Search found 2401 matches

by MicroBob
Tue Dec 08, 2020 9:11 pm
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 754

Re: Question about setting up Zeiss DIC

Hi, Zeiss said to use oil immersion on the condenser front lens at all magnifications for your DIC system. Water immersion comes close to oil immersion and it works dry too, but of cause with drawbacks. When using immersion make sure that no fluid enters the fine thread that attatches the top lens. ...
by MicroBob
Tue Dec 08, 2020 8:33 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 827

Re: Experiments with botanic stains

In the german forum the Wacker W3A variations are very popular, actually have been varied, developed and improved by a couple of members. These are very very good stains but as well as Etzold FCA they are quite colourful, over time may be a bit overly colourful. When Rolf-Dieter-Müller, one of the s...
by MicroBob
Tue Dec 08, 2020 5:18 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 827

Re: Experiments with botanic stains

I have showed my hand /cylinder microtome and the blade holder I have developed here. I actually have a hand microtome for sale.

viewtopic.php?f=16&t=7065&p=62601&hilit ... der#p62601
by MicroBob
Tue Dec 08, 2020 4:29 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 827

Re: Experiments with botanic stains

Hi Wes,
this was cut embedded in a slit carrot with a cylinder microtome with my 3D-printed blade holder. The leaf sectioned very well.
The SFC-mix geves the yellow, the ink the black and blue tones.

Bob
by MicroBob
Tue Dec 08, 2020 3:59 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 827

Experiments with botanic stains

Hi together, in former times there was a stain called "Kernschwarz" of unknown formulation. It stained unlignified cell walls very specifically. Today it is not available any more but it is thought to be based on gallic acid and an iron salt, like iron gall ink. This made me think how iron gall ink ...
by MicroBob
Sun Dec 06, 2020 7:11 pm
Forum: Illumination Techniques
Topic: Oblique filter suggestions
Replies: 12
Views: 1245

Re: Oblique filter suggestions

Yes, I placed it in the filter holder unter the condenser. When I place one of the aluminium discs on the acrylic glass disc I get a hollow cone of light from the gap between outside aluminium disc and inside filter holder. These filter holders are placed not alike. The position doesn't matter much ...
by MicroBob
Sun Dec 06, 2020 11:32 am
Forum: Illumination Techniques
Topic: Oblique filter suggestions
Replies: 12
Views: 1245

Re: Oblique filter suggestions

Hi Glen, I dug this out for you. The aluminium discs are from 0,4mm foil, punched out with an expensive french double punch set on a piece of wood end grain. Oblique and COL are probably let down by their improvised use. If one would dedicate a turret condenser with stops for specific objectives jus...
by MicroBob
Sun Dec 06, 2020 11:15 am
Forum: Pictures and Videos
Topic: Ilex leaf stem hand sections stained with Etzold FCA and Wacker W3ASim
Replies: 4
Views: 346

Re: Ilex leaf stem hand sections stained with Etzold FCA and Wacker W3ASim

The video needs some additions and then I will think about making it public. I think about adding good photos mit inscriptions for the functional tissues. But my slides are still drying and I want them better cured before I take good photos. When I make the video public I will post a link of cause. ...
by MicroBob
Sat Dec 05, 2020 9:05 pm
Forum: Pictures and Videos
Topic: Ilex leaf stem hand sections stained with Etzold FCA and Wacker W3ASim
Replies: 4
Views: 346

Ilex leaf stem hand sections stained with Etzold FCA and Wacker W3ASim

Hi together, in preparation of next weeks virtual group meeting I prepared a video on making free hand sections and staining and mounting them. Ilex leaves are very hard and tough on the outside but a bit foam like on the inside, at least when harvested at this time of year. These are stained with E...
by MicroBob
Sat Dec 05, 2020 8:55 pm
Forum: Illumination Techniques
Topic: Oblique filter suggestions
Replies: 12
Views: 1245

Re: Oblique filter suggestions

Hi, oblique lighting is a bit fiddly to set up and difficult to repeat. This and the fact that it tends to introduce arifacts probably lead to the low use in professional science work today. I use what is at hand, often the filter holder or one of my fingers. I heard that decentering an auxillary le...
by MicroBob
Fri Dec 04, 2020 1:24 pm
Forum: Pictures and Videos
Topic: Micro hedgehog
Replies: 15
Views: 3588

Re: Micro hedgehog

Hi together, I have just found a video I took when Fritzi the little Hedgehog was about 6 weeks old: https://vimeo.com/487214021 This was about his daily food, about 40% of his body weight! They are supposed to gain 10g each day until they are big and fat enough to be put into hibernation. We woke F...
by MicroBob
Thu Dec 03, 2020 7:18 am
Forum: Introduce yourself
Topic: Use of Potassium Hydroxide
Replies: 2
Views: 564

Re: Use of Potassium Hydroxide

Hi Antoni,
no practical experience here but you might use Hoyers mix or clove oil to mount your insects.

Bob
by MicroBob
Thu Dec 03, 2020 7:16 am
Forum: Microscopes and optics
Topic: Unknown terms related to DIC
Replies: 11
Views: 825

Re: Unknown terms related to DIC

Perhaps "warrior" is the result of an automatic translation of "wollaston".
Epi DIC might speed up the evaluation of indentations in good/bad quality assurance.
by MicroBob
Wed Dec 02, 2020 3:36 pm
Forum: Microscopes and optics
Topic: Unknown terms related to DIC
Replies: 11
Views: 825

Re: Unknown terms related to DIC

Hi Phil,
I have never heard these terms so can't help. I would say it is unlikely that they have to do with the finity Zeiss West systems.

Bob
by MicroBob
Tue Dec 01, 2020 4:45 pm
Forum: Pictures and Videos
Topic: Leaf casts (aka Leaf impressions)
Replies: 12
Views: 781

Re: Leaf casts (aka Leaf impressions)

Good idea and nice result! The staining probably reduces the contrast to a more managable level for the sensor. I don't know much about this but stains have different mechanisms to connect to certain materials and structures. To some degree this can be explained by the chemical properties of the sta...
by MicroBob
Tue Dec 01, 2020 4:27 pm
Forum: Microscopes and optics
Topic: Zeiss Achromat-Aplanat 465277 turret condenser - help needed
Replies: 14
Views: 794

Re: Zeiss Achromat-Aplanat 465277 turret condenser - help needed

Hi Doron, nice to see that you got your condenser to work again! These are really impressively made condensers and for the age and no proper maintenance for a long time the condition is actuall quite good. The phase annulli seem do be damaged at the outer surface only, rigth? Then the repair is fair...
by MicroBob
Tue Dec 01, 2020 6:55 am
Forum: Illumination Techniques
Topic: Oblique lighting and florescence lamp recommendation.
Replies: 3
Views: 417

Re: Oblique lighting and florescence lamp recommendation.

The light amount of fluorescence is usually small. Under a stereo microscope you already need a lot of illumination for the higher powers. So probably a fluorescence illumination with sideways light will only give a dim image with weak objectives. Probably you better look for an epi fluorescence mic...
by MicroBob
Tue Dec 01, 2020 6:52 am
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

Kionka wrote:
Tue Dec 01, 2020 6:24 am
The black borders are because of cross polarization Bob.
Okay, I didn'T have this in mind. To check image quality I think plain bright field is better.
by MicroBob
Mon Nov 30, 2020 6:50 pm
Forum: Beginner's corner
Topic: Cleaning slides lifehack
Replies: 7
Views: 575

Re: Cleaning slides lifehack

Hi, a 100x slide box without lid and with added hole sawed holes in the bottom can be used in the dish washer. Best results with fairly clean and freshly used slides and an empty dishwasher without any greasy stuff inside. Check first whether the slide boxes survive the temperature in the dish washe...
by MicroBob
Mon Nov 30, 2020 3:05 pm
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

What puzzles me: Why do the images have a black border? To me this looks as if the section is nat fully illuminated.

Bob
by MicroBob
Sun Nov 29, 2020 8:03 pm
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

The Plan 2,5 is quite a nice objective, see here: https://www.mikroskopie-forum.de/index.php?topic=39220.0 The image in the link is better than I would have thought possible with a single shot and this objective. But this doesn't happen by itself. The usual condensers are not really optimized for th...
by MicroBob
Sun Nov 29, 2020 7:27 pm
Forum: Pictures and Videos
Topic: Help with improving image
Replies: 17
Views: 1001

Re: Help with improving image

Camera used is a Nikon d810 using mirror up and front curtain shutter. Hi Ian, do you mean you used first elecronic shutter curtain function? Shutter shock can do bad things to image sharpness. My experience with stacking: Contrary to my expectations stacking doesn't always increase image content. ...
by MicroBob
Sun Nov 29, 2020 5:50 pm
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

Is that made with a 2,5:1 objective? And is it a plan objective?
by MicroBob
Sat Nov 28, 2020 6:10 pm
Forum: Beginner's corner
Topic: STAINS.. Sooo many these days
Replies: 7
Views: 689

Re: STAINS.. Sooo many these days

Charles wrote:
Sat Nov 28, 2020 3:06 pm
We always used staining racks and forceps to keep from getting stained fingers. You can also use gloves.
Hi Charles, this is part of the lab method know how that is useful to use critical stuff in a safe way. So of cause it can be handled safely but one has to be set up in a sensible way.

Bob
by MicroBob
Sat Nov 28, 2020 6:03 pm
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

If the eyepice is parfocal when sitting in the photo port it won't project an image on you sensor up there. When you lift it up ca. 10mm you might get parfocality.

Bob
by MicroBob
Sat Nov 28, 2020 11:14 am
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

Hi, in theory the 0,17mm instead of 1,5mm glass layer will introduce abberations to some extent. But as you already have it I would suggest to just compare it with the weaker and stronger objectives you have an see how well it performs. I would also look for parfocality and especially check for imag...
by MicroBob
Sat Nov 28, 2020 8:17 am
Forum: Pictures and Videos
Topic: Leaf casts (aka Leaf impressions)
Replies: 12
Views: 781

Re: Leaf casts (aka Leaf impressions)

It probably is worth an experiment to slightly stain the glue. This way you might have it easier to get a good image.

Bob
by MicroBob
Sat Nov 28, 2020 8:14 am
Forum: Specimens, samples and slides
Topic: Slide Prep Techniques for Nematodes
Replies: 4
Views: 419

Re: Slide Prep Techniques for Nematodes

Hi Bruce, you might have a look whether Hoyers mix would be applicable here. Otherwise you probably have to bring your neamtodes through an alcohol row into xylene and from there into canadabalsam or Malinol. Classic nail polish contains a lot of solvent and shinks too much for thicker objects. Stai...
by MicroBob
Fri Nov 27, 2020 3:52 pm
Forum: Microscopes and optics
Topic: Do I need to change the rack of my Leica microscope ?
Replies: 5
Views: 457

Re: Do I need to change the rack of my Leica microscope ?

I would suggest to also clean the bottom of the cog teeth with a tooth pick. There might be bits of cover slip embedded in the plasic. When mounting the rack again just fasten the screws loosely, then move to one end, apply light pressure towards the cog, tighten a bit more, move to the other end, a...
by MicroBob
Fri Nov 27, 2020 12:40 pm
Forum: Pictures and Videos
Topic: Leaf casts (aka Leaf impressions)
Replies: 12
Views: 781

Re: Leaf casts (aka Leaf impressions)

Hi Michael,
this might be similar to "UHU Hart": https://www.uhu-profishop.de/sicherheit ... rkblaetter
Which is available in DIY and model making shops here in Germany.
Bob