Search found 2585 matches

by Hobbyst46
Tue Apr 21, 2020 3:43 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 794

Re: Imaging with different coverglass thickness

Thank you for the warm welcome everyone. I have taken everyone's comments into consideration and it is much appreciated. I recently had to learn how to set up calcium imaging in the institute and I'm having trouble figuring out the minutia of the system. Perhaps to elaborate more on my question. I ...
by Hobbyst46
Tue Apr 21, 2020 11:13 am
Forum: Pictures and Videos
Topic: Mustard Seeds (First attempt at focus stacking - feedback appreciated!)
Replies: 5
Views: 437

Re: Mustard Seeds (First attempt at focus stacking - feedback appreciated!)

...Absolutely love the size of the M50 and the fact that I can use it for non-microscopy purposes, though I'm a little undecided on how it performs as a microscope camera. I control it remotely through the EOS Utility software either on my phone or PC. The only downside with that is there's a bit o...
by Hobbyst46
Tue Apr 21, 2020 9:32 am
Forum: Pictures and Videos
Topic: Mustard Seeds (First attempt at focus stacking - feedback appreciated!)
Replies: 5
Views: 437

Re: Mustard Seeds (First attempt at focus stacking - feedback appreciated!)

Nice picture.
How much do you like the M50 ? is it completely controlled from the Canon EOS Utilities software ? it would be nice also to see single photos of flat thin images.
by Hobbyst46
Mon Apr 20, 2020 9:06 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 794

Re: Imaging with different coverglass thickness

The prices are eye watering. Are they really worth this much? 4-well Chambered Coverglass w/ non-removable wells, 1000 USD for 96 pieces! That means about 10$ apiece. From the point of view of a researcher, who grows cells within the chamber, perhaps under 4 different sets of conditions, and say fo...
by Hobbyst46
Mon Apr 20, 2020 9:00 pm
Forum: Microscopes and optics
Topic: Problems with Omano OM36L
Replies: 5
Views: 384

Re: Problems with Omano OM36L

The first issue is flickering of the LED. The microscope has a wheel style brightness control and at medium to high brightness the LED flickers a lot, to the point where it sometimes goes completely dark. I have checked the batteries and they are producing the correct voltage and the flickering occ...
by Hobbyst46
Mon Apr 20, 2020 8:48 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 794

Re: Imaging with different coverglass thickness

apart from the cover slip thickness the further medium between cover slip and object is part of the calculation.If there is a tiny water layer cover slips of less than 0,17mm might actually offer a better image. These are chambers, live cells grow within, so the water layer is not very thin, but pr...
by Hobbyst46
Mon Apr 20, 2020 7:52 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

Re: More phase contrast photos of cleaned diatoms

Really nice comparison. The second one looks stunning. Btw what mounting media are you guys using? Thanks Wes ! The mounting medium is Pleurax. Some time ago I tried Norland's NOA 61, it is a lot easier to apply than Pleurax but less satisfactory because it does not always penetrate into the frustu...
by Hobbyst46
Mon Apr 20, 2020 6:46 pm
Forum: Microscopy accessories
Topic: 25M female to RMS male
Replies: 6
Views: 480

Re: 25M female to RMS male

Three years ago there was an eBay seller from Spain, named micro_crystal, from whom I purchased spacer RMS adapters to use short barrel objectives together with DIN objectives. He was representing a workshop that produced them. Perhaps he makes/sells such adapter as you seek. He was friendly and com...
by Hobbyst46
Mon Apr 20, 2020 6:18 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

Re: More phase contrast photos of cleaned diatoms

@daruosha: Here is an article about cleaning diatoms with hypochlorite: A Simple, Inexpensive Method for Cleaning Diatoms Author(s): Jennifer M. Carr, Gary L. Hergenrader, Nels H. Troelstrup and Jr. Source: Transactions of the American Microscopical Society, Vol. 105, No. 2 (Apr., 1986),pp. 152-157 ...
by Hobbyst46
Mon Apr 20, 2020 6:06 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 794

Re: Imaging with different coverglass thickness

Here is a conjecture: it is non-uniform nomenclature. 0.17mm-thick coverslips are considered to be ideal for high-NA imaging. This includes fluorescence studies (like the Fura-2). There are several mafg. of "chambered coverslips" (the term is new to me - knew them by other names). For example, Ibidi...
by Hobbyst46
Mon Apr 20, 2020 4:30 pm
Forum: Camera systems and imaging
Topic: Advice on a dark field camera
Replies: 21
Views: 829

Re: Advice on a dark field camera

Congratulations. Waiting to see still images.
Please show the setup of the camera on the microscope. Do you think it can be used in series with an eyepiece ? not a special photo (or projective) but an ordinary eyepiece ? do they have a 0.5X lens to catch a larger field of view ? thnaks in advance.
by Hobbyst46
Mon Apr 20, 2020 3:58 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

Re: More phase contrast photos of cleaned diatoms

Thanks, Bob and darousha. @darousha: Diatoms were cleaned with APS - see this post: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=10&t=8101&p=72044&hilit=APS#p72044 One step of boiling in APS, then, instead of repeating it, I rinsed with distilled water, then applied house-grade hypo...
by Hobbyst46
Mon Apr 20, 2020 1:14 pm
Forum: Pictures and Videos
Topic: Questions about a wounded or devoured mentha leaf I observed
Replies: 4
Views: 283

Re: Questions about a wounded or devoured mentha leaf I observed

The 25X eyepieces are useful with the 4X and 10X objectives only. The 40X and 100X will give "empty magnification" that is, no further resolution. So the X2500 magnification is sales promotion but not a real virtue. The 10X eyepiece can serve for the whole set. Forum member mrsonchus (among others) ...
by Hobbyst46
Mon Apr 20, 2020 12:46 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

Re: More phase contrast photos of cleaned diatoms

Thanks for the reply, Doron I can think of three plausible reasons why Green might be considered optimum [as distinct from maximum] The human eye is most sensitive in the Green region many monochrome cameras are also tuned to that range most colour cameras use the Bayer mosaic, which has twice the ...
by Hobbyst46
Mon Apr 20, 2020 11:33 am
Forum: Pictures and Videos
Topic: Questions about a wounded or devoured mentha leaf I observed
Replies: 4
Views: 283

Re: Questions about a wounded or devoured mentha leaf I observed

\the second and third photos show the bottom (?) epidermis of the leaf. The "skin".
To get the whole leaf in focus - depends on the objective. Please post more details about your microscope.
by Hobbyst46
Mon Apr 20, 2020 11:30 am
Forum: Introduce yourself
Topic: Hello everyone.
Replies: 6
Views: 641

Re: Hello everyone.

Hello fellow countryman, and welcome.
by Hobbyst46
Mon Apr 20, 2020 9:35 am
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 1029

Re: Maybe a way to get the perfect image with a DSLR?

Thanks for the photo. Does the 28mm bore socket in the gray-colored stand receive a dovetail, a thread, or is it just smooth ? so the 5mm locking screw presses onto a round surface ?
by Hobbyst46
Mon Apr 20, 2020 9:11 am
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

Re: More phase contrast photos of cleaned diatoms

In principle, best resolution is expected under green light; here, the purpose was just aesthetics. Hope it was achieved. It certainly was achieved ! I’m a little puzzled by your ‘green light’ comment though ... would blue light not give better resolution? MichaelG. Thanks, Michael! In theory, blue...
by Hobbyst46
Mon Apr 20, 2020 9:05 am
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 1029

Re: Maybe a way to get the perfect image with a DSLR?

To be more explicit, my microscope is an Optika IM-3: https://www.optikamicroscopes.com/optikamicroscopes/product/im-3/ and in order to take pictures from a DSLR the following accessories are available: an extension tube https://www.optikamicroscopes.com/optikamicroscopes/product/m-699/ and an opti...
by Hobbyst46
Sun Apr 19, 2020 9:58 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 3762

Re: 40x SPlan woes

Hobbyst46: yes, I did clean the photo projection eyepiece after making the videos (that is were the dirt was). As I mentioned, on April 28th, I get my calibration slide. Please let me know what test I could do to prove the optimal resolution of my equipment. And I will do a bright field still image...
by Hobbyst46
Sun Apr 19, 2020 9:47 pm
Forum: Beginner's corner
Topic: Also Needing Microscope Purchase Advice
Replies: 35
Views: 1627

Re: Also Needing Microscope Purchase Advice

75RR wrote:
Sun Apr 19, 2020 9:40 pm
Has no one noticed the goodies on the microscope I linked to?
I certainly did, but they do not ship to my country, and I cannot afford that sum...
by Hobbyst46
Sun Apr 19, 2020 9:46 pm
Forum: Beginner's corner
Topic: Also Needing Microscope Purchase Advice
Replies: 35
Views: 1627

Re: Also Needing Microscope Purchase Advice

Aren't the plan infinity objectives on the amscope still better than the regular objectives on the zeiss? Or wait what are the objectives on that zeiss? Plan-Neofluar? So it does have good plan objectives? I don't understand why these are so cheap aren't good brand plan objectives quite expensive e...
by Hobbyst46
Sun Apr 19, 2020 8:51 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 823

More phase contrast photos of cleaned diatoms

This is a continuation of http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=10&t=9079 Further slide surfing revealed a Cyclotella meneghiniana Kutzing (tentative idn.). I was very happy since the Centrales are a rare find. So here are images, one with a 40X0.75 Neofluar (dry), the other ...
by Hobbyst46
Sun Apr 19, 2020 7:21 pm
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 53
Views: 3762

Re: 40x SPlan woes

My experience with a BH-2 is that it is a superb microscope and that SPLANS (at least the 10X) are great objectives. Having looked at the various videos from top to bottom, my personal feeling is that nothing is wrong with the microscope or objective. The illumination seems less than optimal. Two mu...
by Hobbyst46
Sun Apr 19, 2020 1:45 pm
Forum: Beginner's corner
Topic: Also Needing Microscope Purchase Advice
Replies: 35
Views: 1627

Re: Also Needing Microscope Purchase Advice

Hello and welcome! I would start with a trinocular microscope right ahead and not build on getting a trino later. I would also start with a microscope that has Kohler illumination and is expandable at least to phase contrast and darkfield. Especially, given the specimens you like to observe. Of the ...
by Hobbyst46
Sun Apr 19, 2020 11:32 am
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 1029

Re: Maybe a way to get the perfect image with a DSLR?

Thanks for the detailed answers, and for the idea of extender.
by Hobbyst46
Sun Apr 19, 2020 9:25 am
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 1029

Re: Maybe a way to get the perfect image with a DSLR?

There are two main factors creating these deformations, IMHO... The lengthy tube, even manufactured with care, cannot ensure the top lenses are exactly on the optical axis. Any small misalignment, even with a very small angle creates the purple-yellow fringes. I got this effect on my microscope, an...