Search found 1854 matches
- Sun Feb 07, 2016 4:38 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
I wish I could share the actual 'raw size' image captures of the Heliozoan. Shame on me,,I was in a hurry looking for bears...I did not bother to use the higher power objectives on this graceful protist..such aplomb as at times nanoflagelates came near it. charlie guevara who need to get his err sea...
- Sun Feb 07, 2016 4:30 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
yes DF looks better than I image capture it...the cillia on that protist were beating metachronally...twikling 'peach fuzz'!
- Sun Feb 07, 2016 4:20 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
Yes these Heliozoas are gorgeous...and as you aptly noted in another thread,Fan...'dark field back ground is jet black'!
- Sun Feb 07, 2016 4:18 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
Ooop,s Fan, my bad!
- Sun Feb 07, 2016 4:15 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
Hi charlie g, what exactly are we looking at here? Hi...murphys law!! you posted while I am putting up 10X and 20X and 40X DF images from an old (weeks old) moss sample. I was hopeing to encounter a few more bears. When you let the moss sample soak a day..I still consider it fresh sample. Every bea...
- Sun Feb 07, 2016 4:05 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Re: Dark Field contrast during a hunt.
We collected tree base mosses just as we had on our succesful bear hunt (tardigrades) a few weeks ago. The samples are now at my bench with a drizzle of water...I plan to observe for bears before the Super Bowl. Before I transfered the moss sample from the last bear hunt, I tried a DF scan of a slid...
- Sun Feb 07, 2016 3:52 pm
- Forum: Pictures and Videos
- Topic: Dark Field contrast during a hunt.
- Replies: 11
- Views: 2430
Dark Field contrast during a hunt.
Hi all...yes it was in balmy 40's during the day for almost a week, so good doggies and I went on a woodland hunt for bears(tardigrades).
- Sun Feb 07, 2016 3:29 pm
- Forum: Pictures and Videos
- Topic: COL vs. Brightfield
- Replies: 20
- Views: 4490
Re: COL vs. Brightfield
I look forward to your trials with this COL contrast method,gekko. I now see (pun) that when all illumination is from beneath the specimen..it's all: 'conscopic illumination'? When illumination comes from above the specimen...it's 'episcopic illumination'. So you can have oblique illumination by my ...
- Sun Feb 07, 2016 3:17 pm
- Forum: Introduce yourself
- Topic: Hi; I'm Duncan
- Replies: 12
- Views: 5606
Re: Hi; I'm Duncan
Welcome,Duncan! charlie guevara, finger lakes/US
- Sun Feb 07, 2016 3:13 pm
- Forum: Miscellaneous
- Topic: Variety of microbes?
- Replies: 8
- Views: 4604
Re: Variety of microbes?
Three (3) charlies in a row...this thread is chuck full! charlie guevara
- Sun Feb 07, 2016 3:10 pm
- Forum: Miscellaneous
- Topic: Variety of microbes?
- Replies: 8
- Views: 4604
Re: Variety of microbes?
Hi...land,sea,air...we need to think of communities sorted out with:'microhabitats'...or nitches (?sp?). Though many organisms 'seem' ubiquitous...there are predictable 'micro habitats' within which one expects groupings of organisms. So dropping down the rabit hole to microbes...these sort out in g...
- Sun Feb 07, 2016 2:58 pm
- Forum: Introduce yourself
- Topic: Greetings
- Replies: 12
- Views: 5657
Re: Greetings
Welcome,Ray! Err...panthers or bronco's? charlie guevara,finger lakes/US
- Sat Feb 06, 2016 6:48 pm
- Forum: Pictures and Videos
- Topic: COL vs. Brightfield
- Replies: 20
- Views: 4490
Re: COL vs. Brightfield
Hi all. Say gekko...is it that DF is a specific and crisp optical plane of a condensers illumination cone hitting objects? And is COL a softer range of the condensers illumination cone hitting objects, rather than COL occurring at a crisp optical plane? When I mix my Labophot condensers phase discs ...
- Sat Feb 06, 2016 6:01 pm
- Forum: Pictures and Videos
- Topic: Diatom under polarized light
- Replies: 14
- Views: 3141
Re: Diatom under polarized light
Beautiful,gekko! Is that jet black background 'image processed'..or do your pol filters when CX/POL give that perfect extinction?!
Are your pol filters DIY, or are they a microscope firm set of pol filters, gekko?
Quite an image, love the green glows. charlie guevara
Are your pol filters DIY, or are they a microscope firm set of pol filters, gekko?
Quite an image, love the green glows. charlie guevara
- Sat Feb 06, 2016 5:50 pm
- Forum: Camera systems and imaging
- Topic: Anything else I need?
- Replies: 3
- Views: 3191
Re: Anything else I need?
Hi...I am not an expert...but: that photo tube has the specific specs on it for use wither the 12X ocular nested inside it... or the 10Xocular (plus a 5 mm spcer ring to have it parfocal with the 12Xocular). So it performs as a camera adapter with a nested occular. Folks with more knowledge on this ...
- Sat Feb 06, 2016 4:37 pm
- Forum: Pictures and Videos
- Topic: Ciliate collapse
- Replies: 13
- Views: 3988
Re: Ciliate collapse
Good video clip...but sad demise...I sense it was a gentle cover slip crunch from objective..as the objective 'seeks focus'...right at the git-go there is out of focus first damage of the cell body...and that spinning easily could be osmotic fluid currents as this bleak punctured protist 'disembowls...
- Sat Feb 06, 2016 4:05 pm
- Forum: Pictures and Videos
- Topic: Basic (very) polarizer tested
- Replies: 5
- Views: 1875
Re: Basic (very) polarizer tested
Great work,John B...and JimT! Please consider the low cost 'Lunar polarizer filters'...very quality filters, low cost...already paired together in metal mount rings...yet they come apart so you have your polarizer and analyzer filters to place at the different locations in your light train. any onli...
- Sat Feb 06, 2016 2:30 am
- Forum: Pictures and Videos
- Topic: Pond Porcupine
- Replies: 26
- Views: 7082
Re: Pond Porcupine
The magnification listed is the objective magnification. 100X was 100X oil immersion. The DF images were taken straight to sensor from the objective with just the telon lens in the head as an intermediary. Diastar 420 microscope. The one marked 10X( the last one) was with a 100X phase annulus and a...
- Fri Feb 05, 2016 2:24 am
- Forum: Pictures and Videos
- Topic: Pond Porcupine
- Replies: 26
- Views: 7082
Re: Pond Porcupine
Love your 'porcupine' ,Phil. Years ago I chased one down a rural shale road with a huge landing fish net.( a mamalian porcupine in Chenango Co,/NY)..then as it lumbered ahead of me I thought...naw...hard to pet. But when our son was a child...we enjoyed for years, european hedge hogs as dear pets! S...
- Wed Feb 03, 2016 11:39 pm
- Forum: Pictures and Videos
- Topic: Phacus video in darkfield
- Replies: 20
- Views: 5162
Re: Phacus in darkfield
I experience a totally similar 'glare obstruction' in my crude attempts to image capture simple 'CX/Pol' images of jemstone inclusions of stream bed clays...visually the color patterns are stunning...but for my image captures...oh so many colors are 'drowned out' by brilliance of the microcrystals o...
- Wed Feb 03, 2016 11:27 pm
- Forum: Pictures and Videos
- Topic: Phacus video in darkfield
- Replies: 20
- Views: 5162
Re: Phacus in darkfield
Congrats, zzffnn, on an enchanting image to my eyes. What you imaged captured is the pellicle of a deceased Phacus species. This pellicle is quite diagnostic for type of flagellate..just as the crystaline jewel-box frustules of dead diatoms are diagnostic. This pellicle has value to many...just as t...
- Wed Feb 03, 2016 9:31 pm
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Thank you, zzffnn, and gecko! My Labophot turrent condenser is:1.25...it is that DF turrent setting which gave that image, ah..as gecko advises..I should go back and look at this diatom slide and take note of the background color visually...and then compare to the image capture background color. But...
- Wed Feb 03, 2016 1:24 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
To zzffnn's comments, I would guess that using the Plezy, even if it doesn't give you the exact tube length correction, will give a better approximation than an optic-less adapter (just a guess). Charlie, for what it is worth, I assume that your condenser's NA is greater than one, then yes, you can...
- Wed Feb 03, 2016 1:16 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Regarding what immersion for condenser: In some cases, you need oil instead of water. For example, if you are doing darkfield or oblique with a condenser with maximum NA of 1.4 or above. If you are using an oil Abbé condenser with NA 1.25 for brightfield, then oil would not do much more than water ...
- Wed Feb 03, 2016 1:07 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Charlie, I heard Leitz Plezy was designed for adapting 37mm parfocal objectives to 45mm parfocal objectives. Some did use Plezy to adapt LOMO WIs to DIN scope and considered it fine. Optically though, it is not an exact fit. I would guess (though I could be totally wrong) that tube length addition ...
- Wed Feb 03, 2016 12:57 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
So with these two coupled adaptors...my: LOMO BF 70X apo, and my LOMO DF and Phase 70X apo work on my Nikon Labophot stand. I have not yet ciphered how to achieve the 'phase contrast' contrast method with this spec'ed objective. Again....1) what type of water for the objective bridge? Fan is a fan o...
- Wed Feb 03, 2016 12:38 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Thanks for the comments...clearly in forum when I 'whined' how I jumped at purchase of three LOMO apo's as the cost was so reasonable...forum opine in 2010 was: 'Plezy adaptors will permit these for use in a DIN stand'...dugghh...so I could not locate Plezy adaptors until 2015 ! charlie guevara
- Wed Feb 03, 2016 12:31 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Thanks for the thoughts...now...do I 'water bridge' between condenser and slide undersurface to achieve the LOMO spec of NA 1.23? As oil bridge is more 'controlable'...would oil-bridge between condenser and slide undersurface affect performance of these low cost LOMO apo's...with water immersion of ...
- Wed Feb 03, 2016 12:18 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Thanks for any commets...what are thes two silver metal adaptors? Quit a few years ago in great microscopy forum: "Microscope"...I was advised that the: Plezy adaptor alone would permit these RMS 'short barrel objectives ' to function on my DIN (45 mm spec objective lens barrel Nikon Labophot stand....
- Wed Feb 03, 2016 12:06 am
- Forum: Microscopes and optics
- Topic: LOMO apo WI objectives on my DIN stand, please advise ?!
- Replies: 20
- Views: 7253
Re: LOMO apo WI objectives on my DIN stand, please advise ?!
Hi, of course I hand held each of these pleasant silver metal adaptors over fine print on paper...no obvious 'lens affects' noted in the view of fine print through these adaptors...thanks for your comments...thanks for you forum sharing. charlie guevara