Search found 3154 matches

by MicroBob
Tue Jun 11, 2019 2:00 pm
Forum: Illumination Techniques
Topic: PZO DIC with MPI-3 and KPI-2
Replies: 18
Views: 12391

Re: PZO DIC with MPI-3 and KPI-2

Hi Phil, from what I know the head from the ebay link is the MPI-5 with a different type of lever and some other differences to the MPI-3 and MPI-2 heads. My DIC head is really named MPI-3. I have seen complete kits with box that include one head and two condensers, slit and revolver. This would pro...
by MicroBob
Tue Jun 11, 2019 9:18 am
Forum: Resources (online, books etc.)
Topic: R.M.S. die.
Replies: 22
Views: 18223

Re: R.M.S. die.

Hi Michael, 0,003 inch tolerance is 0,0762mm, this is actually a comparatively coarse tolerance for todays standards. At the time this standard was defined the lathes will have looked "somewhat" different though. I couldn't find a date for this standard. Maudsleys invented the lead screw around 1800...
by MicroBob
Tue Jun 11, 2019 8:17 am
Forum: Illumination Techniques
Topic: PZO DIC with MPI-3 and KPI-2
Replies: 18
Views: 12391

PZO DIC with MPI-3 and KPI-2

Hi together, I place this in a separate thread: From PZO there are upper DIC units named MPI-2, MPI-3 (the one I have) and MPI-5 (looks different than the two before). The revolver condenser seems to be always KPI-2. In the internet I found a description of the newest system for the Biolar, with MPI...
by MicroBob
Tue Jun 11, 2019 7:43 am
Forum: Resources (online, books etc.)
Topic: R.M.S. die.
Replies: 22
Views: 18223

Re: R.M.S. die.

Hi Phil, cutting the thread with a die would lead to quite big cutting forces. I'm not sure that the barrel really is intended to withstand them. On the other hand side sigle point cutting on the lathe is a very fine process when done in several cuts (and always stopped before the shoulder). For thi...
by MicroBob
Mon Jun 10, 2019 8:26 pm
Forum: Resources (online, books etc.)
Topic: R.M.S. die.
Replies: 22
Views: 18223

Re: R.M.S. die.

Hi Phil, here RMS taps and dies are offered: https://www.baercoil.com/pdf/Gewindebohrer2014EN.pdf Price on request, propably dirt cheap! :mrgreen: I don't know what quality would be to expect there. It isn't an established manufacturer, maybe better chinese quality? Are you up to an interesting proj...
by MicroBob
Mon Jun 10, 2019 6:22 pm
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 68
Views: 39489

Re: dIY DIC

Hi together, concerning PZO DIC: There are upper DIC units named MPI-2, MPI-3 (the one I have) and MPI-5 (looks different than the two before). The revolver condenser seems to be always KPI-2. In the internet I found a description of the newest system for the Biolar, with MPI-5, KPI-2 and additional...
by MicroBob
Mon Jun 10, 2019 6:01 pm
Forum: My microscope
Topic: Olympus VT-II Stereo Microscope
Replies: 7
Views: 8254

Re: Olympus VT-II Stereo Microscope

Hi Bryan, congratulations to the new stereo microscope! You will probably find lots of uses for it. I would really really miss mine, at home and at work. I have an (I think) older Olympus model, the nuclear reactor design type. I use it with 10x eyepieces which resolve everything the objectives have...
by MicroBob
Mon Jun 10, 2019 7:27 am
Forum: Beginner's corner
Topic: First Stain, and mounting
Replies: 7
Views: 4216

Re: First Stain, and mounting

1. Is it normal to have the cover slip rock? The slides I used were beveled edge flat slides. 2. Is there a way to get the stain to penetrate into intact cells? 3. Once finished I assume I clean and reuse both the slide and cover slip? 4. I noticed that the stage movement controls on the Microscope...
by MicroBob
Sun Jun 09, 2019 7:10 pm
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 68
Views: 39489

Re: dIY DIC

Hi Fan, I have just assembled a PZO DIC set consisting of revolver condenser and MPI-3 (?) head. For a while now I am working on my office/lab room and work is not yet finished, most things are covered and not available. So I only had a quick try with the set and a 1960s cream white PZO MB 30 with s...
by MicroBob
Sun Jun 09, 2019 6:05 am
Forum: Specimens, samples and slides
Topic: Slide mounting media for a beginner - observations
Replies: 24
Views: 15055

Re: Slide mounting media for a beginner - observations

Hi John, your slide making is on an extraordinary quality level and I always enjoy your posts where you openly explain your methods! For the beginner it is good to understand that scientific value and personal enjoyment can already be gained from slides that are very crude compared to what the best ...
by MicroBob
Sun Jun 09, 2019 5:22 am
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 68
Views: 39489

Re: dIY DIC

[quote="zzffnn"]DIY oblique gets difficult over 40x NA 0.65 and does not produce the same contrast DIC produces. My own (years of) test results showed that DIY oblique can provide decent visual 3D effects at high power, but such effects cannot be easily captured with camera. So for me, if I were to ...
by MicroBob
Sat Jun 08, 2019 8:47 pm
Forum: Specimens, samples and slides
Topic: Slide mounting media for a beginner - observations
Replies: 24
Views: 15055

Re: Slide mounting media for a beginner - observations

For me it is important to learn to prepare slides, be it temporary or permanent. When microscopy consists of observing a set of ready made slides interest will faint soon. (Unless you have a really big slide collection of cause). It is like 3D-printing without the ability to design ones own 3D-model...
by MicroBob
Sat Jun 08, 2019 8:29 pm
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 68
Views: 39489

Re: dIY DIC

Hi Heather, these filter tricks work differently well with different microscopes. Also it is always necessary to do some fiddling to get the best image so repeatability or even quick repeatability is not really available. On the other hand side a real DIC setup is also difficult (and expensive!) to ...
by MicroBob
Sat Jun 08, 2019 5:22 am
Forum: Beginner's corner
Topic: Which Microscope?
Replies: 28
Views: 18535

Re: Which Microscope?

Hi Dave, I have a pair of binoculars from Bresser, 8x20, model "Corvette",new price 90€, was tested by "Stiftung Warentest" with result "good". The lenses are made in Japan. It is actually quite good and has costs me just 8€ including shipping used on ebay. So there are good products in their lineup...
by MicroBob
Fri Jun 07, 2019 7:48 pm
Forum: Beginner's corner
Topic: Which Microscope?
Replies: 28
Views: 18535

Re: Which Microscope?

Hi Dave, it is nice to see that they both stick to sensible magnifications - this inspires a lot more trust in the usability of the microscope. The Apex includes a nice compact travel case which would be very nice to take the instrument with you on trips or to meetings. I wouldn't trust too much in ...
by MicroBob
Fri Jun 07, 2019 7:18 pm
Forum: Specimens, samples and slides
Topic: Slide mounting media for a beginner - observations
Replies: 24
Views: 15055

Re: Slide mounting media for a beginner - observations

Hi Heather, thank you for sharing your experiences with mounting media! There is much room for new solutions, especially widely available ones. Some thoughts about the media you use: -Hoyers medium: It lightens some specimens over time, e.g. whole insects. One component makes it dangerous to swallow...
by MicroBob
Fri Jun 07, 2019 1:37 pm
Forum: Microscopes and optics
Topic: A new [to me] word learned today
Replies: 6
Views: 2629

Re: A new [to me] word learned today

http://www.microscope-antiques.com/browningplaty.html Just for info. I scanned the advert from an excellent little book, recently purchased 'Through a Pocket Lens' by Henry Scherren F.Z.S. second impression, 1904 MichaelG.[/quote] Hi Michael, thank you for the link! There are some for sale on ebay ...
by MicroBob
Fri Jun 07, 2019 1:32 pm
Forum: Off Topic
Topic: Micscape online Magazine
Replies: 27
Views: 26769

Re: Micscape online Magazine

One big advantage of sites like Micscape is that interesting information is archived and easy to find. In forums the interesting part is often difficult to find and time consuming to extract. My impression is that at the beginning of private internet in the mid-90s much information was presented as ...
by MicroBob
Wed Jun 05, 2019 11:17 am
Forum: Miscellaneous
Topic: Chinese inspection camera
Replies: 10
Views: 6251

Re: Chinese inspection camera

Hi 75RR, may be I have missed it but I only saw the typical exepiece cameras. With inspection camera I connect something small and endoscope like. What I always find interestig is the way the english clubs organize their meetings: The meetings seem to consist of separate exhibits and flea market sta...
by MicroBob
Tue Jun 04, 2019 6:53 pm
Forum: Specimens, samples and slides
Topic: Tried glycerin to slow down fast movers
Replies: 36
Views: 26183

Re: Tried glycerin to slow down fast movers

I've got a hand crank centrifuge for two quite big glasses, ca. 15x100mm. This will probably do. Otherwise we have an electric one in our group lab available. Centrifuging is a nice process step, not messy, no fumes and no waste. Thank you for showing these filters, Doron! I have heard of membrane f...
by MicroBob
Tue Jun 04, 2019 4:36 pm
Forum: Specimens, samples and slides
Topic: Tried glycerin to slow down fast movers
Replies: 36
Views: 26183

Re: Tried glycerin to slow down fast movers

I ended up spinning it very fast which made a ton of difference, I could see all the crap pelleted at the bottom and a very nice clear solution on top which I collected and placed in a separate vial. It seems clean looking at it through the scope. Hi Wes, so you first centrifugated first and then f...
by MicroBob
Sun Jun 02, 2019 5:13 pm
Forum: Pictures and Videos
Topic: Sodium ascorbate
Replies: 6
Views: 4180

Re: Sodium ascorbate

Hi Wes,
beautiful crytal image!
I always find it astonishing that even these seemingly flat crystals have a depth structure and hills and valleys.

Bob
by MicroBob
Sun Jun 02, 2019 1:19 pm
Forum: My microscope
Topic: IOR ML-4M
Replies: 21
Views: 15073

Re: IOR ML-4M

Hi Farmazon, I would suggest a different adaptation over a pancake camera lens like the old Pentax M 40mm 1:2,8 for APS C. The picture shows a Nikon 1J5 with 18.5mm lens directly adapted to an eyepiece for glasses wearer. This is much shorter and I can just pull out an eyepiece of the bino tube and ...
by MicroBob
Sat Jun 01, 2019 8:53 pm
Forum: Pictures and Videos
Topic: My first diatom stack
Replies: 14
Views: 5679

Re: My first diatom stack

Hi Doron, this is what I would expect and I wouldn't worry about 1%. Fit for purpose, acceptable price and available for everybody. It is great to have a common test plate that arrives at you doorstep without hunting ebay auctions etc. To save costs the manufacturers tend to just barely deliver what...
by MicroBob
Sat Jun 01, 2019 5:40 pm
Forum: Pictures and Videos
Topic: My first diatom stack
Replies: 14
Views: 5679

Re: My first diatom stack

75RR wrote:Very happy with it.
There it is: A satisfying chinese product! :D If one could depend more on the quality it would become difficult for us to compete...
I have a chinese object micrometer: Also very satisfying for my needs (haven't checked the absolute accuracy)

Bob
by MicroBob
Sat Jun 01, 2019 12:02 pm
Forum: Pictures and Videos
Topic: My first diatom stack
Replies: 14
Views: 5679

Re: My first diatom stack

Here is a PDF with eyepiece reticules: https://drive.google.com/open?id=1bVYdt_zQeBmGGidVmVnlIJo6722zLXRQ It works with 80% of the eyepieces I tested. In most cases there is a convenient spot to put the reticule on. Real measuring eyepieces provide focusing to get a perfectly sharp image of the reti...
by MicroBob
Sat Jun 01, 2019 7:26 am
Forum: Pictures and Videos
Topic: Shell picking from Red Sea sand
Replies: 45
Views: 19804

Re: Shell picking from Red Sea sand

are even used to direct sideways drilling Hi 75RR, 25 years ago I applied for a job in a copmany producing drilling equipment. At this point of time they communicated with the drilling head by means of pressure pulses in the water supply to the head. It's fascinating to hear that foraminifera are s...
by MicroBob
Sat Jun 01, 2019 7:22 am
Forum: Pictures and Videos
Topic: My first diatom stack
Replies: 14
Views: 5679

Re: My first diatom stack

Wes wrote:but not an eyepiece graticle.
Hi Wes, you can print one with a laser printer on overhead projection film. Under magnification these laser prints don't look too nice but as a scale this works acceptably. Ink jet might even be better.

So there is no reason to deny us the measurement! :mrgreen:

Bob
by MicroBob
Sat Jun 01, 2019 6:58 am
Forum: My microscope
Topic: IOR ML-4M
Replies: 21
Views: 15073

Re: IOR ML-4M

The parfocal business is driving me up the walls. I have (now) a manufacturer's photo tube with projection eyepieces, but i can't achieve parfocality whatever i do. Varying the distance between the camera and the mount - the tube length does nothing with respect to focus. The image is still defocus...
by MicroBob
Fri May 31, 2019 4:35 pm
Forum: Pictures and Videos
Topic: Shell picking from Red Sea sand
Replies: 45
Views: 19804

Re: Shell picking from Red Sea sand

Does digging deeper into a beach sand bank offer ancient forams? Hi Charlie, foraminifera can be used to research the movement of coast lines. When you bore a hole with a hollow drill you can part it in layers, clean it ( I think with H2O2) and look whether and which foraminifera are there. Our mic...