Search found 1975 matches

by MicroBob
Wed May 06, 2020 1:14 pm
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1425

Re: Greetings from Frankfurt

jemarch wrote:
Wed May 06, 2020 12:02 pm
Interestingly Zeiss manuals are adamant in that the right position of the condenser is _always_ at the top position...
This is true - as long as you use the right condenser for the objective. :D There are special low power condensers for low power objectives.
by MicroBob
Wed May 06, 2020 10:29 am
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1425

Re: Greetings from Frankfurt

Hi, for optics cleaning "Wundbenzin" and "Augenwatte" are recommended, when blowing off the dust and wiping with condensed breath don't work. One warning: Exactly these old Zeiss immersion oil bottles tend to break - so better fill the oil in a new bottle. Is it marked PCB-free? A dropper bottle is ...
by MicroBob
Mon May 04, 2020 5:45 am
Forum: Beginner's corner
Topic: WHAT CAN I DO????????
Replies: 9
Views: 594

Re: WHAT CAN I DO????????

Hi Shirley, the development of such a water sample can be quite different and you have some means to influence it. What is sure is that it will continue to develop so you won't just keep what you have. When the mixture of living plants and animals is right the sample can even survive alive in a clos...
by MicroBob
Sun May 03, 2020 9:25 am
Forum: Microscopy accessories
Topic: Canon M50 limitations
Replies: 21
Views: 940

Re: Canon M50 limitations

Bob, thanks very much for reminding me about the "silent mode" feature. It took some digging but I did find the feature on the M50 but it is only available when shooting in the so called "SCN" mode, in this mode the camera is set to "Auto" and there doesn't seem to be any way to regain control. The...
by MicroBob
Sat May 02, 2020 9:08 pm
Forum: Microscopy accessories
Topic: Canon M50 limitations
Replies: 21
Views: 940

Re: Canon M50 limitations

Hi Mike,
you should use the mirror lock up anyway. Otherwise the image quality would be really limited. The question is whether the shutter closes and reopens from live view befor exposure. Some Canons are clean here, some don't. This would be my most important feature.

Bob
by MicroBob
Sat May 02, 2020 8:18 pm
Forum: Camera systems and imaging
Topic: Raspberry Pi High Quality Camera
Replies: 14
Views: 822

Re: Raspberry Pi High Quality Camera

I my view there is a lot of potential in combining simple optics with intelligent computer control. A Planapo 16x costs several thouthands of €. At the same time automatic stitching and stacking with a simple 100:1 achromat for 60€ could offer even more image resolution over an even wider field. So ...
by MicroBob
Sat May 02, 2020 8:04 pm
Forum: Microscopes and optics
Topic: Old Scope Who is the Maker?
Replies: 12
Views: 726

Re: Old Scope Who is the Maker?

This is how Will microscopes looked like: https://www.ebay.de/sch/i.html?_from=R40&_trksid=p2047675.m570.l1313.TR12.TRC2.A0.H0.Xwill+mikroskop.TRS0&_nkw=will+mikroskop&_sacat=0 Your microscope is neither from Will/Hund or Leitz. A good indicator are the used screws: Do they look like those in a germ...
by MicroBob
Sat May 02, 2020 7:53 pm
Forum: Illumination Techniques
Topic: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp
Replies: 22
Views: 1169

Re: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp

Hi Don, don't worry, the guys here just want to help. It looks as if there might be a simple way to improve the quality of your images. Exposure, image editing and image compression can have a strong effect on the output. Generally microscope photos don't need a lot of pixels, so while 640x480 are n...
by MicroBob
Sat May 02, 2020 2:07 pm
Forum: Microscopes and optics
Topic: Old Scope Who is the Maker?
Replies: 12
Views: 726

Re: Old Scope Who is the Maker?

Hi Alan, I think this was a typical oem-brand microscope that was sold under names like Novex, Euromex, Jungner, Phywe, BMS etc. . When you google for these names you will probably find images of similar models. I can imagine that they are even still sold somewhere. It is not from Will like the obje...
by MicroBob
Sat May 02, 2020 6:28 am
Forum: Camera systems and imaging
Topic: Raspberry Pi High Quality Camera
Replies: 14
Views: 822

Re: Raspberry Pi High Quality Camera

This is an interesting development! I use a c-mount camera with an old Pentax 110 SLR-Lens to connect directly to an eyepiece which works fine. The SOny 1/2,3'' chip in this camera could be similar to the one in the Pentax Q. This camera wasn't quite as good as an APS-C camera but good enough for ma...
by MicroBob
Fri May 01, 2020 7:36 pm
Forum: Pictures and Videos
Topic: Micro hedgehog
Replies: 14
Views: 2186

Re: Micro hedgehog

I have a couple of hedgehog spines somewhere from an overrun hedgehog scraped from the street. They also have quite different hairs, the ones at the seam between belly fur and spines are a mixture between hairs and spines, spikey stiff hairs. Right now he plays civil servant mikado with us: The one ...
by MicroBob
Fri May 01, 2020 3:24 pm
Forum: Pictures and Videos
Topic: Micro hedgehog
Replies: 14
Views: 2186

Re: Micro hedgehog

Hi together, since our micro-hedgehog has not jet prooved willing to wake up we have dug him out of his kitchen tissue and newspaper nest and not he is awaking inside. Though he is not a year old he behaves like a human teenager - stay in bed whatever happens! He is looking well and has slowly start...
by MicroBob
Fri May 01, 2020 3:19 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

Hi together,
nice to work on a common topic together!

When I look at the images of pollen I often wonder how it is possible to be not allergic against them. It can't be agreeable to get these into the eye!

Bob
by MicroBob
Thu Apr 30, 2020 6:45 pm
Forum: Pictures and Videos
Topic: Since we're doing some firsts, here is my first photo stack using Enfuse.
Replies: 9
Views: 588

Re: Since we're doing some firsts, here is my first photo stack using Enfuse.

I had a short look at enfuse and my impression was that it is a stitching and HDR software, not a focus stacking software - but I might have overseen this feature. For focus stacking I use Picolay, out of habit and because it is free. The developer is fine tuning the program all the time so one can ...
by MicroBob
Wed Apr 29, 2020 5:56 am
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1425

Re: Greetings from Frankfurt

Would you suggest that the PZO DIC could work on the Zeiss GFL ? Hi Doron, The GFL and Junior look very similar but are designed completely different. The GFL already has the Zeiss Standard ring dovetail into which the condenser is inserted from above. The Junior (and the PZO MB30 and Biolar) has a...
by MicroBob
Tue Apr 28, 2020 3:41 pm
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1425

Re: Greetings from Frankfurt

You mean it won't work for practical purposes? :/ My goal was to buy a simple, yet high quality and durable microscope with which to learn the basic technique. Hi, this microscope will suit you just fine. The Zeiss Standard Junior is a high quality and quite modern microscope that really is a joy t...
by MicroBob
Sun Apr 26, 2020 6:47 pm
Forum: Illumination Techniques
Topic: Interesting DIC-like effect
Replies: 5
Views: 868

Re: Interesting DIC-like effect

Hi Chris, I would call this circular oblique lighting, COL. It offers a nice combination of contrast and resolution but is a bit fiddly to set up and introduces artifacts. I think the true resolution gain comes for the use of the advantageous outer rays of the illumination light cone. I just saw tha...
by MicroBob
Fri Apr 24, 2020 9:37 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

mrsonchus wrote:
Thu Apr 23, 2020 9:37 pm
No more pollen images - I promise! :D :D :D
Please don't keep this promise! You las post was again very interesting for me especially since it covered som any other aspects.

Here a stacked image made with the Zeiss 40:1 0,85 oil achromat in DIC:

Bob
by MicroBob
Thu Apr 23, 2020 5:35 pm
Forum: Pictures and Videos
Topic: Amphipleura pellucida
Replies: 10
Views: 784

Re: Amphipleura pellucida

Hi Glen, great image! This is really a difficult diatom. Here in Germany is a pond where they can be found all year round: Kraterquelle in Bad Nenndorf. I was sent a bottle of water and made some slides. Here the best I got at this time, not nearly as good as your image! I used a decentered dark fie...
by MicroBob
Thu Apr 23, 2020 12:07 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

A couple more single bright field images, Leitz NPL Fluotar 50:1 1,0 and Leitz Fluorescenz 63:1 1,3.
by MicroBob
Thu Apr 23, 2020 9:13 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

Hi Daroush,
I did a bit of image editing on your bright field image - how do you like it?
And a single bright field image from my slide.

Bob
by MicroBob
Thu Apr 23, 2020 1:40 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

Hi Daruosh,
the visibility of my pollen came mainly from the yellow original colour and the pink fuchsin stain. Did you use fuchsin? It seems to attatch well to the pollen. I added a tiny amount to my glycerin jelly until it looked like John's.

Bob
by MicroBob
Wed Apr 22, 2020 3:52 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

Hi John, thank you for the explanations! I mixed a glycerin jelly like this: 0,7g ordinary household gelatine 4,2g dem. water 5,0g glycerin 0,01g thymol a trace of fuchsin and mixed it on my heater/stirrer. It became a solid jelly after cooling down. Making slides was easy and the results are really...
by MicroBob
Wed Apr 22, 2020 5:56 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3189

Re: Pollen Fine Detail

Hi John,
you've got some really impressive results here! These are probably the best pollen images I have seen so far.
What I didn't understand is how you applied the fuchsine - is this a two step process: First pollen into stained jelly, then into clear jelly?

Bob
by MicroBob
Tue Apr 21, 2020 7:27 pm
Forum: Pictures and Videos
Topic: Moth and swiss chard seeds
Replies: 5
Views: 426

Re: Moth and swiss chard seeds

Nice images of interesting objects!
by MicroBob
Tue Apr 21, 2020 9:39 am
Forum: Introduce yourself
Topic: Hello everyone.
Replies: 6
Views: 610

Re: Hello everyone.

Hi Zohar, welcome to the forum! The MKB website really is worth the translation hassle, you won't find a better source of information on botanic sectioning. DeepL is said to translate even better. Here is a quite comprehensive list of botanic sectioning threads in the german forum: https://www.mikro...
by MicroBob
Mon Apr 20, 2020 8:20 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 776

Re: Imaging with different coverglass thickness

Hi Andrew, apart from the cover slip thickness the further medium between cover slip and object is part of the calculation.If there is a tiny water layer cover slips of less than 0,17mm might actually offer a better image. Apart from that: a #1 is thinner than a #1,5! So if you can't focus the probl...
by MicroBob
Mon Apr 20, 2020 7:07 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 772

Re: More phase contrast photos of cleaned diatoms

I've watched a video on YouTube showing how to clean and mount the diatoms, but I cannot reproduce the results shown in the same video. Hi Daruosh, I have tried his method and was quite happy with the results. Here in Germany "bleach" is sold under the name "Clorix" and contains 2,6% sodiumhypochlo...
by MicroBob
Mon Apr 20, 2020 2:09 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 772

Re: More phase contrast photos of cleaned diatoms

Hi together, a nice set of images again, Doron! Green: I frequently divide the image apart ito th RGB channels when I only need a b/w image. I usually end up with the green channel. Blue is usually quite similar and also quite good, red is quite different in image ans usually a lot worse. But this i...
by MicroBob
Sun Apr 19, 2020 8:18 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 737

Re: Stomata in tulip leaf

Another simple way to observe stomata: Hand sections! By omitting the microtome one gains flexibility and speed. Aim is not a planparallel section but a wedge that at least has the right thickness in an area. For thin objects it is feasible to try to cut planparallel and advance in tiny increments. ...