MicrobeHunter.com Microscopy Forum

woyjwjl wrote: I found this prism. Is it the one you use?

Yep, those are the ones I have.

Thank you for your detailed reply. I noticed that your DIY DIC prism is still missing 20x. Is there any problem? Sincere wowjl I am getting really good DIC at 20x now with one of my DIY prisms and my Olympus short-barrel planapo 20x 0.65. Dark and even background. No images to show, unfortunately. ...

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Yeah, there's definitely enough tolerance in DIC to be worth trying mismatched systems. It's also worth remembering that even the original BH-2 system was only designed for Splan Achromats, so people (like Charles Krebs) using it with Splan Apo's are actually using a mismatched system anyway, which ...

No. It's for the Vanox AH. It won't fit the condenser holder on the BH2, and, since it doesn't have the LB sticker, it will most likely have three prisms designed for short-barrel objectives and AH intermediate tube.

Here's a LB version for the Vanox AH: https://www.ebay.com/itm/224661096841 Actually, I just realized that it says that it only has 10,40 and 100x prisms, just like the original short-barrel version. Now that I think of it, I think that you've shared that info with me before, PeteM. Then I guess tha...

https://www.microbehunter.com/microscopy-forum/viewtopic.php?p=73842#p73842 Here are photos showing the difference between the AH (short-barrel) and BH-2 (long-barrel) intermediate tubes. The intermediate tube in the photo you linked to is of the BH-2 long-barrel type, but, as PeteM points out, the ...

Great Post, Pete. I was really tempted by one of these a couple of years ago. Came with a full set of PlanApo's, for $999. Of course, shipping it across the Atlantic made it too much of a hassle. Being the flagship model, I'm thinking that a greater proportion of these were sold with high-end optics...

That's quite an attractive set of features. The Zeiss Plan Neofluar 63x 1.2 W Korr is pretty much the only one thing that comes to mind, but it doesn't have an iris: https://gimitec.com/epn/wp-content/uploads/2021/01/zeiss-microscope-plan-neofluar-63x-1-2-w-korr-160-0-12-0-22-water-objective.jpg?v=1...

That's a nice one from the original CF series. Nikon never updated it (CFN) before going infinity.

Not for sale, is it?

Totally crazy that no-one has bought this from you!

Impressive start ! I like the DIY cube ; I assume that some artefacts in the photos originate from the illumination train upstream of the cube. Because the choice of filters is OK (for a fluorophore excited by UV). Especially the plant images appear to be non-uniform illumination as you hinted. Ind...

nematos wrote: ↑

Sat Mar 19, 2022 4:29 pm

Really incredibly beatifull picture!

Thank you! I was thrilled to see how well they turned out.

The microscope setup: https://i.imgur.com/K3UtOas.jpg And the 3D printed filter cube: https://i.imgur.com/26Zj8at.jpg The cube is just a prototype, temporarliy assembled with duct tape. I will need to fix a few small design mistakes and print a new one. When I am happy with the design, I would be ha...

A couple of months ago, I was sent a LP-DAPI filter cube from Optolong. In return I promised to share the results online. I thought it was a good excuse to force me to finish my DIY fluoresence build, and actually try to take some pretty pictures. It turned out to be a little tricky to fit the filte...

It depends a little on whether you want to use as much of the LED output as possible for excitation, or if you want to filter out the visible light and focus on wavelengths shorter than 400nm. If you do autofluorescence, shorter wavelengths are definitely better, and a 365nm LED would be much better...

They also are required to pick the image up 18mm down the tube, which is a focal distance unique to leitz. I don't understand :( https://www.mikroskopie-forum.de/pictures002/87737_5297393.jpg This image, which is found in one of the links in the thread I linked to above illustrates the issue. Leitz...

I've always been a bit confused by the many different varieties of Leitz eyepieces. But based on this discussion: https://www.microbehunter.com/microscopy-forum/viewtopic.php?t=13733 I believe that it is a general rule that eyepieces made for 170mm Leitz microsopes does not have a field number writt...

Good list, but you're missing the 12x and 63x objectives!

Robert is pretty generous with sharing details about his photos. On the page below, you'll see that he used DIC illumination for that photo: www.canadiannaturephotographer.com/ciliates.html Buying a DIC capable microscope is not cheap, usually a couple thousand USD. A fairly similar color scheme can...

I've built a foot switch to trigger my camera. This is especially nice when shooting pond critters as it allows you to focus with one hand and move the stage with the other.

Here's instructions:
www.instructables.com/Canon-EOS-Foot-Pe ... ter-Remote

While I agree that mycology is one area where you really need a high NA oil objective, I don't think it's extremely demanding per se. Phase is convenient, but if you are okay with some basic staining, then pretty much any microscope with a 100x 1.25 oil objective and an abbe condenser should work we...

This is what I've done with my AH-NC NIC/phase contrast condenser for my Vanox AH. https://i.imgur.com/AmVg4LG.jpg I've replaced the phase rings with some Nikon Optiphot epi-DIC prisms. Through trial and error, it has allowed me to expand the range of objectives I can get good DIC with somewhat. As ...

That place seems like an elegant place to put filters if you are a producer that wants to make nice, ready made filter inserts. But are you sure that it is really the only place you can place those filters? The requirement is that a darkfield stop should be placed at a plane that is conjugate with t...

£800 in 1969? that is equivalent to £11000 today.

...according to:
https://www.inflationtool.com/british-p ... amount=800

Always fun, rarely useful!

Wonderful appearing scope - and the first Apophot I've seen. Thanks for posting it. It looks as if there is room to move the stage down and use 45mm parfocal objectives as well? Do the nosepiece and magnification changer use the same circular dovetails as later Nikon nosepieces? If so, it might be ...

dtsh wrote: ↑

Fri Jan 28, 2022 3:39 pm

That looks like a very nice beast of an instrument!
What is the large, black, circular part on the lower right of your last image?

Magnification changer maybe?

What condenser is it? Looks like an achromat aplanat (nice!), but does this microscope come with any special illumination techniques?

Magnificent microscope! Definitely a close cousin - or perhaps nemesis! - of my Olympus Vanox AH. The short-barrel apochromats from this era can be quite affordable and are definitely worth chasing down. My Vanox also came with simple plan achromats that I didn't like, but I have since tracked down ...