Search found 2268 matches

by MicroBob
Fri Oct 16, 2020 12:26 am
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 24
Views: 1184

Re: Please help a microscope noob with objectives

The microscope seems to be well designed and the results are good. For a microscopist the question would be: "What do I gain and loose by using a 3D-printed microscope over using a normal lab microscope as my basis?". A lab microscope for use in this project could be very cheap as expensive things l...
by MicroBob
Fri Oct 16, 2020 12:12 am
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 24
Views: 1184

Re: Please help a microscope noob with objectives

On my android phone I use snapseed for image editing and sizing down. The suffix has to be jpg not jpeg in some forums. I will have a look at you google album!
by MicroBob
Thu Oct 15, 2020 10:19 pm
Forum: Microscopes and optics
Topic: Please help a microscope noob with objectives
Replies: 24
Views: 1184

Re: Please help a microscope noob with objectives

Hi Bogdan,
pictures have to be max. 1024 pixel long side. Videos can't be uploaded here but you can post a link to an external video like on vimeo or youtube.

I see much future in projects like this so I would be happy to see how far you got so far.

Bob
by MicroBob
Wed Oct 14, 2020 6:15 pm
Forum: Specimens, samples and slides
Topic: Safety and Legality of Prepared Pathology Slides
Replies: 9
Views: 1012

Re: Safety and Legality of Prepared Pathology Slides

Usually people who have access to these bacteria know how to make sterile slides from them and do so. On the other hand side errors happen: A guy told me once that he pushed a trolley full of dangerous samples once around a university after being told that they had been in the autoclave - only they ...
by MicroBob
Mon Oct 12, 2020 8:36 am
Forum: Microscopes and optics
Topic: Are Zeiss Fluorescence filters interchangeable?
Replies: 2
Views: 190

Re: Are Zeiss Fluorescence filters interchangeable?

Hi Mitchell, this company used to sell interference filter sets: https://www.ebay.de/sch/jenmedgmbh/m.html?_nkw=&_armrs=1&_ipg=&_from= I bought 8 for 45€ incl. shipping. They are a bit smaller in diameter than those fpr the III RS but can be fitted and work well for me. Perhaps they have more in sto...
by MicroBob
Sat Oct 10, 2020 5:49 pm
Forum: Specimens, samples and slides
Topic: Diatom Mounting Medium Hunt
Replies: 21
Views: 3698

Re: Diatom Mounting Medium Hunt

I have tapped a big American styrax tree and it is very slowly producing resin. Should have a good crop in the spring I'll check back if I get enough to distribute Hi Bram, I didn't have Styrax very bright on my radar. I would be interested to hear what you are doing and how you are attempting to m...
by MicroBob
Fri Oct 09, 2020 4:18 pm
Forum: Resources (online, books etc.)
Topic: Marine diatom and radiolaria identification book as download
Replies: 5
Views: 718

Marine diatom and radiolaria identification book as download

Hi together, imagine a pandemic stikes and you have to spend more time with yourself or do home office peering through the microscope. You might appreciate a couple of pages on indentification of radiolaria and diatom of cause! Here is a book of over 600 pages that will probaly be sufficient even fo...
by MicroBob
Thu Oct 08, 2020 8:34 am
Forum: Specimens, samples and slides
Topic: Cover Slip Issues with Euparal
Replies: 10
Views: 830

Re: Cover Slip Issues with Euparal

Hi Michael, after a recommendation by an experienced microscopist I mixed Hoyers mix from Gutenbergs glue but haven't used it so far. Problems with the quality of the available Gummi arabicum are well known. Hoyer's medium is generally only seen as semi-permanent, in many (but not all, would be too ...
by MicroBob
Wed Oct 07, 2020 5:49 pm
Forum: Microscopes and optics
Topic: Probably dumb question
Replies: 12
Views: 794

Re: Probably dumb question

Hi Ross, since this is not a thread that transmits power or moves a lot no real lubrication is necessary. The material don't tend to attatch to another under normal conditions. A fine smear of sewing machine oil can't harm though. I do have a few microscope objectives and never had the impression th...
by MicroBob
Wed Oct 07, 2020 8:12 am
Forum: Specimens, samples and slides
Topic: Cover Slip Issues with Euparal
Replies: 10
Views: 830

Re: Cover Slip Issues with Euparal

Euparal contains a lot of solvent that gasses out sooner or later. So when you prepare a nice slide of a sturdy thicker specimen the solvent will evaporate and air pockets will move under the cover slip, finally taking up half the area. You can counter this by adding more Euparal and then even more ...
by MicroBob
Tue Oct 06, 2020 8:37 pm
Forum: Microscopes and optics
Topic: Help disassembling a Zeiss objective
Replies: 13
Views: 485

Re: Help disassembling a Zeiss objective

Hi Bram, I can't help you but my impression is that incident lighting objecties are more often severely damaged than transmitted light objectives. I have seen dents, scratches and glass corrosion that are not often seen on transm. objectives. This may be the result of rough workshop use and use of c...
by MicroBob
Sun Oct 04, 2020 4:23 pm
Forum: Home-made microscope adaptations
Topic: DIY plan objectives
Replies: 7
Views: 732

Re: DIY plan objectives

Hi Patta,
very nice design and great looking threads!
It would be nice to see more of the performance of these lenses and how they do in practical use.

Bob
by MicroBob
Sun Oct 04, 2020 4:20 pm
Forum: My microscope
Topic: My microscope (overkill eyepiece alert!)
Replies: 22
Views: 1800

Re: My microscope (overkill eyepiece alert!)

Hi Javier, the tube lenght and the placing of the intermediate image in the tube are part of the objective's calculation. So using other eyepieces can have an impact on the image quality. Whether this really shows in practical use is another question, it is best to try yourself. By swapping back and...
by MicroBob
Fri Oct 02, 2020 9:51 am
Forum: Beginner's corner
Topic: What Is This Item For.
Replies: 5
Views: 911

Re: What Is This Item For.

Hi Stephen,
you probably can use it to adjust the focus drive. On your second photo, the focus gear shaft is thicker on the left side. There is probably a hole in it where you can apply this pin wrench.

Bob
by MicroBob
Mon Sep 21, 2020 8:32 am
Forum: Beginner's corner
Topic: What can I do with these stains?
Replies: 2
Views: 259

Re: What can I do with these stains?

Hi, nice to hear that you found an interest in the microscopic world! Here is a database where you will find a possible applications and a lot of information on your stains: http://aeisner.de/ You might have to stress Google translator or DeepL a bit. Generally it is a good idea to keep in mind that...
by MicroBob
Wed Sep 16, 2020 6:46 am
Forum: Digital processing
Topic: Altami studio price anyone?
Replies: 11
Views: 1214

Re: Altami studio price anyone?

Gasman wrote:
Tue Sep 15, 2020 9:57 pm
she(he?) was a regular.
regularly promoting this product, nothing else.

I wouldn't be astonished it this were some kind of spy software...
by MicroBob
Tue Sep 15, 2020 8:57 pm
Forum: Digital processing
Topic: Altami studio price anyone?
Replies: 11
Views: 1214

Re: Altami studio price anyone?

Hi Steve, there was no one recommending Altami studio in this forum, but user amanda1 tried to do some product placement here for a while: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=25&t=5656&p=50989&hilit=altami#p50989 I wouldn't use a software that is advertised like this, if it...
by MicroBob
Tue Sep 15, 2020 6:24 am
Forum: Illumination Techniques
Topic: Do the different oblique filters really do anything different?
Replies: 6
Views: 633

Re: Do the different oblique filters really do anything different?

Hi Jerradin, oblique lighting provides an asymmetric hollow light cone that leads to more contrast and resolution. The effect depends on the direcion, so you might have to turn the stop for the best effect. At first you should set up the bright field light so that the back lens of the objective is f...
by MicroBob
Sun Sep 13, 2020 9:10 pm
Forum: Specimens, samples and slides
Topic: Does anyone tried metal washer and flat rubber o-ring as spacer?
Replies: 9
Views: 1646

Re: Does anyone tried metal washer and flat rubber o-ring as spacer?

Hi together, as long as the specimen is close to the cover slip it will be fine for the objective. But what about the condenser? It is calculated for usual slide thickness and won't work offer optimal illumination when you increase the distance between condenser front lens and specimen this much. A ...
by MicroBob
Sun Sep 13, 2020 12:02 pm
Forum: Specimens, samples and slides
Topic: Prepared slide storage
Replies: 7
Views: 1507

Re: Prepared slide storage

Hi Kirby, the drying mountant at the edges has enough tack to hold the cover slip in place when the slide is hanging horizontal and nothing contacts the cover slip. The mountant layer can easily be thick enough to make it impossible to focus on the specimen when it sinks down to the slide. The addit...
by MicroBob
Sat Sep 12, 2020 6:37 am
Forum: Specimens, samples and slides
Topic: Prepared slide storage
Replies: 7
Views: 1507

Re: Prepared slide storage

Hi Tony,
in my view it is best to store slides flat but cover slip down. If the mountant is not fully hardened this prevents that the specimen moves away from the cover slip and sinks down to the slide. For mountants that harden dependably this is not necessary of cause.

Bob
by MicroBob
Fri Sep 11, 2020 5:06 pm
Forum: Microscopy accessories
Topic: Info needed on AO 820 "tome"
Replies: 7
Views: 1207

Re: Info needed on AO 820 "tome"

Razor blades are fairly thin for sectioning and will probably chatter quicker than comfortable, so I wouldn't spend much energy on getting such a holder.
Sharp, a lot thicker and very sharp are cutter blades by the company OLFA, e.g. LBB 50.
by MicroBob
Thu Sep 10, 2020 2:26 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 6120

Re: Diatom selection experience

Hi Doron, for your single diatom mouts I had another idea: You might set the diatom in a tiny drop of Pleurax on a cover slip and cure it. Then mount the cover slip on the slide with a different mountant that doesn't need a bubbling curing action, like an UV glue. This way you would be sure that the...
by MicroBob
Thu Sep 10, 2020 5:50 am
Forum: Specimens, samples and slides
Topic: Cargill immersion oil "B" probably 1960s
Replies: 17
Views: 2914

Re: Cargill immersion oil "B" probably 1960s

Hi together, just to complete the topic: Cargille didn't respond to my friendly e-Mail so I have to expect that the oil contains at least PCB, possibly other harmful substances the didn't want to discover. I brought the oil to our local recycling center, with an added sticker "contains PCB". I would...
by MicroBob
Wed Sep 09, 2020 7:39 pm
Forum: Microscopes and optics
Topic: Brass Rack and pinions designed to run dry?
Replies: 14
Views: 1719

Re: Brass Rack and pinions designed to run dry?

Hi together, the combing action of these gears involves a sliding action and doesn't hold up well without lubrication. Brass (copper/zinc) is not as nice as bronze (copper/tin) as a friction partner. Stripped gears are often the result of dropped instruments or of forcing gummed up movements (move, ...
by MicroBob
Wed Sep 09, 2020 7:26 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 6120

Re: Diatom selection experience

Hi Doron, nice to hear of you progress! According to your note 2: Michel Haak suggests polyacrylamide, not polyamide. I'm no chemist and don't know how far this is apart. http://www.diatoms.eu/de/node/77 The high curing temperature of Pleurax really narrows down the number of possible candidates for...
by MicroBob
Tue Sep 08, 2020 5:36 pm
Forum: Beginner's corner
Topic: Microscope for engine oil analysis
Replies: 43
Views: 2927

Re: Microscope for engine oil analysis

Hi Pete, the graph shows the analysis of a silver brazed steel part, I made for a group meeting on the material steel and materials science methods and had these graphs made at our university. I like measuring instruments a lot and would enjoy to have a SEM with EDAX scanner, but this is just overki...
by MicroBob
Sat Sep 05, 2020 5:58 pm
Forum: Microscopes and optics
Topic: Microscope Maintenance
Replies: 25
Views: 2452

Re: Microscope Maintenance

Professionals have a tendency to cut corners when it improves their income. So I would expect that they would be willing to use a powerful cleaner, even if there are gentler methods available. But even the careful amateur sometimes has to use rougher methods when nothing else helps, like with mouldy...
by MicroBob
Sat Sep 05, 2020 3:07 pm
Forum: Microscopes and optics
Topic: Microscope Maintenance
Replies: 25
Views: 2452

Re: Microscope Maintenance

Hi together, the safety data sheets often are an interesting source of information. Apparently Lens Clens 1 consists of these dangerous components: Solvent content: Organic solvents:46.0 % Water:45.9 % VOC content:46.0 % Solids content:2.8 % CAS: 67-63-0RTECS: NT 8050000Isopropyl alcohol Flam. Liq. ...
by MicroBob
Sat Sep 05, 2020 7:33 am
Forum: Beginner's corner
Topic: Keeping a 60x objective from crashing
Replies: 10
Views: 1728

Re: Keeping a 60x objective from crashing

Hi Jerradin, this would be an easy test: -Take a slide, mark it with a blue waterproof pen -Take the cover slip you had problems with (or any other), makr the underside with a black waterproof pen -Mount with drop of water -Can you focus on both marks with the 60:1? -Adjust water layer and try again...