MicrobeHunter.com Microscopy Forum

Couple stacks of GABA, Urea & soy sauce, using this illumination setup
Sony A6300, Microphot, Nikon BD 20x infinity and LED/flash illuminator

Some notes about this setup:
https://www.photomacrography.net/forum/ ... 25&t=42793

Sony A6300, Microphot, infinity Nikon 20x BD, 135mm TL, 1.4x teleconverter, 198photos, Pmax/Dmap

Bigger : https://live.staticflickr.com/65535/506 ... 2bee_o.jpg
Video: https://www.flickr.com/photos/bauras/50 ... ateposted/

Thanks :) I'm looking for at least "some" physical relief, the so called poor's man DIC. I'm starting on microscope imaging and the Koehler brightfield yields quite "flat" imaging. I was filming tardigrades over the weekend, and I was pretty happy, but I saw footage obtained with the same microscop...

...My guess is that you are looking for maximum physical relief? Such an effect requires a high degree of precision. It doesn't necessarily happen at Koehler and only a smàll + or- in the location or orientation of the components can make the difference between hey, that's o.k. and wow, that's grea...

OK, mystery solved: https://www.facebook.com/photo?fbid=4625001210905631&set=pcb.1628375254010550 https://www.facebook.com/SciencePhotoLibrary/photos/a.399498442948/10158410026142949/ "The appendages on top of the head are air sacs used to control buoyancy. The larvae of these insects are aquatic an...

Bemoc wrote: ↑

Tue Oct 13, 2020 6:49 pm

Saul that is beautiful and even suspenseful. I dig the music too.

Thanks ! Fully agree - music is great

Traleah wrote: ↑

Sun Oct 04, 2020 6:28 pm

Saul,
Are you still working on this project? Any updates?

Got micro leds , but did not have time to play with them. Do not expect too much from them.
Played with some masking around edges, using aluminum foil

DonSchaeffer wrote: ↑

Sun Oct 04, 2020 10:11 pm

I love this solution. I wish I had your skills. I just use a mounted flashlight to do a rough approximation to this.

Thanks Don !
Some skills and lots of the stupid patience

Fruit fly larvae
https://youtu.be/_uODOPAIzBc

DF/Pol/Obl illumination, Nikon Microphot , Sony a6300, 10x objective, 125mm TL

iconoclastica wrote: ↑

Thu Aug 27, 2020 2:38 pm

It is real, not the perspective. Mine is between 7 and 7.5mm deep

Thanks Wim, useful information !

Gaba + Urea, pol/DF/oblique, 20x, Sony A6300/Microphot

iconoclastica wrote: ↑

Wed Aug 26, 2020 8:14 pm

Mine:

Hmm, depth looks different (or just perspective ? ), mine is 14mm ....

iconoclastica wrote: ↑

Tue Aug 25, 2020 8:38 pm

...I gather there are two different models of the achr 0.90 about, for my drawing was exact too (at least on the outside)...

Design is based on this model:

... I have always read these modifiers ought to sit near the diapragm, but in this contraption they are much lower. Isn't the position perhaps that important? ... On my 0.90 achromat the diaphragm resides c. 30mm above the bottom plate. Nikon Achr 0.90 is not the best candidate to use with DF inser...

DonSchaeffer wrote: ↑

Sat Aug 22, 2020 3:27 pm

Very interesting. The stereoscopic perspective really works. How did you do this?

https://zerenesystems.com/cms/stacker/d ... eticstereo

Nikon Microphot, Nikon BD 20x infinity , Sony A6300, using DF/Obl/Pol condenser insert , variable sliding oblique filter , LED/flash illuminator , 196 photos, Zerene DMap, Stereo Photo Maker. Stereo 1-web320x1024U100-G15.jpg Bigger: https://live.staticflickr.com/65535/50253945057_1a9cc7afce_o.jpg

KurtM wrote: ↑

Thu Aug 06, 2020 11:15 pm

Good heavens, I'm wincing just looking at that thing! But I have to agree with PeteM: "Bonus points for making the most of the experience. Awesome image."

Thanks Kurt !

Last photo from this series, another side, quick stereo:

Bigger

Thank you all for your kind words and comments !

Stereo version:
Bigger

Kidney stone, 2x stack&stitch panorama (138 photos each), 10x, Zerene and PS
Bigger

Thank you ALL for your kind comments !!!

Still I'm not getting any notifications if somebody replied to my post, sent message etc. Checked/rechecked/resaved all boxes in the user's panel, did not help ... Thanks, Saul I've noticed that notifications only happen when someone hits the quote button on one of my messages. It does not seem to ...

Yes, resubmitted few times.

Still I'm not getting any notifications if somebody replied to my post, sent message etc.
Checked/rechecked/resaved all boxes in the user's panel, did not help ...
Thanks,
Saul

Beta version is coming - light from both sides (separate dimmers) & sample can be rotated.

3D model (alpha version):
https://www.thingiverse.com/thing:4543961

I liked the result, next step is to place more LEDs around the perimeter, in order to have more light control. Anybody tried this type of illumination already ? To my shame, Saul, I still have not summoned-up the courage try mounting some tiny ‘surface-mount’ LEDs that I bought last year. Ordered 0...

micro wrote: ↑

Thu Jul 16, 2020 3:12 am

How does it do with smaller subjects like rotifers?

Did not try yet