MicrobeHunter.com Microscopy Forum

As a side note I've recently been using UV-LOCA TP-2500F Glue for slide mounting subjects. This is a UV curing glue used for mounting screen covers on mobile phones and tablets. So far it is working very well. It doesn't harden fully so care needs to be taken with the amount used to avoid flooding o...

A while ago I bought a few high power Cree LED's and tested them while refurbishing a Vickers M17 microscope. I found the Cree XHP35 to have the most even phosphorous distribution and light pattern. Its a single die 12v LED with a die size of 3.45 x 3.45mm which closely matched the original filament...

The GS logo is 'Gillette and Siebert'. They were a UK company who produced good quality microscopes up until the late 80's. Their microscopes were mainly assembled from outsourced EU production parts. I have one of their scopes and the objectives are achromats like your example. I'm sure someone her...

Three to get you started:
http://www.microscopy-uk.org.uk/pond/
http://www.plingfactory.de/Science/Atla ... ista1.html
http://www.plingfactory.de/Science/Atla ... ifera.html

Very nice, well done.Was the specimen mounted or just a wet slide?

Fantastic images.

Lovely video, well done.

Scarodactyl, I don't use it always but it is useful sometimes for positioning a moving object (eyepiece is widefield, image is cropped), checking focus when adjusting a 40x collar, that sort of thing. I like to be able to glance up from the eyepiece and see what the camera is seeing. Michael I sugg...

I ordered an M50 2 days ago and awaiting arrival. I did my research and understood the camera can be triggered via EOS Utility 3 and USB tethering (as geo_man has working). My ageing 550D works flawlessly this way. The loss of a second trigger method isn't a concern, but I will be annoyed if Canon's...

Try this link (UK eBay, but should ship to Spain as its a Chinese seller): https://www.ebay.co.uk/itm/AC-Power-Ada ... SwBBpbrPXA

eBay item number 323470694306
HTH.

Hard to be certain, but looks like Colpodea.

Chris... I got the 90D because I want a higher resolution APS-C; a fully electronic shutter from live-view; and I wanted a camera that could do a quick focus stack sequence when used outdoors or for lower magnification close-ups. It does all three. Like all other cameras it is not "perfect", but th...

Well done Wes, another stunner.

Well done Karl, lovely to watch. Congrats on finding a complete colony to study, I've yet to find one. Thanks for sharing.

Great images and subject treatment. Are you happy with the Canon 90D? I'm considering buying one, any downsides?

Thanks for the kind words all. The critter tank has seen a rise in numbers with the longer daylight hours.

I just saw that you use a Vickers microscope! I have a good impression from them but very few poeple seem to actually use one. Bob Thanks for the reply. I'm really enjoying the Vickers M17 Bob, well worth the effort restoring it. Very heavy, no plastic gears, and a generous field of view. Despite h...

The shape of that test is very unusual. Is this fossilised jimur?

Thanks for the info' daruosha, should have known there would be a name for it. This is similar to the examples shown in the document, though in this case the offset illumination is well outside the phase ring of the 16x objective. The attached pic is a crude shot down the eyetube (no phase telescope...

I was experimenting with 3D printed patch stops and stumbled upon this illumination effect. I'm sure this is not new to the more experienced members here but I found it interesting. I'm certain this is the closest I'll ever get to real DIC :D This might be specific to my setup, but here's how I got ...

The lengths these ciliates will go to (literally) in order to hunt is remarkable.



Microscope: Vickers M17 trinocular. LED illumination.
Objectives: Zeiss Fluotar 16x & Zeiss Jena 6.3x APO
Illumination: Bright-field and Dark-field
Phototube optics: Olympus NFK 3.3x
Camera: Canon 550d

Nice work KD. Chunky fella, fun to watch. Thanks for sharing.

Dead, or do Heliozoa have dormancy periods? Watched for some time but no axopodia visible.
I'm assuming this isn't something else?

A better shot of the Coleochaete algae thallus that I'm finding in the tank.

MicroBob wrote: ↑

Fri Apr 17, 2020 8:33 am

Are you using the right eyepiece for the objective?

None of the eyepieces I have give a flat field (inc' a pair of Jena PK 6.3x). Not a problem though, I use this objective for video and the crop factor restricts capture to the centre sweet spot.

What a beauty. Congratulations Melifluonze, it must have been a lot of work getting it to this stage. I hope it brings you much enjoyment.

MicroBob wrote: ↑

Fri Apr 17, 2020 5:25 am

The old 33mm Zeiss apos gave a very nice image but had a lot of field curvature.

I can second that. My Zeiss Jena 6.3x Apo is great in the centre through WF eyepieces, but not good outside 60% of the viewable area. Obviously designed for NF eyepieces, even then it would not be considered planar.

Of the high power LED's I've tried some had issues with uneven luminosity due to patchy phosphorous distribution, this is visible through the eyepiece socket when focusing the lamp optics. The best I've found so far is the Cree XHP35, this is a 12v 13W monolithic die. The die size is 3.5x3.5mm and v...

Edit: removed, responded before post completed.