Search found 3154 matches

by MicroBob
Tue Aug 27, 2019 7:17 am
Forum: Microscopes and optics
Topic: Which model of Carl Zeiss Standard is this?
Replies: 49
Views: 15993

Re: Which model of Carl Zeiss Standard is this?

Zeiss West had a bunch of different auxiliary lenses so one should avoid to get them mixed. To illuminate a wide field it might be necessary to lower the condenser. With the right auxiliary lens it is possible to get the field aperture in sharp focus nontheless. The additional auxiliary lens on your...
by MicroBob
Tue Aug 27, 2019 4:46 am
Forum: Microscopes and optics
Topic: Which model of Carl Zeiss Standard is this?
Replies: 49
Views: 15993

Re: Which model of Carl Zeiss Standard is this?

Hi Michael, I don't know the WL but my Phomi from the 1960s has two field apertures, one below the condenser it the foot, one just before the bulb in the back of the foot. May be your two lenses are meant to allow focussing of the condenser on two different apertures or to focus with differentconden...
by MicroBob
Mon Aug 26, 2019 7:50 pm
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 132
Views: 108693

Re: Pollen. There's lots of it about.

Hi Alan,
a nicely even cut!

I applied some more unsharp masking to your image - a matter of personal preference, to me it looks a bit more clear.

Bob
by MicroBob
Mon Aug 26, 2019 7:38 pm
Forum: My microscope
Topic: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope
Replies: 14
Views: 7177

Re: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope

...when I get back from our schrabergarten. Hi Alan, isn't this the perfect reason for a field microscope? :D The image is very sharp in the middle and there isn't much chromatic aberration. But the barcode also look much like a potato chip! Michaels optical system would be perfect: A lens with lon...
by MicroBob
Mon Aug 26, 2019 7:36 pm
Forum: Microscopes and optics
Topic: Microscope Upgrade
Replies: 19
Views: 8274

Re: Microscope Upgrade

"Plan" is used very differntly: Zeiss 160mm Plan was calculated for a flat field of 25mm. Leitz Plan was 28mm, NPL was 24mm. Your Bresser eyepieces have probably 18mm field diameter and a chinese "Plan" might not fully cover it. The halogen lamp should have a filter in front that blocks the UV light...
by MicroBob
Mon Aug 26, 2019 7:27 pm
Forum: Microscopy accessories
Topic: Standard Plan Objectives v Plan Infinity objectives.
Replies: 11
Views: 7028

Re: Standard Plan Objectives v Plan Infinity objectives.

Hi Dave, an expensive Plan objective like the old Zeiss 160mm ones will probably have had a generally better correction than the very much cheaper basic achromats. But for plancton objects both are usable. For 160mm Objective you need the right eyepieces as these correct remaining colour errors and ...
by MicroBob
Mon Aug 26, 2019 7:16 pm
Forum: Camera systems and imaging
Topic: what camera lens to use for Parphocal setup?
Replies: 19
Views: 9902

Re: what camera lens to use for Parphocal setup?

40mm is right for normal field sizes like 18mm or so. For something really wide like 28mm a shorter focal length might be better. A proven lens for this purpose is the Pentax M 40mm 2,8 pancake from the late 1970s. The idea is that the rectangular sensor just fits into the roung field, no black corn...
by MicroBob
Mon Aug 26, 2019 7:08 pm
Forum: Microscopes and optics
Topic: measuring reticle for my eyepiece
Replies: 7
Views: 3219

Re: measuring reticle for my eyepiece

From other, mostly simpler eyepieces I can remember tht there often was a stop inside that just was a perfect place for a reticule. I had to transform most on my eyepieces into measuring eyepieces for a group meeting a couple of years ago. A basic reticule can be printed on OHP foil with a laser pri...
by MicroBob
Mon Aug 26, 2019 7:04 pm
Forum: My microscope
Topic: Just arrived in the mail from Russia
Replies: 10
Views: 7499

Re: Just arrived in the mail from Russia

It doesn't really matter any more since the content was destroyed when it was hit with the post office stamp. Now proove that I'm wrong, Eddie!

Bob
by MicroBob
Mon Aug 26, 2019 2:19 pm
Forum: My microscope
Topic: 1917 Spencer, Monocular Olympus KHC, Siebert EmoSkop, and Swift M950
Replies: 10
Views: 6761

Re: 1917 Spencer, Monocular Olympus KHC, Siebert EmoSkop, and Swift M950

Hi, welcome to the forum! You have already a nice selection of instruments there. The condenser of the Olympus is probably held in a sleeve of a certain diameter. When you measure the diameter with a caliper somebody in your area may be able to offer you a suitable condenser. The condenser forms a l...
by MicroBob
Sun Aug 25, 2019 9:16 pm
Forum: My microscope
Topic: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope
Replies: 14
Views: 7177

Re: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope

Hi Michael,
that is exactly the principle, only in stereo version.
It only really makes sense for a binocular maufacturer to offer such an add-on and the groups of buyers of Zeiss binoculars and stereo microscopes probably don't overlap much. But the result is much better than one would expect.

Bob
by MicroBob
Sun Aug 25, 2019 7:43 pm
Forum: Pictures and Videos
Topic: Sand samples
Replies: 2
Views: 1603

Re: Sand samples

I'm sure you are right 75, I had lots of them in my plancton aquarium.
Interisting life form with the legs operating through a slit in the shields.
If theye were humans the would the perfect customer for an insurance salesman.

Bob
by MicroBob
Sun Aug 25, 2019 7:39 pm
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 132
Views: 108693

Re: Pollen. There's lots of it about.

Hi Alan,
you have found a very attractive way to present your pollen series! I like the combination of pollen image, plant image and short explanation all in one picture a lot.

Bob
by MicroBob
Sun Aug 25, 2019 7:29 pm
Forum: Pictures and Videos
Topic: Desmidiacea images - Tharandter wood, Saxonia, Germany
Replies: 20
Views: 6512

Re: Desmidiacea images - Tharandter wood, Saxonia, Germany

Hi together,
here is a video of this interesting ciliate that shows more of it's behaviour. I have never seen anything like it before, but is is common in the water it is from.
https://vimeo.com/355850812

Bob
by MicroBob
Sun Aug 25, 2019 7:26 pm
Forum: My microscope
Topic: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope
Replies: 14
Views: 7177

Re: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope

Hi Alan, this looks very usable, especially for objects of the size one would use a stereo microscope for. And much more compact than the solution I showed here. Expensive smartphones have such good cameras and displays today that it really is a question whether a field microscope should not be set ...
by MicroBob
Sun Aug 25, 2019 5:34 pm
Forum: My microscope
Topic: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope
Replies: 14
Views: 7177

Re: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope

There are two prisms in the housing and below two lenses, probably cemented elements. So you look straight through the binolurar and in the "Stereo Basis" the view is directed inwards to a common spot. The surgeon microscopes I have seen in auctions looked like normal stereo microscopes to me. Usual...
by MicroBob
Sun Aug 25, 2019 4:51 pm
Forum: My microscope
Topic: Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope
Replies: 14
Views: 7177

Zeiss Stereo Basis + pocket binocular - a portable stereo field microscope

Hi together, I recently bought an intersting stereo microscope solution for field use: The Zeiss Stereo Basis + Zeiss 8 x 20 B pocket binocular, from around 1980. It offers 16x combined mag., a good field of view, good image quality and a high eyepoint. Thumb screws have been added by the previos ow...
by MicroBob
Sun Aug 25, 2019 6:20 am
Forum: Microscopes and optics
Topic: Nikon Fluor objectives on a Leitz stand
Replies: 36
Views: 16667

Re: Nikon Fluor objectives on a Leitz stand

based on the eyepiece correction charts Bob linked to Hi Pete, I'm sure that the measurements that are shown in these diagrams are correct. But I would be hesitant to say that they present all there is to know about the CVD of five microscope companies. Apochronaut-Phil for instance had found diffe...
by MicroBob
Sat Aug 24, 2019 8:03 pm
Forum: Pictures and Videos
Topic: Desmidiacea images - Tharandter wood, Saxonia, Germany
Replies: 20
Views: 6512

Re: Desmidiacea images - Tharandter wood, Saxonia, Germany

Hi Heather, that is an interesting report, only i doubt that the relevant persons will read it in time. :evil: In the past there has been a lot of research on algae and other micro organisms in the water, but what has been missing is a continuous monitoring and an alarm level to react in time. So it...
by MicroBob
Sat Aug 24, 2019 7:19 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 24826

Re: Home made (Very Basic) Microtome

Hi Alan, to me these sections look even but a bit thick. One point of improvement might be the surface of the top washer. As it looks it might easily scratch the edge while cutting. I haven't tested them for this purpose but the discs in hard discs are even, flat, have a smooth surface, a hole in th...
by MicroBob
Sat Aug 24, 2019 5:44 am
Forum: Microscopes and optics
Topic: Nikon Fluor objectives on a Leitz stand
Replies: 36
Views: 16667

Re: Nikon Fluor objectives on a Leitz stand

If higher n.a. could bring you to use oil immersion the Leitz NPL Fluotar 50:1 1,0 is very good. Originally intended for use without cover slip the 0,29mm working distance allow to use it with one as long as the preparation is thin. Also interesting is the Zeiss Jena 40:1 0,95 dry Apo in the later 4...
by MicroBob
Fri Aug 23, 2019 8:33 pm
Forum: Pictures and Videos
Topic: NEW: MIKRO Hamburg guest image corner in high resolution
Replies: 7
Views: 3057

Re: NEW: MIKRO Hamburg guest image corner in high resolution

Hi together,
a couple of my recent images can be seen in higher resolution (if possible) on our website now: http://www.mikrohamburg.de/Mikro_Lammert.html
No phase contrast involved here! 8-)

Bob
by MicroBob
Fri Aug 23, 2019 8:29 pm
Forum: Pictures and Videos
Topic: Shape-shifting flagellate
Replies: 11
Views: 3942

Re: Shape-shifting flagellate

Hi Wes,
the animated GIF is a really nice way to show the shape shifting as the individual steps are more obvious than a video running at 25 fps.
For people with a lousy or expensive internet connection it is also easier to access.

Bob
by MicroBob
Fri Aug 23, 2019 8:15 pm
Forum: Pictures and Videos
Topic: Desmidiacea images - Tharandter wood, Saxonia, Germany
Replies: 20
Views: 6512

Re: Desmidiacea images - Tharandter wood, Saxonia, Germany

Hi Wes, this is a built-in feature of Zeiss DIC "alt". There is an analyser slide in the filter slot in the tube head with a thumb screw that moves the prism. At one end one typically starts with light blue, then dark blue, violet, red, orange... In the middle position the imag is colour neutral, so...
by MicroBob
Fri Aug 23, 2019 4:40 pm
Forum: Camera systems and imaging
Topic: Third ocular on camera port of Fluoval mic.
Replies: 19
Views: 8644

Re: Third ocular on camera port of Fluoval mic.

Hi Petro, I think you show already a double photo atattchment and now want to buy a third one - probably a record then. For photography you need a camera and an eyepiece, preferrably parfocal, and a method to get the image into both. This is already done by your trinocular adapter, that can switch t...
by MicroBob
Fri Aug 23, 2019 2:04 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 24826

Re: Home made (Very Basic) Microtome

I found it to be important to choose the right images to stack and leave all other images out. So may be begin 1 image before the first sharp areas appear, then through the stack down to the middle of the object or so, not too deep. In Picolay you can select "align images". I'm not a stacking expert...
by MicroBob
Fri Aug 23, 2019 12:40 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 24826

Re: Home made (Very Basic) Microtome

Hi Alan, this is starting to look really good! For further improvent you might look into stacking. Picolay is free and works well. To my surprise stacking still improves the quality of images of flat sections - the depth of focus is smaller than the slight unevenness of the sections. This will get r...
by MicroBob
Fri Aug 23, 2019 11:41 am
Forum: Pictures and Videos
Topic: Desmidiacea images - Tharandter wood, Saxonia, Germany
Replies: 20
Views: 6512

Re: Desmidiacea images - Tharandter wood, Saxonia, Germany

Hi together,
I stacked the micrasterias images and like the image quality even more than the single image.
We will put a bigger version on our website.

Bob
by MicroBob
Thu Aug 22, 2019 7:53 pm
Forum: Pictures and Videos
Topic: Desmidiacea images - Tharandter wood, Saxonia, Germany
Replies: 20
Views: 6512

Re: Desmidiacea images - Tharandter wood, Saxonia, Germany

Here is a link where an animate GIF shows the duplication of a Micrasterias algae over about 100 minutes. It is from Gerd who sent me the sample.
https://photobucket.com/gallery/user/pl ... Z2lm/?ref=
Just amazing!

Bob
by MicroBob
Thu Aug 22, 2019 7:46 pm
Forum: Pictures and Videos
Topic: Diatoms from the Mediterranean Sea, Israel, alternative cleaning and mounting technique
Replies: 31
Views: 8495

Re: Diatoms from the Mediterranean Sea, Israel, alternative cleaning and mounting technique

Hi Doron, your phase contrast and this slide work very well together, very good contrast and little halo effect. Was this slide mounted in Pleurax or in NOA61? As far as I know phase contrast limits the top resolution slightly due to the size of the phase ring. My DIC system does the same due to the...