Search found 4290 matches
- Mon Jun 26, 2023 1:11 pm
- Forum: Pictures and Videos
- Topic: Recrystallization
- Replies: 1
- Views: 372
Re: Recrystallization
Cool stuff! Which chemical is it ? Does it yield the same crystals from water and acetone ? forgive me the stupid question, but could it be that the crystals were just washed by the acetone, not dissolved, and when the acetone evaporated, they just reappeared on the slide, only shifted to other loca...
- Sun Jun 25, 2023 3:37 pm
- Forum: Microscopes and optics
- Topic: Adding Darkfield to Old Zeiss Standard; Possible?
- Replies: 4
- Views: 1091
Re: Adding Darkfield to Old Zeiss Standard; Possible?
Turret phase contrast condenser Cat . 465277 includes a DF position that gives nice DF with low-mag dry objectives. The condenser must be oiled to the slide bottom for that purpose. So, effectively, I'd have to oil that turret condenser to the bottom of my slide for all DF , if I'm reading that cor...
- Sun Jun 25, 2023 3:33 pm
- Forum: Pictures and Videos
- Topic: High NA Diatom Images
- Replies: 13
- Views: 1721
Re: High NA Diatom Images
Thanks, Michael. Thanks, josmann.
I have a DF oil condenser and a 100X1.3 objective and a green IF and polarizer and analyzer. Problem is, focusing
becomes difficult because the combination of all that in series darkens the FOV so much. Worth a second try though.
I have a DF oil condenser and a 100X1.3 objective and a green IF and polarizer and analyzer. Problem is, focusing
becomes difficult because the combination of all that in series darkens the FOV so much. Worth a second try though.
- Sun Jun 25, 2023 6:58 am
- Forum: Pictures and Videos
- Topic: High NA Diatom Images
- Replies: 13
- Views: 1721
Re: High NA Diatom Images
Impressive !
I tried to see details of your microscopy setup but did not find them in the link.
I tried to see details of your microscopy setup but did not find them in the link.
- Sat Jun 24, 2023 7:55 am
- Forum: Microscopes and optics
- Topic: Adding Darkfield to Old Zeiss Standard; Possible?
- Replies: 4
- Views: 1091
Re: Adding Darkfield to Old Zeiss Standard; Possible?
Turret phase contrast condenser Cat . 465277 includes a DF position that gives nice DF with low-mag dry objectives. The condenser must be oiled to the slide bottom for that purpose. The Ph3 port of the various turret phase contrast condensers gives acceptable DF with the 6.3X and 10X objectives. May...
- Thu Jun 22, 2023 12:26 pm
- Forum: My microscope
- Topic: Connected a 110V microscope to 230V
- Replies: 6
- Views: 2676
Re: Connected a 110V microscope to 230V
Optiphot halogen lamp is indeed 12V 50W - re-verified with the Manual
Optiphot 2 halogen lamp is indeed 12V 100W - re-verified with the Manual
Optiphot 2 halogen lamp is indeed 12V 100W - re-verified with the Manual
- Tue Jun 13, 2023 1:12 pm
- Forum: Microscopes and optics
- Topic: Comparison of optomechanical design of immersion objective tips of Zeiss, Olympus, Nikon
- Replies: 13
- Views: 2523
Re: Comparison of optomechanical design of immersion objective tips of Zeiss, Olympus, Nikon
Important information to inverted microscope users. Those are probably features that the manufacturer/seller never mentions... Dripping oil is really nuisance in those cases. I have even heard of pumps or other devices for catching the overflow.
- Tue Jun 13, 2023 1:01 pm
- Forum: Introduce yourself
- Topic: Greetings from Lake Erie, USA
- Replies: 9
- Views: 2126
- Tue Jun 13, 2023 7:41 am
- Forum: Introduce yourself
- Topic: Greetings from Lake Erie, USA
- Replies: 9
- Views: 2126
Re: Greetings from Lake Erie, USA
Welcome. On the Zeiss Microscope, it is good that the X-Y control knob on the stage is placed along the left side of the microscope. This way the condenser rack knob is easily accessed. Would you tell the details of the Zeiss Microscope camera optical train ? photo eyepiece ? additional lenses ? whi...
- Mon Jun 12, 2023 10:05 pm
- Forum: Microscopes and optics
- Topic: Need help with a stuck part on a Zeiss PM3 tube head
- Replies: 26
- Views: 10244
Re: Need help with a stuck part on a Zeiss PM3 tube head
Did you try a drop of light machine oil on the stuck pin ? penetrating oil maybe ? that part is over-engineered IMHO.
- Mon Jun 12, 2023 10:03 pm
- Forum: Microscopes and optics
- Topic: Leitz Orthoplan darkfield: dedicated DF or phase condenser?
- Replies: 8
- Views: 3972
Re: Leitz Orthoplan darkfield: dedicated DF or phase condenser?
Ok, thanks guys for the informative comments. What about using the dedicated DF condenser that is intended for larger aperture objectives and oil immersion with those dry high aperture objectives? I would like a DF system without using oil immersion. However, I do happen to have a set of Leitz wate...
- Sat Jun 10, 2023 1:31 pm
- Forum: Illumination Techniques
- Topic: High NA darkfield comparison
- Replies: 16
- Views: 8709
Re: High NA darkfield comparison
Here is the Baker Ultra Condenser: Baker ultracondenser.jpg food for thought? 1. In the Baker condenser, Is that a specimen flow-chamber instead of a slide ? the specimen flows by means of gravity ? or is it a flow of immersion oil :? 2. Brownian motion is a fascinating demonstration of light scatt...
- Wed Jun 07, 2023 1:06 pm
- Forum: Illumination Techniques
- Topic: High NA darkfield comparison
- Replies: 16
- Views: 8709
Re: High NA darkfield comparison
I wonder if NOT using a conventional slide but a coverslip might push the NA of the condenser past 1.4. Again one would have to do the math to see what that effect would be. Wild guess -did it work, someone would have certainly suggest it a long time ago ! Moreover, someone would have designed a DF...
- Tue Jun 06, 2023 1:49 pm
- Forum: Illumination Techniques
- Topic: High NA darkfield comparison
- Replies: 16
- Views: 8709
Re: High NA darkfield comparison
It is possible that Zeiss coined the term Ultracondenser because in the early years of DF , the technique was referred to as " Ultramicroscopy" because using DF particles or inclusions could be detected that were below the theoretical limit using BF. I think so too, since when I first read about ul...
- Tue Jun 06, 2023 9:00 am
- Forum: Illumination Techniques
- Topic: High NA darkfield comparison
- Replies: 16
- Views: 8709
Re: High NA darkfield comparison
Hello all, Here is another small test of the Ultracondenser (cardiodid dark field oil condenser), pushing towards the high NA limit. I do not have any objectives of NA>1 with iris or funnel stops. Intriguing post.... I'm not familiar with the "Ultracondenser"; is that a Zeiss product and what's its...
- Sun Jun 04, 2023 8:11 pm
- Forum: Microscopes and optics
- Topic: Ideas for objective turret digital feedback?
- Replies: 10
- Views: 2328
Re: Ideas for objective turret digital feedback?
I think that on the BX, one manually registers an objective into the software. The software recognizes the objective that sits at the optical train, and the ordinal number of the turret position. One installs the objective and tells the software which one it is, in the operating software of the moto...
- Thu Jun 01, 2023 7:45 am
- Forum: Morphology and Behavior
- Topic: Life in a High Desert Rock Pool? Surprise!!
- Replies: 19
- Views: 7394
Re: Life in a High Desert Rock Pool? Surprise!!
Granite itself, when eroded and disintegrated by the elements, forms beautiful and rich sand, where the three main components - quartz, feldspar and mica - are separated. Fun looking with and without polarized light.
- Wed May 31, 2023 7:06 pm
- Forum: Morphology and Behavior
- Topic: Life in a High Desert Rock Pool? Surprise!!
- Replies: 19
- Views: 7394
Re: Life in a High Desert Rock Pool? Surprise!!
...2. No fluorescence dyes were used and I do not know what that red stuff is though I've seen it elsewhere in the internets. Interestingly, I can see these rotifers in my water sample and they have a rust-red color. When I take it out of the fridge, there's a rust colored sludge at the bottom that...
- Mon May 29, 2023 8:19 am
- Forum: Morphology and Behavior
- Topic: Life in a High Desert Rock Pool? Surprise!!
- Replies: 19
- Views: 7394
Re: Life in a High Desert Rock Pool? Surprise!!
A beautiful presentation ! of the scenery and sample. Questions: 1. Details of microscope, camera etc ? 2. The inside of most or all of the rotifers includes a bright red corpuscle. Was it fluorescence (combined with DF) ? the leaky crushed rotifer was devoid of that red stain. 3. Were the green cir...
- Sun May 28, 2023 10:09 am
- Forum: Illumination Techniques
- Topic: 4500K or 6000K LED?
- Replies: 6
- Views: 2278
Re: 4500K or 6000K LED?
If I remember correctly an 82c filter knocks out the blue. That way you get both. Usually possible to get a camera filter to drop over the field lens. When using filters, will there not be any loss of spectrum? The blue peak in the spectrum of 6000-6500K LEDs is especially strong. The 82C or altern...
- Sat May 27, 2023 6:26 pm
- Forum: Pictures and Videos
- Topic: radiolaria reversed
- Replies: 12
- Views: 1744
Re: radiolaria reversed
Great images !
- Fri May 26, 2023 8:22 pm
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55826
Re: Let’s talk about Delamination
I have read a number of theories as to why it occurs. Wrong type of cements, improper storage conditions, vibration/shock, the phase of the moon…in other words many guesses and no real documented evidence. Any thoughts on this? What is wrong in the "theories" ? the behavior of glass and cements und...
- Fri May 26, 2023 8:00 pm
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55826
Re: Let’s talk about Delamination
A question…is delamination worse for the viewing experience when it occurs in the eyepieces or the objectives? How about in the condenser or intermediate lens? Many condensers consist of single lenses (not doublets formed by cementing) so no delamination is expected. In principle, all glass doublet...
- Fri May 26, 2023 7:48 pm
- Forum: Microscopes and optics
- Topic: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
- Replies: 16
- Views: 3163
Re: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
Of course in the used market the buyer is at the mercy of the reseller. At Ebay the bad actor sellers required the company’s intervention since buyers quit buying after being burned. IMHO, the situation is not that bad, no need for such pessimism. I found that eBay's buyer protection is efficient a...
- Fri May 26, 2023 12:32 pm
- Forum: Microscopes and optics
- Topic: Fast repair of retraction in old Zeiss spring-loaded 40X objective
- Replies: 1
- Views: 652
Re: Easy repair of a stuck old Zeiss spring-loaded 40X objective.
So I followed the reasoning and advices posted by Apochronaut about a year ago, and removed the shroud. I wrapped the barrel and shroud, each with a strip of 5mm thick leather (taken from an old belt), gripped each with a curved (or semi-curved) 10" vise-grip (one from Franklin, Japan, the other som...
- Thu May 25, 2023 9:08 pm
- Forum: Microscopes and optics
- Topic: Fast repair of retraction in old Zeiss spring-loaded 40X objective
- Replies: 1
- Views: 652
Fast repair of retraction in old Zeiss spring-loaded 40X objective
So the spring mechanism of retraction of the 40X Zeiss Neofluar starts to fail. The barrel retracts under pressure, but stays up and must be pulled to return to normal position. In the Olympus SB objectives, the end cap serves as retainer for the spring. When the end cap is removed the spring pops o...
- Thu May 25, 2023 8:12 pm
- Forum: Microscopes and optics
- Topic: Olympus LB versus SB objectives
- Replies: 16
- Views: 6865
Re: Olympus LB versus SB objectives
When a company introduces a new product line, there is always questions from the user community concerning compatibility with existing equipment…the users want full compatibility to leverage current investments while the company wants partial to nil so to introduce new tech and sell products..and t...
- Thu May 25, 2023 8:20 am
- Forum: Microscopes and optics
- Topic: Olympus LB versus SB objectives
- Replies: 16
- Views: 6865
Re: Olympus LB versus SB objectives
Just for fun, I fitted my short barrel Olympus objectives (achromat, planapo) on the Zeiss stage. With Olympus WF10X eyepieces. Raised the slide on a small metal riser, to compensate for the 45mm vs 37mm parfocality distance. The condenser cannot reach the slide in such arrangement, so it was raised...
- Thu May 25, 2023 7:02 am
- Forum: Microscopes and optics
- Topic: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
- Replies: 16
- Views: 3163
Re: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
About " I know that the general advice is to buy something cheap first, then upgrade later " I would try to buy a good quality trinocular microscope, whether simple or sophisticated. Microscopes are precision instruments, it is part of the fun. Low quality scopes only drive one away from the hobby. ...
- Tue May 23, 2023 10:05 pm
- Forum: Microscopes and optics
- Topic: Differences/Similarities of the Olympus BH/CH scopes
- Replies: 3
- Views: 692
Re: Differences/Similarities of the Olympus BH/CH scopes
I invite a conversation discussing the differences/similarities of the Olympus BH/CH scopes. In particular I would like learn more about how compatible CH/BH versus CH2/BH2 accessories are..in particular in the phase arena…which would include the various phase condensers and short/long objectives. ...