MicrobeHunter.com Microscopy Forum

What I would do is to remove the existing desk and replace it with a massive lab bench:

OK, so , i tmay be that moly grease seperates or maybe it doesn't, but moly is also extremely difficult to clean (this is also the reason it is desirable as a lubricant). I have recommended silicone grease for plastic gears before as it usually doesn't attack plastics. These greases will work fine f...

I cant remember why to not use moly now.

I use old white lithium grease. Don't use moly it can scratch i think? Also silicone grease is not preferred a true pain to try to clean off glass. It's not super duper critical to get the exact specific grease, just some things to think about.

Give me a harder one!

A fly's tongue

Still working on that but it's taking a little longer as I decided to build a clean hood for assembling the plates.

Have you been oiling it to the bottom of your slide?

(That's a very nice df condenser)

Ideally you want to sputter some metallic thin film on a plate as this would not have an effect on the phase of light that that penetrates. I worry if you somehow manage to cut two concentric rings from this material with a cutting plotter or whatever it's going to do something to the phase.of the s...

Thanks, Michael, for this fine combination of science and sweet prose ! Can we estimate its year of publication ? How do we translate the article's terminology to modern terms ? like, what is today's equivalent of a 1/8 lens, who sells "spotted" lenses or objectives, etc ? An 1/8 lens has a magnifi...

I wouldn't go out and say it's impossible but were dealing with some pretty small margins for error here. Careful gluing stuff to your phase plate as I imagine you will not get it right the first time and then you are out a perfectly good phase plate. Also, maybe reflection should be the principle o...

Welcome to the forum! I may consult with you on feasible amateur fluorescence someday. Field curvature correction, which I believe is treated separately from spherical aberration in lens design, is certainly very important in hematology. It is much less important when chasing down the thrashing nema...

I never had any luck with Hempstead halide euparal. My bottle has not worked at all. Might as well get a contemporary resin medium and a matching clearing agent. I use omnimount and histoclear 2. Barring that you could try like white glue. Tweezers are a good investment just get some nice ones with ...

small screwdrivers are fine you won't need any new parts since you won't lose any of these. If you can take apart a scroll saw and put it back together you can take apart a 1942 Dynoptic and I bet a Nikon S isn't too far removed from that. Best bet for the gears is that either they aren't broken. If...

I have a dry dark field condenser that should work until an NA of about .9. How was this effect achieved?

I got the old B&L dual gooseneck light works great very nice sheathing

If you like that stark hunk of machinery look there have been a few well-accoutered Zetopan microscopes I have seen sell here and there for less than $500. You might keep your eye out.

Oh yeah big time pretty common

Maybe if you are using an objective as your condenser it works like a darkfield stop

Were you holding your breath the whole time?

1998 is nearly brand new! Welcome. We could always use some more fluorescence expertise and I'm looking forward to your input.

Cycloptic!!

Magnificent creature

If the K has a phase turret that's a no brainer buy it. No need to make your own darkfield filters when you have a phase turret you will be able to achieve darkfield better than homemade filters with the annular diaphragms. You can have just a couple phase lenses. I often have bf lens s alongside ph...

There's a Nikon CFL objective inf. TL and coverslip corrected with a near 4mm wd. Look around maybe

A description of the use of the technique from 1903:

https://www.biodiversitylibrary.org/ite ... 6/mode/1up

the oscillating projection disc is an ancient technique discovered long ago and reintroduced it seems periodically. I think there must be some improvement but the mechanism probably breaks down frequently

I would go ahead and go 10 watt as long as you are going to bother. There are some very high CRI dies out there, all still with something of a spike in the high frequencies but much smoother than previous generations. You may be able to solder some leads into a star led that the halogen plug with th...

these are not good microscope LED dies. You want a single LED die. All the calculations are from a point source approximately the size of a filament i.e. a single small LED.

I get the impression that pond water organisms are continuously in flux as far as the most active species go depending on the environment. My son and were looking at some water from a bird bath as part of a school assignment and while rotifers were seen immediately after sampling, a few hours later ...