MicrobeHunter.com Microscopy Forum

mrsonchus wrote: ↑

Sun Oct 18, 2020 10:34 am

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

Hey the link doesn't work can you maybe share it again?

Our study showed that among the nine mounting methods tested, thermal inactivation of animals at 60 °C for 30 min followed by briefly pressing the coverslip with an entomological pin produced specimens fixed optimally for morphometry and imaging (i.e. maximally stretched and levelled but not deform...

Can someone help me prepare a permanent slide of a tardigrade at home the easy way? So i can observe it further

Amazing shots

How do y'all get daphnia to turn sideways. Mine just won't :/

You gather your samples similar to how I get mine. I leave the moss soaking for at least 24 hours, then give it a gentle squeeze into a watch glass and either look under a stereoscope or do a few drops of water per slide. I personally don't put a cover slip on until I see one, but stay away from th...

DonSchaeffer wrote: ↑

Mon Sep 21, 2020 1:22 am

Could be dead.

Yes i guess

Looks like a trichome to me as well

DonSchaeffer wrote: ↑

Sun Sep 20, 2020 8:12 am

that last image does look like one. Is it moving?

Waited for a day and it still didn't move :/

Sampling the next day AFTER a good shower of rain helps: gives them time to become active again. For dry moss I soak in rain water for 24 Hrs. Best, deBult Currently in India, It rains almost everyday since its the season so the moss I find is pretty much fresh. Also can Well water be used? will tr...

janvangastel wrote: ↑

Sun Sep 20, 2020 6:07 am

I think you have to soak the moss much longer. I let it soak 24 hours or even more.

Okay I will try this out too and will let you know if I'm able to find any, Thank you

Hi Shawn. The second photo you posted doesn’t look like a Tardigrade to me. How are you collecting and preparing your samples? Hello, I gather some amout of moss from a wall or a rock mostly as they're the most seen in my area and then i bring them home and soak them in bottled water in a container...

I have found something again today which looks like a tardigrade but I'm not sure about it. Need help please.

I found these two specimens in a sample from the reed beds in a local freshwater pond. One seems to have some similarities to a pair of diatoms , but unlike any I've seen before, the pair is 60um wide and 75 long, it was taken at 20x 0.46NA . The other image was taken at 40x 0.95 NA and was moving ...

Hi shawn. If you mean the wax-blocks (containing specimen for sectioning), I use those cheap plastic divided boxes that sets of screws etc come in. As far as ambient environment, room-temp is fine. The blocks really do keep well for several years at least, in my experience. I often have 'left over'...

75RR wrote: ↑

Mon Feb 10, 2020 7:33 pm

Good catch! Have not seen one myself for a while.

Here is a link to an online key: https://sun.iwu.edu/~tardisdp/keypage1.html

Very helpful for newbies like me, thank you

Hii, How do you store it once dried and cut into blocks?

Hello again...consider a nice little glass tray with plants by your microscope...flush the slide contents into this habitat...rather than 'paper wipe/ destruction' of keenly charming specimens. all the best, and again thanks for this encounter 'with a bear'...that you here forum share! charlie guev...

KD Arvidsson wrote: ↑

Fri Aug 14, 2020 1:34 pm

shawn perez wrote: ↑

Thu Aug 13, 2020 9:03 pm

What tardigrade species is this?

Hi Shawn i give you the same answer here like i did on my youtube channel. I have no idea because i am a beginner! But thanks for comment. //KD

Hey I'm sorry i did not realize its you :/

sleekdigital wrote: ↑

Thu Aug 13, 2020 10:15 pm

They are usually kind of oval shaped and usually found inside the shed skin of it's momma.

tardEggs.jpg

Thanks for sharing this image, its a lot of help

Not sure which part of the water bear are you mentioning :/

What tardigrade species is this?

Amazing

DonSchaeffer wrote: ↑

Thu Aug 13, 2020 10:21 am

I know one thing. Round-shaped things are common under the microscope. They could be cysts (or old cysts) or maybe tardigrade eggs.

Okay thank you

Observed under 10x

A few other objects of interest, although to be honest nearly everything that comes under the microscope will be new and interesting to me, but will likely have been seen many times before by the more experienced members - so apologies in advance for any Deja vu :P _MG_9638-single-frame-starblob.jp...

Micro_4129a1000.jpgI seem to have captured a heavily pregnant tardigrade. The 5 eggs in the ovary are filling the internal space rather completely! The specimen came from a sample taken in sunken leaf litter at the edge of a pond in coastal BC, under ice for the last week or so. The image was taken...

DonSchaeffer wrote: ↑

Sat Aug 08, 2020 10:02 pm

Doesn't look like one to me unless it's in a very odd position.

Okay :/

KD Arvidsson wrote: ↑

Sat Aug 08, 2020 8:58 pm

Hi Shawn! Unfortunately impossible to see in this picture for me. Do you have more pictures? //KD

This was the only picture i could get and now im unable to relocate it again :/

After long hours of searching i came across this thing which looks like an empty skin of a tardigrade but im not sure if it is an empty skin or if its a tardigrade or if its something else. Please help me out here. It consists leg like elongations and also has three round things(not sure if its insi...