MicrobeHunter.com Microscopy Forum

More pics...

Not too much activity in my sample that I took from a dewatering channel, but diatoms are always nice to observe.... https://up.picr.de/37969452vs.jpg https://up.picr.de/37969453jr.jpg https://up.picr.de/37969454rt.jpg https://up.picr.de/37969455sm.jpg https://up.picr.de/37969456dv.jpg https://up.pi...

Congratulations!

Found a comparision dry, water and oil immersionare here: https://www.mikroskopie-forum.de/index.php?topic=4350.msg28437#msg28437 Comparison with Pleurosigma angulatum and Nitschia sigma (on the left photos immersion with water, on the right with oil: https://www.mikroskopie-forum.de/index.php?topic...

Speaking of FOV I replaced my Leitz 1x objective turret with the 0.8x, combined with the Fluotar objectives and the 30MM 10x/26 eyepieces, the FOV is incredible. There is a point where it is probably overkill as I find my eyes wandering around-LOL. Some may find this interesting in context of the F...

Top!

On my BX60F3 the U-AN analyzer works fine for transmitted light and DIC.

Hi Zero,
the adaption of a DSLR to your BX60F can be done rather cheap: just a tube without any optics:
viewtopic.php?f=24&t=5930&start=60#p73297.
The intermediate image of the microscope fits the size of DSLR with APS-C sensor size.

microb wrote: ↑

Wed Jan 22, 2020 11:23 pm

And does it need to be adjusted each time the turret is changed?

BX: If you change the objective you need to turn the condensor wheel on step further for the correct match of the DIC prism in the condensor. Since the condensor wheel-turret turns easily and smootly, I have not an issue with that.

Hi,
like Bob stated, delamination damage for the PlanApo 100/1,3 is extremely common. Difficult to argue on later price discounts for complete sets that are sold as not tested. And without any light source you cannot even blame the seller, since the microscope was not in working condition.

There are various different table inserts available for the vertical Zeiss IM35. Recently I had the chance to get another table insert, it is another variant of a holder for petri-dishes, the Zeiss item number is 47 17 38. The holder is adjustable, the claws of the holder allows the use of different...

I can recomment the below guideline "Carl Zeiss MicroImaging - The Clean Microscope".

https://bio.sabanciuniv.edu/sites/bio.s ... cope-1.pdf

Thanks for sharing, also enjoyed the 8x time lapse at the end of the video sequence!

I am a big supporter for the Zeiss Inverted IM35 series ... they are research grade instruments and can be equipped with components from the Zeiss Standard series and there is really a big second hand market. I would already have bought the below scope ... but it gets too much if you add transportat...

Hi 75RR, you are right, the tube is not commerically availble, but there is a guy in the Austrian micro forum who fabricates such tubes. Anyway it is not rocket-science, one side connects to the tube head an the other side has the thread for the T2 adapter for Canon bajonet. The phototube itself is ...

Would like to share some details for a very compact and simple photo adaption for the Olympus BX: Photo adapter length is just 60mm / 2,4 inches (distance from the trino flange to the Canon EOS Camera flange) The intermediate picture of the microscope is directly projected on the sensor of the Camer...

Congrats, really nice foto.

Great Photos 75RR!

Maybe you need to try one of the older Zeiss Objectives with n.A. of incredible 1.6:
PlanApo 100/1,6 Imm or the 74/1,6

Nice capture Chris. Thanks for sharing.

Hi Hobbyst46, definitely the Zeiss Standard still performes very well, respectively if equipped with Planapos - and yes it will cost only a fraction of the invinite systems. From my experience I do not think that the SKPL 10x/20 makes a much better performance compared to a KPL 10x/20. So it is a ni...

Hi, did a comparison of my photo adaptions I use currently: green rectangle: Olympus BX with Canon EOS600D (the intermediate image is directly projected to the camera sensor) red rectangle: Zeiss Standard with large field optovar 0,8x - KPL SKPL 10x/20 (Ocular) - YN EF35mm (Camera objective) - Canon...

What i recognized in an instant when mounting the 0,9 condenser: The image of the field aperture is so colourful and unsharp that it is difficult to set Köhler illumination at high magnifications. This is unconvenient but the question is what does it do to the optical performance once set up proper...

Hi Bob,
interesting, so what other turrets are you fitting to your BX?

Very nice your Othrolux - congratulatios.

Hi 75RR, do not get me wrong - I like your photos very much and I do not really care about some noise in the photos. But if you want to remove I thought it may be a good tip. Actually I do not use Photoshop anymore since I tried the DENOISE software. It allows a kind of automatisation, so several st...

It is the software DENOISE PROJECTS. It is reducing the noise but also many other kind of automized corrections.

Hi 75RR,
very nice photos.
I have filtered to remove some noise ... hope you like it.

Like it, well done!

Thanks for the info 75RR.
I often use water instead of oil for the condensor front lense, just because of ease of cleaning. Works quite well till something like n.A. 1.2 or bit more.

Very nice catches 75RR. How did you manage the oil immersion? There seems to be quite a thick liquid layer under the coverglas and this usually causes issues for such high n.a. objectives (low working distance) and oil immersion (movement or "pumping" of the coverglas due to re-focusing)? .