MicrobeHunter.com Microscopy Forum

So i changed my adapter set up, bringing back the tube and attaching the adapter at the bottom of the photo tube. This reduced the empty magnification and, with the blue light filter, made a more pleasant image with some touching up. Is it common for a filter to increase image exposure time dramatic...

Ah i did not consider empty magnification, i will look more into that. And I totally forgot to mention it, but it was an 43x objective with a 10x eyepiece acting as a projecting lens

So here is some more pictures with some differing settings The original picture was taken at 0"6 shutter speed and 100 ISO with Kholer illumination and in Bright field. https://i.imgur.com/Tt1QCPL.jpg Next i toyed around in Photo Pos Pro and got this as a result: https://i.imgur.com/aTrD4vz.jpg I ne...

Well the issue is just mainly balancing shutter speed and iso, however i adjusted the exposer compensation on the camera just to experiment and it worked, however the pictures are still grainy. I’ll post some soon

Some are in phase, however most are brightfield. As for the RAW file i would love to send one once i get home. I’ll use something more organized such as onion epidermis if this is better than trying to photograph rotifers.

Wow this is great! It seems i have a lot to do! I appreciate all the helpful tips and i love seeing people mess with the images haha. I believe a better light source and possibly figuring out the adapter set up with diatoms will be my next step. In the mean time I’ll try my hand at photo editing. I ...

Ah that’s interesting! I’ll keep practicing, and in the mean time look for a diatom test slide, i think this can help me fine tune a set up.
Edit: the only issue is sourcing one

No i do not at all. This too looks better than the original, however it is still lacking crispness. Maybe at this point the objectives do matter? That image was shot at 43x, so resolution should already be low correct? If so would, for example, an APO objective perform remarkably better?

The series 4 trinocular, at least the one i have, has a push/pull lever that lets me direct the light either for my binoculars or for my photo tube.

no it does not, i actually suspected that it might be this and took out the only lens in the adapter that connects the minolta microscope adapter to the canon body. It actually made images sharper and a tad brighter, but that is it. This is why i am at a loss :lol: However it is fun to experiment! T...

75RR wrote: ↑

Tue Jun 23, 2020 6:56 pm

I would suggest that you fine tune your setup with a prepared diatom slide.

There are too many variables in a wet slide to do this properly.

This was what i was thinking. I just need to purchase one. Do you have a recommendation?

DonSchaeffer wrote: ↑

Tue Jun 23, 2020 6:54 pm

Forgive me. I copied your image and played a little with my processing program. otherguysimage1.jpg

I do not mind at all! May i ask what you use? This already looks much better than the original imo!

So with this compensation what else should i do to increase image resolution? The live view shows a much sharper image than the actual photos being produced. So i am at a loss at this moment. I thought it may be the phase objectives but when replaced with a normal 43x, the image is nearly the same.

Now I can see your pictures! -Your camera is just fine, you should set it to silent mode and release from live view to have a shock free release. - Is you camera parfocal to the eyepieces? - Is your water layer as thin as possible? - Is your condenser aperture and height set right? - What eyposure ...

Strange....they are visible to me. Let me fiddle around

edit: i think i fixed the issue, i hope it works!

one additional image i forgot

[/size]Hello all! I recently arrived back home from a family trip, and my first thought was to play with my new stand! It is a trinocular AO Series 4 stand set up for phase contrast. With it I purchased a Minolta microscope adapter and a Canon 40D. The setup is pictured below. https://i.imgur.com/Ec...

Ah thank you for the insight. Maybe some experiments with the cleaning process are in order for a safer method haha. Thank you for all your help!

How “unsafe” do you think this process would be? I am under the impressions it can be done safely at home with the right precautions. Then again i am usually one to look on the bright aide of issues haha. And correct me if i am wrong but would alcohol and acetone not be suitable to dissolve the stor...

On a related note, i found a helpful article with my endeavors in diatoms http://www.microscopy-uk.org.uk/diatomist/rbm_US_Royal.pdf While browsing the mountant chapter, i noticed that out of most, if not all, of the possible mountants, Storax seems the easiest and safest to produce in a home lab. I...

Could the UV hardening resin be used as a mountant?

Hi, I would suggest to contact Michel from Diatoms.nl and ask when Pleurax is available again. The cooking of Pleurax is not that difficult so he probably just has to find the time to cook a new batch. His website changed a lot in recent time so the Pleurax might just have been lost in a digital wa...

Other than dissolving the naphrax in the toluene, do I need any special precautions when using it? IE using it outside? I saw it available in North America through this link: https://www.phycotech.com/Products/Product-Details/ProductName/naphrax-mounting-medium-15ml-export-pack If this is my current...

Hello all! I’ve been experimenting with cleaning diatoms with some methods i read during my quarantine induced web browsing. So far I used just household bleach and it seems to work well, I will see if i can prepare a larger sample to clean. My next step is to mount any diatoms i clean, however it s...

I second this! And your mini aquarium is pure genius.

Fantastic images! I would never have guessed they were from a compound microscope.

75RR wrote: ↑

Wed May 20, 2020 1:15 pm

Hi rabela, welcome

I am hoping you have an interest in photographing marine plankton from your exotic location!

I second this! I am sure there are plenty of fun marine samples in Guam.

Hello, I am in the process of upgrading my current stand and i would like to have another stand to use my current one as a "backup". Please PM me if you can point me in the right direction haha. Thank you all and have a great day!

Hey everybody! I am shrinking my collection just a wee bit as i get ready for college. I am selling mainly to just focus on one stand instead of collecting multiple, and improve my skill on that one stand. I am selling my Leitz Laborlux here: https://www.ebay.com/itm/Working-Vintage-Leitz-Microscope...

Well currently the issue is finding a condenser at all! I did not realize that condensers were not compatible with all laborlux stands. Maybe i just need to use patch stops.