Search found 124 matches
- Thu Apr 16, 2020 3:07 am
- Forum: Pictures and Videos
- Topic: Paramecium in a different light
- Replies: 16
- Views: 6165
Re: Paramecium in a different light
Re intracellular crystas in Paramecium - nice pictures. I have also photographed them and made movies of their birefringence under polarized light. I was trying to find out more about them and found a link to this discussion. I had read some of the references already mentioned and also that the crys...
- Tue Apr 14, 2020 3:39 am
- Forum: Pictures and Videos
- Topic: Stigonema Mammilosum
- Replies: 8
- Views: 3520
Re: Stigonema Mammilosum
Beautiful photomicrographs.
RB
RB
- Fri Apr 10, 2020 11:58 pm
- Forum: Pictures and Videos
- Topic: Fluorescent Microorganisms
- Replies: 3
- Views: 1995
Fluorescent Microorganisms
Photos taken with a Nikon Optiphot wide field fluorescent microscope using 10, 20X Fluor objectives, and UV excitation. Samples are from my fresh water aquarium. I used a Nikon DSLR camera. Specimens stained with 1 microgram\ml Acridine orange. most specimens survive in this stain for more then 6 ho...
- Fri Apr 10, 2020 11:41 pm
- Forum: Pictures and Videos
- Topic: Pine needle-fluorescence,
- Replies: 11
- Views: 4405
Re: Pine needle-fluorescence,
Very nice images of the plant sections.
Cheers
RB
Cheers
RB
- Wed Apr 01, 2020 4:26 am
- Forum: Pictures and Videos
- Topic: Freshwater copepods and Stentor
- Replies: 2
- Views: 1312
Freshwater copepods and Stentor
I am attaching a few copepods photomicrographs using a variety of different microscopy techniques.
All taken at 50X magnification. Organisms from my fresh water aquarium I keep for winter observation.
All taken at 50X magnification. Organisms from my fresh water aquarium I keep for winter observation.
- Sun Mar 29, 2020 8:28 pm
- Forum: Pictures and Videos
- Topic: Cladocerans and a Copepod various techniques
- Replies: 0
- Views: 1306
Cladocerans and a Copepod various techniques
Including some pictures of a Cladoceran (Chydoridae) and a copepod - I am working on the species identification (Alona sp?) for the Chydoridae member. I used an old Nikon Optiphot for the Fluorescence, Axioscope for Rheinberg and Polarization microscopy. Just waiting for local ponds to open up so I ...
- Tue Mar 17, 2020 4:06 am
- Forum: Pictures and Videos
- Topic: Dileptid Hunting and Eating
- Replies: 17
- Views: 9906
Re: Dileptid Hunting and Eating
Nice movie Bruce - wouldn't it be nice to be able to just zoom in on the organism rather that have to change objectives.
The Darkfield is nice and the ciliates appears to glow.
Cheers
RB
The Darkfield is nice and the ciliates appears to glow.
Cheers
RB
- Tue Mar 10, 2020 8:46 pm
- Forum: Pictures and Videos
- Topic: Rotifer article with pictures and video
- Replies: 3
- Views: 4182
Rotifer article with pictures and video
Fellow microscopists - I just posted an article about Rotifers mostly Branchionus and Philodina, but there are pictures of other rotifers. I included a movie of these rotifers using a variety of microscopy techniques including: Phase contrast, DIC, Rheinberg, Darkfield, Bright field and Fluorescence...
- Mon Mar 02, 2020 12:31 am
- Forum: Pictures and Videos
- Topic: Rotifers - Brachionus manjavacas and Philodina roseola
- Replies: 5
- Views: 3407
Rotifers - Brachionus manjavacas and Philodina roseola
Dr. Terry Snell a rotifer biologist in the US sent me some Brachionus manjavacas and Philodina roseola species of rotifers to take photos of for social media. He provides rotifers for aquaculture and for toxicology testing. I used a variety of microscopy techniques, Bright field, DIC, Phase contrast...
- Mon Mar 02, 2020 12:05 am
- Forum: Pictures and Videos
- Topic: Spirostomum teres
- Replies: 4
- Views: 3086
Re: Spirostomum teres
Very nice DIC images of these ciliates.
RB
RB
- Mon Mar 02, 2020 12:04 am
- Forum: Pictures and Videos
- Topic: More living diatoms II
- Replies: 7
- Views: 4240
Re: More living diatoms II
Nice photos of Live Diatoms.
Cheers
RB
Cheers
RB
- Wed Jan 22, 2020 4:39 pm
- Forum: Pictures and Videos
- Topic: The Microscopic Beauty of Plants & Tree
- Replies: 9
- Views: 3599
Re: The Microscopic Beauty of Plants & Tree
In answer to some questions. The branch was cut fresh, clamped and sectioned as is no fixing or softening as I did in the past. Softening the tissue by boiling in 10% glycerol and water seems to cause the bark to separate. The microtome cost me $300 US on Ebay, but I have never seen another one like...
- Wed Jan 22, 2020 5:43 am
- Forum: Pictures and Videos
- Topic: The Microscopic Beauty of Plants & Tree
- Replies: 9
- Views: 3599
Re: The Microscopic Beauty of Plants & Tree
Hi Thanks the web site is amazing and has beautiful photomicrographs. Today I tried an older AO Hand microtome from 1950s I purchased on Ebay and a new disposable microtome blade from India and right away I was able to cut sections between 10-15 microns - I might not need to buy a sliding microtome ...
- Tue Jan 21, 2020 1:55 am
- Forum: Pictures and Videos
- Topic: The Microscopic Beauty of Plants & Tree
- Replies: 9
- Views: 3599
Re: The Microscopic Beauty of Plants & Tree
Thanks for the suggestions I had a look at the device you made out of plastic I will try something similar. You say you make 7- mcron sections - I would like to make 1015 micron sections so I am looking at purchasing a sliding microtome. You have some very nice sections - I still have a lot to learn...
- Mon Jan 20, 2020 5:56 am
- Forum: Illumination Techniques
- Topic: Bertrand Lens
- Replies: 1
- Views: 5045
Re: Bertrand Lens
I have a Bertrand lens in my Unitron Polarizing microscope. To use it I need to use the 40X objective- doesn't work with the other powers. The easiest sample for testing is to put a thin slice of mica where the slide would go. You may have to remove the eye piece to see the interference pattern and ...
- Mon Jan 20, 2020 5:39 am
- Forum: Illumination Techniques
- Topic: dIY DIC
- Replies: 68
- Views: 38850
Re: dIY DIC
I have DIC, Hoffman and I used oblique illumination which the article refers to. I believe the author has now purchased DIC. DIC is unfortanetly very expensive to make. A number of researchers have been working on developing software that is supposed to simulate DIC or Phase contrast, but none has a...
- Mon Jan 20, 2020 5:27 am
- Forum: Illumination Techniques
- Topic: Rheinberg Filters
- Replies: 8
- Views: 8982
Re: Rheinberg Filters
The Rheinberg filters on Ebay are excellent I have purchased and used them on several of my microscopes. They cost about $30 which is cheap in my opinion. You do need to adjust the height of the filters under the condenser for different objectives. What I did with my research microscope is create a ...
- Mon Jan 20, 2020 5:18 am
- Forum: Illumination Techniques
- Topic: why are my photo's and video's always yellowish?
- Replies: 11
- Views: 6399
Re: why are my photo's and video's always yellowish?
Re yellow photos - you can do one of four things to fix the background colour: 1) use a blue filter - they are sometimes called daylight filters, and use the max brightness of your bulbs 2) You can set you camera white balance to tungsten about 3200 K 3) You can fix the colour quickly if you use Pho...
- Mon Jan 20, 2020 5:10 am
- Forum: Digital processing
- Topic: Stacking Software
- Replies: 47
- Views: 48256
Re: Stacking Software
HI I tested all three programs I use Photoshop regularly, Helicon was better and both support RAW files. Zerene was Ok, but did not support RAW images. https://www.canadiannaturephotographer. ... cking.html
- Mon Jan 20, 2020 5:06 am
- Forum: Digital processing
- Topic: Digitizing 35mm micro photography tranparency images
- Replies: 11
- Views: 12082
Re: Digitizing 35mm micro photography tranparency images
Hi - I have digitized lots of slides for clients using a Nikon coolscan and the Epson V600. The coolsan offers the highest quality, but a full res scan can take 5 minutes. The Epson will scan up to 6 images simulataneously and does a very good job, not as good as the Cools scan but good enough for m...
- Mon Jan 20, 2020 4:52 am
- Forum: Digital processing
- Topic: Photoshop CS6 v Helicon v Zerene,and the rest
- Replies: 22
- Views: 15972
Re: Photoshop CS6 v Helicon v Zerene,and the rest
Hi I focus stack images regularly and compared Photoshop, Helicon and Zerene and posted my result on my web site. I use Photoshop for quick testing and it is often all I need. Helicon is better and offers more features for blending. I work with RAW files which Zerene did not support at time I did my...
- Mon Jan 20, 2020 4:33 am
- Forum: Pictures and Videos
- Topic: The Microscopic Beauty of Plants & Tree
- Replies: 9
- Views: 3599
The Microscopic Beauty of Plants & Tree
I just posted an article on my web site that I think some members might be interested in called the The Microscopic Beauty of Plants and Trees. I have been hand sectioning plants and trees and tried a wide variety of disposable razor blades, shaving blades and microtome blades. I am using hand micro...
- Mon Sep 02, 2019 8:36 pm
- Forum: Pictures and Videos
- Topic: Micro-Universe
- Replies: 4
- Views: 2486
Micro-Universe
Can't believe summer is over and I only had a few opportunities to photograph my favourite subject - Pond Life. I just posted two articles 1) The Micro-Universe and 2) Microscopic Pond Life for those that might be interested: 1. https://www.canadiannaturephotographer.com/microuniverse.html 2. https:...
- Mon May 13, 2019 2:39 am
- Forum: Pictures and Videos
- Topic: Micro-Universe Presentation
- Replies: 4
- Views: 3443
Micro-Universe Presentation
I am giving a presentation at a public library to try and get more folks interested in microscopy, I am attaching a couple of the images I am going show in the presentation. I also have a series of movies to show pond organisms, ciliates, rotifers, amoeba etc. I also have a picture that kids took wi...
- Sun Apr 21, 2019 10:40 pm
- Forum: Pictures and Videos
- Topic: Ascorbic Acid eyes
- Replies: 4
- Views: 5204
Re: Ascorbic Acid eyes
Definintely Vitamin C - I see lots of similar crystals like this - very beautiful.
RB
RB
- Mon Apr 15, 2019 3:26 am
- Forum: Pictures and Videos
- Topic: Tips on Buying a Microscope and suggestions for What to look at
- Replies: 16
- Views: 9979
Re: Tips on Buying a Microscope and suggestions for What to look at
The LEDs I got were 20W Cree from Stanton on Ebay, not exactly sure what the colour temperature is - looks like about 6500K, but unlike tungsten which are warm 3200-3400 K, these LEDs seem almost blue in colour. I still colour correct the background so its a neutral white using RGB numbers in Photos...
- Sun Apr 14, 2019 3:29 am
- Forum: Pictures and Videos
- Topic: Tips on Buying a Microscope and suggestions for What to look at
- Replies: 16
- Views: 9979
Re: Tips on Buying a Microscope and suggestions for What to look at
I am not sure I have answer for your question about kids. I think they need to see something compelling. I am just starting to do some presentations - I will try to include some movies with music so they can see microorganisms moving like water bears and paramecium. I will also bring a microscope so...
- Sun Apr 14, 2019 12:05 am
- Forum: Microscopy accessories
- Topic: Rheinberg Filters
- Replies: 10
- Views: 14231
Re: Rheinberg Filters
I have purchased a set of the Rheinberg filters on ebay for $30 and I think they work great! I wrote an article on Rheinberg filters: https://www.canadiannaturephotographer.com/rberdan_Rheinberg_filters2017.html And I am including one photo of a ribbon worm below I took with a Rheinberg filters. I a...
- Sat Apr 13, 2019 11:38 pm
- Forum: Illumination Techniques
- Topic: Bright field, Darkfield, Phase Contrast and Rheinberg compared
- Replies: 2
- Views: 6535
Bright field, Darkfield, Phase Contrast and Rheinberg compared
Below are pictures from a recent article I posted on my web site showing different lighting techniques. Photos are of Wood by auto - fluorescence, Volvox by bright field, dark field, positive phase contrast, and Ribbon worm Rheinberg. Rheinberg filter set can be purchased on Ebay for about $30. Furt...
- Sat Apr 13, 2019 11:17 pm
- Forum: Pictures and Videos
- Topic: Tips on Buying a Microscope and suggestions for What to look at
- Replies: 16
- Views: 9979
Tips on Buying a Microscope and suggestions for What to look at
I just posted an article on my web site including tips for buying a new and used microscope, also why some one might want to own a microscope and some subjects they can look at. https://www.canadiannaturephotographer.com/guide_buying_microscope.html Comments welcome Photos include, rotifer, onion ro...