MicrobeHunter.com Microscopy Forum

Very nice job indeed Sphaerotilus. As to the fibres, I have to admit the one with the blue appearance looks synthetic, but maybe not. The other, as you say, may be fungal.
Tom W.

Welcome to the forum Jared. I'm sure your expertise will be in demand from time to time. It would be interesting to see some pics of your vintage microscopes in the "My Microscope" forum if that's possible.
Tom W.

Looks very promising Viktor, nice job. Will be very interested to see the results.
Tom W.

Very good results. Well done, and thanks for sharing.
Tom W.

This sounds dumb, even to me, but the description of the residue sound a lot like something "Goo Gone Goo and Adhesive Remover" might deal with. Known ingredients include: PPG-3 METHYL ETHER. Some Concern: developmental/endocrine/reproductive effects. ... KEROSENE (PETROLEUM), HYDROTREATED. Moderate...

Hi farnsy Oddly enough I printed something quite similar a while ago, because I have all the same questions regarding cover slip cleaning and storage as you do, and am still short of answers :( The one I printed differs in that It holds the slides rotated 45 degrees, and of course the really major d...

Haven't read the article yet (it's on my list!) but wonderful work on the images - spectacular.
Tom W.

Nice one Don. Appreciated.
Tom W.

Thank you for the link MicroBob. Looks very interesting. I shall work my way through it when I have a little more time.
Tom W.

mrsonchus wrote: I still use charged slides occasionally but routinely use plain uncharged without subbing either. I find that good sections and clean slides very nearly always give excellent results - adhesion is very rarely a problem. An exception as mentioned may be a particularly inflexible tis...

Thank you for the response re: differentiation of safranin O. That's an impressive array of sections you've produced and the close-ups look really good. There will be a lot of people on the forum interested to see the results after staining I'm sure, and any further information on your experience wi...

Thank you for the clarification mrsonchus. I didn't realize you were not using Johansen's formula for the safranin O. I'm wondering if you have found the addition of the formalin, as mordant, changes the response of the stain during differentiation relative to your normal formulation?
Tom W.

Terrific resource Bob. Thanks very much for posting the link.
Tom W.

More terrific pictures John, thank you! But you've done it now... "enhanced" safranin formula??? Did I miss something? Please elaborate.
Tom W.

Though only slightly...
Tom W

I quite like it. Well done and thanks for posting!
Tom W.

Welcome docjohn from another Ontarian. Yes, great forum, great people, hope you continue to enjoy your microscopical experiences!
Tom W.

Thanks V
And thanks for the link. Interesting web page, even if a little commercial in intent. Wow! Tardigrade T-shirts... I'll have to think about that.
Tom W.

Thanks for this link Bob. An excellent article which could well be considered required reading, and not just for "newbies".
Tom W.

Ah, thank you Tony. Much clearer view of the grooves this time. So the grooves are clearly longitudinal with, it appears in some cases, a slight spiral twist. It would be interesting to see a X-sectional view of such a seta at higher magnification. Might be quite a trick to prepare though! Alternati...

Hi Tony Yes, I see the grooves on the larger setae as you point out, and will take your word for their validity. In the stacked image I found it hard to decide whether I was looking at something really on the setae, or at structures "shining through" from from the various structures behind as a cons...

Yet more very nice images indeed.
Thanks again for sharing.
Tom W.

Excellent! Very informative image - thanks.
I'm assuming this is focus stacked, given the depth of field? There appear to be a few focus stacking type "artifacts" which also suggest this, although I may just be seeing angels on the heads of pins (or the hairs of flies!).
Tom W.

+1 re: all of the above!
Thanks for sharing.
Tom W.

Looks like a useful link Roldorf. Looking forward to checking it out in more detail.
Thanks for posting.
Tom W.

Not just for tardigrade lovers, this is the tardigrade version of a mouse exercise wheel (hint: Volvox). https://www.nature.com/immersive/d41586-021-00095-y/index.html?utm_source=Nature+Briefing&utm_campaign=0da5e44231-briefing-dy-20210209&utm_medium=email&utm_term=0_c9dfd39373-0da5e44231-45417602 S...

Will be following with great interest mrsonchus!
Tom W.

Welcome to the forum GraemeP. If you're in Reading UK, and are not already aware of it, Reading has an active microscopy group - the Reading Microscopical Society. You may want to check it out? If you're in Reading Pennsylvania that may not be of much help
Tom W.

You're very welcome John.
Tom W.

Wow indeed! Thanks for that. Always loved the painting, have the Tracy Chevalier book, seen the movie, and now this. What would Vermeer think?
Tom W.