MicrobeHunter.com Microscopy Forum

Great discussion! I believe the results of stacking have a lot to do with the subject matter. I took several stabs at producing a decent stack of cotton pollen today, and can't say I'm thrilled with any of them. Some I deleted right away. Yes, I use All Methods too, seems like it'd be silly not to h...

Even though it isn't my area, this is easily one of the most fascinating threads ever, for me! The excellent photography really helps bring the explanations to life, and makes this little unfolding drama the next best thing to visiting your lab and watching over your shoulder -- which I would dearly...

Squeezing cover glass: many years ago there used to be a mechanical device for doing this (I think it was called compressorium or something along those lines). I hope apochronaut sees this and perhaps give us more illumination on that. I'm certainly no apochronaut, but until he gets back here's thi...

Yes, very nice! Desmids are some of the most beautiful things to look at, and hardest to image well.

Very nice! I need to learn how to add a scale bar like you do - any advice?

Beautiful images! And interesting discussion - I have been experimenting with image stacks on diatoms, and have run a series or 3 image stacks and 6 image stacks, and seem to consistently prefer the 3 image stack results.

Rod, you're using Combine ZP aren't you?

I'm lost. Is the weed flattened because it's being squished, or due to effects of image stacking? And what do the cover slips have to do with it (yes I know about objective lens compensating factor)??

Very cool image - one of these days maybe I'll shoot something that's still alive...

Oh boy, can hardly wait to see what the mad scientist comes up with!

Beautiful video, as always. Can you expand a little on enhanced DF and BF?

I want to see pictures of your pipettes.

Yep, very nice image! I watch everything you post very carefully, and bet you know why.

Here ya go, an old favorite website of mine: http://snowcrystals.com/

I'm a gulf coast Texan. We take your word for it that these things exist.

I left the lens in place as discussed on my AO21 adapter discussion. I was, perhaps, a bit rash and prematurely removed that lens set. One of these days I might see about reinstalling it since I keep everything and never throw anything away like a good hoarder. :roll: Probably be best to get anothe...

75RR, good call -- it's what I shoulda done in the first place if I weren't such a lazy slob... :roll: Einman, that sure looks exactly like what I want! Phil got me running scared, sounds like there were two different sizes -- plus the eBay ad he pointed me to states 50mm diameter. As usual, however...

Yep, that's pretty serious use of a 100x oil lens alright. (I think he's cheating somehow.)

Phil, I believe that is precisely what I'm looking for. Is this the same source for such bits that you have mentioned previously? I wasn't quite aware he's in Russia. But yeah, I want to eventually end up with a piece that bayonets to my T3i, then dovetails into the photo tube. If this fits my AO ph...

That brings up a good question I have been wondering about: what do the newest big time microscopes come with these days? My Nikon E-600 and Olympus BX45 both sport 100W halogen lamps; granted, they predate LEDs by a decade or so, but what do today's equivalent stands come with (I'm not sure if they...

Thank you, all! It's a ton of fun, can't get over how many more options this new equipment gives. So here, I pull out the stops: 100x, with lens and condenser both oiled, looking at Cymbella and Pleurosigma frustules bunched together, adding the 1st order red compensator, adjusting the prism to suit...

Title says it all. I want to replace the shutter part of my AO photo tube with an adapter that bayonets to my Canon T3i. The phototube has been gutted and works fine with the camera body balanced up top, but it probably wouldn't hurt to make things a little more secure. Pictures of the fitting below...

Wait, where's the "100x diatom photo of yours?"

Proof indeed. I have seen some pretty impressive images with UV light (shorter wave length) as well - not that I would go there, as one can not view the Diatoms directly as it is extremely harmful to one's eyes. http://www.mikroskopie-ph.de/index-Diatom-3.html ^^^ Just spent an hour looking through...

While Rod keeps himself amused (shhh, don't disturb him, took me forever to get him settled down), here's a pic from today, where I was bound and determined to image some micro pores in Navicula punctuae. I think it came out pretty good ... okay, I'm thrilled with it, and believe there's still impro...

Not sure that's a good idea. Rod would probably just put a Stentor on it and kill the poor thing... :cry: Don't know if I mentioned it, but the tiny pores in the puntctua in the Navicula frustules are relatively easy to see through the eyepieces at 1000x if everything is set up nicely. After studyin...

...captures the tiniest striations or pores inside the punctuae, which I've never been able to get in an image anywhere near as clearly before this afternoon. And in fact, I have never been able to figure out if they're really physical features or optical artifacts, so if any diatom experts see thi...

75RR wrote:Nope, I asked nicely!

Just to make sure ... to "put a bug in their ear" means to "delicately suggest."

Whoa, new to me too - I wanna see! Are you gonna contact the Mic-UK admins and put a bug in their ear? (correct answer is yes)

Yep, maybe not the most startling contrast in the world, but still a ton of detail due to the oblique lighting. You make me feel silly for not pursuing it any more than I did.

Impressive alright! Besides the superb imagery, I can't imagine managing 450 photo files...

Thanks all - I'm pretty stoked! This new rig is has been a dream of mine for the past few years, and so far after just a few hours, it's everything I hoped for. With all the adjustments I have available, it becomes a matter of shooting each specimen about 5000 times because the combinations of thing...

For comparison, here are images from the same slide using the 100X Objective under bright phase contrast on my AO 10, Canon T31 with image projected directly to sensor, taken 15 May 2015. http://i70.photobucket.com/albums/i104/Arky704/Micro%20Stuff/Photomicrography/5-15-15%20San%20Saba%20Diatoms%200...