Search found 1986 matches

by MicroBob
Thu Apr 23, 2020 12:07 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3350

Re: Pollen Fine Detail

A couple more single bright field images, Leitz NPL Fluotar 50:1 1,0 and Leitz Fluorescenz 63:1 1,3.
by MicroBob
Thu Apr 23, 2020 9:13 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3350

Re: Pollen Fine Detail

Hi Daroush,
I did a bit of image editing on your bright field image - how do you like it?
And a single bright field image from my slide.

Bob
by MicroBob
Thu Apr 23, 2020 1:40 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3350

Re: Pollen Fine Detail

Hi Daruosh,
the visibility of my pollen came mainly from the yellow original colour and the pink fuchsin stain. Did you use fuchsin? It seems to attatch well to the pollen. I added a tiny amount to my glycerin jelly until it looked like John's.

Bob
by MicroBob
Wed Apr 22, 2020 3:52 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3350

Re: Pollen Fine Detail

Hi John, thank you for the explanations! I mixed a glycerin jelly like this: 0,7g ordinary household gelatine 4,2g dem. water 5,0g glycerin 0,01g thymol a trace of fuchsin and mixed it on my heater/stirrer. It became a solid jelly after cooling down. Making slides was easy and the results are really...
by MicroBob
Wed Apr 22, 2020 5:56 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3350

Re: Pollen Fine Detail

Hi John,
you've got some really impressive results here! These are probably the best pollen images I have seen so far.
What I didn't understand is how you applied the fuchsine - is this a two step process: First pollen into stained jelly, then into clear jelly?

Bob
by MicroBob
Tue Apr 21, 2020 7:27 pm
Forum: Pictures and Videos
Topic: Moth and swiss chard seeds
Replies: 5
Views: 447

Re: Moth and swiss chard seeds

Nice images of interesting objects!
by MicroBob
Tue Apr 21, 2020 9:39 am
Forum: Introduce yourself
Topic: Hello everyone.
Replies: 6
Views: 639

Re: Hello everyone.

Hi Zohar, welcome to the forum! The MKB website really is worth the translation hassle, you won't find a better source of information on botanic sectioning. DeepL is said to translate even better. Here is a quite comprehensive list of botanic sectioning threads in the german forum: https://www.mikro...
by MicroBob
Mon Apr 20, 2020 8:20 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 789

Re: Imaging with different coverglass thickness

Hi Andrew, apart from the cover slip thickness the further medium between cover slip and object is part of the calculation.If there is a tiny water layer cover slips of less than 0,17mm might actually offer a better image. Apart from that: a #1 is thinner than a #1,5! So if you can't focus the probl...
by MicroBob
Mon Apr 20, 2020 7:07 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 791

Re: More phase contrast photos of cleaned diatoms

I've watched a video on YouTube showing how to clean and mount the diatoms, but I cannot reproduce the results shown in the same video. Hi Daruosh, I have tried his method and was quite happy with the results. Here in Germany "bleach" is sold under the name "Clorix" and contains 2,6% sodiumhypochlo...
by MicroBob
Mon Apr 20, 2020 2:09 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 791

Re: More phase contrast photos of cleaned diatoms

Hi together, a nice set of images again, Doron! Green: I frequently divide the image apart ito th RGB channels when I only need a b/w image. I usually end up with the green channel. Blue is usually quite similar and also quite good, red is quite different in image ans usually a lot worse. But this i...
by MicroBob
Sun Apr 19, 2020 8:18 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Re: Stomata in tulip leaf

Another simple way to observe stomata: Hand sections! By omitting the microtome one gains flexibility and speed. Aim is not a planparallel section but a wedge that at least has the right thickness in an area. For thin objects it is feasible to try to cut planparallel and advance in tiny increments. ...
by MicroBob
Sun Apr 19, 2020 4:08 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Re: Stomata in tulip leaf

Here a rough comparison of images taken with different contrast techniques and little image editing, all single shots. On cause there is a lot of room for improvement in every shot, it just shows round about how much potential is there.

Bob
by MicroBob
Sun Apr 19, 2020 10:59 am
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Re: Stomata in tulip leaf

Hi Daroush, I would just try what you have. A semi-transparent glue might be just perfect as you get an amplitude object from it. One useful property would be good adherance to hard surfaces, this should be given in a glue that is intended for mounting electronic components. The glue should have a s...
by MicroBob
Sun Apr 19, 2020 10:03 am
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Re: Stomata in tulip leaf

To show that no expensive DIC is necessary here a single shot with oblique lighting.
by MicroBob
Sun Apr 19, 2020 9:01 am
Forum: Beginner's corner
Topic: What to buy. Advice
Replies: 3
Views: 358

Re: What to buy. Advice

You have observed right: Usually stereo microscopes have barely the needed resolution for acceptable visual oberservation, less than a normal microscope objective offers. This has to do with the aim to offer a good working distance with not too wide and expensive optics. At 40x combined mag a typica...
by MicroBob
Sat Apr 18, 2020 11:54 pm
Forum: Miscellaneous
Topic: Slides
Replies: 5
Views: 513

Re: Slides

A condenser that is not intended for oil immersion might be better as it usually has more distance to the slide. Like a n.a. 0,6 or 0,9 condenser.
by MicroBob
Sat Apr 18, 2020 8:19 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Re: Stomata in tulip leaf

Well, this is how I came to UV hardening nail polish: I used to teach commercial apprentices some technical stuff at work, each one a month. One morning I came into my office and my apprentice of the month held her hands in my direction and said "I did this myself!". My interest was awakened: "New D...
by MicroBob
Sat Apr 18, 2020 5:59 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 750

Stomata in tulip leaf

Hi together, I'm preparing a video conference meeting on stomata and did a couple of nail polish impressions and hand sections today. Here is a photo of an impression in UV hardening nail polish of the underside of a tulip leaf. I put a tiny droplet of the nail polish on the leaf and then press a 18...
by MicroBob
Sat Apr 18, 2020 2:19 pm
Forum: My microscope
Topic: Zeiss Photomicroscope I
Replies: 19
Views: 1001

Re: Zeiss Photomicroscope I

@Charles: Nice to see new posts coming from you, it has been a while!
by MicroBob
Sat Apr 18, 2020 2:18 pm
Forum: My microscope
Topic: Zeiss Photomicroscope I
Replies: 19
Views: 1001

Re: Zeiss Photomicroscope I

The nosepiece bayonett was changed in 1961. The older Phomi 1 model had the white camera release button in the base in front of the field aperture. It is much better to have the newer one as some accessories for the older one are difficult to find. At first this microscope is a bit intimidation, but...
by MicroBob
Sat Apr 18, 2020 9:19 am
Forum: Miscellaneous
Topic: Slides
Replies: 5
Views: 513

Re: Slides

The slide thickness is part of the condenser design parameters. A 2mm slide will work to some degree but illumination couldn't be set up as intended.
Are slides difficult to get for you?
by MicroBob
Sat Apr 18, 2020 7:00 am
Forum: Microscopes and optics
Topic: LOMO as a brand
Replies: 25
Views: 962

Re: LOMO as a brand

Here in Germany we have a lot of simple LOMO Biolams on the used market. As far as I know they were not sold in former East Germany (DDR) but inly in West Germany over a mail order campany and later by Bresser. The more advanced LOMO microscopes are very rare here. I have one, that was painted black...
by MicroBob
Fri Apr 17, 2020 7:35 pm
Forum: Microscopes and optics
Topic: High-Powered Objective always hits the coverglass
Replies: 5
Views: 402

Re: High-Powered Objective always hits the coverglass

The concave slides are not useful for high power work: The lower objects are to far away from the cover slip top for the correction of the objective. The concave surface also forms a kind of lens that tends to upset the image quality. But for low power observation of bigger water life they are very ...
by MicroBob
Fri Apr 17, 2020 6:17 pm
Forum: Microscopes and optics
Topic: High-Powered Objective always hits the coverglass
Replies: 5
Views: 402

Re: High-Powered Objective always hits the coverglass

Hi Don, stronger objectives have small free working distances. Your cover slips should not exceed 0,17mm by much. I once bought chinese ones that were up to 0,25mm! But most probably your preparations are not as thin as they should be. When you mount a wedged section part of the object will be too f...
by MicroBob
Fri Apr 17, 2020 8:33 am
Forum: Beginner's corner
Topic: PLAN APO vs APO
Replies: 8
Views: 490

Re: PLAN APO vs APO

Are you using the right eyepiece for the objective?
by MicroBob
Fri Apr 17, 2020 5:25 am
Forum: Beginner's corner
Topic: PLAN APO vs APO
Replies: 8
Views: 490

Re: PLAN APO vs APO

I think PLAN objectives were increasingly devoloped from 1960 on. The first apochromat was made in 1883, the first planapo in 1938. A planapo is a lot more difficult to make than a apo. The old 33mm Zeiss apos gave a very nice image but had a lot of field curvature.
by MicroBob
Fri Apr 17, 2020 5:19 am
Forum: Microscopes and optics
Topic: Anyone familiar with a Steindorff Microscope?
Replies: 5
Views: 269

Re: Anyone familiar with a Steindorff Microscope?

That is true.
I think with the microbe hunter they tried to present themselves as a research microscope brand in the USA. I'm not aware that they had higher corrected optics of special contrast methods in their lineup. The microbe hunter was more or less a magnificiently looking dummy.
by MicroBob
Fri Apr 17, 2020 5:13 am
Forum: Microscopes and optics
Topic: Highest power LED suitable for microscope?
Replies: 15
Views: 702

Re: Highest power LED suitable for microscope?

Hi Pete,
the Cree XHP 50 and 70 of 18 and 32 W could be an option. White LEDs generally have a dent in the spectrum around 500nm. This is less severe on warm white models and they can be used with a light blue filter. A 4000K-5000K LED gives a nice neutral tone in my eyes.

Bob
by MicroBob
Thu Apr 16, 2020 7:32 pm
Forum: Microscopes and optics
Topic: Anyone familiar with a Steindorff Microscope?
Replies: 5
Views: 269

Re: Anyone familiar with a Steindorff Microscope?

I second Scarodactyls impression that this is no Steindorff microscope. On the other hand side: The real Steindorff microscopes from Berlin were not too hot too. Mechanically robust but optically very simple. They were sold to doctor's offices for simple routine tasks and nothing the amateur (doing ...
by MicroBob
Thu Apr 16, 2020 7:28 pm
Forum: Microscopes and optics
Topic: LOMO as a brand
Replies: 25
Views: 962

Re: LOMO as a brand

Long time ago I used LOMO MBC10 stereo microscopes Hi Riku, this is unlikely! :D The MBS 10 was actually made by the russian company Lytkarino, not LOMO. But this is mixed up very often. I have used an MBS 10 a few times and liked it in the lower and medium powers, not so much in the higher magnifi...