MicrobeHunter.com Microscopy Forum

Thanks Hans. Yes chasing rotifers around is like herding cats

More scouring of books and online has yielded nothing. Chalking this one down as "Fungal?.. Weird, but move along"

Lovely images adomacro, you obviously put a lot of time and effort into your work.

I recently ran into this same problem. I bought a second Vickers M17 for the 1.25na achro' oil condenser and a second objective changer. The condenser is great but would not provide even full-field illumination for objectives below 6.3x and required swapping to to the original fip-top condenser for ...

Not sure, definitely some bacteria present. Detritus and brownian motion?

A new rotifer found in the microbe aquarium. Powerful feeding currents. In the latter half of the video you can see the dorsal antenna being used to sense nearby dangers.
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Microscope: Vickers M17 trinocular
Objectives used:
Zeiss Jena 6.3x Apo
Zeiss neofluar 16x
Zeiss Jena 25x Apo

Seems a bit too uniform and in one plane for it to be fungal, can't rule it out though.

Not sure if this is a worm or nematode (other?), but unable to find anything similar on the web. Lives life at full throttle, observed for some time but wouldn't stay still for a decent shot. Odd behaviour, freed itself from this clump of detritus only to get entangled in another, and another, and s...

Shotcut for me also. Nice work Hans, hopefully you can cure the banding issue.

DonSchaeffer wrote: ↑

Sun Nov 15, 2020 11:31 pm

How are they eaten? Where is the "mouth?"

Hydra are essentially a hollow tube (blind gut), the feeding entrance is in the middle of the tentacle cluster. They can engulf large prey in short order. Here is a good video (not mine) of one in action: https://youtu.be/SS_HYY97ehw

Give it a while, thingiverse is slow to display uploaded designs these days

Scraper for collecting algae samples from flat sided aquariums and culture vessels. Useful for collecting encrusted flora/fauna for study. Slide the scraper vertically up the tank side with firm pressure (scoop section downmost), scrapings collect in scoop and can be rinsed out for examination. Made...

Thanks for the encouragement janvangastel

Nice video. Sort of looks like there are bulges of clear fluid swelling around the ciliate in the bright field shot 2:45 to 3:45. Any idea what that is? Related to the damage from the hydra? Thanks hans. I think this is cytoplasm leakage caused by the ciliate triggering one or more of the hydra's n...

Good work, well done. I've noticed these rest states too. Not sure if its due to lowered oxygen content under the coverslip, or just a behavioural trait?

Brown Hydra flexing. Wrong time and place for the Ciliate.
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Microscope:
Vickers M17 trinocular
Objectives:
Vickers Microplan 4x
Vickers Microplan 10x
Zeiss neofluar 16x
Zeiss Jena APO 25x

MichaelG. wrote: ↑

Sun Nov 15, 2020 10:01 am

Monochromatic lighting is fundamentally superior to post-processing

MichaelG.

Unless what you want to resolve emits the same wavelength as your monochromaticity?

Very useful, thanks.

If your camera can shoot in RAW format the Camera Raw plugin for Photoshop has a 'remove chromatic aberration' feature. The colour range of the aberration you wish to remove and intensity can be set manually. Obviously only applicable for Photoshop users with a RAW capable camera, but worth mentioni...

Amoeba? Possibly Nuclearia or Vampyrella.

You mention in your previous thread that the image isn't par-focal with this photo adapter, and that it was only par-focal if you elevated it further (without eyepiece?). I'd suggest you try elevating it temporarily and see if the hot spot goes away.

I experimented with many LED solutions as a replacement source for the 100w halogen Köhler illumination on my Vickers M17. Inserting a diffuser at the light source does have a small impact on contrast on my scope (white balanced). This might not be the case on other scopes. If you are considering a ...

Nice work Bogdan, very cool

Tired of arranging diatoms?
https://www.independent.co.uk/life-styl ... 88066.html

The new generation AD1605 4k microscopes look very promising but prices (£500) are approaching that of a decent used (or new chinascope) trinocular stereo microscope, and you will still need to DIY your own transmitted illumination to be comparable. The AD407 based units seem to be the sweet spot fo...

Greg. If it bothers you stick to studying inert materials. Better yet, lock yourself away in in a sterile enclosure away from any microbes you may harm from leading a normal life. Remember not to bathe lest you destroy millions of helpless microbes daily. Don't worry, in a few days when you die (you...

My main setup is 6.3x 16x 25x 40x 50x(oil), 25x is by far the most used magnification once the subject has been spotted, 6.3x I find ideal for searching. 4x used mainly for larger crustaceans and plant sections.

I have a small bubbler running on a timer 12hrs on 12hrs off. For my tank I find this keeps the alae growth at a good level and prevents any stench. Whatever methods you go for its worth giving some consideration on how best to extract the microorganism samples for viewing. I recently added a couple...

Nice detail on the mouthparts Bob, not easy chasing those around under the scope.