MicrobeHunter.com Microscopy Forum

In my experience, closing the field iris a bit and using bigger patch stop disks yields a more DOF in the final image. Adjusting condenser height also helps better contrast. In case of your microscope, make hole on a cardboard and put in on top of the light assembly. Try different hole sizes and use...

Crisp and well illuminated. Nice video KD.

I suggest try different size dark field patch stop disks for optimum depth of field and background darkness. I guess the last microorganism in the video is a Paramecium.

Congrats for yet another beautiful footage.

Excellent tips Bob. Tomorrow I'll do another run and try my best to document my process. Hopefully the result will be much better. I was rather successfully in the past when it comes to staining botanic cross sections. My biggest problem is that I don't follow my exact protocol (I like to take short...

shirleey22 wrote: ↑

Thu Apr 16, 2020 1:16 pm

it looks amazing honestly! <3

Thanks for your nice comments shirleey22. I assume you have similar swift microscope. Try some specimens and show us your pictures.

It's a very nice looking beast. You attached two analogue/film camera at both side of the microscope. Do I see and recognize them correctly? What's their purpose when you have a another digital camera on top of the ocular?

I'd like to recommend the following article which answers a lot of questions about Russian microscope industry:

Why are Russian optics so good? http://www.novacon.com.br/lenses08.htm

The objective lens you linked in the first post has the NA 0.65 which is in the range of achromatic objectives. I though apochromatic objectives have higher NA. Is my assumption correct?

I don't know the exact model of your microscope, but I'm sure @apochronaut knows a lot of American Optics models and will help you later on.

Charlie g, I cannot answer your last question, but have to say I enjoyed reading your bear hunting story. Thumbs up.

I can never get them neat! DonSchaeffer , yes you definitely can do it. It's nothing hard or difficult, it's time consuming and all you need is passion. As I mentioned earlier, I'm writing a guide about sample preparation and will start a separate topic. If you can read the German language: the mic...

KD Arvidsson wrote: ↑

Wed Apr 15, 2020 9:50 pm

Great pictures Daruosh! Thanks //KD

Thank you KD.

shirleey22 wrote: ↑

Thu Apr 16, 2020 12:57 am

which microscope was used?

It's a cheap Chinese no name microscope. I've made a few modification. but it's nothing fancy or special at all.

A good quality 10 Watt led (e.g. Cree) will suffice for most dark field, DIC and phase-contrast situations when taking pictures. For observation only even 10 Watt is overkill in most configurations. My personal experience only, your milage may vary. %100 agreed. I have retrofited my microscope with...

Welcome to the forum. Do you have any background in medical science? What kind of analysis so you want to do? Amateur microscopy is much more than observing blood cell (unless you're doing it professionally). Just as you mentioned, taking pictures of microorganisms is a lot more amusing and rewardin...

KD Arvidsson wrote: ↑

Wed Apr 15, 2020 9:36 pm

Yes i live about 1 km from the sea on the westcoast of Sweden so i have much water from salt water to water from lakes and ponds Etc...

Yeah, I can imagine. Sweden is a heavily taxed heaven : ))

It's crazy. 1500W ! I cannot imagine any microscopy scenario requires a 1500W LED module. Can you?
Remember, it emits more than 100,000 lumens!

What a beast. Looks gorgeous too.

ThomasMc wrote: ↑

Wed Apr 15, 2020 8:27 pm

It's really heavy, but not actually causing the table top to bend.

I can see the bend on the table top surface

Your videos are getting better and better.

Thanks for sharing it KD.

There are several microscopy mountants that you can choose based on what are you trying to mount on the slide. A very basic and easy to start mountant is Nail Polish. It's not perfect, but you have to try it out and find how it works for you. For mroe details about NPM, check out this artilce from M...

Welcome to the forum Gslab. I just bought a very old AO Spencer "Black Beauty" scope from 1950's. Having a lot of fun with it, and would love to learn more about how to use it and improve its performance. I'me sure you'll find everything you need in the forum. It's a very rich and addictive environm...

In general, I would avoid xylene. It is a suspected carcinogen. You're absolutely right. I know HistoClear is a very safe alternative for Xyline and it's compatible with all Xyline based mountants. Anyone have any experience with HistoClear? Unfortunately its not available here in my country, so I ...

Hi Daroush, does your dimmer module offer a continuous current with no ripple or flickering? I started my LED experiments with a PWM board and it gave me trouble with striped photos. Bob Hi Bob, LTC3780 chip is a buck-boost switching regulator controller. But due to an internal PLL, it can lock up ...

-How can I peel off a coverslip that has stuck to the slide? Totally Deepens on the mountant, in case of water, put in a warm bath of water and let the water soak beneath the coverslip and gently slide the cover slip to the edge of the slide. For more specific answers, you need to specify the mount...

The LED module which you've ordered is actually an array of 3 parallel groups of 3 LEDs in series (9 LED in total), hence this particular arrangement the working voltage of the LED module is 3 times of each LED forward voltage (9-12V DC). However unknown/no-name brands tend to be much less efficient...

Hi dzarren, Here's my simple yet very reliable recipe: LED: Cree XML2 (10W) 10W is a bit overkill in bright field, but dark field and oblique illumination is where a 10W LED really shines. You can choose much faster shutter speeds in bright field as well. Highly recommended. If you can, get XML2 ver...

Hi dzarren.

First of all, welcome to the forum. It's certainly a beautiful microscope. I recommend a new LED retrofit as well.

for interpreting the specs and writings on objectives, I suggest the following article:
https://www.microscopyu.com/microscopy- ... ifications

Wes, the footage is beautiful and nicely done. I have a 50D as well and I use it solely for microscopy. I was aware of MagicLantern for years but never knew how it would perform and affect the actual usability of the camera(bugs, etc..). Your video made me more curious and I think I'll give MagicLan...

Thanks for sharing a very interesting lab exercise. I encourage other members to read the paper too.

Beautiful sections. I would be interested in a rundown of your microtechniques. Thanks BramHuntingNematodes for your kind comments. I'm in the process of writing a rather comprehensive document about my sample preparation and a survey of all the techniques I've learned so far. Will start a serial t...

It appears as if your stains have mixed and formed a new colour instead of attatching to different structures. Have you tried the stains alone and have you tried to differntiate them by removing part of the staining so lightly stained structures are destained? Bob I found the exact same problem dur...

Thanks Bob and BramHuntingNematodes. I'm sorry that I didn't explain anything at all. I did all the cutting, staining, mounting and photography in one single session (nearly 5 hours) and lost my energy at the very last minute of posting the pictures. The first image is a cross section of pathos leaf...