Search found 273 matches

by daruosha
Mon Apr 20, 2020 9:01 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 6000

Re: Imaging with different coverglass thickness

To be honest, Andrew ... I had never heard of a #1 chambered coverslip :oops: So, I looked here for the spec. https://www.thermofisher.com/order/catalog/product/155361#/155361 ... and also saw the price I assume, and sincerely hope, that your colleagues know exactly what they are doing. MichaelG. ....
by daruosha
Mon Apr 20, 2020 8:52 pm
Forum: Microscopes and optics
Topic: Problems with Omano OM36L
Replies: 5
Views: 2361

Re: Problems with Omano OM36L

The first issue is flickering of the LED. The microscope has a wheel style brightness control and at medium to high brightness the LED flickers a lot, to the point where it sometimes goes completely dark. I have checked the batteries and they are producing the correct voltage and the flickering occ...
by daruosha
Mon Apr 20, 2020 8:39 pm
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 9080

Re: Maybe a way to get the perfect image with a DSLR?

I tried it on both 160mm (finite) and 180mm (infinity) tube length microscopes and surprisingly it was very sharp corner to corner. I don't have 3D printer at home, but I think I can design the tube in a CAD software and use the commercial 3D printing services (it's quite cheap). I have zero experie...
by daruosha
Mon Apr 20, 2020 8:34 pm
Forum: Pictures and Videos
Topic: First Photo of a Microbe
Replies: 8
Views: 4186

Re: First Photo of a Microbe

Nice Picture Roger. It's a Daphnia IIRC.
by daruosha
Mon Apr 20, 2020 8:08 pm
Forum: Camera systems and imaging
Topic: Olympus DP25
Replies: 6
Views: 3229

Re: Olympus DP25

Well, if you have a USB 3.0 on your laptop, you can use an adapter like this on to "adapt" the connection, not the connection protocol itself. I cannot guarantee a successful connection, but it's not a money loss I guess. Worth a try. https://www.digikey.ca/product-detail/en/qualtek/3021019-16/Q370-...
by daruosha
Mon Apr 20, 2020 7:56 pm
Forum: Camera systems and imaging
Topic: Olympus DP25
Replies: 6
Views: 3229

Re: Olympus DP25

Does it have thunderbolt port? What's the exact model of the laptop?
by daruosha
Mon Apr 20, 2020 7:49 pm
Forum: Camera systems and imaging
Topic: Olympus DP25
Replies: 6
Views: 3229

Re: Olympus DP25

Kirby,

what type of machine do you have? Mac? PC? Laptop or desktop? What's the operating system?
by daruosha
Mon Apr 20, 2020 7:46 pm
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 9080

Re: Maybe a way to get the perfect image with a DSLR?

On a more serious note we currently know using an extender is working for Malpi and me... and "just the two of us" is not enough to be sure it can be a solution for most users. So if you can test this on your microscope and let us know what's the results, it will be appreciated. Mate, I tried Canon...
by daruosha
Mon Apr 20, 2020 7:09 pm
Forum: Camera systems and imaging
Topic: Help identifying this mount
Replies: 3
Views: 2137

Re: Help identifying this mount

The tube is exactly like the one came with my stereo microscope, but it was to wobbly and I found a reduction lens with the same dovetail mount so I replaced the tube with the reduction lens. https://ae01.alicdn.com/kf/HTB1uSEXXwsSMeJjSspeq6y77VXat.jpg I saw many other reduction optics (2x and 1.5x ...
by daruosha
Mon Apr 20, 2020 6:50 pm
Forum: Pictures and Videos
Topic: Cytoplasmic streaming (cyclosis) in onion cells
Replies: 8
Views: 3030

Re: Cytoplasmic streaming (cyclosis) in onion cells

Impressive footage Wes. I had seen this activity (Cytoplasmic streaming is bit mouthful for an electronics guy like me :D) on one of Oliver's video. But you clearly nailed it.
by daruosha
Mon Apr 20, 2020 6:29 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 6068

Re: More phase contrast photos of cleaned diatoms

@daruosha: Here is an article about cleaning diatoms with hypochlorite: A Simple, Inexpensive Method for Cleaning Diatoms Author(s): Jennifer M. Carr, Gary L. Hergenrader, Nels H. Troelstrup and Jr. Source: Transactions of the American Microscopical Society, Vol. 105, No. 2 (Apr., 1986),pp. 152-157...
by daruosha
Mon Apr 20, 2020 6:22 pm
Forum: Identification help
Topic: UFO Unidentified object!
Replies: 8
Views: 5207

Re: UFO Unidentified object!

Interesting video as always KD. You did some actual microbe hunting :)
by daruosha
Mon Apr 20, 2020 6:19 pm
Forum: Beginner's corner
Topic: Imaging with different coverglass thickness
Replies: 18
Views: 6000

Re: Imaging with different coverglass thickness

Hi, My name is Andrew, I'm new to the forum. I have a question about why a lab in my institution is using a #1.0 chambered coverslip for an objective lens that's meant for 0.17 thickness coverslip. I have tried using #1.5 chambered coverslip but the image never focuses. The lab does calcium signali...
by daruosha
Mon Apr 20, 2020 6:11 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 6068

Re: More phase contrast photos of cleaned diatoms

Thanks, Bob and darousha. @darousha: Diatoms were cleaned with APS - see this post: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=10&t=8101&p=72044&hilit=APS#p72044 One step of boiling in APS, then, instead of repeating it, I rinsed with distilled water, then applied house-grade hyp...
by daruosha
Mon Apr 20, 2020 6:06 pm
Forum: Illumination Techniques
Topic: PDFs of the VAC articles/papers?
Replies: 2
Views: 2215

Re: PDFs of the VAC articles/papers?

I'm interested too. Would you post the PDFs here in this public thread? (In case its not a copyright infringement issue)
by daruosha
Mon Apr 20, 2020 3:07 pm
Forum: Specimens, samples and slides
Topic: More phase contrast photos of cleaned diatoms
Replies: 18
Views: 6068

Re: More phase contrast photos of cleaned diatoms

Hobbyst46, they are beautiful and stunning.

May i ask how do you clean your diatoms? (Sorry for an irrelevant question).
by daruosha
Mon Apr 20, 2020 2:59 pm
Forum: Introduce yourself
Topic: Hello everyone.
Replies: 6
Views: 3238

Re: Hello everyone.

Hello Zohar, welcome aboard.

Based on your introduction, I assume you're interested in botanic sectioning and staining techniques. Check out the link below and you will find awesome stuff :
http://www.mikroskopie-bonn.de/index.php
by daruosha
Mon Apr 20, 2020 2:55 pm
Forum: My microscope
Topic: USB phone setup hacked together with 3D printer and Zeiss Base.
Replies: 3
Views: 2281

Re: USB phone setup hacked together with 3D printer and Zeiss Base.

Awesome and very creative restoration project. Well done.
by daruosha
Mon Apr 20, 2020 11:07 am
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 17
Views: 4970

Re: Stomata in tulip leaf

The first image is very eye catching to me. Nice tips/techniques too.
by daruosha
Sun Apr 19, 2020 10:32 am
Forum: Camera systems and imaging
Topic: Maybe a way to get the perfect image with a DSLR?
Replies: 26
Views: 9080

Re: Maybe a way to get the perfect image with a DSLR?

Can you show us a picture of the actual setup and a sample picture?
by daruosha
Sun Apr 19, 2020 10:11 am
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 17
Views: 4970

Re: Stomata in tulip leaf

Nice job Bob. I have a few tubes of UV curable glue for repairing PCBs. I'll like to try your recipe and see how it works. The glue I have is not fully transparent, but I think it doesn't matter.
by daruosha
Sun Apr 19, 2020 9:53 am
Forum: Pictures and Videos
Topic: spring'2020 bear hunt with questions'.
Replies: 27
Views: 10856

Re: spring'2020 bear hunt with questions'.

charlie g wrote:
Sat Apr 18, 2020 8:04 pm
Water bears do not have relativly large loose cells..freely jiggling within their bodiy cavities...especially within their hear region.
The best explanation : ) Especially the first picture. LOL.
by daruosha
Sat Apr 18, 2020 6:13 pm
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 17
Views: 4970

Re: Stomata in tulip leaf

That's very cool Bob. Any reason why did you use UV curing nail polish? To make it dry faster, I assume. Am i right?
by daruosha
Sat Apr 18, 2020 10:56 am
Forum: Introduce yourself
Topic: Hello from Hong Kong
Replies: 3
Views: 2551

Re: Hello from Hong Kong

Welcome to the forum Sir.
by daruosha
Sat Apr 18, 2020 10:54 am
Forum: Beginner's corner
Topic: From New Zealand & new to microscopy
Replies: 6
Views: 2763

Re: From New Zealand & new to microscopy

Welcome to forum Saby. Nice microscope, you have :)
It's very useful to have a trinocular head on your microscope. It surprise me way before this digital era and the availability of cheap digital cameras, any half decent and above microscope has been equiped with a trinocular head.
by daruosha
Sat Apr 18, 2020 10:41 am
Forum: Microscopes and optics
Topic: Working distance
Replies: 4
Views: 2498

Re: Working distance

Ironically the typical working distance of a generic 40x NA0.65 achromatic is about 0.65mm.
by daruosha
Sat Apr 18, 2020 10:30 am
Forum: Miscellaneous
Topic: Slides
Replies: 5
Views: 3150

Re: Slides

Somewhere in the forum i read the optimum slide thickness for the best resolution is 1mm.
by daruosha
Fri Apr 17, 2020 5:32 pm
Forum: Microscopes and optics
Topic: Highest power LED suitable for microscope?
Replies: 15
Views: 6301

Re: Highest power LED suitable for microscope?

PeteM wrote:
Fri Apr 17, 2020 5:12 pm
Not clear to me if the dome on many of these LEDS is a plus (brightness) or minus (uneven illumination).
If you can, get a LED without the dome assembly. The dome acts like a lens and disturbs the microscope lighting optics arrangement.
by daruosha
Fri Apr 17, 2020 1:59 pm
Forum: Microscopes and optics
Topic: Retrodiode LED conversion
Replies: 15
Views: 6005

Re: Retrodiode LED conversion

I checked their website. My educated guess tels me the heat sinks are 3-5W rated.
by daruosha
Fri Apr 17, 2020 11:55 am
Forum: Microscopes and optics
Topic: Highest power LED suitable for microscope?
Replies: 15
Views: 6301

Re: Highest power LED suitable for microscope?

DrPhoxinus wrote:
Fri Apr 17, 2020 11:48 am
Saul,

Can you tell me what a Type 2a power supply is?

I looked on the web and haven’t found a description.

I have been fooling around and ran into Ohm’s law with the current limits and voltage drop in small conductors.

Gerard
He meant 2 amps capable power supply.