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DIC, 20x, stack of 29

DIC, 20x, stack of 2

Great images! I need to start using my fluorescence equipment as well. Never used it yet...

Thank you all Manfred, you have a point. It is wild life! Still, learning Lightroom is the next thing I'll have to do. I am sure Lightroom has similar features as Elements.

Caterpillar, Butterfly larva tissue section. Sort of boring subject, but I liked the colorful patterns. From prepared slide. 10x, Brightfield.

Water creature with eggs (water flea?) from prepared slide. Dark Field, 10x. No stacking.
I struggled for an hour to clean up the background in Lightroom 5, but I gave up. I need more training...

I like the last one, which almost look like a school book illustration. Looks like a painting almost. Nice effect. Is that achieved in the post processing or a "DIC effect"? I have not experienced such effect on my DIC setup (yet)

Manfred wrote:Great, did you mazerate the speciman (perhaps KOH?), because it`s very clear.

I wish I remembered. This slide was prepared 30 years ago when I had my first microscope. I have then had a 30 year break before I picked up this hobby again.

Wonderful images!

The last one is great! Somehow diatoms look nice on a black background!

Olympus UplanFl40x Phase, however, phase plate was not installed, so it is a regular bright field image.

Another one in DIC, 40x objective (PlanFluorite). 2 images stacked.

This fly needs his legs shaved

Where can one buy Calcofluor? I need some. Great result!

Thanks! Yes it is a great scope with almost endless options and configuration. My goal is to have it complete with all options listed in the brochure. I have collected parts for it for the last two years from all over the world. The confocal option might be out of range. I saw one on ebay, used, but...

My setup Olympus BX51 with fluorescence Epi-illuminator (BX-URA2) Set of UplanSApo (5x, 10x, 20x, 40x, 60x, 100x) Set of phase UplanFl Ph1, 2, 3 (4x, 10x, 20x, 40x, 100x) DIC-prism for the UplanFL objectives Metallurgical (Mplan 1.25x, 2.5x, 10x, 20x, 100x) Phase condenser APO condenser DIC condense...

Thanks everyone. I will try to answer all questions. :) I have an Olympus BX51 with DIC and Phase. The camera is a Canon 6D (full frame). I have not yet published anything in the "My Microscope" section. I will do so shortly. I use Lightroom 5 for post processing, but I am still learning. Also Zeren...

These are all I've got for now. Actually my first images taken with the scope. I will try some more diatoms with the 60X/100X oil to see if I can crank up the resolution a bit. It should be possible to squeeze out some more crispness and resolution than these show. Also, they show quite heavy chroma...

Thanks!

1: Olympus UplanFL 40X DIC, no stacking.
2: Olympus UplanFL 40X Dark Field, 7 images stacked using Zerene.
3: Olympus UplanFL 40x Dark Field, no stacking
4: Olympus UplanFL 40X Dark Field, no stacking

I did not measure the size unfortunately, but I would guess 50-100 microns

I took some pictures last night on diatoms of various kinds. Sharing them here. https://www.dropbox.com/s/adx7g78xnlp8isw/Diatom1.jpg?dl=1 https://www.dropbox.com/s/plkl3myketxcjtt/Diatom2.jpg?dl=1 https://www.dropbox.com/s/ir61t7x2vbmc9f7/Diatom3.jpg?dl=1 https://www.dropbox.com/s/8g96r7w12tly4kn/D...

Thank you Oliver! You may be right. However, I think the "organelles" is moving so fast, I assumed they were something else.

Any idea what kind of creature this is?
Magnification 1000x
Found in human saliva

It is the round organism in the middle that seems to bubble with internal bacterias.

http://youtu.be/ZI7bUYUD2bc