fixed. Problem with the test mixes is that the stained...
by MicroBob
Tue Jan 26, 2021 8:20 am
Forum: Collecting microscopes and slides
Topic: rathenow photo microscope
Replies: 4
Views: 91

Re: rathenow photo microscope

Hi together, ROW (Rathenower Optische Werke) was the new name of Emil Busch Optische Werke after 1945 in the GDR. Emil Busch hat a huge impact on worldwide optics industry by inventing lens grinding machines that allowed to produce precision lenses at a fraction of the cost of before. They were in t...
by MicroBob
Mon Jan 25, 2021 8:51 pm
Forum: Introduce yourself
Topic: Hello everyone
Replies: 2
Views: 57

Re: Hello everyone

Hi Howard,
welcome to the forum!
You video already looks quite good. For further improvement a video editing software would be useful to cut out video frames without much content. This can be done fairly easy with e.g. Magix.

Bob
by MicroBob
Mon Jan 25, 2021 8:42 pm
Forum: Introduce yourself
Topic: Hello
Replies: 5
Views: 73

Re: Hello

Hi,
welcome to the forum! You probably will find quite a few interesting things here.
With which name can we address you?
I have attatched an image of a section, cut with a razor blade from an Ilex leaf. It is stained with school fountain pen ink. Something like this is failrly easy to do.

Bob
by MicroBob
Mon Jan 25, 2021 8:35 pm
Forum: Specimens, samples and slides
Topic: Clear nail polish
Replies: 18
Views: 605

Re: Clear nail polish

As for the White Pine resin I collected and used. It was okay. The trees did not produce very much and what little they did produce, was just as good as Canada Balsam. The only problem was that because it was not filtered it did contain tiny pieces of organic material. If one had the proper resourc...
by MicroBob
Mon Jan 25, 2021 3:13 pm
Forum: Pictures and Videos
Topic: Broom sections in different stains
Replies: 5
Views: 114

Re: Broom sections in different stains

The sections were cut with a nice japanese table microtome, like a hand microtome in a little stand. It is a bit more practical than my hand microtome with desk holder but largely the same. for sectioning I used the 3D-printed blade holder I designed. It offers a very steady support for the blade an...
by MicroBob
Mon Jan 25, 2021 8:53 am
Forum: Specimens, samples and slides
Topic: How do you measure thickness?
Replies: 6
Views: 125

Re: How do you measure thickness?

Hi Kevin, if this were a metric hand microtome it would advance 0,5mm per turn. The screw cap at the bottom only advances the piston, it doesn't retract it. So to carry out Pete's test it would be best to start in a low position and turn the piston upwards. The hand microtomes available today often ...
by MicroBob
Sun Jan 24, 2021 8:30 pm
Forum: Pictures and Videos
Topic: Broom sections in different stains
Replies: 5
Views: 114

Broom sections in different stains

Hi toghether, here a couple of photos resulting from experiments to re-invent an old black stain for botanic sections. The original was said to specifically stain unlignified cell walls. The new stain does this but more experimentation is needed to find good combination stains. One unexpected proble...
by MicroBob
Sun Jan 24, 2021 7:30 pm
Forum: Specimens, samples and slides
Topic: How do you measure thickness?
Replies: 6
Views: 125

Re: How do you measure thickness?

Hi Kevin,
when I cut sections and advance e.g. 40µ per section, get a section with each cut and all sections look the same - they have a thickness of 40µ, precise enough.
What is the background of your question?

Bob
by MicroBob
Sun Jan 24, 2021 4:04 pm
Forum: Beginner's corner
Topic: Rule number 8 clarification
Replies: 6
Views: 143

Re: Rule number 8 clarification

These offered services can lead to a lot of distress as desparate people with illnesses like cancer have themselves treated by someone who is probably not up to the job. Our user apochonaut once wrote a very interesting answer according to this topic: http://www.microbehunter.com/microscopy-forum/vi...
by MicroBob
Sun Jan 24, 2021 12:25 pm
Forum: My microscope
Topic: 3 old microscopes
Replies: 14
Views: 933

Re: 3 old microscopes

Hi Jim,
it is very valuable to be able to make repairs and additions for the microscope and surrounding equipment. Cheap new and professional used equipment is often 90% good but needs the last 10% rectified.

Bob
by MicroBob
Sun Jan 24, 2021 6:43 am
Forum: Microscopes and optics
Topic: Scratches or sticky residue and should I worry
Replies: 2
Views: 88

Re: Scratches or sticky residue and should I worry

Hi, I would interpret this as dried immersion oil and a little dust that is stuck in it. The 40:1 has little free working distance and often gets dunked in immersion oil. Many plan objectives have a concave front lens that is a bit more difficult to clean in the middle. So I would try to check whete...
by MicroBob
Sun Jan 24, 2021 6:39 am
Forum: Specimens, samples and slides
Topic: Clear nail polish
Replies: 18
Views: 605

Re: Clear nail polish

Hi Red_Green, do you have access to xylene, the common intermedium for canadabalsam? Half a year ago you wrote about using the sap of your white pine tree as a montant - how well have these slides lasted so far? Price wise the canadabalsam you linked is quite ok as it is a very big bottle. If one co...
by MicroBob
Sat Jan 23, 2021 6:41 pm
Forum: My microscope
Topic: 3 old microscopes
Replies: 14
Views: 933

Re: 3 old microscopes

Hi Jim,
I can see the uploaded image. Nice mofification! Did you make the metal parts yourself?

Bob
by MicroBob
Sat Jan 23, 2021 1:31 pm
Forum: Specimens, samples and slides
Topic: Collection management and study of microscope slides
Replies: 6
Views: 112

Re: Collection management and study of microscope slides

Hi Tom,
sorry, my fault, the link works fine!

Bob
by MicroBob
Sat Jan 23, 2021 8:53 am
Forum: Camera systems and imaging
Topic: Chinese Unbranded 48mp C-mount Industrial HDMI Camera
Replies: 26
Views: 359

Re: Chinese Unbranded 48mp C-mount Industrial HDMI Camera

Hi John, I was able to watch the video inside google drive with up to 1080 resolution and was offered the download. I didn't download it as I didn't want to have that 2,2 GB file on my already full computer. In my view 1080p should do for all purposes, it offers what the eye can resolve, and on bigg...
by MicroBob
Sat Jan 23, 2021 8:23 am
Forum: Microscopes and optics
Topic: More Photomic iii questions- power supply/light
Replies: 6
Views: 138

Re: More Photomic iii questions- power supply/light

Hi, I have a Phomi 1, second generation fom the 60s and don't know the differences to the Phomi 3 in every detail. For my microscope the "ABTO" box supplies a little power for the lamp and has certain controls for the camera. While the analog camera image quality of the Phomi 3 is said to be extreme...
by MicroBob
Fri Jan 22, 2021 9:02 pm
Forum: Specimens, samples and slides
Topic: Collection management and study of microscope slides
Replies: 6
Views: 112

Re: Collection management and study of microscope slides

Hi Tom,
your link didn't work for me.
I was able to access it here: https://opus4.kobv.de/opus4-bam/files/42332/Zootaxa.pdf

Corrected - Link works as it should!
The article includes lots of information on the long term stability of almost all known mountants - very interesting.

Bob
by MicroBob
Fri Jan 22, 2021 6:59 am
Forum: Miscellaneous
Topic: Vibration dampening
Replies: 10
Views: 217

Re: Vibration dampening

After a few calm years he will become very strong! When I look into my office/lab the biggest problem would be chemicals, hot plates, special electric stuff and microtome and other very sharp knives. From 3 on it wouldn't take long any more to make a child understand that the room is off limits, but...
by MicroBob
Fri Jan 22, 2021 6:28 am
Forum: Microscopes and optics
Topic: objective comparison
Replies: 3
Views: 112

Re: objective comparison

Hi together, I think the Leitz standards of "planar" have changed over the years so the genereation of objective has to be taken into account. The first planar objective series from about 1960 consisted of fairly plain looking objectives with highly reflective chrome. This was pre-Orthoplan, so no n...
by MicroBob
Thu Jan 21, 2021 9:01 pm
Forum: Specimens, samples and slides
Topic: Staining lichen
Replies: 3
Views: 70

Re: Staining lichen

Hi Mike, on our website you can find Schömmers "Kryptogamenpraktikum": http://www.mikrohamburg.de/Schoemmer/Schoemmer.pdf The name of the book is difficult to decipher even for me as a german, but the book itself is an insider tip. When you look for "Flechten" = lichen, you will find the right chapt...
by MicroBob
Thu Jan 21, 2021 4:31 pm
Forum: Microscopes and optics
Topic: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long
Replies: 12
Views: 262

Re: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long

Hi Doron, I thought of a thermocouple as they are small. The more common PT or NTC sensors would be too big to fit next to the front lens. I don't know the electrical resistance of thermocouples, but a resistance measurement might help to narrow down the selection of opportunities. These could also ...
by MicroBob
Thu Jan 21, 2021 4:27 pm
Forum: Pictures and Videos
Topic: Desmid: Closterium littorale
Replies: 5
Views: 172

Re: Desmid: Closterium littorale

Hi Carlos,
nice video!
What I find fascinating are these revolving gypsum crystals in the tips.

Bob
by MicroBob
Thu Jan 21, 2021 4:16 pm
Forum: Illumination Techniques
Topic: scan lines in my camera image
Replies: 3
Views: 127

Re: scan lines in my camera image

Hi Don, your incandescent lamp should be powered with current that is rectified and smoothed by a condensator - with proper DC power it doesn't flicker. LEDs can go on and off in an instant an can flicker severely if not powered by smoothed DC. Sometimes LED lamps are powered just with rectified AC ...
by MicroBob
Thu Jan 21, 2021 4:04 pm
Forum: Miscellaneous
Topic: Vibration dampening
Replies: 10
Views: 217

Re: Vibration dampening

Hi, I would suggest a three step solution: - fat steel plate under the microscope (4x the denstity of concrete) e.g. 400x400x40mm = 50kg - foam rubber under the steel plate, amount chosen so the rubber already starts to be compressed, perhaps 50mm thick uncompressed - velcro loop carpet and a velcro...
by MicroBob
Thu Jan 21, 2021 7:26 am
Forum: Microscopes and optics
Topic: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long
Replies: 12
Views: 262

Re: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long

Interesting catch!
With all the heating it would make sense to measure the temperature. Is it possible that the tiny elements next to the front lens are a thermocouple? They put out a tiny voltage, perhaps you can measure it.

Bob
by MicroBob
Wed Jan 20, 2021 9:53 pm
Forum: Camera systems and imaging
Topic: Chinese Unbranded 48mp C-mount Industrial HDMI Camera
Replies: 26
Views: 359

Re: Chineses Unbranded 48mp C-mount Industrial HDMI Camera

Hi John, apparently the chinese printed all necessary information on the outside of the housing, perhaps there is a side with closer instructions? What I can see from the print on the housing is that it is powered by a V8 motor and at 4800W it consumes quite some energy, not for a V8 but for a camer...
by MicroBob
Tue Jan 19, 2021 3:23 pm
Forum: Beginner's corner
Topic: Inverted inquiry
Replies: 7
Views: 261

Re: Inverted inquiry

I just have a basic Olympus Ck with max 20:1 objective and only coarse focus and bicycle lamp. It can be used through the bottom of a slide or petri dish. There are higher power objectives that work through the bottom of petri dishes, like the 32:1 Leitz, but the higher the power the rarer these obj...
by MicroBob
Mon Jan 18, 2021 9:19 pm
Forum: Specimens, samples and slides
Topic: Desmids after 7 months in an Eppendorf tube
Replies: 2
Views: 217

Re: Desmids after 7 months in an Eppendorf tube

Hi Glen, yes, like the right one, 2ml. The lid closes water proof and clicks in. I got the tube by post in an ordinary paper envelope. Apparently there is a recycling process running with very little waste. Some desmids reproduce very slowly so some of these might actually be from the first generati...
by MicroBob
Mon Jan 18, 2021 7:03 am
Forum: Beginner's corner
Topic: Need help figuring out which compound microscope I should buy.
Replies: 5
Views: 217

Re: Need help figuring out which compound microscope I should buy.

Hi Mondo, microscope choice is discussed in this forum frequently and many people look for the same thing as you, a usable microscope to start with, not too limiting, not too expensive. So you might have a look what has been recommdended in the past. Camera choice is very much a question of expected...