MicrobeHunter.com Microscopy Forum

Thanks for the link 75. Very interesting article.
Tom W.

Amazing! That last image really shows the "undulatum" feature very well.
Tom W.

Ahhhh... thank you 75RR. Of course they are bees, now that I look at them more closely They still seem familiar, but I'll just put that down to my continuing decline into senility.
Tom W.

Another beaut 75RR... very nicely done.
Changing the topic slightly, can you tell us about your little fly logo? I have this strong feeling I've seen it before somewhere, possibly in connection with a mathematical problem, but that might just be my geriatric mind wandering!
Tom W.

I'm with Wes. Let's hear the details. Very nice result.
Tom W.

Wonderful image. That 63x just never wears out!
Tom W.

Welcome scottybee from another Ontarian. I'm sure you'll find this a worthwhile place to hang out.
Tom W.

Excellent!!!!!!!!!!!!!!!!!
Tom W

Depending on your intended use, if you decide to try silicone sealant, you should be careful about which type you use. Most sealants intended for household use, e.g. bathtub sealant etc., are compounded with antifungals, which can be harmful to living organisms. There are sealants made without these...

Awesome shots Wes!
tgss

Very nicely done Bob. I'll definitely be following you along on this journey.
Tom W.

Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.

Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.

Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.

+1 on the Ortho-Illuminators - very, very sexy!
Tom W

Thanks for the update on Part 4 of the series Kurt. I have the other three, but wasn't aware that Tony had released a fourth. As you stated, the books are a terrific source of thoroughly practical ideas and procedures, with attractive and informative illustrations. Tony is a bit of a Vickers M41 fan...

It's apparently available on Amazon Prime video, to rent - $4.99, or buy - $5.99, if that helps anyone.

Tom W.

Hi 75RR I'm beginning to feel guilty about hijacking Radazz's post with this but... I agree that you need to know what the pixel size really is in order to compare it with what is needed. I guess, for me at least, the discussion boils down to the recognition that, in order to figure out what pixel s...

Hi 75RR I think the misunderstanding is because I used an unfortunate term, pixel density, since it implies pixels/mm^2, or some such, whereas I was using it the sense of "number of pixels/sensor". That's because I'm an idiot. :oops: The intent was to get around the issue of different sized sensors ...

The pixel size should be 3 or 4 times smaller that the projected size of the object you are trying to capture. See link and the extract below: https://www.lumenera.com/blog/understanding-resolution-in-digital-microscopy please note that when they say: " largest possible pixel size required to fully...

I've been a little perplexed while following this thread. The idea that resolution depends primarily on pixel size is the idea that's causing me problems. I admit that "primarily" has not been explicitly stated, but seems to be implicit in the arguments. If carried to extremes this would imply that ...

Well... I'm gobsmacked! I couldn't understand what was going on when I read billbilt's posts - thought there must have been some kind of malfunction on the forum, until I read the following posts and realised it was for real. Very, very disappointed - really undermines the forum and leaves a bad tas...

Very unfortunate that this instrument was trashed Piter_. I wish you every success in restoring it to fully working condition.
Tom W.

What mrsonchus said .

Tom W.

I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calculate the number of pixels per graduation to generate a scale bar. Thank you, Wes, for the clarification. No need to generate...

Fascinating stuff Wes, excellent images and a very clear exposition of the physics of the process. Can you give an idea of the scale of the images, or at least the objective powers used?
Thanks for sharing.
Tom W.

Hi Aslan You might take a look Electron Microscopy Sciences. In particular https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx#17997 . They are based in the states, but I have ordered from them in the past and received very good service. Many of their products, though apparen...

Looks like this might be very interesting. Thank you for the link.
Tom W.

I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a ...

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now. Tom W. This is the one I b...