MicrobeHunter.com Microscopy Forum

How would you center the optovar on this stand?

BramHuntingNematodes wrote: ↑

Tue Jun 16, 2020 7:26 pm

looks like the wheel of a pirate ship!

That is a Zeiss Linnik-type interference microscope

Great work!

Out of curiosity, when you take the photo do you have a green filter in the light path and shoot in B&W?

Many thanks to everyone for the interest. While I find these really cool from an aesthetic perspective, I’m struggling a bit to see the practical application of this technique. What would this method reveal that others do not? It allows you to measure the phase difference between an object of intere...

After searching for a while I finally found a complete Jamin-Lebedeff interference set that I could install on my Phomi. Its a system of condensers and objectives where each pair contains an optically identical crystal used for beam splitting and recombining. A reference beam is compared to a sample...

Thank you 75'

I thought its trying the get rid of the little guy, to me it looked like its pushing it away.

For a med student you could get a nice binocular Zeiss Standard with a 2,5 to 100x achromat (or maybe even plan) objectives for about $300. Its all you really need for histological sections (I think thats what they do in med school). Depending on which part of the world you are it could be that some...

You can observe the kinetosomes moving back and forth on the axonemes.

Taken with Planapo 63/1,4 with DIC. Depending on your internet connection you can choose 1080p for best video quality.

What about this one?






https://www.ebay.de/itm/MIKROSKOP-KOMPE ... SwimRe3d1u

Video unavailable
This video is private.

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...

Can you estimate how the results of your measurements of cover slips were spread? Natural gaussian spread or a gap in the middle? For me it is the question when I buy loose tolerance cover slips e.g. #1.5 (0,16mm to 0,19mm): Are the 0,17mm ones included or do they sell them separately? @Wes: Here i...

I use FFmpeg. Its free and many of the video editors you know are based on it. You can do pretty much anything with it, its really good but has a bit of a steep learning curve.

What about this one? https://www.ebay.co.uk/itm/Bugelmessschraube-0-25-mm-mit-1-Nonius-0-001-DIN-863-Mikrometer/263342487388?hash=item3d506f775c:g:bCcAAOSwenJaGCJe That looks nice, I would like to see the brand though. Good point, not clear what the brand is (may explain the rather accessible price...

MicroBob wrote: ↑

Fri Jun 05, 2020 8:52 pm

Your aim (precise and round) makes sense, especially for work intensive arranged slides.

Other than ringing the cured slide on a ringing table is there any other particular reason to use round coverslips? I somehow doubt square coverslips would be much worse, but maybe I miss something here.

Thank you 75' and Bob

What about this one? https://www.ebay.co.uk/itm/Bugelmesssch ... SwenJaGCJe

Does it seem reasonable?

Hm if I had a micrometer I could just bin them and select the 0.17 bin for cleaning and further processing.

[/quote] Beautiful work, Wes! Thanks for this display of good microtech. charlie guevara BTW, I always wonded if I could collect and save the occasional huge pennate naturally cleared frustules I encounter in wetmounts from time to time...to one day try and mount these frustules. Thanks again Wes fo...

Does that adhesive layer offer a certain initial tack to be able to get the diatoms off the needle? Hi Bob! I don't think this particular adhesive offers that although I have not extensively compared it against a plain surface coverslip or other adhesives. In fact the adhesive layer itself is very ...

Many thanks Bob! I have taken your advice on board and finally I stopped having crushed diatoms in the mount.

Thank you all for encouraging remarks! Now to answer some of your questions. I boil the samples in 30% hydrogen peroxide with some hydrochloric acid followed by several washes with distilled water and finally the diatoms are resuspended in ethanol to prevent mold growth. I make a strew slide from th...

For a brief while now I've been entertaining the idea of making my own diatom arrangements. After reading different accounts on how its done and a bit of struggling I present you my very first attempts. Below are a few arranged diatoms that I collected from the nearby river, which I cleaned and then...

Incredible images! I especially like the second one showing these rather fine details. Thanks for sharing.

The diatoms shop in Holland sells pre-cleaned diatom samples (Website in English) and Mountants and other gear you may need. So no unknown dangerous acids to mess around with: https://www.diatoms.nl/index.php?seo_path=samples Happy customer only, Best, deBult. I haven't personally had a go with the...

Amazing shots. 3rd one is my favourite.

What mesh size plankton net did you use?

Charles, I do not have financial relations with suppliers of Falcon tubes, and I do not remember their price, but for what its worth, they never leak. Neither the 15mls, nor the 50mls. And, although they are dispensable, I reuse them many many times. For my last field mission, the 50mls where great...

Sir wrote: ↑

Wed May 27, 2020 7:57 am

Some soap bubbles 40x - 100x

Nice, good quality!

Hobbyst46 wrote: ↑

Fri May 29, 2020 1:44 pm

diatoms.nl carry Euparal.
...
Although I did not know that Euparal is useful for mounting diatoms.

OP didn't mention diatoms tho

Either way I just bought Pleurax from Michel (the owner at Diatoms.nl) and its good quality plus he seems like a really nice guy.

Nice microscope!!

Looks like you're quite lucky because you have a complete DIC system on an otherwise great Zeiss scope. After you go over Charles' documentation on the DIC system please let us know how you like it and post some DIC imagery if you can. That would be exciting!