MicrobeHunter.com Microscopy Forum

Before you ask ... I had no idea what a ‘lutant’ was, so I went searching:
https://digitalcommons.mtu.edu/cgi/view ... ubchapters

MichaelG.

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Edit: for further explanation, see ‘luting agent’ on Wikipedia

Satinnature wrote: ↑

Thu Dec 03, 2020 10:49 pm

Would glycerol work too with regular leaves or botanical specimens ?

Not tried it myself yet, but I believe it works very well
... I know it’s recommended for pollen.

MichaelG.

Useful four-page paper available here:
https://www.researchgate.net/publicatio ... n_Glycerol

MichaelG.

Welcome aboard, Antoni

MichaelG.

Thanks for posting those, Ian The blue one https://www.flickr.com/photos/67802150@N03/50661155997/ [predictably enough] looks the sharpest, but I’m sorry to say that even there, I can see no single detail that appears in sharp focus. The stacking software will do the best it can ... but the princip...

This image is actually composed of a stack (of 8 photos), but has some nasty artefacts. This is a huge crop of the whole photograph - which in itself was taken with a X40 objective, projection lens X1.23, and optovar set to maximum. Any guidance most welcome, thank you. . To aid our ‘forensic analy...

DonSchaeffer wrote: ↑

Sat Nov 28, 2020 10:47 pm

what is wrong with this image? It looks great to me.

To put it bluntly, Don ... It has too many artefacts, and not enough real information.

MichaelG.

Looks like I may be lucky ... My Macs are too old to install Big Sur

It might be worth you looking at this discussion though:
https://developer.apple.com/forums/thread/654876

MichaelG.

This tutorial video, by Motic Europe, may help [although it demonstrates the MS Windows version] https://youtu.be/Hoev1BO_ZSg It would appear that your Swift has a smaller field of view than Motic expects ... making the 70 micron dot unusable; but you should have no problem with the linear scale. Mi...

If I recall correctly: The Motic slide also has a linear scale with 10micron divisions
... Just use that across a diameter of the field.

MichaelG.

What a strange and confused website, very pretty but I would never have known they were selling cellulose nitrate if you had not said so. Apart from "contact us" I didnt see any way of buying it. . I bought mine from ebay UK ... but many of the conservation specialists also stock it. https://www.eb...

Thanks, Bob ... that’s useful to know

MichaelG.

Cellulose Nitrate adhesive [see reference to Duco in the linked paper] is still available from HMG https://www.hmgpaint.com/news/242/hmg-paints-adhesives-tacky-products-with-a-worldwide-reputation [ gotta love their strapline :D ] It is popular with Museums and Conservators. I bought some recently, ...

Assuming that it’s the rack in your photo ... it is flexibly sprung by those slots, so alignment should not be a significant problem.

I would certainly dismount it.

MichaelG.

I would suggest thoroughly cleaning the rack before you consider replacing it.
A spot of hardened grease can cause the symptoms you describe.

MichaelG.

hans wrote: ↑

Thu Nov 26, 2020 7:31 pm

Found a schematic, figure 10 shows what the switch does:
Texas Instruments - AN-556 Introduction to Power Supplies

Thanks a lot, Hans

MichaelG.

I think you need to look for something like ‘Exposure Lock’ or ‘AE Lock’

MichaelG.

Very interesting !!

I’ve always wanted to try that, but thought it must be a complicated exercise
... He makes it look remarkably easy.

MichaelG.

Looks like the one in the PDF has the same voltage selector switch on the back as the one microb posted. . Perhaps I’m being dim, Hans ... [it wouldn’t be the first time] ... but could you please explain how voltage selection might be achieved when there are only two wires connected to the switch ?...

. I thought I had posted this last night ... but apparently not Have a look at step (3) here: https://valleymicroscope.com/wp-content/uploads/PN-10408-Olympus-BX50-Bottom-Illuminator-System-Installation-Instructions.pdf The power supply illustrated is an electronic [inverter] type, which are commonl...

mrsonchus wrote: ↑

Wed Nov 25, 2020 6:41 pm

Fixing-it now chaps!

How's that now?

Lovely ... thanks !

MichaelG.

Splendid stuff, John
... Well done, Sir

‘though I can’t currently see the images

MichaelG.

Controlling air temperature to within 0.1C is extremely demanding. Quality thermostatted ovens might reach that using a fan and thick insulated walls. . I must disagree ... Insulating materials, and control electronics, have improved greatly over recent years, and the ‘Cave’ is already stable to wi...

Beautiful results, Manfred

MichaelG.

I think temperature stabilisation would be by far the most effective approach. ... Trying to fix the problem by clever use of materials is a nice idea, but will probably set you on the road to madness. At a rough guess : you need control of air temperature to about +/- 0.1°C and the equipment stabil...

Hi folks - you won't believe this - as I was putting the slide away I dropped it then stood on it! I lost the slide - which is now in a million pieces in the bin... Sometimes these things happen I know - but I still haven't stopped muttering about it... :( You could have ‘endless hours of fun’ sear...

What is the 'std method' - are the ends of the line placed upon a punctum's central-axis for example - . It shouldn’t matter where you count from ... You are simply counting ‘cycles’ ... Just like measuring the pitch of a screw thread ... Always remember to count from Zero [ you need to count ‘fenc...

75RR wrote: ↑

Tue Nov 24, 2020 9:31 pm

.
Here is a enlargement of the first measurement which I read as 7.1µm = 14 stria
.

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Strange ... I read it as the distance between the two parallel lines being 0.71µm

MichaelG.

. It is a Gyrosigma. […] Stria are usually measured in 10µm . Making John’s approximately 14 per 10µm ... is that, in itself, sufficient for species identification ? ... I have no idea. MichaelG. On my screen the horizontal bar is 17mm, so if 17 = 70% then 100% = 24mm which gives me 18/19 stria in ...

75RR wrote: ↑

Tue Nov 24, 2020 5:42 pm

It is a Gyrosigma. […]


Stria are usually measured in 10µm

.

Making John’s approximately 14 per 10µm

... is that, in itself, sufficient for species identification ?
... I have no idea.

MichaelG.