Search found 2585 matches

by Hobbyst46
Sun May 10, 2020 7:48 pm
Forum: Camera systems and imaging
Topic: Images from LabCam and LabCam Pro
Replies: 2
Views: 215

Re: Images from LabCam and LabCam Pro

I refer only to images of (as far as I can guess) a thin flat specimen, like the two top images, of a reddish-purple stained cell tissue. Those should be coast-to-coast in focus. However, in my opinion, sharpness is fair in the middle and less so in the periphery; that could result from the camera a...
by Hobbyst46
Sun May 10, 2020 7:29 am
Forum: Pictures and Videos
Topic: Dandelion pappus ("parachute") details
Replies: 5
Views: 426

Re: Dandelion pappus ("parachute") details

Very beautiful.
Thanks for the link - very interesting reading material !
The pappus summons photography from various angles, including epi-dark field, which might reveal the longitudinal "folds".
by Hobbyst46
Sat May 09, 2020 11:47 am
Forum: Illumination Techniques
Topic: Flashlight as permanent illuminator inside the base of the microscope ?
Replies: 10
Views: 623

Flashlight as permanent illuminator inside the base of the microscope ?

The idea of using a flashlight as illuminator for the microscope has flashed more than once on the forum, and seems an attractive inexpensive alternative. Just before the current corona era I purchased a 120mm by 28mm (widest diameter) rechargeable single-die LED flashlight. It is made of metal, foc...
by Hobbyst46
Fri May 08, 2020 7:31 pm
Forum: Microscopes and optics
Topic: Olympus CX31 stuck objective
Replies: 9
Views: 455

Re: Olympus CX31 stuck objective

A relatively safe approach is cooling the objective then trying to unscrew it. If you can wrap it up with a cold bag of some sort, or even just a damp cloth right out of the freezer, then try to unscrew it before the cold spreads to the turret. The idea is a slight shrink of the male thread.
by Hobbyst46
Fri May 08, 2020 7:22 pm
Forum: Beginner's corner
Topic: Stereo microscope magnification comparison?
Replies: 21
Views: 877

Re: Stereo microscope magnification comparison?

Just leisurely paging through eBay reveals several used AO stereo microscopes, relatively inexpensive, some are bids and some - Make Offer. Just an example: https://www.ebay.com/itm/AMERICAN-OPTICAL-569-Stereo-Zoom-Microscope-4-sets-of-eyes-External-Light-Kit/233443505920?hash=item365a513700:g:0vYAA...
by Hobbyst46
Fri May 08, 2020 1:43 pm
Forum: Camera systems and imaging
Topic: Question about eyepiece camera thru camera port
Replies: 6
Views: 381

Re: Question about eyepiece camera thru camera port

Thanks for the replies. I know the camera will get only part of the FOV, my question was related to the focus. Will the camera be in focus at the same time as the eyepieces or if I focus the eyepieces then I need to refocus if I will use the camera? Thanks again! The camera tube is inserted into th...
by Hobbyst46
Fri May 08, 2020 8:03 am
Forum: Camera systems and imaging
Topic: Question about eyepiece camera thru camera port
Replies: 6
Views: 381

Re: Question about eyepiece camera thru camera port

My inexpensive eyepiece camera, which includes a 0.5X reducing lens, only catches the center of the FOV. Here is a detailed test that I performed:
viewtopic.php?f=9&t=7145&p=63335&hilit=micam#p63185
by Hobbyst46
Thu May 07, 2020 2:42 pm
Forum: Microscopes and optics
Topic: Microscopes in the Movies
Replies: 6
Views: 486

Re: Microscopes in the Movies

Please see this post for a similar inquiry:

viewtopic.php?f=26&t=6737&p=60322&hilit=movies#p60205
by Hobbyst46
Thu May 07, 2020 1:25 pm
Forum: Microscopy accessories
Topic: Power supply woes
Replies: 17
Views: 588

Re: Power supply woes

Sorry for not being able to provide specific info. Ancient capacitors often deteriorate with age. Oldish cloth-style wire insulation could fail as well. My Carl Zeiss power supply (for the 6V 15W tungsten lamp) was repaired for free by an electronics technician, but after a while failed again. Moder...
by Hobbyst46
Tue May 05, 2020 8:29 pm
Forum: Beginner's corner
Topic: first microscope
Replies: 18
Views: 1399

Re: first microscope

Great !
Hopefully other folks, who are uncertain about which microscope, stereo or compound, should serve best for kids, will similarly prefer a stereo microscope. It is so much easier for youngsters.
by Hobbyst46
Tue May 05, 2020 6:55 pm
Forum: Beginner's corner
Topic: Where to place filters in light path?
Replies: 9
Views: 506

Re: Where to place filters in light path?

Deep blue filters...You can fit it over your light source. It doesn't matter much where it is if it's clean . If I place a glass filter directly on the glass cover of the field aperture (the beam port in the base of the microscope), for example, and even if it is not scrupulousely clean, it remains...
by Hobbyst46
Tue May 05, 2020 9:27 am
Forum: Microscopy accessories
Topic: The most important microscope accessory
Replies: 11
Views: 1323

Re: The most important microscope accessory

Clipboard02.jpg Please, some more details about the setup on the right side of the lab, within the yellow frames: Is that a Zeiss standard microscope? Which camera is on top ? which adapter ? which photo eyepiece if any ? How do the images from the camera compare with the (very beautiful) photos in...
by Hobbyst46
Mon May 04, 2020 2:43 pm
Forum: Pictures and Videos
Topic: Actinocyclus subtilis
Replies: 18
Views: 812

Re: Actinocyclus subtilis

Superb !
April-May are the best for seeweed on this side of the sea, yet the beaches are still closed...
by Hobbyst46
Mon May 04, 2020 10:47 am
Forum: Illumination Techniques
Topic: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp
Replies: 22
Views: 1243

Re: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp

In a very recent post by Charles Krebs, diffuse illumination around the objective, which I think can be realised with relatively simple and available means, was presented - along with stunning images.
by Hobbyst46
Sun May 03, 2020 8:11 pm
Forum: Beginner's corner
Topic: WHAT CAN I DO????????
Replies: 9
Views: 627

Re: WHAT CAN I DO????????

Perhaps, if you store small sample jars in a 1 Qt or so LockLock container in the fridge, your family will accept it. Anyway, it is impossible to store live organisms in a static status. The organism is under constant development, cells divide and other cells die; some folks, on this forum, describe...
by Hobbyst46
Sun May 03, 2020 7:10 pm
Forum: Beginner's corner
Topic: WHAT CAN I DO????????
Replies: 9
Views: 627

Re: WHAT CAN I DO????????

Shirley, live matter, for example proteins, consists of compounds of nitrogen, sulfur, and other elements. In the infusion, bacteria start to multiply and decompose the organic matter. The products of decomposition are, unfortunately, smelly vapors and gases, a mixture of ammonia, hydrogen sulfide a...
by Hobbyst46
Sun May 03, 2020 4:16 pm
Forum: Camera systems and imaging
Topic: Comparison Images/Videos | $8 Phone Adapter | $110 NDPL Adapter | $450 NDPL Adapter
Replies: 7
Views: 837

Re: Comparison Images/Videos | $8 Phone Adapter | $110 NDPL Adapter | $450 NDPL Adapter

- What other photographic methods could I try to improve image & video quality? - Would using a photo-eyepiece & photo-tube likely produce better images than the adapters used? - Is a compatible photo-tube even be available for the AmScope T-490B? I'm unsure on how it would mount - Would a dedicate...
by Hobbyst46
Sun May 03, 2020 8:16 am
Forum: Microscopy accessories
Topic: Canon M50 limitations
Replies: 21
Views: 957

Re: Canon M50 limitations

Does anyone know if the Canon EOS Utilities, when in full control of a tethered camera, enables: 1. the Magnification frame (X5, X10) 2. the self-timer delay time on the M50 (or other Canon that has these features) Thanks in advance. Hi, the answer to both 1 & 2 is yes - I use Canon Utilities v3 wi...
by Hobbyst46
Sun May 03, 2020 6:24 am
Forum: Microscopy accessories
Topic: Canon M50 limitations
Replies: 21
Views: 957

Re: Canon M50 limitations

Does anyone know if the Canon EOS Utilities, when in full control of a tethered camera, enables:
1. the Magnification frame (X5, X10)
2. the self-timer delay time
on the M50 (or other Canon that has these features)
Thanks in advance.
by Hobbyst46
Sat May 02, 2020 9:12 pm
Forum: Microscopes and optics
Topic: Zeiss WL Modified with Tilt Top
Replies: 20
Views: 1900

Re: Zeiss WL Modified with Tilt Top

Magnificent !
Now that it is visible, tilting looks like a very attractive feature, that is missing in most microscopes... ;)
I wonder if focusing can be locked, such that tilting back and forth does not move the head out of focus.
by Hobbyst46
Sat May 02, 2020 9:01 pm
Forum: Microscopy accessories
Topic: Canon M50 limitations
Replies: 21
Views: 957

Re: Canon M50 limitations

This is extremely weird !! When Canon started the EOM-M mirrorless line of cameras, one feature of DSLRs they dropped was direct tethering, via cable to a computer. Many people were unaware of it and were surprised to discover it later. So, the EOS-M, M3, M6, M10 (like mine) and M100 can be remote c...
by Hobbyst46
Sat May 02, 2020 6:38 pm
Forum: Introduce yourself
Topic: Cheers
Replies: 1
Views: 342

Re: Cheers

Hi all, I’ve been running reef tanks (salt water aquarium with coral) for nearly 2 decades. I recently decided that I wanted to have a better idea of what was going in there. To the naked eye dinoflagellates, diatoms, cyanobacteria, and phytoplankton don’t always look very different, but of course ...
by Hobbyst46
Fri May 01, 2020 10:23 pm
Forum: Camera systems and imaging
Topic: Help needed to design adaptor for Mirrorless camera
Replies: 13
Views: 699

Re: Help needed to design afocal adaptor for Mirrorless camera

@Marco68
From the scheme, it looks like you are designing a projection adapter, not afocal as the post title shows. Afocal means that there will be a camera lens between the camera and the eyepiece of the microscope, not just the camera body.
by Hobbyst46
Fri May 01, 2020 6:57 am
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 2927

Re: Specimen ethics

When I'm done with a slide, everything goes into a bucket filled with IPA. After a week or two in the bucket I pour off the alcohol and toss the slides/coverslips in the trash. I would avoid having any container of IPA that is not hermetically closed. Alcohol vapors are not toxic but are harmful. S...
by Hobbyst46
Fri May 01, 2020 6:35 am
Forum: Resources (online, books etc.)
Topic: Is it possible to observe cell wall synthesis under microscope
Replies: 2
Views: 616

Re: Is it possible to observe cell wall synthesis under microscope

ZHR wrote:
Thu Apr 30, 2020 9:49 pm
I assume it's really difficult but I'm interested to see photos or videos of this happen.
Thanks.
Zohar, perhaps this will serve as lead
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6287495/
by Hobbyst46
Thu Apr 30, 2020 8:35 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 2927

Re: Specimen ethics

When I'm done with a slide, everything goes into a bucket filled with IPA. After a week or two in the bucket I pour off the alcohol and toss the slides/coverslips in the trash. I would avoid having any container of IPA that is not hermetically closed. Alcohol vapors are not toxic but are harmful. S...
by Hobbyst46
Wed Apr 29, 2020 8:58 pm
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 12
Views: 1379

Re: Epi-Fluorescence Microscopy and Deconvolution

... RobBerdan, which "blind deconvolution" plug-in or other tool do you suggest for the ImageJ ? I use ImageJ 1.52, and failed to find a deconvolution analysis tool, in my package, having never used this algorithm in the past. Thanks RobBerdan. I will try to deconvolute images that I have collected...
by Hobbyst46
Wed Apr 29, 2020 1:11 pm
Forum: Pictures and Videos
Topic: Micro stonemasons. Difflugia.
Replies: 21
Views: 1642

Re: Micro stonemasons. Difflugia.

Thanks, Charles, for the details.
by Hobbyst46
Wed Apr 29, 2020 7:32 am
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1491

Re: Greetings from Frankfurt

MicroBob wrote:
Wed Apr 29, 2020 5:56 am
It might just be possible to adapt the PZO DIC condenser to the GFL but it won't be easy.
Thanks Bob. Important info!
by Hobbyst46
Tue Apr 28, 2020 8:14 pm
Forum: Introduce yourself
Topic: Greetings from Frankfurt
Replies: 28
Views: 1491

Re: Greetings from Frankfurt

Wes wrote:
Tue Apr 28, 2020 7:06 pm
something I've always wanted to but never got a real chance of trying was to set up DIC on the Junior. The PZO DIC system (they don't call it DIC but thats what it is) is almost certainly going to work (its extremely adaptable).
Would you suggest that the PZO DIC could work on the Zeiss GFL ?