MicrobeHunter.com Microscopy Forum

Interesting creature and very nice images!

Daphnia egg, from a cheap prepared Daphnia slide, 50x objective.

Oblique bright field, stack of 3 photos:



Dark field, stack of 2 photos:

I am not sure what kind of tree it is, but I am almost sure it is not a willow.. My photo is from a yet leafless tree. Also by "some willow" I mean "probably pussy willow". There are pussy willow photos on the net that looks quite similar to mine, like https://i0.wp.com/www.2knowandvote.com/wp-cont...

Crater Eddie wrote:No, I have never been able to see the third photo. The others are superb though.
CE

Thanks!
I've re-uploaded the image:

Thanks for the nice words!
Any help with ID? Can you even see the tree branch photo (the third one in the first post)? I sometimes can see it, sometimes can't.

Thank you for your comments!

KurtM wrote:Can you expound upon how you achieved the pseudo-SEM look?

Something like that in gimp:
- Colors -> Desaturate -> Lightness
- Colors -> Invert
- Filters -> Distorts -> Emboss -> Bumpmap with depth ~6
- adjust contrast / base curve

hkv wrote:Very nice! I wonder what the reds dots in its face are? Looks almost like nauplius eyes.

These are definitely ocelli (simple eyes).

billbillt wrote:I also love the psuedo-SEM look... If PS was not so expensive, I would have it.

If by "PS" you mean Adobe Photoshop, I'd like to let you know that, except for Zerene Stacker, I use only free/open source programs for photography: Darktable, Gimp, and ImageJ.

Pollen, stained with Lugol's iodine vapors and mounted in CytoSeal. 100x objective, oblique bright field, ~20 photos in the focus stack: https://c1.staticflickr.com/3/2864/33867690715_e6e58073dc_b.jpg Post-processing attempt to make it look like SEM (could have worked better with non-oblique bright ...

Very nice!

I've just received some Leider slides - it's one of the companies that were recommended in this thread. The set I've bought is one the Leider's "Sets of economic slides sets MICR-O-SLIDE": 59-5240 Fungi, mushrooms and algae under the microscope (Cryptogams part I). I'm really disappointed with the q...

Some look really nice!
What did you use for the post-processing?

Thanks for your comments!

Johann wrote:

kit1980 wrote:Nice. Any details how it's mounted?

Thanks Sergey. It was mounted directly in Glycerin jelly. http://www.microscopy-uk.org.uk/mag/art ... part4.html

Did you dry it? Did you put it in alcohol first?

Johann wrote:Tiny little insect from the family Thripidae.

Nice. Any details how it's mounted?

Here is a link to "meteorwrongs" .. What is not a meteorite.. From Washington University in St. Louis..... http://meteorites.wustl.edu/meteorwrongs/meteorwrongs.htm BillT Thanks for posting this link, looks interesting. Plus there are other interesting pages linked from that page, like http://meteo...

Also, many sources claims (probably copy from each other) how robust and resilient tardigrades are, specifically that they can withstand huge pressure. I'm yet to find a tardigrade to look under a microscope, but I suspect if the water evaporates, pure creature will be easily crushed by the cover gl...

I agree the image looks very suspicious. They don't claim it's alive, though. Also, pixel dimensions are suspiciously large: on http://images.sciencesource.com/preview/14006150/BS8236.html, it says the width is 13.7" at 300dpi, so it's 4110 pixels. I'm not sure there are SEMs with such pixel resolut...

charlie g wrote:I'd never have guessed these are 'oblique illuminated spores'..I'd have guessed: 'bright field' illumination!

With plain bright field the edges are much less pronounced and the whole appearance is much more flat.

Are these 'basidiomyces' type of fungus? Or are those gelatinous flaps residua of 'asci cups'..err ascomyces type of fungus? Are the spore bearing organs under those gelatinous flaps? Lastly, is lactophenol an aqueous reagent..or did you have to use alcohol with your stain? Thanks! Yes, this is a b...

Spores of this fungus are relatively large - more than 20 microns in length - and have some interesting internal structure. Mounted in lactophenol cotton blue, 100x PlanApo objective, oblique illumination, 8 photos in focus stack: https://c1.staticflickr.com/3/2915/33611916446_76910669c5_b.jpg Hue c...

I just heard about magnetotactic bacteria for the first time, and per standard operating practice Googled the subject, then turned to Youtube for the next best thing to a live demonstration. From the Wiki: "Magnetotactic bacteria (or MTB) are a polyphyletic group of bacteria discovered by Richard P...

Johann wrote:This is what I got - Assuming it is a micro meteorite

Found 3 of them fairly easily.

meteor.jpg

Nice photo!
I replied to your Facebook "mystery object" post with correct answer before seeing your post here (but I've seen previous posts in this topic before, so guessing was easy for me.)

Beautiful!

How large is it?
Very nice image!

hkv wrote:This is mine. A bit odd to find something new in box that is over 30 years old.

Do you use it? I don't remember any section photos from you...

Mushroom spore print (Mycena sp.), 2x objective, bounced light from ping-pong ball half, reflected copy added in post-processing.

I see a: 170 tube length,10X/no coverslip objective on the turrent...with it's NA below 0.4..does it matter with the coverslip on slides viewed? I see the the markings are "170/-", which means that coverslip thickness is not critical (can be used with or without coverslip). "No coverslip" would be ...

Thanks for your comments! perhaps just a subtle indication in the last picture of 'which circle comes from which part' would be useful. For this image, all circles come from the same part. I considered this for other (not yet finished) images, but it's at most two parts - top and bottom of the mushr...

Spore print, 2x objective, LED lamps trough ping-pong ball half: https://c1.staticflickr.com/1/600/33294329372_004ffd4907_b.jpg Pores, 10x objective, LED lamps trough ping-pong ball half, focus stack of ~40 images: https://c1.staticflickr.com/4/3744/33294312332_8c1ddfcc5e_b.jpg Spores 5-6 x 1.5-2 mi...