Search found 1854 matches

by charlie g
Sat Dec 30, 2023 3:04 am
Forum: Pictures and Videos
Topic: Hela Cells fluorescence DIC
Replies: 2
Views: 1641

Re: Hela Cells fluorescence DIC

Thanks for sharing this fascinating image capture. Is this microscope based at your occupation/ institution site...or do you at a home bench have this optical wonder? Please tell us about the microscopy setup, it's vintage, it's needed upkeep...do you maintain the cell line used..fascinating! Is thi...
by charlie g
Thu Dec 28, 2023 3:08 am
Forum: Miscellaneous
Topic: Snow.
Replies: 5
Views: 5096

Re: Snow.

Terrific subject, so close to my world experience..where I have lived in NY/USA. But right off..I sense it useless to state: 'each snow flake comprized of 100,000 water droplets'...why in a fascinating treatment of our historic to current understanding of ice crystals, frost, snow flakes...why throw...
by charlie g
Mon Dec 25, 2023 11:07 pm
Forum: Beginner's corner
Topic: Best size objective for Pond life?
Replies: 36
Views: 58146

Re: Best size objective for Pond life?

Merry Christmas, Joe Henry, and group. Encouragement is the 'coin of the realm' in this microscopy forum ( again, thank you Oliver Kim, for setting this community up and thriving). Remember simple attention to your wet-mount slides..the sandwich of: your microscope slide+ the amount of sample fluid ...
by charlie g
Mon Dec 25, 2023 5:13 am
Forum: Illumination Techniques
Topic: Does phase contrast make sense for its price?
Replies: 49
Views: 34029

Re: Does phase contrast make sense for its price?

Desmid, and hydra with dl-phase contrast method.
by charlie g
Mon Dec 25, 2023 4:29 am
Forum: Pictures and Videos
Topic: ADi source of radiolarian slides
Replies: 2
Views: 590

Re: ADi source of radiolarian slides

Stephano Barone/ the Diatom Shop offers a terrific 'test slide' to calibrate, to understand performance of your stands optics.

I enjoy the radiolarian fixed slides, and diatom fixed slides from Stephano. Visit his "Diatom Shop". charlie g.
by charlie g
Mon Dec 25, 2023 3:47 am
Forum: Illumination Techniques
Topic: Does phase contrast make sense for its price?
Replies: 49
Views: 34029

Re: Does phase contrast make sense for its price?

Hi, osterport, and group. Visual observations with Nikon dl phase finite (circa 1980's?) optics: 10X, 20X, 40X, 60X, and 100X-oil objective..are terrific. My crude image-capture/ camera setup never records what visually I enjoy with dl-phase contrast methods. And the variety of Nikon phase condenser...
by charlie g
Sat Dec 23, 2023 4:32 am
Forum: About the website, the magazine and this forum
Topic: New trouble uploading images...
Replies: 19
Views: 16970

Re: New trouble uploading images...

I tried multiple times to PM, admin(Oliver)...none of my PM's launched. Oh well, Merry Christmas all/ happy Holidays..and let's enjoy a peaceful and healthy 2024


charlie guevara/ finger lakes, US
by charlie g
Sat Dec 23, 2023 4:17 am
Forum: Beginner's corner
Topic: Best size objective for Pond life?
Replies: 36
Views: 58146

Re: Best size objective for Pond life?

Hi, all, many non-mobile, or low mobile ( diatoms, amoebae, etc.) are fascinating targets for : 60X, 100X oil-objectives. For those active protozoans..learn to use methylcellulose 1.5%/ half pondwater/half this methylcellulose viscosity tool. We need to learn how to keep the water column/ water film...
by charlie g
Fri Dec 22, 2023 2:47 am
Forum: About the website, the magazine and this forum
Topic: New trouble uploading images...
Replies: 19
Views: 16970

Re: New trouble uploading images...

Here tonight about 9:30pm-9:45pm..none of my images upload into my own topics, or into a reply to another members thread. charlie g
by charlie g
Fri Dec 22, 2023 2:32 am
Forum: Pictures and Videos
Topic: ADi source of radiolarian slides
Replies: 2
Views: 590

ADi source of radiolarian slides

Hi ADi, and group..here is Stefano Barone/ Italy source I have recieved excellent prepared slides from. I tried to put these images into my post into ADi's "Rocking Radiolarians" thread..I was not able to place images into my post into ADi's thread..is this a new policy to address rude boys like me?...
by charlie g
Fri Dec 22, 2023 2:05 am
Forum: Pictures and Videos
Topic: rocking radiolaria 50x
Replies: 17
Views: 11663

Re: rocking radiolaria 50x

Beautiful, elegant microscopy...thank you, ADi. Reminds me of 1934 comic evil villain/ nemesis of heroic Flash Gordon. I can see 'merciless Ming's helmet as your radiolarian. Can you give the source for radiolarian 'frustule' ? Can you give the objective used for these beautiful images? I have been ...
by charlie g
Fri Dec 22, 2023 1:22 am
Forum: About the website, the magazine and this forum
Topic: New trouble uploading images...
Replies: 19
Views: 16970

Re: New trouble uploading images...

Hi again, group, a few days ago I could not download issues of: "Microbehunter Magazine"..I was only allowed to "purchase printed copies of any selected issue"..none of the series permitted me to download the magazine ( as I used to do all the time). I wanted to download magazine issues in which I c...
by charlie g
Thu Dec 21, 2023 3:37 am
Forum: Beginner's corner
Topic: Best size objective for Pond life?
Replies: 36
Views: 58146

Re: Best size objective for Pond life?

I suggest, as does Phil/apo, learn to use your oil-100X objective..be selective..do what you enjoy with 20X, 40X, 60X dry...save 100X-oil for last excitement of your designated treat organisms on a wet mount slide. As was suggested already..larger rectangular coverslips permit at least 80% of your w...
by charlie g
Mon Dec 18, 2023 3:11 am
Forum: Specimens, samples and slides
Topic: Protist aquarium- how long should I run air pump
Replies: 11
Views: 6351

Re: Protist aquarium- how long should I run air pump

Hi again, Fan and forum. First off I apologize for not understanding how to delete one of my exactly the same 'double posts'..how to delete one? I need advice to do this deletion. Yes, yes, Fan..you ask about 'biodiversity of your desired protists' , you ask which more 'biodiverse'..a water heated t...
by charlie g
Fri Dec 15, 2023 4:15 am
Forum: Specimens, samples and slides
Topic: Protist aquarium- how long should I run air pump
Replies: 11
Views: 6351

Re: Protist aquarium- how long should I run air pump

Hi, Fan,..first off..'think native/ natural assemblages from your Texas outdoors. Thus..please, please, no water-heater!'. A water heater is akin to a bacterial incubator..strive for for natural/native freshwater assemblages. Second...think ten (10) to fifteen (15) gallon tank...this permits a tray ...
by charlie g
Fri Dec 15, 2023 4:13 am
Forum: Specimens, samples and slides
Topic: Protist aquarium- how long should I run air pump
Replies: 11
Views: 6351

Re: Protist aquarium- how long should I run air pump

Hi, Fan,..first off..'think native/ natural assemblages from your Texas outdoors. Thus..please, please, no water-heater!'. A water heater is akin to a bacterial incubator..strive for for natural/native freshwater assemblages. Second...think ten (10) to fifteen (15) gallon tank...this permits a tray ...
by charlie g
Fri Dec 08, 2023 3:14 am
Forum: Pictures and Videos
Topic: An enjoyable first sample of 12/2023 outdoor mesocosm
Replies: 5
Views: 2070

Re: An enjoyable first sample of 12/2023 outdoor mesocosm

I sense these inclusions nested within this Actinosphaerium amoeboid protozoan are not engulfed food...it would be fun to keep these large protozoans in a well slide..and follow up opn what becomes of these inclusions. The high magnification image suggests these inclusions are endosymbiotic dinoflag...
by charlie g
Fri Dec 08, 2023 3:07 am
Forum: Pictures and Videos
Topic: An enjoyable first sample of 12/2023 outdoor mesocosm
Replies: 5
Views: 2070

Re: An enjoyable first sample of 12/2023 outdoor mesocosm

Please note image capture #184 depicts a hydra with six tentacles, the other image captures are of a five tentacle hydra.
by charlie g
Fri Dec 08, 2023 2:59 am
Forum: Pictures and Videos
Topic: An enjoyable first sample of 12/2023 outdoor mesocosm
Replies: 5
Views: 2070

Re: An enjoyable first sample of 12/2023 outdoor mesocosm

The two meiofauna/worms show very clear gross anatomy...their mouths open and close at times.
by charlie g
Fri Dec 08, 2023 2:48 am
Forum: Pictures and Videos
Topic: An enjoyable first sample of 12/2023 outdoor mesocosm
Replies: 5
Views: 2070

An enjoyable first sample of 12/2023 outdoor mesocosm

Hi all, happy holidays to all. A simple wide mouth plastic jar scoop sample of my outdoor pond/stream mesocosm ( it's been going for years now..red squirrels punctured 2/3rds of the 38 ft length stream component water liner, winter 2022...sighh) on the very first wetmount slide I slurped up from the...
by charlie g
Thu Dec 07, 2023 11:15 pm
Forum: Identification help
Topic: Is this Paramecium multimicronucleatum?
Replies: 6
Views: 3207

Re: Is this Paramecium multimicronucleatum?

Thanks for an interesting thread, 'nachoben'...and hear, hear thankyou, Bruce, for your postings here, and those PubMed links regarding P. bursaria, and the 'other paramecium with internal symbiotic algae'. I agree with Bruce: 'it is enriching even to get to genus level grasp' of so many of our prot...
by charlie g
Sun Dec 03, 2023 11:57 pm
Forum: Miscellaneous
Topic: A discussion about experience
Replies: 39
Views: 35024

Re: A discussion about experience

Respectfully, Frankiev, there is a segment : 'Off Topic' in this microscopy forum...in wonderful astronomy forum: "Cloudy Nights.com"..there is a similar: 'off topic forum'. You need not announce your 'out of here'/ ' your gone.'...but please consider the wonderful knowledge this thread alludes to.....
by charlie g
Sat Dec 02, 2023 5:34 am
Forum: Miscellaneous
Topic: A discussion about experience
Replies: 39
Views: 35024

Re: A discussion about experience

Please be patient with yourself, Don, these are exciting times for pondering the theory of mind, the theories of consciousness/ self consciousness. " Putting Ourselves Back in the Equation: Why Physicists Are studying Human Consciousness and AI to Unravel the Mysteries of the Universe", George Musse...
by charlie g
Tue Nov 21, 2023 2:54 am
Forum: Microscopes and optics
Topic: Is a 2x objective worth having?
Replies: 11
Views: 5509

Re: Is a 2x objective worth having?

Yes, yes, osterport...your 2X microscopy of that insect humbles me with all that gross anatomy/ morphology in the image captures ( hear, hear for Andy Chicks low cost text: " Microscopy of Insects".) . Thank you, OP, for this thread...a Plan 2X objective is so much more then a 'scan the slide for ta...
by charlie g
Mon Nov 13, 2023 6:22 am
Forum: Microscopes and optics
Topic: Just bought a set of plan objectives
Replies: 11
Views: 2324

Re: Just bought a set of plan objectives

Hi, scarodactyle,...you assume a seller is oblivious to the market said seller pitches items for sale in? So said seller , when offering items for sale 'locally, and over seas'...this seller can be silent on 'trap door fees beyond sellers control'? This is exactly why I am avoiding purchases from sp...
by charlie g
Mon Nov 13, 2023 5:53 am
Forum: Microscopes and optics
Topic: Just bought a set of plan objectives
Replies: 11
Views: 2324

Re: Just bought a set of plan objectives

Hi, micropunter and all, I here in con-US dropped out of a fantastic gift clothing deal for my spouse, son, and others...wow, the company is located Shanghai/abroad..lead me to click on purchase prices...then came surprize step of: 'insurance fee', then came 'shipping cost'..!! I dropped off this fe...
by charlie g
Mon Nov 13, 2023 5:10 am
Forum: Specimens, samples and slides
Topic: Water Sample Disposal
Replies: 6
Views: 3899

Re: Water Sample Disposal

I agree with many fellow posters: nourish your indoor potted plants with these 'imported area treats', or out doors on your grasses or bushes. I strongly disagree with useing your kitchen sink, or toilet...a matter of keeping your silverware and dishes far from collected outdoor samples...and keepin...
by charlie g
Wed Nov 08, 2023 2:14 am
Forum: Microscopes and optics
Topic: Is a 2x objective worth having?
Replies: 11
Views: 5509

Re: Is a 2x objective worth having?

Is it true, Phil Brown, that 'horologist' ( as opposed to : 'clock maker/ clock repair')...is it true that our word for unit of time: ' the hour'..is related to our word: horologist? My dad repaired fine wrist watches in NYC/ 'diamond district'. My thanks to OP for asking about use of a 2X objective...
by charlie g
Tue Nov 07, 2023 4:34 am
Forum: Microscopes and optics
Topic: Is a 2x objective worth having?
Replies: 11
Views: 5509

Re: Is a 2x objective worth having?

Hi all, my 'work horse stand' is a Nikon Labophot Pol 'clinical microscope' finite 160 mm stand...circa 1980's??. This stands 1.25 na substage condenser has a turrent of: BF, DF, Ph1, Ph2, Ph3, Ph4 settings. My Nikon Plan 2X works well with both darkfield, and bright field...I do have to 'stand on m...
by charlie g
Sun Nov 05, 2023 2:12 am
Forum: Microscopes and optics
Topic: Is a 2x objective worth having?
Replies: 11
Views: 5509

Re: Is a 2x objective having?

Hi, for my wetland and freshwater pondlife...my Plan 2X is excellent for many of the larger plants and animals. Yes, when my targets are protozoa and smaller algae, the 2X objective is a 'scan tool'...but it is essential for larger plants and the colonies organized on these plants...same goes for co...