Search found 1975 matches

by MicroBob
Wed Apr 15, 2020 5:51 am
Forum: Pictures and Videos
Topic: A few botanic cross sections
Replies: 18
Views: 574

Re: A few botanic cross sections

It appears as if your stains have mixed and formed a new colour instead of attatching to different structures. Have you tried the stains alone and have you tried to differntiate them by removing part of the staining so lightly stained structures are destained?

Bob
by MicroBob
Wed Apr 15, 2020 5:43 am
Forum: Microscopes and optics
Topic: Lamp for old light microscope
Replies: 7
Views: 333

Re: Lamp for old light microscope

Hi Mike, these tea lights give a light source of quite small diameter. The condensers are typically made to be illuminated with a parallel bundel of light rays of ca. 30mm diameter. With a single LED and no diffusor you will probably see an image with a lot of contrast and little resolution because ...
by MicroBob
Tue Apr 14, 2020 5:23 pm
Forum: Pictures and Videos
Topic: A few botanic cross sections
Replies: 18
Views: 574

Re: A few botanic cross sections

Hi Daruosh,
nice even sections! The first one could have been a Sansevieria, right?

Bob
by MicroBob
Mon Apr 13, 2020 7:03 pm
Forum: Introduce yourself
Topic: Introduction
Replies: 7
Views: 496

Re: Introduction

Hi Kirby, welcome to the forum! Perhaps you find time to show a couple of your collertors microscopes and their accessories. It would also be interesting to see some images taken with them. Your idea not to use the mercury lamp is for sure a good one. They were meant to be switched on for a whole da...
by MicroBob
Mon Apr 13, 2020 4:41 pm
Forum: Identification help
Topic: Odd structure, any ideas?
Replies: 5
Views: 372

Re: Odd structure, any ideas?

Chris Dee wrote:
Mon Apr 13, 2020 3:02 pm
Didn't expected there to be more than one possible candidate with this shape :shock:
That's microscopy! :lol:
by MicroBob
Mon Apr 13, 2020 2:13 pm
Forum: Identification help
Topic: Odd structure, any ideas?
Replies: 5
Views: 372

Re: Odd structure, any ideas?

by MicroBob
Mon Apr 13, 2020 7:02 am
Forum: Introduce yourself
Topic: Adding / requiring member locations?
Replies: 15
Views: 917

Re: Adding / requiring member locations?

For argument's sake, let's say I am in the outskirts of Imperial, Texas, USA. Be that true or false, how does that change anything for this discussion? If it doesn't, why require it? The microscope market, new and used, sources for supplies and widely used techniques differ a lot from place to plac...
by MicroBob
Mon Apr 13, 2020 6:02 am
Forum: Camera systems and imaging
Topic: Top Tier vs Mid Range DSLR Cameras
Replies: 7
Views: 447

Re: Top Tier vs Mid Range DSLR Cameras

The microscope image's information content vearies with the magnification. Fpr a 100:1 oil immersion objective only a couple of MP are needed to capture all that is in the image. The sensor format of the camera is an equivalent of the light capturing ability. As long as there is plenty light there i...
by MicroBob
Sat Apr 11, 2020 7:25 pm
Forum: My microscope
Topic: Microscopes in use at the Star Party
Replies: 5
Views: 494

Re: Microscopes in use at the Star Party

Collecting micro meteorites is not that easy. One finds a lot of magnetic sperical objects but closer examination and comparison to Jon Larsens photos shows that they are terrestial. I do quite a bit of heavy angle grinding and welding and occasionally fire up my charcoal forge for a bit of blackmit...
by MicroBob
Sat Apr 11, 2020 6:12 pm
Forum: My microscope
Topic: Microscopes in use at the Star Party
Replies: 5
Views: 494

Re: Microscopes in use at the Star Party

Hi dunk, that is a nice presentation for indoors astronomers! A related topic are the micro meteorites that were made popular by Jon Larsen and his book "In Search of Stardust". The Leitz dissection microscope is really nice too. The first instruments Carl Zeiss made from 1946 on were of this genera...
by MicroBob
Sat Apr 11, 2020 5:18 am
Forum: Specimens, samples and slides
Topic: River diatoms and "bubbles"
Replies: 19
Views: 724

Re: River diatoms and "bubbles"

Hi Doron,
nice catch!
Could these cylinders be the girdle bands of centrales diatoms? So valves and circular objects match in numbers so this could be true?

Bob
by MicroBob
Sat Apr 11, 2020 5:11 am
Forum: Pictures and Videos
Topic: Fluorescent Microorganisms
Replies: 3
Views: 253

Re: Fluorescent Microorganisms

Hi Rob,
great images! This is something I have on my to-do list too.

Bob
by MicroBob
Sat Apr 11, 2020 5:10 am
Forum: My microscope
Topic: 1923 Leitz Travelling Microscope
Replies: 10
Views: 546

Re: 1923 Leitz Travelling Microscope

ancient low mag. simple dissecting microscopes which are useful for examining e.g. meteorite specimens at public star parties Interesting use of these instruments! I have been wondering for a while whether they still had a use today. Often new designs replace older ones and it is no longer in view ...
by MicroBob
Fri Apr 10, 2020 5:51 pm
Forum: My microscope
Topic: 1923 Leitz Travelling Microscope
Replies: 10
Views: 546

Re: 1923 Leitz Travelling Microscope

Again a very beautiful microscope in great condition! I like the fine focu knob with scale in plated metal and the roundeg knurls everywhere. These are really nice to grip, smooth and still with a lot of puchase. This type of knurling is long gone, the bombed knurling wheels difficult to obtain toda...
by MicroBob
Fri Apr 10, 2020 1:16 pm
Forum: My microscope
Topic: 1923 Leitz Travelling Microscope
Replies: 10
Views: 546

Re: 1923 Leitz Travelling Microscope

These brass parts were painted with a stained shellac. This doesn't hold up too well under the influence of sweat and abrasion an the microscopes start to look really used after a while. When they are put out of use the plain polished brass starts to discolour. And when such an istrument gets in the...
by MicroBob
Fri Apr 10, 2020 7:21 am
Forum: My microscope
Topic: Zeiss mystery scope
Replies: 8
Views: 480

Re: Zeiss mystery scope

I don't know precisely the naming of Zeiss in the early post war years as there was legal dispute between Zeiss Jena and Zeiss Obenkochen+Göttingen but 1945-1990 will be about right. The measuring microscopes usually have a single objective in a ridgid block, not a revolving nosepiece. Is it possibl...
by MicroBob
Fri Apr 10, 2020 6:49 am
Forum: My microscope
Topic: 1923 Leitz Travelling Microscope
Replies: 10
Views: 546

Re: 1923 Leitz Travelling Microscope

Hi dunk, this is a beautiful instrument and case! It probably prooved to tiresome or difficult to actuall use it in the field and at the same time nobody had the bad idea to put it in a damp basement for storage. This design was quite common in the early 20th century, Reichert and Zeiss had similar ...
by MicroBob
Thu Apr 09, 2020 7:50 pm
Forum: My microscope
Topic: Zeiss mystery scope
Replies: 8
Views: 480

Re: Zeiss mystery scope

Hi Bill,
the long dovetailed guides look like those of a measuring microscope for sure. I would first try to decide whether it is Zeiss West or Zeiss Jena to narrow down the choices.
The stand would be nice for stacking of bigger objects.

Bob
by MicroBob
Thu Apr 09, 2020 5:20 pm
Forum: Pictures and Videos
Topic: human hair bulb - Oblique polarized + COL illumination
Replies: 4
Views: 268

Re: human hair bulb - Oblique polarized + COL illumination

Hi Daroush,
interesting subject and good contrast technique! The COL looks like DIC here. COL is a bit fiddly to setup in my experience. Perhaps you could reduce the vignetting by image editing.

Bob
by MicroBob
Thu Apr 09, 2020 5:16 pm
Forum: Camera systems and imaging
Topic: Adaptors from 50 to 500$..how is it possible ?
Replies: 29
Views: 3404

Re: Adaptors from 50 to 500$..how is it possible ?

Hi Marco,
this looks China made and might be available for a lot less somewhere else.

Bob
by MicroBob
Thu Apr 09, 2020 12:30 pm
Forum: Sandbox
Topic: 3D printed microscope holder, and some examples
Replies: 8
Views: 613

Re: 3D printed microscope holder, and some examples

Now I can see the pictures in the first post!

Bob
by MicroBob
Wed Apr 08, 2020 2:52 pm
Forum: Sandbox
Topic: 3D printed microscope holder, and some examples
Replies: 8
Views: 613

Re: 3D printed microscope holder, and some examples

Hi Antonio, I can't see the pictures. This forum has it's own image host for up to 500kb pictures with max 1024 pixels image size. This adapter is probably th best design available on thingiverse. There is a version 1.1 too. As typical for Thingiverse most designs are clearly not made by someone who...
by MicroBob
Wed Apr 08, 2020 7:36 am
Forum: Specimens, samples and slides
Topic: reflected light silicon chip die
Replies: 39
Views: 8925

Re: reflected light silicon chip die

Hi Michael, I haven't experimented a lot with these setups so I don't have much experience. What I can tell is: - that contrast is always a bit problematic due to scatteded light - the ring mirror is different for different objectives - the ring mirror has to sit on the right height - the light qual...
by MicroBob
Wed Apr 08, 2020 2:32 am
Forum: Illumination Techniques
Topic: I Use a Top Stage Light
Replies: 4
Views: 286

Re: I Use a Top Stage Light

Hi Don, for sure this is dark field as long as you put your object on a dark non reflective surface. You can find a photo of radiolaria raw material in this forum that I took with an even simpler setup. The Zeiss epi dark field kits even contained stops that allowed to adjust the illumination to les...
by MicroBob
Tue Apr 07, 2020 6:35 pm
Forum: Microscopes and optics
Topic: Zeiss microscope
Replies: 15
Views: 416

Re: Zeiss microscope

Often it is more economical to wait for an offer that already includes most of the wanted bits. The same microscope with phase contrast components wouldn't be much more expensive. A model with trino tube wouldn't cost as much more as a trino would add when bought separately.
by MicroBob
Tue Apr 07, 2020 5:23 pm
Forum: Microscopes and optics
Topic: Zeiss microscope
Replies: 15
Views: 416

Re: Zeiss microscope

Hi, this is a Zeiss Standard, probably model 14. The lamp might be missing. There is an ergonomical tube mounted - if you don't need it you can sell it. Phase contrast components and many other accessories are available without problem. Trino heads are available but not cheap. The Zeiss Standard has...
by MicroBob
Tue Apr 07, 2020 4:24 pm
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 416

Re: Pine needle-fluorescence,

Many mountants have quite a bit of autofluorescence which makes it a bit difficult to get contrasty slides for fluorescence application. Is PEG1500 usable a a mountant or just for embedding for sectioning? I compared a couple of mountants for blue excitation fluorescence here: http://www.microbehunt...
by MicroBob
Tue Apr 07, 2020 11:16 am
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 416

Re: Pine needle-fluorescence,

Hi Seb,
great images! Are these permanent mounted or fresh?

Bob
by MicroBob
Tue Apr 07, 2020 6:04 am
Forum: My microscope
Topic: IOR MC9 research microscope
Replies: 11
Views: 792

Re: IOR MC9 research microscope

Congratulations to this great microscope! It probably is at least as rare as the Zeiss Axiomat. Compared to a Zeiss Universal or Leitz Orthoplan it is a microscope with similar user value but so much more value as a collectors piece. I find it quite incredible to see what a complicated and seemingly...
by MicroBob
Mon Apr 06, 2020 7:30 pm
Forum: Miscellaneous
Topic: Repository of exotic diatoms?
Replies: 15
Views: 517

Re: Repository of exotic diatoms?

If I were even lazier I would just put the rotor of my hand centrifuge in a bench drill and use it that way. My main machine in the workshop is powered over a frequency converter and can be regulated from zero up. My first centrifuge was home built and I used it in a strong steel construction drill ...