MicrobeHunter.com Microscopy Forum

Hi everybody, cross section of pathos leaf stem (or petiole). https://i.ibb.co/X75WCrv/Botanic01.jpg 4X Achro Na 0.10- Condenser Aperture 0.10 - 10 Images stacked in PS Safranin and Methylene Blue staining cross section of a succulent stem https://i.ibb.co/g6Hbw7L/IMG-7322.jpg 4x Plan Achro NA 0.10 ...

Thanks KD.

The footage is certainly top notch. Never seen anything like that. Is there any chance that I can find some flagellated bacteria too? I really want to observe it myself. They seem to abound in oxygen depleted waters but are quite common in general. Many pathogenic bacteria sport flagella but in som...

The footage is certainly top notch. Never seen anything like that.

Is there any chance that I can find some flagellated bacteria too? I really want to observe it myself.

Welcome Element 56.

Being skilled in machining will be a HUGE help when it comes to microscopes. Making filter holders, adapters and custom parts would be much easier.

Any particular interest toward Barium?

Is that a serial RS232 port on the adapter? What does it do?

Very nice and inspiring shots Hobbyst46.

75RR you will have to trust me. I did try and put them in the main body of the forum post, but there was too much info there. Plus I really don't have the time or expertise for anything sinister. It's a long list of microscope lenses and their properties. But is the best way to get you this informa...

Saul
It's truly an outstanding shot. It shows the amount of work and effort you put into it.

Hi Daroush, interesting subject and good contrast technique! The COL looks like DIC here. COL is a bit fiddly to setup in my experience. Perhaps you could reduce the vignetting by image editing. Bob Thanks Bob, I just got bored (being more than 25 days at home) and tried my eyebrow hair under the m...

Its for embedding tissues into paraffin blocks and enables very thin sections to be cut with a microtome. Check out this link for much more detail procedures https://www.open.edu/openlearn/science-maths-technology/biology/introduction-histology/content-section-2.2 This video is also very useful: nUj...

Nothing spectacular, but hey, it was new to me:


40x Plan Achro 0.65 / Condenser aperture fully open / COL ring size: 19mm / Cross polarization + Cellophane as compensation plat

Any ideas to make it better?

One thing to watch out for, is the stability of the system. DSLRs are heavy and there is no real need for the mirror on a microscope. It's kind of a design holdover from the SLR. Buying new; there are few reasons not to investigate a mirrorless camera. On second though I was going to come back and ...

I guess is't a field extender for low magnification like 1x or 2X objectives.

Hi,

With 1000 USD budget, I would recommend a DSLR camera. You'll have full control over exposure settings and much higher dynamic range. Something like Canon EOS 750D/800D.

Hi Daruosh and thanks for this information :D I am curious what the price could be on trinocular head etc but i think i want to wait and buy some better microscope in the future. I want a better condenser and diaphragm also and better objectiv, but i will load up some photo on my head to you. I am ...

AntonioGPS wrote: ↑

Wed Apr 08, 2020 10:02 am

I can see them within the message. Maybe is a problem with Google permissions.
I will ensure that they can be shared

Indeed, this could be the problem. I ensured a open access. Please, let me know whether they can be seen now

Still not visible.

Hi AntonioGPS,

I guess there's a problem with your pictures, I cannot see them, tried on 2 different computers.

Daruosh where can i find information on the webb for trinocular etc. for Novel N-200M? Novel makes 3 types of (160mm tube length) trinocular heads. If you post a picture of the head dovetail, we can determine the correct part. I ordered mine for Novel N-180M from China (through a 3rd party dealer)....

Thank you very much Bob for all information :D I have a Novel N-200M now and i am rather pleased with that microscope. I think it is very sharp but i want a microscope with trinocular head and phase contrast. I love this hobby and i want to learn more and more...And i love to film microorganisms :D...

Wow. It's really stunning. Well done.

They are better for the diatomist than electric centrifuges for the tiny Eppendorf tubes because the glass tubes hold 15ml of fluid. For some diatom cleaning methods it is necessary to remove a chemical quickly and there is no time to just let the diatoms settle by themselves. Hi Bob, My bench top ...

Here's mine next to a 600ml beaker

As an aside - anyone have a hand centrifuge and have they found it practical/useful? I have this model and I found it to be OK, not the best, but it does the job: https://images-na.ssl-images-amazon.com/images/I/71N4KP1YKdL._SL1500_.jpg https://www.amazon.com/Eisco-Crank-Centrifuge-Capacity-Tubes/d...

Wow, once you understand the actual shape of this particular diatom, you realize the beauty of their image.

Thanks for sharing your pictures and more details.

Fascinating images Bob. Never seen diatom images like that. How do they look like under bright-field?

I'm no expert, but I guess it's "limnias melicerta".

Very impressive. Perfect choice of background music as well.

I cannot help you ID those wiggling microorganisms, but the footage is beautiful to watch

I'm thinking about a similar project and cobbled a 10X el cheapo objective with an LED and an eyepiece. It turned out the mechanical fit and finish is important and my 3D prints modeling (at least in my case) is not precise enough, everything needs to be on a very same axis and same horizontal level...