Search found 201 matches

by c-krebs
Tue Jul 12, 2016 9:25 pm
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 8871

Re: America Optical infinity optics

apochronaut, Ultimately I suppose, what matters is the results you actually get. Which to me means if you have the bits and pieces go ahead and try it and see what happens... nothing to lose really. Where it is nice to know how the manufacturers did their optical trickery is when you are considering...
by c-krebs
Tue Jul 12, 2016 8:03 am
Forum: Beginner's corner
Topic: First photos
Replies: 11
Views: 5977

Re: First photos

Yup, a good start. Your white balance for the first two photos looked pretty good. These last two darkfield look as if the color balance was set wrong. (But obviously I didn't see the actual subject). You can usually color balance pretty well after taking the picture when the the image out of the ca...
by c-krebs
Tue Jul 12, 2016 12:29 am
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 8871

Re: America Optical infinity optics

I had been under the impression that current Zeiss and Leica infinity objectives used chromatically corrective tube lenses. But I did not recall where I had seen this. A quick search found some references that seem pretty solid, but none are "from the horses mouth" (Leica). I could never suggest one...
by c-krebs
Mon Jul 11, 2016 11:12 pm
Forum: Pictures and Videos
Topic: Structural color
Replies: 13
Views: 4716

Re: Structural color

Charles, so what are we looking at and with what obj. and lighting? ... Fill us in on the details. The first two are of a jewel beetle carapace, Chrysochroa buqueti. The third is a butterfly wing, Blue-rayed Metalmark (Lyropteryx apollonia). The forth and fifth are wingscales of a Chrysiridia rhiph...
by c-krebs
Mon Jul 11, 2016 8:45 pm
Forum: Pictures and Videos
Topic: Structural color
Replies: 13
Views: 4716

Structural color

One of the subjects that has always fascinated me through the microscope is the observance of "structural" color. Most of the color we see in everyday subjects is due to pigments of some type that absorb and reflect certain wavelengths of light. But there are some dramatic natural (and man-made) col...
by c-krebs
Mon Jul 11, 2016 6:59 pm
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 8871

Re: America Optical infinity optics

Using 45mm Reichert objectives on an AO infinity stand, originally designed for 34mm objectives presents little problem. The 10/20 series has a rather long dovetail guide, for the stage, so it can be dropped 11 mm easily. This might cause a small problem getting certain condensers in and out but I ...
by c-krebs
Mon Jul 11, 2016 7:17 am
Forum: Microscopy accessories
Topic: Carson universal smartphone optics adopter
Replies: 2
Views: 2917

Re: Carson universal smartphone optics adopter

Hopefully it will fit your phone and your eyepiece and thus make your life much easier. Don't use your 25X eyepieces for this, stick with the 10X/
by c-krebs
Mon Jul 11, 2016 6:52 am
Forum: Beginner's corner
Topic: First photos
Replies: 11
Views: 5977

Re: First photos

This is a Desmid, a type of green algae. It belongs to the genus Closterium. There are many species and quite a few with this long thin shape. If you just got your first microscope yesterday, and these are your first two photographs I would certainly say...WELL DONE! - how better photo quality can I...
by c-krebs
Mon Jul 11, 2016 2:59 am
Forum: Pictures and Videos
Topic: Springtails; Collembola.
Replies: 3
Views: 1750

Re: Springtails; Collembola.

These little guys almost look like little cartoon characters. And, as you mention, they are in constant motion. Might be worthwhile to have a small electronic flash ready to go for such occasions. What "hardware" did you use?
by c-krebs
Mon Jul 11, 2016 2:55 am
Forum: Pictures and Videos
Topic: Tardigrade and his Pharynx!
Replies: 19
Views: 5329

Re: Tardigrade and his Pharynx!

These are very good images of a tough subject. They are usually constantly squirming around and they are pretty 3-dimensional so it is normally difficult to get a good composition with adequate depth-of-field. I also noticed what looked like the shell of one...do they shed? Yes they do. And it is pr...
by c-krebs
Mon Jul 11, 2016 2:25 am
Forum: Pictures and Videos
Topic: 2 Vorticella + alga
Replies: 16
Views: 4564

Re: 2 Vorticella + alga

Very nice shot and very nice use of oblique light. I am seeing some good examples of oblique light in the forum. I've often thought it has been one of the most underutilized but yet effective lighting methods. At one point Olympus, Lomo, (and I suspect others) made condensers specifically to make us...
by c-krebs
Mon Jul 11, 2016 2:19 am
Forum: Pictures and Videos
Topic: Pursuit of detail - pollen revisited - stain-attack!
Replies: 21
Views: 5718

Re: Pursuit of detail - pollen revisited - stain-attack!

John,

I'm new here but it sounds like you are having way too much fun at this. (And to think I thought all this subject preparation would be burdensome ;) )
by c-krebs
Mon Jul 11, 2016 2:17 am
Forum: Pictures and Videos
Topic: Fly eye
Replies: 13
Views: 5186

Re: Fly eye

Johann,

Nice job! Looks really good.

It is a useful technique for many opaque objects as long as you have a couple objectives with sufficient working distance to let the light work for you.
by c-krebs
Mon Jul 11, 2016 2:08 am
Forum: Pictures and Videos
Topic: Larger Diatoms Just For Fun
Replies: 25
Views: 7284

Re: Larger Diatoms Just For Fun

Kurt, These are really nice. I'll reserve judgement on the 40X (always best to make a judgement of subject that "fills-the-frame" more). The 100X looks really good. On the second image... which is excellent... you have too much of it's splendor buried in the dark tones. Check out the histogram. A si...
by c-krebs
Mon Jul 11, 2016 1:42 am
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 8871

Re: America Optical infinity optics

Any reason why you don't consider the 3 technically more resolving phase systems, such as Leitz Heine/Pv, Reichert Polyphos/Anoptral and Zeiss equivalents? Those are much more rare and expensive than AOs though. Answered your own question there Fan! ;) I like phase contrast, have used it in the pas...
by c-krebs
Sun Jul 10, 2016 11:39 pm
Forum: Microscopes and optics
Topic: America Optical infinity optics
Replies: 19
Views: 8871

America Optical infinity optics

Years ago I purchased a pretty nice AO 20. I have now perused some of the threads on the forum pertaining to AO (and some specifically talking about the AO 20). It's nice to see since these are really nice scopes and certainly can be a great value. There are some folks here that are very knowledgeab...
by c-krebs
Sat Jul 09, 2016 4:32 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 26
Views: 9556

Re: Live diatoms. Some 100X darkfield.

Any more details? These are with your olympus? I take the dark field is continous light? Are the DIC ones continous light or flash? I assume its an apo dic objective? Stacks? All with the Olympus BHS. First two are 100/1.40 S Plan Apo with a Zeiss Ultra-Condenser (oil) fitted to the stand. (That ob...
by c-krebs
Sat Jul 09, 2016 4:12 am
Forum: Pictures and Videos
Topic: Slicer returns. Initial Pics of Rosemary stalk. Johansen's Quad Stain.
Replies: 22
Views: 5435

Re: Slicer returns. Initial Pics of Rosemary stalk. Johansen's Quad Stain.

b) I haven't done any post processing , yet.... I may well do some post processing etc. and probably some crossed polaroid images etc and post these as some point. Just for fun, I grabbed image #3 (my favorite of this batch), and opened it in Photoshop. A few very quick adjustments and it was reall...
by c-krebs
Sat Jul 09, 2016 3:53 am
Forum: Pictures and Videos
Topic: SRP spiecal canal report IT'S ALIVE!!!!
Replies: 20
Views: 6140

Re: SRP spiecal canal report IT'S ALIVE!!!!

Hello MicroInspector,

The last one remains a mystery to me. :?

The first few are a freshwater amphipod, Gammarus sp. I believe. (As Rodney said...freshwater shrimp)
by c-krebs
Fri Jul 08, 2016 6:36 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 26
Views: 9556

Re: Live diatoms. Some 100X darkfield.

Rodney wrote:Oh, which camera captured those?
Rodney,
I used a Canon 3Ti (just like the one you just purchased ;) )
by c-krebs
Fri Jul 08, 2016 1:02 am
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 26
Views: 9556

Re: Live diatoms. Some 100X darkfield.

Really... Thanks all for the warm welcome and kind comments! You have an enthusiastic group here. I've been a photographer for about forever, but I guess its been about 12 years since I bought that Meiji ML2000 (with it's basic achromats) on Ebay and jumped into microscopy with both feet. Along the ...
by c-krebs
Thu Jul 07, 2016 8:19 pm
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 8735

Re: DSLR Camera

Hi Charles, I would very much like to get your opinion on dedicated microscope cameras, like the Olympus SC50. Do they provide same/better/worse quality images as high end DSLR cameras? Johann, I can't speak directly to your question because while I have used a couple "low-end" ones years ago I hav...
by c-krebs
Thu Jul 07, 2016 7:24 pm
Forum: Pictures and Videos
Topic: Live diatoms. Some 100X darkfield.
Replies: 26
Views: 9556

Live diatoms. Some 100X darkfield.

Here are some live diatoms. (Well... the forth one, Triceratium reticulum, looks to be just the frustule, but it came in with a marine sample and is in a water mount like the others) The first two are darkfield with a 100X objective. With my set-up that makes the on-sensor magnification 210X. Of all...
by c-krebs
Thu Jul 07, 2016 1:19 am
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 8735

Re: DSLR Camera

Charles, What do you think about the new Panasonic GH4, which has EFSC and 4K video? Are there any other micro 4/3 that you would recommend? For afocal - camera lens - scope eyepiece coupling, which F stop number do you recommend for camera lens? I haven't really studied most of the 4/3 cameras in ...
by c-krebs
Wed Jul 06, 2016 10:00 pm
Forum: Camera systems and imaging
Topic: DSLR Camera
Replies: 19
Views: 8735

Re: DSLR Camera

Rodney, Can I ask what microscope "coupling" you intend to use? What microscope? The reason I ask is that there are a couple very attractive MFT ("micro four thirds") available. These afe mirrorless but can have some real advantages on a scope. There are some characteristics about the microscope set...
by c-krebs
Wed Jul 06, 2016 8:19 pm
Forum: Pictures and Videos
Topic: Spring water.
Replies: 14
Views: 4796

Re: Spring water.

Nice shots! See these fairly regularly in old samples. Definitely "left-overs" from decayed plant matter. See the second image (a photomicrograph) on this page http://waynesword.palomar.edu/trjune99.htm for a pretty good look at them in situ. I'll leave it to the plant experts like John to tell us t...
by c-krebs
Wed Jul 06, 2016 7:55 pm
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 15436

Re: Exorcising a Vibration Demon

Kurt, Personally I'm leery about the T6i (and T6s) after some reports and images about a potential problem with microscopy and astro-photography. See this thread. "pwnell" is a good photomicrographer and very particular, so I thought perhaps he was looking "too hard", and maybe altering contrast way...
by c-krebs
Wed Jul 06, 2016 7:14 am
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms
Replies: 19
Views: 7239

Re: Tube-dwelling diatoms

You mention your setup is modified for electronic flash. Do you mind sharing how this is done? I can only imagine the improvement a flash would make when imaging fast moving specimens. I've used about 4 different methods over the years. The one I use now is for my Olympus BHS. This stand has a quic...
by c-krebs
Wed Jul 06, 2016 6:14 am
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 15436

Re: Exorcising a Vibration Demon

Kurt, The T3i, as with the other digital Rebels, always uses EFSC when a continuous light exposure is made from live-view. There is no setting to turn it on or off. (Different story when using a Canon electronic flash). With the 50D and other"xxD" series Canons, as well as the FF models you need to ...
by c-krebs
Tue Jul 05, 2016 8:37 pm
Forum: Camera systems and imaging
Topic: Exorcising a Vibration Demon
Replies: 36
Views: 15436

Re: Exorcising a Vibration Demon

I need to replace a Canon, and am watching the new 80D's prices creep downwards. I wonder if anyone has tested the shutter Was reading through this thread since "vibration" has been a constant nemesis in microscope photography. I can offer some info to "Interference" in regards to his question abou...