Search found 1897 matches

by MichaelG.
Wed Apr 22, 2020 10:45 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 3694

Re: 40x SPlan woes

I believe this is it :
viewtopic.php?p=73981#p73981

MichaelG.
by MichaelG.
Wed Apr 22, 2020 9:54 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 3694

Re: 40x SPlan woes

Massively better result, Thomas !! Now we need to work-out why that could be. My guess is that the problem lies with your extension of the original photo-tube Consider this: The lensless MTV-3 is, I believe, too short and giving sub-optimal results The extended original photo-tube is giving worse re...
by MichaelG.
Wed Apr 22, 2020 9:28 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 3694

Re: 40x SPlan woes

An excellent comparative test, Thomas ... except for one failure in the ‘design‘ of the experiment Zeiss covers are 0.17 +/- 0.005 Generic covers are 0.13 to 0.17 But, what is the thickness of the actual generic cover you are testing ? ... In any one box they could be all thick, all medium, all thin...
by MichaelG.
Wed Apr 22, 2020 6:50 pm
Forum: Identification help
Topic: Need help unusual strand of eggs or what ever it is
Replies: 3
Views: 324

Re: Need help unusual strand of eggs or what ever it is

My guess is that they are Snail Eggs

...and that’s the total extent of my knowledge on the matter

Great image though !!

MichaelG.
by MichaelG.
Wed Apr 22, 2020 2:09 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3109

Re: Pollen Fine Detail

Here's an image or three of similarly prepared pollen from the 'Honesty' plant - Lunaria.annua That’s a fascinating comparison, John ... and demonstrates a significant point about our visual perception: The strong highlight on the normal image jumps forward, and seems quite intrusive ... Invert the...
by MichaelG.
Wed Apr 22, 2020 8:22 am
Forum: Microscopes and optics
Topic: A permanent grease for sealed microscope components: Krytox
Replies: 7
Views: 946

Re: A permanent grease for sealed microscope components: Krytox

Very impressive performance !!
Thanks for the link to the note.

MichaelG.
by MichaelG.
Wed Apr 22, 2020 8:14 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3109

Re: Pollen Fine Detail

I thought I'd show these images […] Also as an adjunct to a recent comment I made re the super-clarity/resolution of SEM images being an aid to the appreciation of just what we CAN see with 'ordinary' light microscopy rather than a source of envy! […] Hope you may find these interesting - I'm quite...
by MichaelG.
Wed Apr 22, 2020 7:53 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3109

Re: Pollen Fine Detail

Extraordinary work, John
... beautiful and very informative images

MichaelG.
by MichaelG.
Tue Apr 21, 2020 6:34 pm
Forum: Pictures and Videos
Topic: Stage control issues
Replies: 3
Views: 310

Re: Stage control issues

Thanks for clarifying that, Marco If it’s looseness and backlash, you may be able to adjust some of it out ... careful inspection of all the fixtures and fittings, and improve whatever you can ! and ... The application of some ‘damping grease’ might help. Sorry, I obviously misinterpreted the ‘judde...
by MichaelG.
Tue Apr 21, 2020 5:37 pm
Forum: Pictures and Videos
Topic: Stage control issues
Replies: 3
Views: 310

Re: Stage control issues

It looks like stick-slip [stiction] where something is not moving sweetly ... Effort is required to overcome the friction; but once moving, it carries-on.

It seems unlikely that there will be any useful adjustment, so a clean and re-grease may be all that you can do.

MichaelG.
by MichaelG.
Tue Apr 21, 2020 11:45 am
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1524

Re: WTB: Leitz K4 condenser lens

Rorschach wrote:
Tue Apr 21, 2020 9:15 am
Ok. I take full responsibility for the confusion :D
Very gracious of you
... It’s no problem: I just didn’t want to see you disappointed

MichaelG.
by MichaelG.
Tue Apr 21, 2020 8:44 am
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1524

Re: WTB: Leitz K4 condenser lens

Rorschach wrote:
Tue Apr 21, 2020 8:02 am
MichaelG do you still have the K4 and would you sell it?

I did send a question to Perl already at the other forum.

Edit: the images in your latest message are visible !
Crossed lines, I think

... I have never had a K4

My images are just a screenshot of what mrsonchus posted

MichaelG.
by MichaelG.
Tue Apr 21, 2020 7:42 am
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1524

Re: WTB: Leitz K4 condenser lens

Screenshot:
.
6ED6B82F-A92A-49C6-AFD6-4EF5074EF6F4.jpeg
6ED6B82F-A92A-49C6-AFD6-4EF5074EF6F4.jpeg (154.88 KiB) Viewed 1047 times
.

MichaelG.
by MichaelG.
Tue Apr 21, 2020 6:00 am
Forum: Microscopes and optics
Topic: Nikon Camera Lucida help required
Replies: 4
Views: 357

Re: Nikon Camera Lucida help required

That’s a really informative document

Thanks for the link 75RR

MichaelG.
by MichaelG.
Tue Apr 21, 2020 5:46 am
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1524

Re: WTB: Leitz K4 condenser lens

Rorschach wrote:
Tue Apr 21, 2020 4:36 am
The pic and info are not showing. :?:
They are still showing fine on my iPad

MichaelG.
by MichaelG.
Tue Apr 21, 2020 5:39 am
Forum: Camera systems and imaging
Topic: Advice on a dark field camera
Replies: 21
Views: 789

Re: Advice on a dark field camera

That’s a great start ... Keep ‘em coming

MichaelG.
by MichaelG.
Mon Apr 20, 2020 11:39 pm
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1524

Re: WTB: Leitz K4 condenser lens

mrsonchus wrote:
Mon Apr 20, 2020 11:29 pm

This is the one I had in my Orthoplan's condenser.....
What an interesting-looking lens
... ‘though maybe it’s just a trick of the light

I would love to see a sectional drawing, or the detailed formula.

MichaelG.
by MichaelG.
Mon Apr 20, 2020 10:31 pm
Forum: Camera systems and imaging
Topic: Olympus DP25
Replies: 6
Views: 371

Re: Olympus DP25

PCIe boards, to add FireWire 400 and/or 800 are readily available.

Note: The USB and FireWire protocols are very different ... so ‘adapters’ are best avoided.

MichaelG.

.
Sorry ... it must be bedtime ... I’ve only just noticed the word ‘laptop’
by MichaelG.
Mon Apr 20, 2020 9:13 pm
Forum: Camera systems and imaging
Topic: Help identifying this mount
Replies: 3
Views: 278

Re: Help identifying this mount

Just an observation : The use of two diametrically opposed screws is very silly

It makes one wonder about other aspects of the mechanical design and build-quality.

MichaelG.
by MichaelG.
Mon Apr 20, 2020 9:03 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 763

Re: Imaging with different coverglass thickness

Michael: they are probably expensive, I cannot see a price, but such chambers (from any brand) are the best and most convenient means of studying live cells in culture, on top of an inverted microscope. Typically just under 10 GBP each, in packs of 16 or 96 I have withdrawn my smilie of surprise , ...
by MichaelG.
Mon Apr 20, 2020 8:45 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 763

Re: Imaging with different coverglass thickness

To be honest, Andrew ... I had never heard of a #1 chambered coverslip :oops: So, I looked here for the spec. https://www.thermofisher.com/order/catalog/product/155361#/155361 ... and also saw the price I assume, and sincerely hope, that your colleagues know exactly what they are doing. MichaelG. . ...
by MichaelG.
Mon Apr 20, 2020 8:35 pm
Forum: Microscopy accessories
Topic: 25M female to RMS male
Replies: 6
Views: 460

Re: 25M female to RMS male

There are a couple of listings on ebay UK

Search for “rms male” to m25 adapter

MichaelG.

.
Sorry ... I’ve just read your comment more carefully. :oops:
by MichaelG.
Mon Apr 20, 2020 6:46 pm
Forum: Camera systems and imaging
Topic: Advice on a dark field camera
Replies: 21
Views: 789

Re: Advice on a dark field camera

Numanoid wrote:
Mon Apr 20, 2020 6:07 pm
Technically it comes native with an M42x0.75 mount so if you could source an adapter with that you could skip the others.
At the risk of stating the obvious ...
May I just remind folks that M42x0.75 is the standard T-mount thread

MichaelG.
by MichaelG.
Mon Apr 20, 2020 12:58 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 759

Re: More phase contrast photos of cleaned diatoms

Hobbyst46 wrote:
Mon Apr 20, 2020 12:46 pm
Willingly so. When I find a "standard", amphipleura pellucida (very rare here) or pleurosigma (supposedly common but missed it so far), and manage to mount them well.
:D :D :D
by MichaelG.
Mon Apr 20, 2020 11:33 am
Forum: My microscope
Topic: USB phone setup hacked together with 3D printer and Zeiss Base.
Replies: 3
Views: 410

Re: USB phone setup hacked together with 3D printer and Zeiss Base.

I've had this Plugable brand USb microscope for a while, but i have been less than satisfied with the factory ability to position the scope. […] Any way here is the abomination. Please do tell me what you think. I think it’s an excellent project, nicely executed You have identified the major failin...
by MichaelG.
Mon Apr 20, 2020 11:24 am
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 759

Re: More phase contrast photos of cleaned diatoms

Thanks for the reply, Doron I can think of three plausible reasons why Green might be considered optimum [as distinct from maximum] The human eye is most sensitive in the Green region many monochrome cameras are also tuned to that range most colour cameras use the Bayer mosaic, which has twice the n...
by MichaelG.
Sun Apr 19, 2020 10:35 pm
Forum: Microscopes and optics
Topic: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50
Replies: 20
Views: 803

Re: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50

https://www.microscopyu.com/tutorials/kohler Koehler makes it possible to produce even lighting of the specimen from a source which is not itself either large, diffuse, homogeneous, or a point. ... i.e. from, for example, a compact filament lamp. I count that as a clever trick MichaelG. . Edit : See...
by MichaelG.
Sun Apr 19, 2020 10:26 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 759

Re: More phase contrast photos of cleaned diatoms

Hobbyst46 wrote:
Sun Apr 19, 2020 8:51 pm
In principle, best resolution is expected under green light; here, the purpose was just aesthetics.
Hope it was achieved.
It certainly was achieved !

I’m a little puzzled by your ‘green light’ comment though ... would blue light not give better resolution?

MichaelG.
by MichaelG.
Sun Apr 19, 2020 8:12 pm
Forum: Microscopes and optics
Topic: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50
Replies: 20
Views: 803

Re: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50

So the Zeiss Standard I have seems to have the collector lens and frosted glass built into the female part of the light tube. So would I just have to adjust the distance between the LED and the frosted glass/ collector lens until I can get an image of the LED centered on the condenser front focal p...
by MichaelG.
Sun Apr 19, 2020 8:07 pm
Forum: Microscopes and optics
Topic: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50
Replies: 20
Views: 803

Re: High CRI LEDs - Olympus True Color, Nichia Optisolis and Yuji VTC D50

If you are starting from scratch, then you need to make an important design decision before you start: Is the illumination to be diffuse or a beam ? Could you give a page number in the book/pdf you gave on what you mean by the important design decision? I thought the design would be collimated ligh...