MicrobeHunter.com Microscopy Forum

Awesome video! I always wonder, how such slow and squishy beings can catch any food at all. Thanks. This particular species seems ok capturing all sorts of highly mobile organisms, things like Chilomonas , Chilodonela and even whole rotifers. They are so massive that they probably appear as normal ...

Hi Wes, I have seen this many times myself. I think this is an early stage in the growth of zoospore of Oedogonium. The tentacle structure is the holdfast that the alga uses to attach to the substrate. With floating coverglasses, I have seen nice images of the holdfast attached to the slide, as sho...

Thanks everyone for the comments. I have now attempted to hunt the amoebas one by one and try to establish amoeba cultures. Lets how it goes..

Finally I found a huge amoeba that was visible with the naked eye. Must have randomly picked it up while mounting a patch of algae with worms (which turned out far less exciting than the amoeba). I think this is Amoeba proteus but feel free to correct me if you suspect otherwise. aPI2oC2x8qA Video i...

Very nice pictures and write-up. I almost invariably find these two as residents of floating coverlips. Being stuck to the underside of the coverslip makes them particularly photogenic.

Hi Dr. Mary, If you can elaborate a bit further on your goal and expectations we might be able to provide more specific advice. A pathologist would be able to make great use of cheap (under 1000 USD) microscope, provided a histology technician supplies him/her with nicely stained tissue sections (co...

Expansion wrote: ↑

Fri Apr 01, 2022 3:38 am

What camera do you use?

I use an old Canon 50D

Is it 630x? Hi Phill, its a crop from an image taken with a 40x objective and a 10x eyepiece. Hi Wes, I have seen this many times myself. I think this is an early stage in the growth of zoospore of Oedogonium. The tentacle structure is the holdfast that the alga uses to attach to the substrate. Wit...

Thanks everyone for the comments, I found a bunch of these and I've never seen them before.

Expansion wrote: ↑

Thu Mar 31, 2022 2:58 am

Interesting, are those appendages not pseudopodia?

They are appendages, I think they are some sort of rhizoids or have a function similar to hyphae in fungi like growth tips.

I found this organism in a somewhat putrid little pond. At first I thought it was some sort of Difflugia but upon closer examination I believe this is not correct. The appendages ("tentacles") that you see on the lower left side are immobile. Is it some sort of an algal reproductive structure? https...

Hi Wes and Hobbyst46, thanks for the information. Might have to look for one of these. Wes, I did post an inquiry on the German microscopy forum and was basically informed that these plan Neofluars work only with the sliders for each objective, as in your newer Zeiss equipment. Could you provide th...

I am wondering if one of these plan neofluars would work with the original Zeiss system. Would you have any insight into this? I asked someone who has the old DIC system and the 40/0.9 plan-neofluar and they told me it doesn't work particularly well. On the other hand the 25/0.8 plan-neo apparently...

Really nice results and I love your 3d printed adaptation for the filters. If you can, try out some fluorescent dyes like acridine orange and DAPI.

This is impressive photography, let alone for an 11 year old one.

So your link and diagrams indicate that the effect of a lack of an arrow straight ray distribution with an aqueous sample , using a glass coverslip is minimal?....not worth pursueing homogeneity? From what I understand the difference in refractive index between oil+coverslip (n=1.55) and water (n=1...

presume the principal is similar to a correction collar. Exactly, not only you can select the immersion medium but you also have the option to select whether the sample has a coverslip or not. So you do therefore notice a difference in the image quality, depending on the r of the sample and the sel...

I really like the multi immersion PLAN-Neofluars from Zeiss. Mainly because water immersion removes spherical aberration in water embedded sample, much more so than oil immersion. And I mostly look at protists so this is ideal. https://scontent-sof1-2.xx.fbcdn.net/v/t39.30808-6/262090382_43461267493...

Nice shot. Tell us more about your setup.

Welcome, Davide. Looking forward to seeing photomicrographs from you.

Nice! Is the brown organelle a chloroplast?

Kurt, you have inspired me to get a plankton net. Great catch and write-up.

Great shot. I have always been fascinated with the extrusomes and their arrangement.

Beautiful image, thank you Wes. Thank you for the forceps technique tip. My sense of this still image, is that the amoeba is moving to topleft...and is actually going to leave these frustules behind in short order. Beautiful image. thanks, charlie g Thanks Charlie. The amoeba had long axopodia but ...

Thanks everyone for the interest and comments! How do you fish the coverslips from the surface of the water? I have tried using a coverslip forceps but I seem to end up with one corner or edge getting immersed. Hi Tim, What works well in my hands are fine point tweezers with a bent tip (I do the ben...

Interesting find!

Found this in the local river after floating a coverslip on a water sample for a few days.

Nice find, in my experience spring is the best time to find Paramecium bursaria.

I assume you want to use it for fluorescence microscopy and by preparing I would assume you ask how to make solution and use it instead of synthesizing it yourself. If you have access to DMSO measure out a given amount and prepare a 10mg/ml solution based on the pre-measured mass. Otherwise you can ...

Very nice catch. And your photos are amazing!

Thanks for sharing your plankton catch with us Kurt. These images are superb