Search found 2401 matches

by MicroBob
Sat Jan 16, 2021 8:16 pm
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 537

Re: Silicone immersion objectives - any experience?

Hi Viktor,
please report how you get on with this very special objective. A 40:1 1.0 will be useful for many purposes. Do you know the free working distance to the cover slip?
Now it would be nice if you can find a 100:1 silicone immersion to accompany it! :lol:

Bob
by MicroBob
Sat Jan 16, 2021 11:20 am
Forum: Beginner's corner
Topic: PH alignment without a CT
Replies: 21
Views: 448

Re: PH alignment without a CT

They are also very useful to check: - whether the back lens of the objective is clean - whether it is fully illuminated by the condenser aperture - whether there are delaminations or other defects in the lens stack of the objective Some microscopes have a Bertrand lens built in, sometimes it can be ...
by MicroBob
Sat Jan 16, 2021 9:40 am
Forum: Beginner's corner
Topic: PH alignment without a CT
Replies: 21
Views: 448

Re: PH alignment without a CT

What is a CT?
by MicroBob
Sat Jan 16, 2021 7:51 am
Forum: Microscopes and optics
Topic: Need help deciding between Motic BA310E & Panthera CC
Replies: 9
Views: 260

Re: Need help deciding between Motic BA310E & Panthera CC

Here is the contrast technique described that he uses - obviously worth a try! His images have more a 3-dimensional look than the optical sectioning effect of DIC. For plancton life his setup may very well be better auited than DIC, why not? Here the LabCam adapter types can be seen, move mouse on l...
by MicroBob
Sat Jan 16, 2021 7:32 am
Forum: Microscopes and optics
Topic: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?
Replies: 36
Views: 641

Re: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?

Hi Hans, when you put a piece of paper onto the top lens and point the bottom of the condenser towards a light source the paper should be illuminated. I think it is a good sign that no light passes through in your test as the condenser has to block it. Typical damages are paint flaking of and scratc...
by MicroBob
Fri Jan 15, 2021 4:04 pm
Forum: Microscopes and optics
Topic: Need help deciding between Motic BA310E & Panthera CC
Replies: 9
Views: 260

Re: Need help deciding between Motic BA310E & Panthera CC

The difference between the trino heads might actually be important. When you chase plancton life it is important to have an image splitter, which both have. For photography it is good to use a lot of light for low ISO settings and short exposures and the light in the eyepieces should not blind you. ...
by MicroBob
Fri Jan 15, 2021 12:41 pm
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 537

Re: Silicone immersion objectives - any experience?

Oil can be removed fairly well by wiping with lighter fluid, so objective and slides are really clean. Silicone is quite inert and doesn't dissolve in usual cleaning fluids, so you push it from left to right and back to the left. A fine silicone layer or tiny drops of silicone oil repell water and o...
by MicroBob
Fri Jan 15, 2021 11:20 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 537

Re: Silicone immersion objectives - any experience?

Hi Vikor, from the r.i. that sounds quite interesting for work on preparations with a thicker water layer. But silicone really is messy. It invisibly spreads over surrounding surfaces and interferes with wettability and adhesion. Car painters do hate it! For workshop use thin silicone oil in spray c...
by MicroBob
Thu Jan 14, 2021 8:21 pm
Forum: Home-made microscope adaptations
Topic: Slit Interference Contrast
Replies: 4
Views: 132

Re: Slit Interference Contrast

Hi,
that looks very promising!
Is your setup similar to the PZO DIC with slit condenser? PZO uses a stit aperture in the condenser and an analyser in an intermediate tube between microscope head and observation tube.
Did you see a dependency on the orientation of the diatoms?

Bob
by MicroBob
Thu Jan 14, 2021 6:05 pm
Forum: Beginner's corner
Topic: Reichert received and a question
Replies: 31
Views: 555

Re: Reichert received and a question

I just bought a pack of 10 sealed 693ZZ bearings from China for 3.17 + free shipping. Ridiculous, really. Hi Phil, I would suggest that you check the size and put a file to it to check whether they are actually hardened. I had two occasions where dimensions of chinese ball bearings were completely ...
by MicroBob
Thu Jan 14, 2021 5:34 pm
Forum: Beginner's corner
Topic: Oculars or auxiliary lens
Replies: 7
Views: 215

Re: Oculars or auxiliary lens

Hi Scarodactyl,
you must be right. I googled and Leica says their M205 has the highest n.a. of all at 0,35:

https://www.leica-microsystems.com/de/p ... -m205-fca/

What n.a. had the most powerful stereo microscope you used and how useful did you find this?

Bob
by MicroBob
Thu Jan 14, 2021 7:33 am
Forum: Beginner's corner
Topic: Oculars or auxiliary lens
Replies: 7
Views: 215

Re: Oculars or auxiliary lens

Hi together, for my 1970s Leitz Großfeld Stereocmicroscope TS I have these numeric apertures: 1,0x 0,05 1,6x 0,62 2,5x 0,8 4,0x 0,8 10x 1,0 The 1x gives an exceptionally crisp image, the 4x quite a good one, the 10x perfectly usable but not overwhelming. I don't own the others. Since the camera can ...
by MicroBob
Wed Jan 13, 2021 11:31 am
Forum: My microscope
Topic: 3 old microscopes
Replies: 14
Views: 887

Re: 3 old microscopes

I am still working out how to get jpeg pics into a format to upload Hi Jim, they have have a max. size of 1024 pixels on the long side and no more than 500kB. I use Irfan View to cut and downsize the images, just a few clicks. Then you click on "Attatchments" below and then "Add files". One problem...
by MicroBob
Wed Jan 13, 2021 11:16 am
Forum: Pictures and Videos
Topic: A marine diatom with oblique lighting
Replies: 15
Views: 412

Re: A marine diatom with oblique lighting

One problem with external image hosts is that they give up one after the other and the images are lost. Remeber Geocities or the Photobucket photo hijack? This lets the forum posts without images. In many cases these posts will be interesting in the future as others look for the same information. So...
by MicroBob
Wed Jan 13, 2021 8:57 am
Forum: Pictures and Videos
Topic: A marine diatom with oblique lighting
Replies: 15
Views: 412

Re: A marine diatom with oblique lighting

Hi Louise, the NPL Fluotars are near planar to Leitz extremely high standards. So they were planar over a 24mm! field, not 28 (?) like the Leitz "Plan" of the time. These are very good objectives, not far away from planapochromats. While 100:1 objectives on amateur microscopes are often more or less...
by MicroBob
Tue Jan 12, 2021 8:51 pm
Forum: Pictures and Videos
Topic: A marine diatom with oblique lighting
Replies: 15
Views: 412

Re: A marine diatom with oblique lighting

Leitz Wetzlar Apo 63x Your 100:1 is not that far behind, it just offers a smaller field of view. When using monochromatic light or selecting colour channels you can get rid of the disadvantages of a simple achromat. This here would be a nice task: http://www.microbehunter.com/microscopy-forum/viewt...
by MicroBob
Tue Jan 12, 2021 4:44 pm
Forum: Camera systems and imaging
Topic: Simple dslr adapter hack for budget scopes.
Replies: 9
Views: 570

Re: Simple dslr adapter hack for budget scopes.

That looks quite good to me. Just a bit of colour fringes in the corners, nothing really serious. And diatoms are a fairly critical test.
For wide flat objects you could use photo stacking to bring everything in focus without having plan objectives.

Bob
by MicroBob
Tue Jan 12, 2021 4:39 pm
Forum: Pictures and Videos
Topic: A marine diatom with oblique lighting
Replies: 15
Views: 412

Re: A marine diatom with oblique lighting

Hi Louise, that looks very good! I would guess that this was circular oblique lighting - right? Forum images can have max. 1024 pixels on the long side and 500kB file size. I haven't recognized that they are further compressed on upload. You have been very quick in getting good micro images. Some pe...
by MicroBob
Tue Jan 12, 2021 2:04 pm
Forum: Camera systems and imaging
Topic: Simple dslr adapter hack for budget scopes.
Replies: 9
Views: 570

Re: Simple dslr adapter hack for budget scopes.

Hi Louise, thank you for posting your camera adaptation! There are quite a few people who buy similar microscopes and already have a Canon DSLR so this will be interesting for them. There are also many beginning microscopists who have a background in astronomy and might even have such an astro eyepi...
by MicroBob
Mon Jan 11, 2021 3:36 pm
Forum: Pictures and Videos
Topic: Naphrax vs Pleurax vs Cameras vs Me!
Replies: 13
Views: 687

Re: Naphrax vs Pleurax vs Cameras vs Me!

Hi Louise, using the 100:1 has brought things forward a lot! I think the setup is fine like this and there is only a narrow margin for further improvements in the raw image. I have taken the blue channel, worked a bit on the histogram and did a bit of unsharp masking and the striae are visible very ...
by MicroBob
Mon Jan 11, 2021 9:27 am
Forum: Specimens, samples and slides
Topic: Amoebas dangerous ?
Replies: 7
Views: 828

Re: Amoebas dangerous ?

Hi together, I would suggest to treat this in perspective to what you and others do in everyday life. I go swimming in ponds and rivers and dig around in the garden if my wife gets hold of me. I dive in this water but avoid to swallow it. I don't wear a full body condom when I enter the water. This ...
by MicroBob
Sun Jan 10, 2021 7:37 pm
Forum: Pictures and Videos
Topic: Naphrax vs Pleurax vs Cameras vs Me!
Replies: 13
Views: 687

Re: Naphrax vs Pleurax vs Cameras vs Me!

Hi Louise, nice to see that you have started to try cleaning and mountig diatoms yourself! The results are actually not bad and there are still some points to check for possible improvement left. Small diatoms are at the border of what a light microscope can resolve in detail, so it is common to end...
by MicroBob
Sat Jan 09, 2021 8:36 pm
Forum: Beginner's corner
Topic: Wondering if this microscope kit is a good deal
Replies: 9
Views: 471

Re: Wondering if this microscope kit is a good deal

This kit is what kits are often: A collection of a lot of parts which mostly won't be used a single time. A good starting kit depends on what you will actually try to observe, but some are used all the time: - a pack of slides - cover slips, ideally in different sizes, and round and rectangular - a ...
by MicroBob
Sat Jan 09, 2021 5:37 pm
Forum: Beginner's corner
Topic: are these eyepiece in good condition ?
Replies: 3
Views: 210

Re: are these eyepiece in good condition ?

The coating might be worn or just dirty. Difficult to tell from just this photo. If the left one looks in reality like this it might clean up nicely with something like lighter fluid.

Bob
by MicroBob
Sat Jan 09, 2021 7:18 am
Forum: Beginner's corner
Topic: My first microscope
Replies: 10
Views: 626

Re: My first microscope

The fungus guys always like 100:1 oil immersion objectives. So this should be included and is with many used microscopes. For plant observations you might like to have a cylinder/hand microtome and blade holder to make sections from fresh or fixed material and stain them ( I attatch a photo of a cro...
by MicroBob
Fri Jan 08, 2021 10:23 pm
Forum: Specimens, samples and slides
Topic: Radiolaria material in block form - how to break it up!
Replies: 3
Views: 173

Re: Radiolaria material in block form - how to break it up!

Hi Doron, the high r.i. of Pleurax fits fine diatoms fine, but heavily built diatoms already appear with a bit too much contrast. So the even stronger radiolaria don't benefit from an especially high r.i.. A classic mountant would be Caedax, perhaps at maximum Caedax with a bit of Aroclor :roll: The...
by MicroBob
Fri Jan 08, 2021 10:09 pm
Forum: Microscopes and optics
Topic: Double images
Replies: 23
Views: 9916

Re: Double images

A simple test is to use a working pair of binoculars. If you can observe properly with them using both eyes it should be possible to observe with a binocular microscope. There are quite a few people who can't do this. For some it might be a good compromise to use a camera and monitor to do microscop...
by MicroBob
Fri Jan 08, 2021 9:24 pm
Forum: Specimens, samples and slides
Topic: Radiolaria material in block form - how to break it up!
Replies: 3
Views: 173

Radiolaria material in block form - how to break it up!

Hi Michael and all, here we can collect information, thoughts and experiences on the breaking up of radiolaria material that is available in block form. I once was promised some radiolaria material in exchange for japanese star sand. What arrived was looking like a little piece of school chalk that ...
by MicroBob
Fri Jan 08, 2021 8:52 pm
Forum: Beginner's corner
Topic: What magnification does AmScope MD130 1.3MP camera have?
Replies: 10
Views: 526

Re: What magnification does AmScope MD130 1.3MP camera have?

Hi Michael,
I wouldn't have expected that somebody would point out this defect as a feature! :lol:
A fairly simple achromatic lens should be able to rectify this problem.

Bob