Search found 2394 matches

by MicroBob
Fri Dec 18, 2020 7:52 pm
Forum: Beginner's corner
Topic: Rings on Ants
Replies: 4
Views: 635

Re: Rings on Ants

Hi Andreas,
I have asked the same question about a bubble inside a wood section not that long ago! :lol:

Bob
by MicroBob
Fri Dec 18, 2020 10:19 am
Forum: Beginner's corner
Topic: Rings on Ants
Replies: 4
Views: 635

Re: Rings on Ants

Hi,
these are gas bubbles in the water, perhaps coming from inside the ant. When you press on the cover slip while observing they will move.

Bob
by MicroBob
Thu Dec 17, 2020 4:33 pm
Forum: Microscopes and optics
Topic: Use of oil immersion objective with live samples
Replies: 8
Views: 1014

Re: Use of oil immersion objective with live samples

Hi Tim, this can be done without much problem when considering a couple of things: - Use a fairly big cover slip, e.g. 20x20 - For the standard biological microscope use the thinner viscosity of immersion oils, not the thicker flowing one for inverted microscopes - Use thin water layer so avoid sand...
by MicroBob
Wed Dec 16, 2020 9:25 am
Forum: Microscopes and optics
Topic: Zeiss 465285 Inko Condenser
Replies: 8
Views: 846

Re: Zeiss 465285 Inko Condenser

Hi Doron, of cause it is not ideal to put optical parts under stress, thermal or mechanical. But I think these lenses can reach 60°C when used with a powerful halogen lamp in warmer environment, so 60°C should be barely acceptable. I don't actually know whether there a cemented lens groups in these ...
by MicroBob
Wed Dec 16, 2020 7:07 am
Forum: Microscopes and optics
Topic: Zeiss 465285 Inko Condenser
Replies: 8
Views: 846

Re: Zeiss 465285 Inko Condenser

The n.a. 1.4 top lens usually unscrews from the 0,32 lens. It probably is stuck from gummed up immersion oil. Heating the lenses to 60°C for a while might soften the bond so it can be unscrewed.
by MicroBob
Tue Dec 15, 2020 9:09 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

Hi John, thank you very much for the detailed explanations! I haven't looked closer at my staining results for many years - it became colourful - good! :oops: While preparing my part of our last group meeting I took the time to do some more experimenting with botanical stains and made a little progr...
by MicroBob
Tue Dec 15, 2020 7:53 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

Hi John,
your images make me think to have a closer look into fungus infested plants! It shouldn't be too difficult to find suitable plant material, I would perhaps take a closer look at our fruit trees.
What stains did you use for the last 2 images?

Bob
by MicroBob
Mon Dec 14, 2020 9:50 pm
Forum: Beginner's corner
Topic: Stacked pictures come out Cartoonish
Replies: 15
Views: 1530

Re: Stacked pictures come out Cartoonish

does not offer a lot of quality. Well, at least the images are tiny! 8-) I'm no big stacking expert but what suprised me was that stacking doesn't necessarily lead to better images. In case of too few images I got stange looking and unsharp results. In case of objects with contrasty structures in m...
by MicroBob
Mon Dec 14, 2020 6:25 pm
Forum: Beginner's corner
Topic: Stacked pictures come out Cartoonish
Replies: 15
Views: 1530

Re: Stacked pictures come out Cartoonish

Can you post a single image for comparison?
As far as I remember you use a USB camera - right? These often don't offer the very best raw image quaöity due to small and low end sensors and counter this with heavy internal image corrections. That is not the best starting point for image stacking.
by MicroBob
Mon Dec 14, 2020 3:25 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

Hi John,
nice contrasty stain! Was this fungus in air or had it entered ito the tissue?

Bob
by MicroBob
Mon Dec 14, 2020 3:23 pm
Forum: My microscope
Topic: My Microscope is a converted vlogging cam-Idolcam
Replies: 9
Views: 1157

Re: My Microscope is a converted vlogging cam-Idolcam

Hi Jason,
I think you really forgot to mention that you are doing product placement here, since you are Idolcam in person.
Doing this openly is one thing but just entering the forum and pretending to be a macro photo newbie is an other thing - not my thing.

Bob
by MicroBob
Mon Dec 14, 2020 10:33 am
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

No, I just happened to read it here on page 18: https://www.waldeck-ms.de/wp-content/downloads/faerbemethoden_deutsch.pdf They try to sell us a ready mixed stain, but Eisner has the ingredients here: http://www.aeisner.de/methoden/farb118.html I have one Fuchsine, but there are at least three types,...
by MicroBob
Mon Dec 14, 2020 10:20 am
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

For Fungi Pianese stainig would work: Fungus pink, plant green.
by MicroBob
Mon Dec 14, 2020 7:45 am
Forum: My microscope
Topic: My Microscope is a converted vlogging cam-Idolcam
Replies: 9
Views: 1157

Re: My Microscope is a converted vlogging cam-Idolcam

Hi, welcome to this forum! Your images look good so camera and microscope objective seem to work together well. Your special approach might make it possible to work on topics outside the usual range. What type of camera is this and how do you like it? Can you do time lapse videos with it? Does it of...
by MicroBob
Sun Dec 13, 2020 8:30 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Re: Ilex leaf with worm hole - pen ink stain

Wes wrote:
Sun Dec 13, 2020 8:22 pm
Have you tried to section sick plants such as ones infected by fungi/bacteria?
No, this was just by accident. There are special stains to make fungi visible in plant tissue, perhaps for bacteria too. Might be a very attractive image of a destructive condition!
by MicroBob
Sun Dec 13, 2020 8:07 pm
Forum: Pictures and Videos
Topic: Ilex leaf with worm hole - pen ink stain
Replies: 11
Views: 967

Ilex leaf with worm hole - pen ink stain

Hi together, while preparing our last group meeting for this year I did some ilex leaf cross sections. On the leaf I saw tiny vore holes towards the central vein of the leaf. When doing experiments on staining processes I hit one of these worm holes and got a nice photo here. My idea is that the att...
by MicroBob
Fri Dec 11, 2020 12:00 pm
Forum: For forum members who want to buy and sell equipment
Topic: For sale: Blade holder for the hand / cylinder / table microtome
Replies: 9
Views: 3419

Re: For sale: Blade holder for the hand / cylinder / table microtome

Hi John, it actually is a pity that there are to my knowledge no really satisfying hand microtomes available new. This used to be a product that was made by companies like Jung, Leitz and Sartorius, probably not cheap but still affordable. Used with a cut-throat razor the investment was not worthwhi...
by MicroBob
Fri Dec 11, 2020 9:07 am
Forum: Specimens, samples and slides
Topic: [Hand microtome] Have you tried razor scraper?
Replies: 3
Views: 591

Re: [Hand microtome] Have you tried razor scraper?

Hi Kevin, I have developed a blade holder to use with a cylinder / hand microtome: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=16&t=7065&p=62601&hilit=blade+holder#p62601 The blades have to be exceptionally sharp and from the ones I compared the blades by OLFA were by far the ones ...
by MicroBob
Thu Dec 10, 2020 7:57 am
Forum: Specimens, samples and slides
Topic: Acquiring fluorescent stains/dyes
Replies: 5
Views: 991

Re: Acquiring fluorescent stains/dyes

You might have a look for one of these more common stains: Acridingelb Acridinorange Rhodamin-B Primulin gelb 4-Methyläsculetin Aesculin Euflavin Quercetin Chininsulfat The first 3 can be used to stain specific plant structures, the others may be little specific. Professional fluorescence stains can...
by MicroBob
Thu Dec 10, 2020 6:58 am
Forum: Microscopes and optics
Topic: New Stereo - Nikon SMZ800
Replies: 17
Views: 1586

Re: New Stereo - Nikon SMZ800

Hi John, congratulations to your new stereo microscope. Squeaking modern piece of equipment for me! I'm a bit cautious to look through instruments like this because I might not like my 1960s 1970s Leitz and Olympus anymore. :lol: I recently had a round stage insert and a rectangular stage insert wit...
by MicroBob
Wed Dec 09, 2020 2:11 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 810

Re: Experiments with botanic stains

Hi Doron,
I was looking for a black-orange stain, but start to prefer the blueish one too.
What I found is that bleaching the section with Clorix changes the apperance of the staining a lot.

Bob
by MicroBob
Wed Dec 09, 2020 8:02 am
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 746

Re: Question about setting up Zeiss DIC

Hi Wes, do you use the DIC system with the single analyser slide or the newer one with individual analyser slides pe robjective? The old system doesn't allow the use of the full objectives aperture anyway. Zeiss specifically sait to use oil immersion even for the low power objectives. This might be ...
by MicroBob
Wed Dec 09, 2020 6:22 am
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 746

Re: Question about setting up Zeiss DIC

Hi Pete,
I have never compared that methodically. Since changeing back and forth is time consuming one would have to take photos with switched of automatics to really compare the results.

Bob
by MicroBob
Tue Dec 08, 2020 9:11 pm
Forum: Microscopes and optics
Topic: Question about setting up Zeiss DIC
Replies: 7
Views: 746

Re: Question about setting up Zeiss DIC

Hi, Zeiss said to use oil immersion on the condenser front lens at all magnifications for your DIC system. Water immersion comes close to oil immersion and it works dry too, but of cause with drawbacks. When using immersion make sure that no fluid enters the fine thread that attatches the top lens. ...
by MicroBob
Tue Dec 08, 2020 8:33 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 810

Re: Experiments with botanic stains

In the german forum the Wacker W3A variations are very popular, actually have been varied, developed and improved by a couple of members. These are very very good stains but as well as Etzold FCA they are quite colourful, over time may be a bit overly colourful. When Rolf-Dieter-Müller, one of the s...
by MicroBob
Tue Dec 08, 2020 5:18 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 810

Re: Experiments with botanic stains

I have showed my hand /cylinder microtome and the blade holder I have developed here. I actually have a hand microtome for sale.

viewtopic.php?f=16&t=7065&p=62601&hilit ... der#p62601
by MicroBob
Tue Dec 08, 2020 4:29 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 810

Re: Experiments with botanic stains

Hi Wes,
this was cut embedded in a slit carrot with a cylinder microtome with my 3D-printed blade holder. The leaf sectioned very well.
The SFC-mix geves the yellow, the ink the black and blue tones.

Bob
by MicroBob
Tue Dec 08, 2020 3:59 pm
Forum: Specimens, samples and slides
Topic: Experiments with botanic stains
Replies: 10
Views: 810

Experiments with botanic stains

Hi together, in former times there was a stain called "Kernschwarz" of unknown formulation. It stained unlignified cell walls very specifically. Today it is not available any more but it is thought to be based on gallic acid and an iron salt, like iron gall ink. This made me think how iron gall ink ...
by MicroBob
Sun Dec 06, 2020 7:11 pm
Forum: Illumination Techniques
Topic: Oblique filter suggestions
Replies: 12
Views: 1229

Re: Oblique filter suggestions

Yes, I placed it in the filter holder unter the condenser. When I place one of the aluminium discs on the acrylic glass disc I get a hollow cone of light from the gap between outside aluminium disc and inside filter holder. These filter holders are placed not alike. The position doesn't matter much ...
by MicroBob
Sun Dec 06, 2020 11:32 am
Forum: Illumination Techniques
Topic: Oblique filter suggestions
Replies: 12
Views: 1229

Re: Oblique filter suggestions

Hi Glen, I dug this out for you. The aluminium discs are from 0,4mm foil, punched out with an expensive french double punch set on a piece of wood end grain. Oblique and COL are probably let down by their improvised use. If one would dedicate a turret condenser with stops for specific objectives jus...