MicrobeHunter.com Microscopy Forum

And the filaments look really interesting when used for a while....

Hi Kurt,
beautiful photos again! In a chemically cleaned sample some of these diatoms would be difficult to find.

Bob

Hi Steve, a dichroic mirror is used for fluorescence work, a half silvered mirror for normal epi immumination. A cover slip or foil works as a half silvered mirror, but only a little part is reflected down, the rest ends possibly as stray light. So a real half silvered mirror improves image quality ...

How about this half silvered mirror: https://de.aliexpress.com/item/40011567 ... 4c4dQBVd0T

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Hi Timur,
very impressive photo! I actually printed the middle section on my postcard printer. A second print after "dehaze" with Rawtherapee was even better.
Who would think of so much beauty in a light bulb?

Bob

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Which colour does the stop have? PLA or PETG in black might become too warm just from the absorbed visible light. In this case a layer of aluminium foil would solve the problem.

Then you could think about a Lytkarino MBS10, often misnamed LOMO. It is (as much russian optics) based on a Zeiss Jena design, similar to the black Zeiss you showed. They are nice to use with the lower and medium magnifications, the highest magnification is not that sharp any more, but no big limit...

In my experience the condition of the stereo microscopes varies more than their capabilities in good shape. Many have been used in metal or dental workshops, production environments or for soldering and are foggy enough to need a bottle of rum when using them. The Zeiss West/Oberkochen model is a ne...

Hi Michael, the front of the base is 96mm long. You should be able to find the other dimensions as close as necessary from there. The dovetail ways are ground, it would be even better to scrape them. When building them it would be heltpul to choose good bearing partners, e.g. all cast iron. These sl...

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Hi together, here some more photos. The next cut is set when the block holder moves upwards, short before the end pf the upwards stroke. The long element in front is part of a section transport mechanism. The microtome used to have a housing, I'm not going to build one for it as the mechanism is eas...

I have two prices for it. In 1913 it was about 2/3 of a microscope with two objectives and mono tube. In 1978 it was said to be priced like an SLR camera with 2 objectives. It kept it's value in a similar way as these references.

I can take a few more photos of cause.

These microtomes have been made for decades but rarely surface on ebay. It may have been in the catalog to impress customers without actually making money with it. There are stamped numbers "10" and "248", so probably very small series. For general home use I would suggest a bigger model from somewh...

I will try to find a matching stain for you, Michael!

Hi together, I recently acquired a tiny KOSMOS rotary microtome made by E. Zimmermann. KOSMOS is a german company that traded scientific stuff for decades. These little rotary microtomes were made in several different versions for at leas 65 years. Weight is about 5kg and it lives in a nicely made w...

Hi Michael, welcome to the forum! Your Zeiss know how will for sure be valued here and a lot of interesting topics are discussed here too. The rounded nose piece of the 1938 Photomicroscope look similar to the later Zeiss Jena NU nose piece. BTW: Do you happen to have information on the buyer of the...

The critical temperature is inside the LED, not eays to measure. The LED is usually already bonded to a star board with good conductivity. The max. heat sink temperature then depends on the thermal coupling of star board to heat sink. If you attatach it with 2 screws and heat compound the heat trans...

Hi Agostino,
the question about what microscope to buy is posted in this forum in short intervals. I would suggest that you take the time to read what has been posted on this topic. Otherwise you might miss some important hints because people don't repeat them on and on.

Bob

The NPL Fluotar will do nicely, I really like these objectives on my Zeiss microscopes. The colour correction is close to that of Zeiss, you will get a good image with Zeiss eyepieces and Zeiss compatible phto adaptation. Only very critical testing can reveal the small difference. Compared to a Zeis...

It sometimes helps to fix the cover slip along one edge with tape. Most inverted microscopes have low power condensers only so the acheivable quality may not satisfy you anyway.

Have a look at Rolf's hybrid eyepiece method, in my eyes one of the best. For your Zeiss Jena microscope you can probably use a Zeiss West/Oberkochen adaptation, they were close in their colour correction approach.
https://microscopyofnature.com/micropho ... -eyepieces

Congratulations to this nice and unusual instrument. The production numers must have been small and the french government will probably have been the only customer. There is a french forum: www.lenaturaliste.fr , there will be someone who can help you with information and you may find wanted parts t...

Hi, first I would make sure where the the dust sits. When they move when you move a component, this is the dirty component. These spos show least in normal photography when you take photos with a fairly open aperture. As soon as you close the aperture down to f/22 or even smaller they will become ev...

Plancton concentration in free water is very low, so you will only get a rich sample with a net of filter. You can make a plancton net or filter yourself, I could point you in the right direction. A lot of life is usually found atattched to plants and wooden surfaces. I'm member of an old microscopy...

There are a few criteria for a good microscope camera: - Electronic first shutter cutain (NEX 3 an 5 didn't have one and the shutter blurred the images a lot, NEX 5N on had EFSC) - Tiltable screen - Tethering by PC or mobile phone if needed - Clean HDMI out if needed -.... If I would look for a came...

In disassembling multiple examples of identical objectives in order to attempt to rebuild one good one out of 2 or 3 duds, the offending cement joint is usually the same one in each objective. Bausch & Lomb 20X .50 flat field, Spencer 60X .85 dry and Zeiss 60X 1.0 oil with iris. Always the same ele...

Hi Phil, I would say it is a combination of handling, stresses and brittle glue layers that leads to delamination. So it should be possible to find delamination in unused objectives, but maybe in undetectable numbers. Another way to look at the question may be this: It probably is reasonable to expe...

Do you know how the old 170mm orthoplans are today from a usability standpoint? The impulsive half of my brain wants to buy one because they're so cool. The rational half tells me I own enough microscopes and that old German tech might be a little impractical. I don't own one but what I read from c...