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by MichaelG.
Sun Mar 11, 2018 7:09 pm
Forum: Beginner's corner
Topic: Another Zeiss Tessovar Question
Replies: 6
Views: 1375

Re: Another Zeiss Tessovar Question

There are four working distances [according to the supplementary in use] I. 320mm II. 150mm III. 75 mm IV. 36mm Therefore, if we set focus for (I) on the table (II) needs a lift of 170mm [320-150] (III) needs a lift of 245mm [320-75] (IV) needs a lift of 284mm [320-36] Q.E.D. MichaelG. http://www.sa...
by MichaelG.
Sun Mar 11, 2018 9:41 am
Forum: Identification help
Topic: The very weirdest thing I've yet seen
Replies: 30
Views: 3834

Re: The very weirdest thing I've yet seen

Thanks, Lilly

MichaelG.
by MichaelG.
Sun Mar 11, 2018 8:20 am
Forum: Identification help
Topic: The very weirdest thing I've yet seen
Replies: 30
Views: 3834

Re: The very weirdest thing I've yet seen

Lilly,

Please forgive my ignorance of your local geography:

Is this the 'RSW lake' from which you are collecting such wonders ?
https://www.google.com/maps/@26.5391769 ... n-GB&gl=US
http://www.wikiwand.com/en/Southwest_Fl ... al_Airport

MichaelG.
by MichaelG.
Sat Mar 10, 2018 8:17 pm
Forum: Identification help
Topic: The very weirdest thing I've yet seen
Replies: 30
Views: 3834

Re: The very weirdest thing I've yet seen

What a fascinating creature !!

The still images give the surface structure, which is sadly missing from the video:
... But the video shows that astonishing, and beautiful, motion.

I was captivated.

Sadly, I don't have the faintest idea what it might be.

MichaelG.
by MichaelG.
Thu Mar 08, 2018 11:06 am
Forum: Digital processing
Topic: Stacking Software
Replies: 41
Views: 20756

Re: Stacking Software

For easy reference: I have copied the text from Zerene's FAQ, concerning RAW files MichaelG. . . Does Zerene Stacker handle raw files? To process raw files takes two separate steps. First you convert the raw files to some RGB format, typically TIFF, and then you stack the TIFF files. For highest qua...
by MichaelG.
Tue Mar 06, 2018 2:27 pm
Forum: Digital processing
Topic: Stacking Software
Replies: 41
Views: 20756

Re: Stacking Software

Pat Thielen wrote:edit: Does PICOLAY work with RAW files?
The answer would appear to be "some of them"
http://www.picolay.icbm.de/picolay-manual.pdf

My Panasonic RW2 files are not listed.
... but that's largely irrelevant to me, as it's 'Windows only' software.

MichaelG.
by MichaelG.
Tue Mar 06, 2018 8:32 am
Forum: Digital processing
Topic: Stacking Software
Replies: 41
Views: 20756

Re: Stacking Software

Hello Pat I would recommend that you try Zerene Stacker http://zerenesystems.com/cms/stacker It's versatile, and seems very stable on my Macs. MichaelG. . . As an example; here's a tricky little subject that I tried last week: Moss Mite : Cepheus dentratus : collected by David Copestake, and current...
by MichaelG.
Mon Mar 05, 2018 11:00 pm
Forum: Resources (online, books etc.)
Topic: Color Balance in Digital Imaging
Replies: 2
Views: 1594

Re: Color Balance in Digital Imaging

Excellent article, thanks for the link.

MichaelG.
.

I note that LED illumination is not mentioned.
... We can probably read something into that.
by MichaelG.
Mon Mar 05, 2018 6:54 pm
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

Lilly Begonia wrote:
MichaelG. wrote: Yes it would Michael, the camera slips around in the eyepiece tube, even after I zip-tied it's cable down. I wish there was a set screw in that tube.
Here's one of life's little miracles >>> https://en.m.wikipedia.org/wiki/Blu_Tack

MichaelG.
by MichaelG.
Mon Mar 05, 2018 6:46 pm
Forum: Miscellaneous
Topic: Well, another issue with my microscope
Replies: 6
Views: 1948

Re: Well, another issue with my microscope

The condenser should indeed stay where you put it. There should be a screw you can tighten somewhere. One should not adjust the light intensity by raising and lowering the condenser. Adjust the light intensity by turning the dimmer. Condenser should be placed a couple of mm below the slide and left...
by MichaelG.
Mon Mar 05, 2018 5:50 pm
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

Hello again, Lilly

This is very rough, but should give you the general idea:
100 divisions of 0.01mm
100 divisions of 0.01mm
IMG_1847.JPG (403.1 KiB) Viewed 1154 times
... obviously it would be better to align the scale with the camera's horizontal.

MichaelG
by MichaelG.
Mon Mar 05, 2018 1:49 pm
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

Thank you so very much Michael! Well I got out that calibration slide, put it on the stage, turned to my lowest power objective and focused on it. Here's what I see in the middle of the circle: https://s26.postimg.org/w3yplqvax/calibrating_slide.jpg There are no numbers there. I'm not sure how to u...
by MichaelG.
Mon Mar 05, 2018 7:09 am
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

Lilly, I must first emphasise [ shout from the roof-tops ] that I think you are doing a fantastic job, despite the obvious frustration with your chosen equipment. To understand the calibration, it's probably worth starting with the camera sensor: This is specified as having 2560x1922 pixels, on a pi...
by MichaelG.
Sun Mar 04, 2018 4:20 pm
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

Couldn't ask for much better than that, Lilly MichaelG. . One point that I must mention: Unless I am very much mistaken, the two objectives that you mention are actually 10x and 40x Omax specifies a range of magnifications, but these numbers are the product of 'objective x eyepiece' http://www.micro...
by MichaelG.
Sun Mar 04, 2018 2:00 pm
Forum: Pictures and Videos
Topic: First water bear found elsewhere
Replies: 15
Views: 1277

Re: First water bear found elsewhere

I enlarged these shots as Michael suggested but I think it blurs them a bit and the sharp focus is somewhat lost. Could I just check, Lilly ? ... How are you enlarging the images ? When I suggested getting the bear bigger in the frame, I was thinking of higher magnification in the microscope; and y...
by MichaelG.
Sat Mar 03, 2018 9:50 pm
Forum: Illumination Techniques
Topic: Dark field illumination: Questions and answers
Replies: 65
Views: 13945

Re: Dark field illumination: Questions and answers

My questions: 3) Is this df condenser dependant on a specific tube-length microscope? Or are substage condensers independent of a stands: tube length spec? ... charlie g I can see no optical reason why the condenser should be specific to a certain tube length. ... I'm always ready to learn, though ...
by MichaelG.
Sat Mar 03, 2018 9:37 pm
Forum: Pictures and Videos
Topic: second water bear today, a good one
Replies: 3
Views: 558

Re: second water bear today, a good one

Nice one, Lilly

If you can get the bear a little bigger in the frame, they should be even better.
... It seems a pity to waste the camera resolution.

MichaelG.
by MichaelG.
Sat Mar 03, 2018 9:38 am
Forum: Illumination Techniques
Topic: Dark field illumination: Questions and answers
Replies: 65
Views: 13945

Re: Dark field illumination: Questions and answers

Cooke always had an optical base and still does. They are one of the best in the world. I'm not sure where or when the Cooke camera lens and Cooke microscope lens concerns cleaved off but the ability of a company with Cooke in the name to make optics as good as anyone, isn't surprising. Useful 'tim...
by MichaelG.
Sat Mar 03, 2018 9:06 am
Forum: Beginner's corner
Topic: Mounting medium question
Replies: 17
Views: 2753

Re: Mounting medium question

I've been searching for evidence to support my own observations of crystallisation in Numount, and have found this compilation: Some works by Douglas Turnbull Richardson http://www.microscopy-uk.eu/diatomist/dtr.pdf The two paragraphs at the end of page 17 are well worth noting [as is anything writt...
by MichaelG.
Fri Mar 02, 2018 6:04 pm
Forum: Beginner's corner
Topic: Mounting medium question
Replies: 17
Views: 2753

Re: Mounting medium question

Mike and John Many thanks for the replies Just to clarify: I have never personally used Numount ... my experiences are exclusively as an observer of others' work. I have a sufficient supply of Canada Balsam to keep me amused for years; but have sometimes wondered about trying Numount. MichaelG. . P....
by MichaelG.
Fri Mar 02, 2018 2:50 pm
Forum: Beginner's corner
Topic: Mounting medium question
Replies: 17
Views: 2753

Re: Mounting medium question

... and 'Numount' resinous medium (which is my first-choice for permanent-mounts of sectioned & stained plant tissue slides)... I am no expert in these matters, John ... but I would be wary of using Numount for anything you want to archive for posterity. As a member of the Postal Microscopical Soci...
by MichaelG.
Thu Mar 01, 2018 8:23 am
Forum: Introduce yourself
Topic: Hello from the frozen Northwest!
Replies: 15
Views: 4765

Re: Hello from the frozen Northwest!

Welcome-- You get a lot of brownie points for earning your degree later in life.. from me at least!!! +1 MichaelG. [ In surprisingly cold and snowy England ] . Edit: Perhaps Lichenologists have the same pioneering spirit as their subjects: http://moticeuropeamericasblog.blogspot.co.uk/2018/02/trans...
by MichaelG.
Wed Feb 28, 2018 6:06 pm
Forum: Illumination Techniques
Topic: 43x very dim
Replies: 38
Views: 5540

Re: 43x very dim

Hobbyst46 wrote:Is German eBay a separate site from "English/American" eBay?
Yes ... but you can log-in with the same username & password.
https://www.ebay.de

MichaelG.
by MichaelG.
Tue Feb 27, 2018 11:56 pm
Forum: Illumination Techniques
Topic: 43x very dim
Replies: 38
Views: 5540

Re: 43x very dim

... And i followed a youtube video on Kohler illumination, but I either notice no difference, or I am doing it incorrectly. If you have an iOS device ... This is a very helpful interactive tutor Koehler Tutor by Andrew Barlow https://itunes.apple.com/gb/app/koehler-tutor/id1281328178?mt=8 Highly re...
by MichaelG.
Tue Feb 27, 2018 11:42 pm
Forum: Beginner's corner
Topic: Word to the Wise: Lessons Learned
Replies: 18
Views: 4154

Re: Word to the Wise: Lessons Learned

Thanks for posting the links, Charles
... but unfortunately they are both the same.

MichaelG.
.
Edit: Presumably the 'Different Way' link is:
viewtopic.php?t=4227
by MichaelG.
Mon Feb 26, 2018 7:36 pm
Forum: Miscellaneous
Topic: Sometimes my focus knob doesn't seem to work.
Replies: 10
Views: 2155

Re: Sometimes my focus knob doesn't seem to work.

Well it's official, it's not the software, it's the scope itself. I was looking at a rotifer just now, and the focus issue happened again, so I looked through the eyepiece, and it was happening there too. I wrote Elizabeth at MicroscopeNet.com and told her of the problem asking what might be done a...
by MichaelG.
Mon Feb 26, 2018 7:40 am
Forum: Microscopes and optics
Topic: Just bought a 402a phase condenser
Replies: 10
Views: 1548

Re: Just bought a 402a phase condenser

Thanks for the link, John
That's a very useful reference document.

MichaelG.
by MichaelG.
Sun Feb 25, 2018 11:20 pm
Forum: Miscellaneous
Topic: Sometimes my focus knob doesn't seem to work.
Replies: 10
Views: 2155

Re: Sometimes my focus knob doesn't seem to work.

... in the past there has been quite serious lag while panning slides with ToupLite. My guess is that both problems are mainly attributable to the USB_3 vs USB_2 speed issue that we have already discussed. ToupLite may be making things worse though ... It does seem very much 'the poor relation' A t...
by MichaelG.
Sun Feb 25, 2018 7:07 pm
Forum: Miscellaneous
Topic: Sometimes my focus knob doesn't seem to work.
Replies: 10
Views: 2155

Re: Sometimes my focus knob doesn't seem to work.

The obvious check: What do you see, visually, through the eyepiece ? If it's the same effect, then the problem is with the microscope. If not ... then it's probably the software. How does the screen image behave if you pan the stage ? ... Is it 'real time' or does it lag ? MichaelG. . Edit: I've jus...
by MichaelG.
Sat Feb 24, 2018 3:10 pm
Forum: Pictures and Videos
Topic: Found this cute little cilliate this morning
Replies: 5
Views: 613

Re: Found this cute little cilliate this morning

... Oh, and I got ToupLite to stop locking up on me! Yaaaaayyy! I took the camera off that 13 port USB hub and plugged into a port on the Mac, no more problems with locking up, but the video still sucks. That seems logical: (a) General wisdom is that cameras perform better when connected direct (b)...