MicrobeHunter.com Microscopy Forum

Saul

Found time to play with the old project, which was inspired by: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592912/ Initial setup: 1.jpg 2.jpg 3.jpg 4.jpg LEDs from the top & bottom are covered with the aluminum foill (to concentrate the light to the edge of the slide). Of course, there is a dimme...

Saul

Stereo of the same mite (somehow sample "exploded" during the night) , done with the DF adapter for whole slide imaging

: PMN.jpg (76.94 KiB) Viewed 5799 times

Bigger:
https://live.staticflickr.com/65535/501 ... 1e21_o.jpg

Saul

75RR wrote: ↑
Wed Jul 15, 2020 6:24 pm
Striking image!

Had to look up Klein Tools wire lubricant I take it you had it to hand?

Thanks !
I had to add little bit water (too thick) , couple drops on the mite in the concave slide ....

Saul

Unexpected colorful result, playing with different lighting setups - mainly polarized Rheinberg, DF/BF, some extra stuff. Sony A6300, 143 photos, Nikon Microphot with AO modified condenser, lucite Rheinberg filters (ebay, optitec2020), LED/flash illuminator , variable sliding oblique filter in the b...

Saul

MichaelG. wrote: ↑
Fri Jul 10, 2020 10:25 pm
That’s very impressive

I see it raised, but I’m guessing that it’s a cavity [and paired for cross-eyed viewing]

MichaelG.

Thanks Michael, correct !

Reversed version:

: Rev.JPG (280.2 KiB) Viewed 1303 times

Saul

LED diffuser part number imprint stereo, 183 photos, DMap, no name 10x objective (looks like Nikon BD 10x ∞), Microphot with 200mm TL, Pol/Obl

: St D-web910x1024U100.jpg (303.17 KiB) Viewed 1321 times

Saul

I'm assuming that's a resin printed dovetailed cube. I don't know where it goes, but could you fit a 20mm 50/50 non-polarizing beam splitter cube in there? Then you could get one of these to experiment with for $22: https://www.ebay.com/itm/Cube-Beam-Splitter-Prism-Ratio-Optical-Glass-50-50-Spectro...

Saul

DonSchaeffer wrote: ↑
Fri Jun 26, 2020 5:20 pm
What are these for--using a camera?

No , in the microscope as the BF/DF cube for the vertical EPI illuminator, please see this one, or UV version

Saul

microb wrote: ↑
Fri Jun 26, 2020 3:49 pm
What are the dimensions of beam splitter mirror?

Ideal (in my case) - 18mm x 27mm.
Or little bit less

Saul

Looking for the half-mirror (not dichroic) for the Nikon BF/DF DIY cube.

Proof of the concept was made with the SurplusShed dichroic mirror (at the left),working model is at the right, used half-mirror leftover piece.

: _DSC3356-web533x800U100.jpg (59.65 KiB) Viewed 4345 times

Saul

Hi Saul, again a great design! Thank you for sharing your methods so openly! Bob Bob, thank you very much for your words ! More photos http://www.photomacrography.net/forum/userpix/1489__DSC3201web1024x697U100_1.jpg http://www.photomacrography.net/forum/userpix/1489__DSC3194web1024x748U100_1.jpg ht...

Saul

One more model (in parallel to the existing one) - more powerful, swap-able heatsink (let's say you want UV version also), can be used for non flash version, etc. Even more, the same heatsink can be used for both LEDs - reversed to the another side. http://www.photomacrography.net/forum/userpix/1489...

Saul

PeteM wrote: ↑
Sun Jun 14, 2020 11:10 pm
Mine was 32mm sandwiched between glass. Maybe same material. Wouldn't surprise me if your link is the same seller, under a different name.

How it is performing ?

Saul

... but I had always presumed that the tube-lens would be in the upper part of the microscope body ... Hi Michael, correct. I have no plans to purchase the real infinity microscope, but I want to use my infinity objectives from the vertical setup . I tried to use TL on the Microphot top , but it di...

Saul

Scarodactyl wrote: ↑
Thu Jun 11, 2020 11:34 pm
...or is it more an case of sticking with one for a particular set of photos?

Correct. And I have to refocus, what is not a big deal

Saul

Microscope turret solution, using printed slider adapter for the Microphot DIC intermediate tube: http://www.photomacrography.net/forum/userpix/1489_Interm_Micr_slider_1.jpg 100mm - Thor Labs AC254-100-A1 200mm - Reichert tube lens 130mm -130mm FL Achromatic Doublet Lens, 32mm diameter http://www.ph...

Saul

https://www.ebay.com/itm/264308894870
https://www.ebay.com/itm/264513413914

Saul

Thank you all for your kind comments !

Saul

Sony A6300, Mitutoyo5x/125mm tube lens, 305 photos, Zerene DMap, Nikon Microphot http://www.photomacrography.net/forum/userpix/1489_finweb576x1024U100_1.jpg Bigger: https://live.staticflickr.com/65535/49986083771_ec09461e60_o.jpg Stereo: http://www.photomacrography.net/forum/userpix/1489_DMap_stereo...

Saul

For Olympus BH2 (BHS type) microscope
For Olympus BH2 (BHT/BHTU type)

Video : https://youtu.be/UuhMU_Lvnic

Saul

Couple more stereos:

Saul

MichaelG. wrote: ↑
Fri May 15, 2020 10:36 pm
Thank you, kind Sir ... That does it very nicely

MichaelG.

Thanks Michael !
What is strange, I know one person who can see stereo of this reversed stereogram, without stereo glasses or cross-eyeing !
How it is possible ? Brain perception ?

Saul

MichaelG. wrote: ↑
Fri May 15, 2020 8:53 pm

Saul wrote: ↑
Fri May 15, 2020 6:15 pm
Stereo version, done with Zerene, DMap .
Looks very promising, but I must find a quick way of switching these image pairs L-R
... viewed parallel, it looks like a cavity [and I can’t do cross-eyed viewing]

MichaelG.

Saul

Stereo version, done with Zerene, DMap .
Now with the uniform background .

Bigger:
https://live.staticflickr.com/65535/498 ... 5610_o.jpg

Saul

It's really beautiful. Amazing shot. Hoffman type condenser insert/filter set Would you explain it a bit more? Thanks ! Not sure, are you familiar with this conversion insert . " a la Hoffman" extras were used with this set. Instead mirrored surfaces I used pol filters, what gave me ability to adju...

Saul

Sony a6300 , 154 photos, Nikon 50x 0.55 ∞/Reichert TL, Nikon Microphot , Hoffman type condenser insert/filter set, DIATOM LAB di Stefano Barone slide, PS/Picolay/StereoPhotoMaker http://www.photomacrography.net/forum/userpix/1489_Untitled1web568x1024U100_2.jpg Bigger: https://live.staticflickr.com/6...

Saul

AC254-200-A - Ø1.0" , efl=200mm (https://www.thorlabs.com/thorproduct.cfm?partnumber=AC254-200-A-ML) The post said you bought the B coating. But it looks like you more recently bought Thors Labs' actual Nikon tube lens: https://www.thorlabs.com/thorproduct.cfm?partnumber=ITL200 I was hoping the AC2...

Saul

When you say 200mm and 125mm tube lenses, are these achromatic doublets? Or something fancier? 200mm is Reichert Microstar IV head TL (no idea what is inside), 125mm - achromatic doublet. I have a Thorlabs achromatic doublet? Did you ever try that as a tube lens (based on a 2011 posting you had lin...

Saul

... So I'm really interested in understanding the goal here. Do you want to take a Microphot and add the right tube lens to get good images from infinity focus objectives? Hi Ted, it is correct ...Which series would you try? There are the older objectives that require corrections with relay lenses....

Saul

After converting to infinity my Optiphot , it is time for the Microphot. For 200mm I used Reichert TL, works OK, now looking for the shorter range TL . Question - how correctly to setup correct TL FL distance on the microscope, full of internal optics ? I cannot simply take a Microphot to the window...

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