Search found 1897 matches

by MichaelG.
Wed May 13, 2020 7:37 pm
Forum: My microscope
Topic: New Amscope SMT2
Replies: 6
Views: 147

Re: New Amscope SMT2

Thanks geo man. I use stacking in my astrophotography images ' We take like 50 3min images of galaxies 'nebulas ect and stack them to reduce noise and build up the image. Have to look into that for this hobby. Thanks Bob Different concept I'm afraid ... in microscopy it's focus [ Z axis ] stacking ...
by MichaelG.
Tue May 12, 2020 9:25 pm
Forum: Beginner's corner
Topic: Hi I'm new and was thinking off buying a microscope
Replies: 7
Views: 219

Re: Hi I'm new and was thinking off buying a microscope

Greetings, Joe I was intrigued by your question, so thought I would look around to see what’s involved. This site is surprisingly informative: http://www.sandhillvet.co.uk/html/pigeon_service_management_diseases.html ... It looks like the basic testing is a matter of identification and counting. ......
by MichaelG.
Tue May 12, 2020 11:21 am
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

Okay, thanks. Unfortunately I can't afford several unsuccessful experiments, so I if there's anyone else who has further ideas, I'd really appreciate it! Your experiments could start with simply lifting the eyepiece a few millimetres and checking for any change in performance. ... a piece of tape o...
by MichaelG.
Tue May 12, 2020 11:18 am
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

Okay, thanks. Unfortunately I can't afford several unsuccessful experiments, so I if there's anyone else who has further ideas, I'd really appreciate it! Your experiments could start with simply lifting the eyepiece a few millimetres and checking for any change in performance. ... a piece of tape o...
by MichaelG.
Tue May 12, 2020 5:15 am
Forum: Introduce yourself
Topic: Hello and greetings from Kuala Lumpur, Malaysia
Replies: 4
Views: 153

Re: Hello and greetings from Kuala Lumpur, Malaysia

Welcome to the forum

I, for one, look forward to learning from your experience with fluorescence.
... an area very little known to me, but rather intriguing.

MichaelG.
by MichaelG.
Mon May 11, 2020 2:41 pm
Forum: Microscopes and optics
Topic: De-doming (shaving) LED's
Replies: 7
Views: 193

Re: De-doming (shaving) LED's

Neatly done, Chris
... and definitely looks worth the effort.

[not too sure about the ‘risk to life’ but you obviously survived intact]

MichaelG.
by MichaelG.
Sun May 10, 2020 9:55 pm
Forum: Camera systems and imaging
Topic: Raspberry Pi High Quality Camera
Replies: 14
Views: 814

Re: Raspberry Pi High Quality Camera

You are right, for first class imaging you have to use a correction eyepiece. A Pentax Q with identical chip size and an 8,5mm objective works fine. Sorry ... I’m late back to the discussion, and it looks like everything is sorted now : corrective eyepiece and afocal imaging using a suitable focal ...
by MichaelG.
Sun May 10, 2020 5:30 pm
Forum: Camera systems and imaging
Topic: PWM and banding
Replies: 3
Views: 172

Re: PWM and banding

Mmm ... only if it significantly changed the exposure time on your camera, or meant that the LED was on for longer.

The banding is essentially a ‘beat frequency’ problem, but changing the mark/space ratio of the PWM can mask it.

MichaelG.
by MichaelG.
Sun May 10, 2020 6:39 am
Forum: Pictures and Videos
Topic: Dandelion pappus ("parachute") details
Replies: 5
Views: 348

Re: Dandelion pappus ("parachute") details

Thank you, Charles

I am awestruck, by both the dandelion and your imaging !

MichaelG.
by MichaelG.
Sun May 10, 2020 6:15 am
Forum: Beginner's corner
Topic: What are the practical differences between trinocular light split settings when imaging? (E.g. 100:0 / 20:80)
Replies: 8
Views: 394

Re: What are the practical differences between trinocular light split settings when imaging? (E.g. 100:0 / 20:80)

I am sure your interpretation is correct

... but I doubt there is a useful theoretical analysis available to predict the practical difference.

Quality and cleanliness of the components will over-ride any theory !!

MichaelG.
by MichaelG.
Sun May 10, 2020 5:44 am
Forum: Pictures and Videos
Topic: Arachnoidiscus
Replies: 16
Views: 697

Re: Arachnoidiscus

Here is a link that Arachnoidiscus fans might find interesting: https://www.researchgate.net/publication/264391961_Optical_Properties_of_Diatom_Nanostructured_Biosilica_in_Arachnoidiscus_sp_Micro-Optics_from_Mother_Nature Thanks for the link; I have just downloaded it ... That’s today’s reading & p...
by MichaelG.
Sun May 10, 2020 4:59 am
Forum: Camera systems and imaging
Topic: Raspberry Pi High Quality Camera
Replies: 14
Views: 814

Re: Raspberry Pi High Quality Camera

The sensor 1/2" Please forgive the pedantry, but, according to Sony it is a 1/2.3 sensor ... which is significantly smaller than 1/2 [but still a very useable size] https://www.sony-semicon.co.jp/products/common/pdf/IMX477-AACK_Flyer.pdf . Diagonal 7.857 mm (Type 1/2.3) 12.3 Mega-Pixel CMOS Image S...
by MichaelG.
Sat May 09, 2020 10:40 pm
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

The Leica BME microscope uses the E series objectives ... so it may be worth noting the part numbers for the E2 and E3 versions for future reference:

http://www.mcbainsystems.com/pdf/Leica_BME_broch.pdf

MichaelG.
by MichaelG.
Sat May 09, 2020 9:02 pm
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

... but how do I know exactly what to look for? I’m truly sorry, but I don’t have the skill-set to teach you that The Leitz memo gives some very strong clues that the mechanical tube length issue can be ‘worked-around’ by selecting the right components, but you will almost certainly need to experim...
by MichaelG.
Sat May 09, 2020 6:03 pm
Forum: Pictures and Videos
Topic: Arachnoidiscus
Replies: 16
Views: 697

Re: Arachnoidiscus

:o I see what you mean

Thanks for the additional link

MichaelG.
by MichaelG.
Sat May 09, 2020 5:50 pm
Forum: Pictures and Videos
Topic: Arachnoidiscus
Replies: 16
Views: 697

Re: Arachnoidiscus

Thanks sinabro, they are quite amazing diatoms Just rediscovered a 2017 post by hkv that seriously raises the bar! I will of course be making several more attempts to capture its beauty - however, I can't see how I will get near to what hkv has done. Still, half the fun is seeing how far one can ge...
by MichaelG.
Sat May 09, 2020 4:56 pm
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

And larger versions. The first one was taken with the 4x objective for 170mm system. https://imgur.com/W96uwB0 https://imgur.com/QM3aEHh https://imgur.com/pCHs39b 4x looks free of Lateral Chromatic Aberration, but is not very sharp at all 10x has a lot of CA at the sides, but is good at the centre ...
by MichaelG.
Sat May 09, 2020 4:39 pm
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

What should I be looking for in the memo? Even though I've owned a microscope for years I didn't use it a lot and I'm definitely new to more technical aspects. ... You should be looking to get a basic understanding of the relevance [or otherwise] of the mechanical tube length : 170mm vs 160mm The f...
by MichaelG.
Sat May 09, 2020 1:19 pm
Forum: Camera systems and imaging
Topic: Help with Leitz Laborlux-12 + DSLR setup
Replies: 11
Views: 412

Re: Help with Leitz Laborlux-12 + DSLR setup

Welcome aboard !! This famous inter-office memorandum should probably be your first reference: http://www.science-info.net/docs/leitz/Leitz-160mm-Memo.pdf After that, try taking some boring shots of something like a stage micrometer. ... I do like the arty one, but it’s difficult to analyse anything...
by MichaelG.
Fri May 08, 2020 10:13 pm
Forum: Pictures and Videos
Topic: Aspects of a Bite of Apple
Replies: 2
Views: 253

Re: Aspects of a Bite of Apple

Very interesting texture, Don

The first image works best for me.

MichaelG.
by MichaelG.
Thu May 07, 2020 10:30 pm
Forum: Miscellaneous
Topic: PVC and PET microplastics in caddisfly
Replies: 6
Views: 601

PVC and PET microplastics in caddisfly

Interesting report on lab work here:

PVC and PET microplastics in caddisfly ( Lepidostoma basale ) cases reduce case stability | SpringerLink

https://link.springer.com/article/10.10 ... 20-08790-5

MichaelG.
by MichaelG.
Thu May 07, 2020 8:09 pm
Forum: Microscopy accessories
Topic: Power supply woes
Replies: 17
Views: 536

Re: Power supply woes

75RR wrote:
Thu May 07, 2020 5:51 pm
I remember they used to be around $8, which I thought excessive even then!
I was amazed to see that bulb has an ordinary bayonet mount, not a tricky prefocus flange.

MichaelG.
by MichaelG.
Thu May 07, 2020 12:35 pm
Forum: Microscopy accessories
Topic: Power supply woes
Replies: 17
Views: 536

Re: Power supply woes

Does that PSU have a Variac [or similar] under the knob ?
... If so, the wiper brush may be worn, or the track may dirty.

MichaelG.

https://uk.rs-online.com/web/c/power-su ... s/variacs/
by MichaelG.
Wed May 06, 2020 10:14 pm
Forum: Beginner's corner
Topic: objective nomenclature
Replies: 17
Views: 795

Re: objective nomenclature

Here’s a similar discussion, from 2009 : https://www.photomacrography.net/forum/viewtopic.php?t=8240&sid=bd711f22d513af3aa732e3d74c4171f1 If this statement is true: I´ve got the original Leitz booklet in front of me. In there are listed and explained all abbreviations the Leitz people have used duri...
by MichaelG.
Wed May 06, 2020 3:21 pm
Forum: Beginner's corner
Topic: objective nomenclature
Replies: 17
Views: 795

Re: objective nomenclature

So it looks like perhaps ‘normal’ means ‘ordinary/common-or-garden’ not anything geometrically special.

Confusion continues to reign !

MichaelG.
by MichaelG.
Wed May 06, 2020 1:08 pm
Forum: Beginner's corner
Topic: objective nomenclature
Replies: 17
Views: 795

Re: objective nomenclature

Looks like I will remain confused for a while longer I don't have a price list handy but I have seen one for the group of objectives listed for the AO/Reichert series 400 microscopes. They listed three sets of objectives over the 16 or so years that the microscopes remained on the market. 5 - Neopl...
by MichaelG.
Wed May 06, 2020 4:17 am
Forum: Camera systems and imaging
Topic: Using a ZWo ASI385MC with my American Optical 150
Replies: 2
Views: 289

Re: Using a ZWo ASI385MC with my American Optical 150

For convenience, here’s the specification [which looks very impressive]:
https://astronomy-imaging-camera.com/pr ... 85mc-color

It’s good to see these ‘astro’ cameras coming with T-mount threads
... that should make them very easily adaptable.

MichaelG.
by MichaelG.
Tue May 05, 2020 9:53 pm
Forum: Beginner's corner
Topic: Info on my objective please
Replies: 3
Views: 291

Re: Info on my objective please

Here’s a little more about G & S

http://rontocknell.blogspot.com/search?q=Conference

[no comic operas please]

MichaelG.
.

P.S. ___ 160/- means 160mm tube length [as you deduced] and “not fussy about coverslip thickness”
The pale blue ring is just an ergonomic identifier for that power.