MicrobeHunter.com Microscopy Forum

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Amazing images, what technique did you use?

Thank you all for the kind words.

75RR wrote: ↑

Mon Apr 27, 2020 7:15 pm

Do you carry sample jars as a matter of course or did you improvise?

I usually carry a small plastic tube with a screw lid whenever I go to the park. You never know if something intriguing pops up.

I was walking around in the park the other day and couldn't resist the sight of a floating piece of pond scum. Among the very many different things I found in there Paramecium bursaria was one of the most abundant species. I took a short video that demonstrates the movement and a couple of close up ...

Really nice! A very cool protozoan and superb quality, the darkfield is amazing!

https://i.imgur.com/BYZK9Hp.jpg Brilliant, thats really impressive and my favorite shot from your 8-form diatom series. Are there any diatoms from this slide that haven't encountered the 63x planapo? I was never able to get achieve a useful level of oblique illumination but I could try something si...

Re intracellular crystas in Paramecium - nice pictures. I have also photographed them and made movies of their birefringence under polarized light. I was trying to find out more about them and found a link to this discussion. I had read some of the references already mentioned and also that the cry...

Thank you Sinabro!

Looks very nice, the only thing I'd change would be the angle of lighting so we can see more surface details. Where is your light source situated?

Thanks 75' and KD

Thank you Hobbyst46 and daruosha!

Really nice comparison. The second one looks stunning.

Btw what mounting media are you guys using?

40/0,95 apo 2x speed

Cool, I really like the darkfield part!

Yes!

Welcome.

I would be quite interested to see your microscopes, always interesting to see what people are using.

Very nice, that's my favorite diatom to play around with when I want to demonstrate the effect of condenser aperture and wavelength on resolution. You have now documented nearly all of the 8 form test slide with you 63x planapo as far as I remember from your previous threads. Are you planing on shoo...

Great idea Bob! Very interesting approach for studying surfaces of objects otherwise not suitable for transmitted light microscopy.

Thanks KD.

Typical NA for a 40x planapo is 0,95. Keep in mind that for a crystal clear image you want the specimen you're looking at to be as close as possible to the coverslip and you also have to adjust the coverslip correction colar. Otherwise the image will lack contrast and sharpness.

Amazing stuff. I wonder how the cell manages to produce scales of defined geometry.

Found another one. This time the amoeba was at home - like the rest of us. There is just a hint of the scales that the Testa (shell) is made of. rY0vHtwRz-c The elongated structures visible in vacuoles around the 0:35 mark, are these scales ready to be deployed or in process of maturation?? I'll tr...

I second that, great quality and beautiful location you got there.

In a nutshell you dip the prism in immersion oil which crawls inside the gap separating the two quartz wedges.

http://www.microscopeitaly.it/2011/06/2 ... /#more-251

According to the author it is quite a durable hack that last for quite a while.

Amazing stuff. Thanks for posting, in fact this got me inspired to go the local canal and take a bit of brown scum which was chock full of diatoms.

75RR wrote: ↑

Wed Apr 15, 2020 8:25 pm

though there appears to be delamination in at least one of the prisms.

There is an immersion oil hack of fixing delaminated DIC condenser prisms.

Nice microscope!

What objectives does it have and why is there a tube element connecting the binocular head to the tubehead?

Many thanks MichaelG, daruosha and 75RR. I was aware of MagicLantern for years but never knew how it would perform and affect the actual usability of the camera(bugs, etc..). It contains certain risks such as bricking your camera. For example trying to boot the camera without the AUTOEXEC.BIN file c...

75RR wrote: ↑

Wed Apr 15, 2020 6:56 pm

The nosepiece is from a Universal/Photomicroscope but the slider holders can be taken off and put on a Standard or WL

I thought they are stuck to the nosepiece. Factory aligned and glued in place.

So far I've only been able to take photos with my Canon 50D. Finally I got around to expanding the Canon firmware with Magic Lantern which now allows me to shoot 1080p video at 30fps. Once I have the have the video files on my computer I use ffmpeg for transcoding and editing. Here I present one of ...