MicrobeHunter.com Microscopy Forum

The diagonal artifacts do not look like aliasing to me -- way too regular and not closely related to the image structure. It is strange how the strength of the artifact appears to be related to more gradual intensity gradients. If I understand, the Rising Sun has the finest pixel pitch and therefor...

The amazing SEM image [click on the thumbnail] at the bottom of this page: https://naturalhistory.museumwales.ac.uk/diatoms/browsespecies.php?-recid=3534 gives a good indication of the structural detail which we simply cannot image properly with any optical microscope. MichaelG. . Here’s a crop: . C...

The artifacts in the RS example do appear to be jagged diagonal lines as described. Is the article saying that the lens (presumably a photo eyepiece in this case) is too sharp for the 1" sensor? Yes, I think that’s effectively what they are saying, although it’s perhaps an unusual way of putting it...

David,

Sorry to be a pest, but may I suggest that, for clarity, you identify the three images in your post
... They appear to be in reverse order compared to those on the web page.

MichaelG.

.
P.S. __ I think you might find this interesting:
https://www.imatest.com/docs/nyquist-aliasing/

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That’s neat

MichaelG.

adomacro wrote: ↑

Mon Aug 31, 2020 11:41 pm

hello, sorry mistake, it is effectively epi-illumination or coaxial lighting. Thank you so much.

Very impressive results
Thank you for sharing them

MichaelG.

75RR wrote: ↑

Mon Aug 31, 2020 7:10 pm

.
I am going to take a wild guess and suggest Epi illumination

... and you would very probably be correct.

With all those lovely colours though; I thought it might be more than just that.

MichaelG.

Lo siento, no puedo traducir "depilluminación"

MichaelG.

75RR wrote: ↑

Mon Aug 31, 2020 3:37 pm

.
Shutter page from manual.
.

Wow ... I wonder what practical use there might be for 1/32000th second exposure

and what frame rate it can achieve in Electronic Shutter mode ?

MichaelG.

If his Olympus is giving the OP grief, my gut tells me a Panasonic mechanical shutter might not be an improvement. Did Panasonic ever tame their mechanical shutters or did they resort to using an electronic shutter at exposure times when it would most help? . I’m sure you are correct ... but that w...

Your dedication is rewarded, Hans

MichaelG.

Thanks, Jan ... very useful info.

MichaelG.

jfiresto wrote: ↑

Mon Aug 31, 2020 9:35 am

I think Olympus has always shown more interest than Panasonic in reducing mechanical shutter shock.

Very likely ^^^

I was, however, careful to write:

... ( I love my Lumix G1 ) but that mechanical shutter [*] ...

when citing it as an example.

Progress is certainly being made.

MichaelG.

Very happy to continue the discussion ... It’s a problem which many of us have to face, and this seems a good place to share ideas and experiences. Away from the specifics of 75RR’s illustrated system [see the opening post] there are several ‘touchstones’ for anyone designing/building a system: In t...

I believe 75RR already confirmed that vibration isolation between the camera and microscope (in the form of a tripod) works: . But both the optical configuration and the mechanical arrangement are different there ^^^ (i) There is a camera lens included (ii) The tripod adds mass and inertia to the c...

Elements of this story seem strangely familiar: http://www.microscopy-uk.org.uk/mag/artaug17/fp-camera.pdf ... although he appears more content with the results. I note the phrase “kicking like a mule” in relation to your camera model MichaelG. . Edit : I’ve now seen your contributions to this threa...

If, as has been suggested [and is probable], the vibration is induced by the camera shutter ... then neither (a) isolating the camera body from the microscope tube, nor (b) isolating the microscope from the floor is likely to bring much benefit. (a) would simply allow the camera body to move in rela...

34 tons makes a nice ‘mechanical earth’ but might be excessive for the typical amateur’s situation. Indeed! Was thinking marble floor tiles (slab was a bit of an exaggeration), the smaller ones tend to be 30 cm x 60 cm by 15mm or so. One cut in half should make a nice neoprene sandwich. It won’t do...

. Found this: https://www2.lbl.gov/Science-Articles/Archive/no-jiggles.html Concrete and neoprene according to them, sure I can get my hands on a couple of marble slabs as a concrete substitute, they seem to use them everywhere in Spain. Scuba diving suits are made of neoprene I believe. . Mmm ... ...

On the vibration isolation issue The best solution I have seen [short of a very expensive commercial table] is to put a go-cart inner tube between two heavy paving slabs. I probably can’t get to the relevant book for a while, but I will add a reference when I can. MichaelG. . Done: http://www.lester...

Indeed, that is why I ordered it. Always nice though when things (when they finally arrive) fit snugly when tested :) . [/quote] . Sorry, I obviously misinterpreted what you wrote ... I thought you were pleasantly surprised to find that Exakta was compatible with Ihagee. I bought a little Exa 500 n...

After a bit of head scratching and some internet searching I found what looked to be a potential solution, an Exakta EXA to M4/3 mount. Happy to say that when it arrived it fit both parts perfectly and therefore solved the problem. . Yes, the Exakta and Exa were made by Ihagee http://camera-wiki.or...

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Here’s a tidier version of my little spreadsheet
. .

As presented :

D7 = D3

C5 = (D3 x E4) / (E4 - D3)

E9 = (D7 x C8) / (C8 - D7)

and it’s showing 1:1 magnification
... which is a convenient check on the calculation formula

MichaelG.

All these AO/Reichert infinity bodies actually do focus by moving the objective, not the stage, so taking "focus needs to be raised" literally your original interpretation would be correct. . Thanks for confirming that, Hans Hopfully apochronaut can tell us what objective he uses Yes, my 5mm was ju...

. […] but: The focus needs to be raised about 200 microns. I think this is not a huge distance, but also not obviously negligible. Taking my 5mm lens, and the simple lens formula ... that would mean that the primary image lies at v=130mm instead of infinity. ... Presumably I have misinterpreted the...

I’m not sure how much this helps, but I have just been playing with the simple lens formula [ignoring the modern convention where u is negative] ... 1/u + 1/v = 1/f can be rearranged as u = (fv)/(v-f) ... which is easy to pop into a spreadsheet or calculator, for some spot checks. Taking a 5mm lens ...

I just need to catch up on some things before getting into it. It's a whole different direction from collecting and refurbishing microscopes but I think it could be just as much of an addiction! . This is a very useful ‘quick reference’ http://www.ftfarm.co.uk/microscopy/iceni/index.htm I particula...

BramHuntingNematodes wrote: ↑

Fri Aug 28, 2020 10:19 pm

No problem Nick

Embarrassing faux pas duly edited

MichaelG.

Thanks for sharing the images, Brad
Edit: With apologies Bram

Looks like it should be useful

MichaelG.

Katie,

I have just posted this link in another topic ... but I think you will find it very useful, so here it is:
https://www.quekett.org/wp-content/uplo ... camera.pdf

It’s a few years old, but highly relevant to your plans

MichaelG.